FastQCFastQC Report
Thu 26 May 2016
SRR1043341_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043341_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2224240
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1091394.906799625939646No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC99120.44563536309031404No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC98190.4414541596230622No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT93500.42036830557853466No Hit
CGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG60360.2713735927777578No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG55440.2492536776606841No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC55120.24781498399453297No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC43850.19714599143977268No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCC37480.16850699564795166No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT35600.16005467035931376No Hit
GGGCCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC31150.14004783656439954No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCT30510.13717044923209726No Hit
CGTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT26230.11792792144732582No Hit
TCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC24060.10817178002373845No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTC23490.10560910693090672No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG253.891986E-545.00000435
TATCGTG207.0347096E-445.00000411
CGTTTTT273600.044.54771
TCACGAC2400.043.12500424
ATTACGG1700.041.0294151
TCGGGTA556.184564E-1140.9090924
ACGGGTA1550.040.645164
AATACGG501.0822987E-940.5000041
GTTTTTT306750.040.386312
CTAGCGG5650.040.2212371
CACTTAC25000.040.14000336
GCACTTA24950.040.0400835
CGCACTT24900.040.0301234
TAAACGG900.040.0000041
CGTTCTG5900.039.661021
TATACGG1250.039.6000021
CCCGCAC25500.039.5294132
CGGTCTA2450.039.48979630
CTTACTG25200.039.37499638
ACCCGCA25500.039.35294331