FastQCFastQC Report
Thu 26 May 2016
SRR1043342_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043342_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2259429
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1153525.10536069068778No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT104670.4632586374699094No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC100590.445200977769162No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCTT95070.4207700264093273Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA64530.2856031324728504No Hit
CGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC64070.2835672198595309No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT54540.24138842158793217No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT45850.20292737678413442No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCG39470.1746901540167892No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC37740.16703335223191346No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT31580.13976982680137326No Hit
GGGCCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT30990.13715854757994167No Hit
CGTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG27790.12299567722641427No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCT24490.10839021717433918No Hit
TCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT24490.10839021717433918No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTC206.312756E-446.08
CGTTTTT283050.045.341811
ACGTAGG1650.043.2121241
CTTACGG2150.042.79071
AATACGG650.042.461541
TCGGGTA601.8189894E-1242.1666684
TCAAGCG4700.042.08510616
TATGCGG3400.041.9411771
AATGCGG3400.041.9411771
ACTACGG1900.041.1578941
GTTTTTT318150.041.0913122
CACTTAC25150.040.8787336
ACCCGCA25850.040.75048431
TTAACGG850.040.5882341
ATACGAA1700.040.58823418
ACATACG1700.040.58823416
CTCACGA2500.040.47999623
CGCACTT25400.040.4763834
GTCCCCC11650.040.4721039
TAGCATA8700.040.44827729
Produced by FastQC (version 0.11.5)