##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043343_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2266556
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22820349464121	33.0	31.0	34.0	31.0	34.0
2	32.522382416317974	34.0	31.0	34.0	31.0	34.0
3	32.63114434410621	34.0	31.0	34.0	31.0	34.0
4	36.13756245157852	37.0	35.0	37.0	35.0	37.0
5	36.11921655586714	37.0	35.0	37.0	35.0	37.0
6	36.29275208730779	37.0	37.0	37.0	35.0	37.0
7	36.22463376153071	37.0	37.0	37.0	35.0	37.0
8	36.12411605978409	37.0	37.0	37.0	35.0	37.0
9	37.69819497069563	39.0	38.0	39.0	35.0	39.0
10	37.567516972887496	39.0	37.0	39.0	35.0	39.0
11	37.41599016304914	39.0	37.0	39.0	35.0	39.0
12	37.43965690677839	39.0	37.0	39.0	35.0	39.0
13	37.4478503950487	39.0	37.0	39.0	35.0	39.0
14	38.68691265514728	40.0	38.0	41.0	35.0	41.0
15	38.816465598026255	40.0	38.0	41.0	35.0	41.0
16	38.80777355600303	40.0	38.0	41.0	35.0	41.0
17	38.70162440283849	40.0	38.0	41.0	34.0	41.0
18	38.56317426086097	40.0	38.0	41.0	35.0	41.0
19	38.360004782586444	40.0	37.0	41.0	35.0	41.0
20	37.9965304188381	40.0	35.0	41.0	34.0	41.0
21	37.99045644581471	40.0	35.0	41.0	34.0	41.0
22	37.98326315343631	40.0	35.0	41.0	34.0	41.0
23	37.95392745645817	40.0	35.0	41.0	34.0	41.0
24	37.889643582598445	40.0	35.0	41.0	34.0	41.0
25	37.843060572957384	40.0	35.0	41.0	34.0	41.0
26	37.82864133954775	40.0	35.0	41.0	34.0	41.0
27	37.796427266743024	40.0	35.0	41.0	34.0	41.0
28	37.700479052800816	40.0	35.0	41.0	34.0	41.0
29	37.720083686438805	40.0	35.0	41.0	34.0	41.0
30	37.624080763943184	40.0	35.0	41.0	34.0	41.0
31	37.49221947306839	40.0	35.0	41.0	33.0	41.0
32	37.27652703043737	40.0	35.0	41.0	33.0	41.0
33	36.89952200607441	40.0	35.0	41.0	32.0	41.0
34	36.69230453604499	39.0	35.0	41.0	31.0	41.0
35	36.58720013977153	39.0	35.0	41.0	31.0	41.0
36	36.50827069792231	39.0	35.0	41.0	31.0	41.0
37	36.37632028504921	39.0	35.0	41.0	31.0	41.0
38	36.246555567124744	39.0	35.0	41.0	31.0	41.0
39	36.173276989405956	39.0	35.0	41.0	31.0	41.0
40	36.052955232520176	39.0	35.0	41.0	30.0	41.0
41	35.96515550465111	38.0	35.0	41.0	30.0	41.0
42	35.87161579065331	38.0	35.0	41.0	30.0	41.0
43	35.74723457086434	38.0	35.0	40.0	30.0	41.0
44	35.60442318654381	38.0	35.0	40.0	29.0	41.0
45	35.51705847991402	38.0	35.0	40.0	29.0	41.0
46	35.402845550694536	37.0	35.0	40.0	28.0	41.0
47	35.25859100767861	37.0	35.0	40.0	28.0	41.0
48	35.1871756091621	37.0	35.0	40.0	28.0	41.0
49	35.10064123718981	37.0	35.0	40.0	28.0	41.0
50	35.015642675495336	37.0	35.0	40.0	28.0	41.0
51	34.598017432615826	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	2.0
12	5.0
13	4.0
14	6.0
15	23.0
16	75.0
17	171.0
18	371.0
19	736.0
20	1334.0
21	2216.0
22	3703.0
23	5678.0
24	9678.0
25	16714.0
26	26104.0
27	33370.0
28	35301.0
29	35364.0
30	36922.0
31	42047.0
32	49814.0
33	65990.0
34	128876.0
35	193334.0
36	221308.0
37	238417.0
38	351544.0
39	767217.0
40	230.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.592336567020624	17.329110774231918	22.9585326813015	28.12001997744596
2	35.22913177525726	19.827835711978878	23.22214849313231	21.720884019631548
3	27.00202421647645	20.676524206770093	32.40299379322637	19.918457783527078
4	23.730496841904632	23.420290520066565	30.37427709705827	22.47493554097053
5	21.951807058815227	31.471889509899604	27.698764116130377	18.8775393151548
6	77.1943424296598	2.3270547914986435	17.30444780539285	3.1741549734487036
7	76.35725744256926	2.5596985029269077	15.599967527826358	5.483076526677479
8	65.29474674351748	4.217102952673572	22.238541646445093	8.24960865736386
9	38.31398827119206	15.528361090570892	26.834854289944744	19.322796348292297
10	24.96399824226712	15.809580703057854	36.037053573792136	23.18936748088289
11	21.850419755788078	13.212821567170632	39.87066721492873	25.06609146211256
12	21.069411035950576	14.83409189978099	42.49835433141736	21.598142732851073
13	18.305129015122503	18.344351518338836	42.45079318578495	20.899726280753708
14	15.239949950497584	18.617982525029163	44.08507885973256	22.05698866474069
15	18.302702426059625	16.938297575705167	42.32077213181585	22.43822786641936
16	19.383328715460813	19.762714885491466	37.11644450876131	23.73751189028641
17	18.192138204394684	19.547013177702205	39.448793676397145	22.81205494150597
18	21.854478777493256	23.89916684167521	35.39793413443127	18.848420246400266
19	22.211407968742	19.78358355143222	33.33118616967769	24.673822310148083
20	20.22001662434107	24.135516616399507	35.91206217715335	19.732404582106067
21	20.628036545313684	19.219997211628566	34.14449058395204	26.00747565910571
22	20.653449550772184	21.397441757450512	32.04518220595476	25.90392648582254
23	18.754489189766325	19.72411005949114	36.581271320893904	24.940129429848636
24	24.315922483274182	18.265862392105024	33.18276715863186	24.235447965988925
25	17.8615044146273	20.329169012369427	34.703885542647086	27.105441030356186
26	18.982765040881407	20.258533210739113	34.550260395066346	26.208441353313134
27	22.53326191808188	19.70116776289666	34.156182331254996	23.60938798776646
28	21.939850592705408	21.939365274892833	30.98935124479607	25.131432887605687
29	23.667979083684674	18.937762843715312	32.540162254980686	24.85409581761933
30	23.979508999557037	19.69282912048059	29.163629753687974	27.1640321262744
31	26.847825511480856	21.707383360481717	28.562938661122868	22.881852466914562
32	25.047869984240407	18.909879129392788	31.24952571213771	24.792725174229094
33	22.62820773014212	20.73511530268831	33.46729575620457	23.169381210965007
34	20.364067775073725	24.65780682233309	32.15817301668258	22.819952385910604
35	25.214466353357253	22.061753603264158	32.994949165165124	19.728830878213465
36	24.453135064829638	22.43262465167417	32.143039924890445	20.971200358605742
37	21.54321358042775	26.12761387761873	33.21854831735902	19.110624224594496
38	19.47443610482159	21.72979621946248	34.75581454859267	24.039953127123265
39	23.149041982637975	18.58859873746777	39.28096195284829	18.98139732704597
40	19.194848924976927	18.061543593010718	39.42002756605175	23.3235799159606
41	22.66606251952301	22.635266898325035	31.31102871493138	23.387641867220577
42	20.3026088920812	17.45498456689356	34.82040593746636	27.422000603558878
43	23.61313817086364	19.467244577235242	35.25154463423802	21.668072617663096
44	23.050566586486283	20.23722334678693	33.680350275925235	23.03185979080155
45	23.012976515912246	21.313128817465792	32.31594542557078	23.35794924105118
46	23.122658341554324	20.939698820589474	32.81361678246644	23.124026055389763
47	21.338762421930014	20.011506444138156	35.45096613540543	23.1987649985264
48	22.416300325251175	18.57020078039104	32.118862273863954	26.894636620493824
49	21.1879168218213	18.67908844961254	36.376952521799595	23.756042206766566
50	21.78997562822185	16.81904175321501	36.777207357771	24.613775260792146
51	20.69461332523882	16.509408988791805	35.500953870100716	27.295023815868657
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	490.0
1	3615.0
2	6740.0
3	63692.5
4	120645.0
5	68520.5
6	16396.0
7	15056.5
8	13717.0
9	13356.0
10	12995.0
11	12806.5
12	12618.0
13	12054.0
14	11490.0
15	10683.5
16	9877.0
17	9703.0
18	9529.0
19	8900.5
20	8272.0
21	8022.5
22	7773.0
23	7477.0
24	7181.0
25	7406.0
26	8645.5
27	9660.0
28	10036.5
29	10413.0
30	12588.5
31	14764.0
32	16235.5
33	17707.0
34	19682.5
35	21658.0
36	24352.5
37	27047.0
38	30701.5
39	34356.0
40	42986.0
41	51616.0
42	64582.5
43	77549.0
44	108985.0
45	140421.0
46	184216.5
47	228012.0
48	252622.0
49	277232.0
50	239799.5
51	202367.0
52	170944.5
53	139522.0
54	127412.0
55	115302.0
56	110541.0
57	105780.0
58	103989.5
59	102199.0
60	96572.0
61	90945.0
62	90674.0
63	90403.0
64	87410.0
65	84417.0
66	72907.5
67	61398.0
68	49960.0
69	38522.0
70	32184.5
71	25847.0
72	23858.5
73	21870.0
74	18418.0
75	11857.0
76	8748.0
77	6566.0
78	4384.0
79	3281.5
80	2179.0
81	1618.0
82	1057.0
83	856.5
84	656.0
85	384.0
86	112.0
87	84.5
88	57.0
89	36.5
90	16.0
91	13.5
92	11.0
93	9.5
94	8.0
95	4.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2266556.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.895332872685014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.18925976361977	20.137434309389
2	11.43015687869717	6.376960618365358
3	4.482543721761573	3.7512614770471018
4	2.2401062360758686	2.4995403650205548
5	1.3328431043643856	1.8590051031653694
6	0.8768885248652835	1.467665837601289
7	0.6727794713690984	1.313718511262504
8	0.4898460541299941	1.0931534989061973
9	0.42436433303293614	1.0654005896323842
>10	5.2498782116787055	32.89510234880682
>50	0.4287414803818862	7.926585167103256
>100	0.1679213860382231	8.49646643562965
>500	0.007654348166162207	1.426184090954958
>1k	0.006059692298211747	3.322113479950662
>5k	4.7839676038513796E-4	1.034106907533449
>10k+	4.7839676038513796E-4	5.335301259631536
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	97964	4.322152199195608	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	11773	0.5194224188592736	No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	10202	0.4501102112632558	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	9361	0.41300545850179743	Illumina PCR Primer Index 5 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	7311	0.3225598661581713	Illumina PCR Primer Index 5 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	6575	0.2900876925167523	No Hit
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG	4934	0.2176870988407081	Illumina PCR Primer Index 5 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC	3926	0.17321433928832997	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3269	0.14422762993722635	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3182	0.14038920723776513	No Hit
AGCCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC	3071	0.13549190931086635	No Hit
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	2925	0.12905041834395445	Illumina PCR Primer Index 5 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG	2828	0.1247707976330609	Illumina PCR Primer Index 5 (95% over 22bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	2786	0.12291776598504515	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC	2567	0.11325552953467728	No Hit
AGGCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC	2385	0.105225725726609	No Hit
GGGCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC	2382	0.10509336632317931	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.42734439387334794	0.0
2	0.0	0.0	0.0	1.430408072864734	0.0
3	0.0	0.0	0.0	2.3057449275464625	0.0
4	0.0	0.0	0.0	2.920774955483121	0.0
5	0.0	0.0	0.0	6.029014946023835	0.0
6	0.0	0.0	0.0	6.896189637494066	0.0
7	0.0	0.0	0.0	9.764285550412167	0.0
8	0.0	0.0	0.0	12.869481274674	0.0
9	0.0	0.0	0.0	15.59727621995662	0.0
10	0.0	0.0	0.0	17.250312809390106	0.0
11	0.0	0.0	0.0	18.76234251436982	0.0
12	0.0	0.0	0.0	19.835027239565225	0.0
13	0.0	0.0	0.0	20.33221327864831	0.0
14	0.0	0.0	0.0	20.694392726233104	0.0
15	0.0	0.0	0.0	21.78225466302178	0.0
16	0.0	0.0	0.0	22.88719096285289	0.0
17	0.0	0.0	0.0	24.331364413674315	0.0
18	0.0	0.0	0.0	25.065032586885124	0.0
19	0.0	0.0	0.0	25.92832473585475	0.0
20	0.0	0.0	0.0	26.903637059927043	0.0
21	0.0	0.0	0.0	27.85141862808596	0.0
22	4.411980114323229E-5	0.0	0.0	28.67994437375472	0.0
23	4.411980114323229E-5	0.0	0.0	29.459541259955632	0.0
24	2.2059900571616144E-4	0.0	0.0	30.072630016641988	0.0
25	2.2059900571616144E-4	0.0	0.0	30.688983638612942	0.0
26	2.2059900571616144E-4	0.0	0.0	31.256143682309194	0.0
27	2.2059900571616144E-4	0.0	0.0	31.836936744558706	0.0
28	3.0883860800262603E-4	0.0	0.0	32.40171431899322	0.0
29	3.0883860800262603E-4	0.0	0.0	33.000331780904595	0.0
30	3.0883860800262603E-4	0.0	0.0	33.58452206784214	0.0
31	3.0883860800262603E-4	0.0	0.0	34.15882951932359	0.0
32	3.0883860800262603E-4	0.0	0.0	34.7761537769197	0.0
33	3.0883860800262603E-4	0.0	0.0	35.31084164697453	0.0
34	3.529584091458583E-4	0.0	0.0	35.91470936522195	0.0
35	3.529584091458583E-4	0.0	0.0	36.43616129493381	0.0
36	3.529584091458583E-4	0.0	0.0	37.038881898351505	0.0
37	3.529584091458583E-4	0.0	0.0	37.64469088784923	0.0
38	3.529584091458583E-4	0.0	0.0	38.39388923106246	0.0
39	3.529584091458583E-4	0.0	0.0	39.06455432824073	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTA	20	7.0347375E-4	45.000004	18
CTGCGTA	25	3.8920112E-5	45.0	24
AATGCGC	35	1.2126293E-7	45.0	9
CGTAAGG	30	2.16644E-6	44.999996	1
CGTTTTT	22935	0.0	44.215176	1
TACGCGG	190	0.0	43.81579	1
TACGGGT	175	0.0	41.142857	3
CTTACGG	280	0.0	40.98214	1
CACTTAC	3105	0.0	40.7971	36
CCGCACT	3120	0.0	40.673077	33
CGCACTT	3140	0.0	40.628983	34
TACGGGA	305	0.0	40.573772	3
TAGTAGG	505	0.0	40.544556	1
GCACTTA	3130	0.0	40.543133	35
TAGGGTA	640	0.0	40.42969	4
ACGGGTA	195	0.0	40.384617	4
ACTTACT	3090	0.0	40.26699	37
TCACGAC	375	0.0	40.199997	24
ACCCGCA	3220	0.0	40.178574	31
CACGACG	370	0.0	40.135136	25
>>END_MODULE