##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043342_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2259429
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.332599077023445	33.0	31.0	34.0	31.0	34.0
2	32.65648002216489	34.0	31.0	34.0	31.0	34.0
3	32.75143631421921	34.0	31.0	34.0	31.0	34.0
4	36.20268395244993	37.0	37.0	37.0	35.0	37.0
5	36.124596081576364	37.0	37.0	37.0	35.0	37.0
6	36.31085862844108	37.0	37.0	37.0	35.0	37.0
7	36.24681368611273	37.0	37.0	37.0	35.0	37.0
8	36.225514942049514	37.0	37.0	37.0	35.0	37.0
9	37.83074263453288	39.0	38.0	39.0	35.0	39.0
10	37.614374693783255	39.0	37.0	39.0	35.0	39.0
11	37.180201723532804	39.0	35.0	39.0	34.0	39.0
12	37.15624611350921	39.0	37.0	39.0	34.0	39.0
13	37.112662092944724	39.0	35.0	39.0	34.0	39.0
14	38.37964193608208	40.0	37.0	41.0	34.0	41.0
15	38.446306566836135	40.0	37.0	41.0	34.0	41.0
16	38.307011638781304	40.0	37.0	41.0	33.0	41.0
17	38.28390270285103	40.0	37.0	41.0	33.0	41.0
18	38.066846092530454	39.0	36.0	41.0	34.0	41.0
19	37.83469584572031	39.0	36.0	41.0	34.0	41.0
20	37.527176113965076	39.0	35.0	41.0	34.0	41.0
21	37.45499194708044	39.0	35.0	41.0	33.0	41.0
22	37.41610424580724	39.0	35.0	41.0	33.0	41.0
23	37.426527675797736	39.0	35.0	41.0	34.0	41.0
24	37.36474038352168	39.0	35.0	41.0	34.0	41.0
25	37.25850336523077	39.0	35.0	41.0	33.0	41.0
26	37.185002493992954	39.0	35.0	41.0	33.0	41.0
27	37.21867117754088	39.0	35.0	41.0	33.0	41.0
28	37.20810080777046	39.0	35.0	41.0	33.0	41.0
29	37.10677476477464	39.0	35.0	41.0	33.0	41.0
30	37.033504482769764	39.0	35.0	41.0	33.0	41.0
31	36.90428333884358	39.0	35.0	41.0	33.0	41.0
32	36.648811270458154	39.0	35.0	41.0	32.0	41.0
33	36.235779039748536	39.0	35.0	41.0	31.0	41.0
34	36.00818835201283	38.0	35.0	41.0	30.0	41.0
35	35.8311334412367	38.0	35.0	41.0	29.0	41.0
36	35.79201913403785	38.0	35.0	41.0	30.0	41.0
37	35.72421881811732	38.0	35.0	41.0	29.0	41.0
38	35.624080243282705	38.0	35.0	41.0	29.0	41.0
39	35.61503370984439	38.0	35.0	41.0	29.0	41.0
40	35.56723313722184	38.0	35.0	41.0	29.0	41.0
41	35.53809126111066	38.0	35.0	41.0	29.0	41.0
42	35.453095007632456	37.0	35.0	41.0	29.0	41.0
43	35.34596484332989	37.0	35.0	40.0	29.0	41.0
44	35.17378859880085	37.0	35.0	40.0	27.0	41.0
45	35.0774080530966	37.0	35.0	40.0	26.0	41.0
46	35.048394085408304	37.0	35.0	40.0	26.0	41.0
47	34.978321071385736	36.0	35.0	40.0	26.0	41.0
48	34.89126146473291	36.0	35.0	40.0	26.0	41.0
49	34.84298378041532	36.0	35.0	40.0	27.0	41.0
50	34.739044687839275	36.0	35.0	40.0	26.0	41.0
51	34.624714474320726	35.0	35.0	40.0	26.0	41.0
52	34.254450128771474	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	4.0
13	2.0
14	21.0
15	46.0
16	92.0
17	258.0
18	510.0
19	971.0
20	1852.0
21	2937.0
22	4609.0
23	7286.0
24	12471.0
25	21844.0
26	32242.0
27	38699.0
28	40664.0
29	41154.0
30	44107.0
31	49064.0
32	57950.0
33	74975.0
34	180677.0
35	228673.0
36	215000.0
37	197691.0
38	289044.0
39	713916.0
40	2664.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.774482402412293	16.842219870595624	22.876974669263785	31.506323057728302
2	36.54533955260378	18.879415994040972	23.59335035533314	20.98189409802211
3	25.3390126443451	20.39178925294842	35.1606976806972	19.10850042200928
4	23.323105085399895	22.58393602985533	33.080304802673595	21.012654082071176
5	21.547125402037416	29.873786695665146	30.41122336661165	18.167864535685784
6	75.46083545887036	1.9635049386371513	19.316915911055403	3.258743691437084
7	75.11340254551038	1.781423536654615	17.628834541824506	5.476339376010488
8	65.4086497075146	3.2678610392271676	23.6885071405209	7.634982112737333
9	42.600409218435274	13.40506827167395	26.869487821923148	17.125034687967624
10	30.603971180329186	14.363363486969497	36.4560692104067	18.576596122294614
11	27.162747756180877	11.900440332491085	39.01113954012275	21.92567237120529
12	22.27461008954032	13.132477276338403	42.18596822471518	22.406944409406094
13	17.19129036584022	15.872196028288563	44.83880661884042	22.097706987030794
14	17.3237132036457	16.327133979425774	44.632736855196605	21.716415961731926
15	21.334593828794798	14.336852364026488	43.24251835308833	21.08603545409039
16	20.171556618951072	18.707823967914017	40.217152209695456	20.90346720343945
17	20.792023117345135	19.21543009317841	39.83046159007431	20.16208519940215
18	20.23276677426022	25.708309488813324	36.38618429700601	17.67273943992044
19	22.123244412636996	21.767800625733315	34.098084073453954	22.01087088817573
20	20.37497084440361	26.876215185341074	34.48442062131627	18.264393348939045
21	18.580667947521253	20.674736847229987	36.89184302759679	23.852752177651965
22	20.575906567544276	21.883139501174853	33.85952822593673	23.68142570534414
23	21.315119882058696	19.200293525488078	37.2359122592478	22.248674333205425
24	23.59742218055978	17.54040512005467	34.07958382405466	24.78258887533089
25	17.483620861730994	18.384069603426354	38.16663413632382	25.96567539851883
26	16.51311017075553	17.727664821510213	37.66394075671331	28.095284251020942
27	20.520538596255957	20.841062055944224	36.2752270595801	22.363172288219722
28	22.32878306864256	21.559739208446025	32.310110209260834	23.801367513650572
29	22.419292661995573	18.97815775578697	33.998280096431444	24.60426948578601
30	26.058619235213854	18.059075987782755	32.78766449399384	23.094640283009557
31	25.879193371422605	20.1208358394975	29.67612613629373	24.32384465278617
32	25.93916427557582	18.927038645604707	30.88324528011281	24.250551798706667
33	21.363937525808513	20.82942194687242	35.93111356895924	21.875526958359835
34	21.228947667751456	25.70335248418959	33.05149221329814	20.016207634760818
35	24.794317502342405	27.99260344095787	29.130855627682923	18.0822234290168
36	26.22282001337506	25.54795924102948	29.573799397989493	18.655421347605966
37	23.594412570609656	30.05763845644187	29.53020431268254	16.817744660265934
38	21.768553028220847	26.201531448874917	30.1144669737354	21.91544854916884
39	22.50351748162921	22.109656908891584	34.80228854281325	20.58453706666596
40	18.59620284594028	20.52939039022691	37.57055433031974	23.303852433513068
41	19.54480534683763	23.15585043831871	31.780551634948477	25.518792579895187
42	19.19002544448177	18.249920665796534	35.153394950671164	27.406658939050534
43	22.600002035912613	19.18161624020936	34.46653999749494	23.751841726383084
44	22.03286759619355	20.065113796450344	34.47410828134011	23.427910326015997
45	21.974844086713944	21.69959755318711	33.110445161144696	23.21511319895425
46	22.26199628313171	20.248832780317507	33.30350278765122	24.185668148899566
47	20.240556352954663	20.013773391418805	35.51512351129423	24.230546744332308
48	22.041586613254943	18.27519253758361	32.49723713380682	27.185983715354634
49	21.079839198310722	17.17579972639105	36.58557095620177	25.158790119096462
50	20.28149590007033	17.05032554685277	37.94724242275371	24.720936130323192
51	18.569160615359014	15.962971175460702	37.0852104668923	28.382657742287986
52	16.25800146851262	16.744540324126138	41.787814531901645	25.2096436754596
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	569.0
1	3567.5
2	6566.0
3	81565.5
4	156565.0
5	88265.0
6	19965.0
7	17803.5
8	15642.0
9	15137.0
10	14632.0
11	14232.0
12	13832.0
13	13219.0
14	11654.0
15	10702.0
16	10254.0
17	9806.0
18	9088.5
19	8371.0
20	8038.0
21	7705.0
22	7338.5
23	6972.0
24	7208.5
25	7445.0
26	8216.5
27	8988.0
28	8697.0
29	8406.0
30	9113.0
31	9820.0
32	11043.0
33	12266.0
34	14544.0
35	16822.0
36	19103.0
37	21384.0
38	24062.0
39	30115.5
40	33491.0
41	45571.0
42	57651.0
43	81989.0
44	106327.0
45	146352.5
46	186378.0
47	207358.0
48	228338.0
49	221780.0
50	215222.0
51	193252.0
52	171282.0
53	151509.5
54	131737.0
55	123671.0
56	115605.0
57	112282.5
58	108960.0
59	106161.0
60	103362.0
61	101069.0
62	98776.0
63	97679.0
64	83166.0
65	69750.0
66	58140.5
67	46531.0
68	37968.0
69	29405.0
70	24608.5
71	19812.0
72	19305.0
73	18798.0
74	15322.0
75	11846.0
76	9204.0
77	6562.0
78	5035.5
79	3509.0
80	2634.0
81	1759.0
82	1485.0
83	1211.0
84	829.5
85	448.0
86	288.5
87	129.0
88	113.0
89	70.0
90	43.0
91	22.5
92	2.0
93	4.5
94	7.0
95	5.5
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2259429.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.773915940839995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.91098006344983	22.745302875777057
2	10.733568679180085	6.606278805566418
3	3.9250391592170524	3.623664754507526
4	2.033699490183327	2.5033958863932346
5	1.2655540924537407	1.9473027629878732
6	0.9250439936894358	1.7080335662026571
7	0.705490122396133	1.519748560659806
8	0.5891925740616452	1.4505410197712167
9	0.5147636229970619	1.4257163217162452
>10	4.96730202926036	30.52202473927596
>50	0.2558311011865723	5.387161781399106
>100	0.1604196241896772	8.935379817673034
>500	0.007062164164996848	1.4509690250367082
>1k	0.005044402974997749	2.913913401200148
>5k	5.765031971425999E-4	1.2339490507289312
>10k+	4.323773978569499E-4	6.026617631104048
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	115352	5.10536069068778	No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT	10467	0.4632586374699094	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	10059	0.445200977769162	No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCTT	9507	0.4207700264093273	Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	6453	0.2856031324728504	No Hit
CGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	6407	0.2835672198595309	No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT	5454	0.24138842158793217	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT	4585	0.20292737678413442	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCG	3947	0.1746901540167892	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC	3774	0.16703335223191346	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT	3158	0.13976982680137326	No Hit
GGGCCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT	3099	0.13715854757994167	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG	2779	0.12299567722641427	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCT	2449	0.10839021717433918	No Hit
TCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT	2449	0.10839021717433918	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4352427095518381	0.0
2	0.0	0.0	0.0	1.3333014668750378	0.0
3	0.0	0.0	0.0	2.1696632202206843	0.0
4	0.0	0.0	0.0	2.7639284084607216	0.0
5	0.0	0.0	0.0	5.535779172525448	0.0
6	0.0	0.0	0.0	6.674252654099774	0.0
7	0.0	0.0	0.0	9.775434412853867	0.0
8	0.0	0.0	0.0	13.360455230060339	0.0
9	0.0	0.0	0.0	16.701741900276573	0.0
10	0.0	0.0	0.0	18.546013174124965	0.0
11	0.0	0.0	0.0	20.048915013483494	0.0
12	0.0	0.0	0.0	21.112590836003257	0.0
13	0.0	0.0	0.0	21.678574542506095	0.0
14	0.0	0.0	0.0	22.025830419986644	0.0
15	0.0	0.0	0.0	22.839531580766646	0.0
16	0.0	0.0	0.0	23.701961867356754	0.0
17	0.0	0.0	0.0	24.788740872140703	0.0
18	0.0	0.0	0.0	25.46430978800396	4.425896985477304E-5
19	0.0	0.0	0.0	26.20148718990506	4.425896985477304E-5
20	0.0	0.0	0.0	27.021119052645602	4.425896985477304E-5
21	0.0	0.0	0.0	27.84154757684353	4.425896985477304E-5
22	2.212948492738652E-4	0.0	0.0	28.582664027061703	4.425896985477304E-5
23	2.212948492738652E-4	0.0	0.0	29.288329042426206	4.425896985477304E-5
24	2.212948492738652E-4	0.0	0.0	29.856746992271056	4.425896985477304E-5
25	2.212948492738652E-4	0.0	0.0	30.41759665827074	4.425896985477304E-5
26	2.212948492738652E-4	0.0	0.0	30.96977156617889	4.425896985477304E-5
27	2.212948492738652E-4	0.0	0.0	31.520441669111975	4.425896985477304E-5
28	2.212948492738652E-4	0.0	0.0	32.0413254853328	4.425896985477304E-5
29	2.212948492738652E-4	0.0	0.0	32.59876721065366	4.425896985477304E-5
30	2.212948492738652E-4	0.0	0.0	33.13022892066978	4.425896985477304E-5
31	2.212948492738652E-4	0.0	0.0	33.66394783814849	4.425896985477304E-5
32	2.212948492738652E-4	0.0	0.0	34.24763513259323	4.425896985477304E-5
33	2.212948492738652E-4	0.0	0.0	34.783345703715405	4.425896985477304E-5
34	2.212948492738652E-4	0.0	0.0	35.390932841881735	4.425896985477304E-5
35	2.6555381912863826E-4	0.0	0.0	35.917171993454986	4.425896985477304E-5
36	2.6555381912863826E-4	0.0	0.0	36.4848375408123	4.425896985477304E-5
37	2.6555381912863826E-4	0.0	0.0	37.23905464610749	4.425896985477304E-5
38	2.6555381912863826E-4	0.0	0.0	38.0213319382906	4.425896985477304E-5
39	2.6555381912863826E-4	0.0	0.0	38.87614968206569	4.425896985477304E-5
40	3.098127889834113E-4	0.0	0.0	39.57991156172643	4.425896985477304E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGTC	20	6.312756E-4	46.0	8
CGTTTTT	28305	0.0	45.34181	1
ACGTAGG	165	0.0	43.212124	1
CTTACGG	215	0.0	42.7907	1
AATACGG	65	0.0	42.46154	1
TCGGGTA	60	1.8189894E-12	42.166668	4
TCAAGCG	470	0.0	42.085106	16
TATGCGG	340	0.0	41.941177	1
AATGCGG	340	0.0	41.941177	1
ACTACGG	190	0.0	41.157894	1
GTTTTTT	31815	0.0	41.091312	2
CACTTAC	2515	0.0	40.87873	36
ACCCGCA	2585	0.0	40.750484	31
TTAACGG	85	0.0	40.588234	1
ATACGAA	170	0.0	40.588234	18
ACATACG	170	0.0	40.588234	16
CTCACGA	250	0.0	40.479996	23
CGCACTT	2540	0.0	40.47638	34
GTCCCCC	1165	0.0	40.472103	9
TAGCATA	870	0.0	40.448277	29
>>END_MODULE