##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043341_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2224240
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29941598028989	33.0	31.0	34.0	31.0	34.0
2	32.58488787181239	34.0	31.0	34.0	31.0	34.0
3	32.66281291587239	34.0	31.0	34.0	31.0	34.0
4	36.169286587778295	37.0	37.0	37.0	35.0	37.0
5	36.13958700499946	37.0	37.0	37.0	35.0	37.0
6	36.32631775347984	37.0	37.0	37.0	35.0	37.0
7	36.27170179477035	37.0	37.0	37.0	35.0	37.0
8	36.178442524188036	37.0	37.0	37.0	35.0	37.0
9	37.79027533000036	39.0	38.0	39.0	35.0	39.0
10	37.55930295291875	39.0	37.0	39.0	35.0	39.0
11	37.17309373089235	39.0	35.0	39.0	34.0	39.0
12	37.157578768478224	39.0	37.0	39.0	34.0	39.0
13	37.12700338093011	39.0	35.0	39.0	34.0	39.0
14	38.39036614753804	40.0	37.0	41.0	34.0	41.0
15	38.46994344135525	40.0	37.0	41.0	34.0	41.0
16	38.37347858144805	40.0	37.0	41.0	34.0	41.0
17	38.32070729777362	40.0	37.0	41.0	34.0	41.0
18	38.12737879005863	40.0	37.0	41.0	34.0	41.0
19	37.91946507571125	40.0	36.0	41.0	34.0	41.0
20	37.63274781498399	39.0	35.0	41.0	34.0	41.0
21	37.56723644930403	39.0	35.0	41.0	34.0	41.0
22	37.54603549976621	39.0	35.0	41.0	34.0	41.0
23	37.524322015609826	39.0	35.0	41.0	34.0	41.0
24	37.464782577419705	39.0	35.0	41.0	34.0	41.0
25	37.42525941445168	39.0	35.0	41.0	34.0	41.0
26	37.41047458907312	39.0	35.0	41.0	34.0	41.0
27	37.38517695932094	39.0	35.0	41.0	34.0	41.0
28	37.31711775707657	39.0	35.0	41.0	34.0	41.0
29	37.250332697910295	39.0	35.0	41.0	33.0	41.0
30	37.1324227601338	39.0	35.0	41.0	33.0	41.0
31	36.99309112326008	39.0	35.0	41.0	33.0	41.0
32	36.75061953745999	39.0	35.0	41.0	33.0	41.0
33	36.336874617846995	39.0	35.0	41.0	31.0	41.0
34	36.059373988418514	38.0	35.0	41.0	30.0	41.0
35	35.87594054598425	38.0	35.0	41.0	30.0	41.0
36	35.805908085458405	38.0	35.0	41.0	30.0	41.0
37	35.6934602381038	38.0	35.0	41.0	29.0	41.0
38	35.588027371147	38.0	35.0	41.0	29.0	41.0
39	35.5340170125526	38.0	35.0	41.0	29.0	41.0
40	35.46656610797396	38.0	35.0	40.0	29.0	41.0
41	35.398601769593206	37.0	35.0	40.0	29.0	41.0
42	35.297216127755995	37.0	35.0	40.0	28.0	41.0
43	35.1832720389886	37.0	35.0	40.0	27.0	41.0
44	35.06714877890875	37.0	35.0	40.0	27.0	41.0
45	35.00376488148761	37.0	35.0	40.0	26.0	41.0
46	34.91508065676366	36.0	35.0	40.0	26.0	41.0
47	34.79104593029529	36.0	35.0	40.0	25.0	41.0
48	34.736358036902494	36.0	35.0	40.0	25.0	41.0
49	34.67389715138654	36.0	35.0	40.0	26.0	41.0
50	34.593741232960475	36.0	35.0	40.0	25.0	41.0
51	34.175044059993525	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	5.0
14	20.0
15	50.0
16	106.0
17	231.0
18	464.0
19	936.0
20	1687.0
21	2681.0
22	4182.0
23	6506.0
24	10805.0
25	18985.0
26	29706.0
27	37856.0
28	40458.0
29	40908.0
30	43631.0
31	48668.0
32	56215.0
33	73082.0
34	175007.0
35	231380.0
36	204066.0
37	196545.0
38	302405.0
39	697424.0
40	228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.027353163327696	16.901413516526993	23.030068697622557	31.04116462252275
2	36.24977520411466	18.956317663561485	23.632027119375607	21.16188001294824
3	25.366507211452	20.501294824299535	34.833606085674205	19.298591878574253
4	23.384391972089343	22.779466244649857	32.6887386253282	21.147403157932597
5	21.69568032226738	30.009081753767582	30.066629500413622	18.228608423551414
6	75.82873251088013	1.9533863252167032	18.945662338596556	3.2722188253066213
7	75.55735891810237	1.744416070208251	17.188118188684676	5.510106823004712
8	65.76934143797432	3.1796928389022767	23.3214940833723	7.7294716397511065
9	42.852479948207026	13.384077257849873	26.505817717512496	17.257625076430603
10	30.920134517857782	14.335278567061108	36.05708017120455	18.68750674387656
11	27.364133366902855	11.87686580584829	38.726081717800234	22.03291910944862
12	22.55682839981297	13.003452864798762	41.9285688594756	22.51114987591267
13	17.3384167176204	15.808590799554004	44.67912635327123	22.173866129554366
14	17.34394669639967	16.307727583354314	44.4238931050606	21.924432615185413
15	21.417293097867137	14.273010106823007	43.12335899003705	21.18633780527281
16	20.326268747976837	18.668039420206455	40.055974175448696	20.949717656368016
17	20.935016005467038	19.19630975074632	39.58722979534583	20.281444448440816
18	20.37815163831241	25.757921806999246	36.22518253425889	17.63874402042945
19	22.169999640326584	21.95527461065353	33.803231665647594	22.071494083372297
20	20.417760673308635	27.054499514440888	34.106391396611876	18.4213484156386
21	18.682201560982627	20.826394633672628	36.57500989101896	23.91639391432579
22	20.623628745099452	21.935852246160486	33.47327626515124	23.96724274358882
23	21.378628205589326	19.260466496421248	36.892646476998884	22.468258820990542
24	23.619393590619715	17.546532748264575	33.8471118224652	24.986961838650508
25	17.407923605366328	18.42818221055282	37.97288062439305	26.191013559687804
26	16.560757831888644	17.6510178757688	37.49366075603352	28.29456353630903
27	20.619807215048734	20.76560982627774	36.070163291731106	22.544419666942417
28	22.403427687659605	21.567951300219402	32.032064885084345	23.99655612703665
29	22.482106247527245	19.029870877243464	33.636972628853	24.851050246376293
30	26.182696111930365	18.072555119951083	32.48156673740244	23.26318203071611
31	26.01841527892673	20.16594432255512	29.30560551019674	24.510034888321407
32	26.069533863252165	18.82786929468043	30.570262201920656	24.532334640146747
33	21.497275473869728	20.70896126317304	35.69237132683523	22.101391936122
34	21.329937416825523	25.711703772974136	32.82496493184189	20.13339387835845
35	24.953467251735425	27.990234866740998	28.907132323849943	18.149165557673633
36	26.334478293709314	25.62511239794267	29.393006150415424	18.647403157932597
37	23.84063770096752	30.009666223069452	29.288835737150663	16.86086033881236
38	21.94115742905442	26.31833794914218	29.905360932273496	21.835143689529907
39	22.68891846203647	22.187084127612128	34.55360033089954	20.57039707945186
40	18.64281732187174	20.490594540157538	37.54720713592059	23.31938100205014
41	19.616093587022984	23.135947559615868	31.60845951875697	25.63949933460418
42	19.323319425961227	18.156134230119054	35.119411574290545	27.401134769629177
43	22.81210660720066	19.031219652555478	34.28964500233788	23.867028737905983
44	22.116048627845917	19.989434593389202	34.325657303168725	23.56885947559616
45	22.111912383555733	21.566557565730317	33.03960004316081	23.281930007553143
46	22.454726108693308	20.211398050570082	33.12070639859008	24.21316944214653
47	20.407824695176778	19.82191669963673	35.44059993525879	24.329658669927706
48	22.142439664784376	18.16328273927274	32.262345790022664	27.43193180592022
49	21.276750710354996	16.992545768442255	36.40569363018379	25.325009891018958
50	20.396719778441174	16.906269107650253	37.8335970938388	24.863414020069776
51	18.720641657375104	15.897834766032442	36.7641081897637	28.61741538682876
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	495.0
1	3352.0
2	6209.0
3	77194.0
4	148179.0
5	84305.5
6	20432.0
7	17885.5
8	15339.0
9	14776.5
10	14214.0
11	13881.0
12	13548.0
13	12661.5
14	11775.0
15	11119.0
16	10463.0
17	9742.5
18	9022.0
19	8384.5
20	7747.0
21	7241.5
22	6736.0
23	6732.5
24	6729.0
25	6795.5
26	7638.0
27	8414.0
28	8322.5
29	8231.0
30	9286.0
31	10341.0
32	11560.5
33	12780.0
34	15839.0
35	18898.0
36	21045.0
37	23192.0
38	25915.5
39	28639.0
40	35416.0
41	42193.0
42	53103.5
43	64014.0
44	96056.5
45	128099.0
46	170484.0
47	212869.0
48	234369.5
49	255870.0
50	228699.5
51	201529.0
52	173336.5
53	145144.0
54	132180.5
55	119217.0
56	115267.5
57	111318.0
58	108773.5
59	106229.0
60	103383.5
61	100538.0
62	98618.0
63	96698.0
64	91839.0
65	86980.0
66	72447.0
67	57914.0
68	46361.0
69	34808.0
70	28953.0
71	23098.0
72	21128.5
73	19159.0
74	16525.5
75	10846.0
76	7800.0
77	5967.0
78	4134.0
79	3112.0
80	2090.0
81	1785.0
82	1480.0
83	1027.5
84	575.0
85	366.0
86	157.0
87	137.5
88	118.0
89	76.0
90	34.0
91	32.0
92	30.0
93	18.5
94	7.0
95	4.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2224240.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.12043119202843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.93340951623935	21.967857429339187
2	11.070878337667716	6.669192584100771
3	4.0558597401491525	3.664927326830426
4	2.115576304899327	2.5488828209282373
5	1.2920481551768417	1.9458523777395664
6	1.0117627726490561	1.828483858573908
7	0.7542538978809435	1.590291684270954
8	0.6047877532399483	1.4573174325796263
9	0.521075533878522	1.412551778763375
>10	5.1943856544047184	30.905199795086112
>50	0.2621383538945692	5.380146630119617
>100	0.17042528195777298	9.226909251546498
>500	0.007226264069527521	1.481163980783499
>1k	0.005118603715915327	2.8785295585395705
>5k	9.032830086909402E-4	2.0937357483687395
>10k+	1.505471681151567E-4	4.948957742429902
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	109139	4.906799625939646	No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	9912	0.44563536309031404	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	9819	0.4414541596230622	No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT	9350	0.42036830557853466	No Hit
CGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG	6036	0.2713735927777578	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	5544	0.2492536776606841	No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	5512	0.24781498399453297	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC	4385	0.19714599143977268	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCC	3748	0.16850699564795166	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT	3560	0.16005467035931376	No Hit
GGGCCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC	3115	0.14004783656439954	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCT	3051	0.13717044923209726	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT	2623	0.11792792144732582	No Hit
TCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	2406	0.10817178002373845	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTC	2349	0.10560910693090672	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.43457540553177715	0.0
2	0.0	0.0	0.0	1.3249469481710607	0.0
3	0.0	0.0	0.0	2.1673920080566846	0.0
4	0.0	0.0	0.0	2.7658885731755567	0.0
5	0.0	0.0	0.0	5.602452972700788	0.0
6	0.0	0.0	0.0	6.758443333453225	0.0
7	0.0	0.0	0.0	9.899651116785959	0.0
8	0.0	0.0	0.0	13.59799302233572	0.0
9	0.0	0.0	0.0	17.038494047404956	0.0
10	0.0	0.0	0.0	18.983113333093552	0.0
11	0.0	0.0	0.0	20.54364636909686	0.0
12	0.0	0.0	0.0	21.628556270906017	0.0
13	0.0	0.0	0.0	22.204887961730748	0.0
14	0.0	0.0	0.0	22.556603603927634	0.0
15	0.0	0.0	0.0	23.403094989749306	0.0
16	0.0	0.0	0.0	24.2940060425134	0.0
17	4.495917706722296E-5	0.0	0.0	25.398338308815596	0.0
18	4.495917706722296E-5	0.0	0.0	26.0882818400892	8.991835413444592E-5
19	4.495917706722296E-5	0.0	0.0	26.837481566737402	8.991835413444592E-5
20	4.495917706722296E-5	0.0	0.0	27.676060137395247	8.991835413444592E-5
21	4.495917706722296E-5	0.0	0.0	28.51684170772938	8.991835413444592E-5
22	8.991835413444592E-5	0.0	0.0	29.274089127072617	8.991835413444592E-5
23	8.991835413444592E-5	0.0	0.0	29.99222206236737	8.991835413444592E-5
24	1.3487753120166888E-4	0.0	0.0	30.58671726072726	8.991835413444592E-5
25	1.7983670826889184E-4	0.0	0.0	31.152078912347587	8.991835413444592E-5
26	1.7983670826889184E-4	0.0	0.0	31.711146279178507	8.991835413444592E-5
27	1.7983670826889184E-4	0.0	0.0	32.270753156134234	8.991835413444592E-5
28	2.247958853361148E-4	0.0	0.0	32.79425781390498	8.991835413444592E-5
29	2.247958853361148E-4	0.0	0.0	33.368431464230476	8.991835413444592E-5
30	2.247958853361148E-4	0.0	0.0	33.916393914325795	8.991835413444592E-5
31	2.6975506240333775E-4	0.0	0.0	34.46183865050534	8.991835413444592E-5
32	2.6975506240333775E-4	0.0	0.0	35.05345646153293	8.991835413444592E-5
33	2.6975506240333775E-4	0.0	0.0	35.6007894831493	8.991835413444592E-5
34	2.6975506240333775E-4	0.0	0.0	36.210930475128585	8.991835413444592E-5
35	2.6975506240333775E-4	0.0	0.0	36.74787792684243	8.991835413444592E-5
36	2.6975506240333775E-4	0.0	0.0	37.31364421105636	8.991835413444592E-5
37	2.6975506240333775E-4	0.0	0.0	38.09512462683883	8.991835413444592E-5
38	2.6975506240333775E-4	0.0	0.0	38.879662266661875	8.991835413444592E-5
39	2.6975506240333775E-4	0.0	0.0	39.733437039168436	8.991835413444592E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	25	3.891986E-5	45.000004	35
TATCGTG	20	7.0347096E-4	45.000004	11
CGTTTTT	27360	0.0	44.5477	1
TCACGAC	240	0.0	43.125004	24
ATTACGG	170	0.0	41.029415	1
TCGGGTA	55	6.184564E-11	40.909092	4
ACGGGTA	155	0.0	40.64516	4
AATACGG	50	1.0822987E-9	40.500004	1
GTTTTTT	30675	0.0	40.38631	2
CTAGCGG	565	0.0	40.221237	1
CACTTAC	2500	0.0	40.140003	36
GCACTTA	2495	0.0	40.04008	35
CGCACTT	2490	0.0	40.03012	34
TAAACGG	90	0.0	40.000004	1
CGTTCTG	590	0.0	39.66102	1
TATACGG	125	0.0	39.600002	1
CCCGCAC	2550	0.0	39.52941	32
CGGTCTA	245	0.0	39.489796	30
CTTACTG	2520	0.0	39.374996	38
ACCCGCA	2550	0.0	39.352943	31
>>END_MODULE