FastQCFastQC Report
Thu 26 May 2016
SRR1043340_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043340_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1131059
Sequences flagged as poor quality0
Sequence length52
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT933608.254211318772937No Hit
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT70950.6272882316483932RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCTT63600.5623048841837606RNA PCR Primer, Index 16 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC50440.4459537477708944RNA PCR Primer, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT43940.3884854813055729RNA PCR Primer, Index 14 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT34170.30210625617231285RNA PCR Primer, Index 14 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTC32750.2895516502675811No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT26860.23747655957823596No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCG24820.21944036517988894No Hit
CGTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG23830.2106875061336323RNA PCR Primer, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT23050.2037913141577937RNA PCR Primer, Index 14 (95% over 24bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCT18630.16471289296137512No Hit
ACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC16710.14773765117469556RNA PCR Primer, Index 14 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC16540.14623463497483333RNA PCR Primer, Index 14 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC12940.11440605662480913RNA PCR Primer, Index 16 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC12830.11343351673078063RNA PCR Primer, Index 16 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT12020.1062720866020252RNA PCR Primer, Index 14 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT206.3114107E-446.00000417
ACGTTGG551.8189894E-1246.0000041
ATTACGG551.8189894E-1246.0000041
TACGGGT351.01954356E-746.0000043
CGTTCAA405.6115823E-946.0000049
ACTACGG405.6115823E-946.0000041
CCGCGGA206.3114107E-446.0000042
CCGCCTA206.3114107E-446.00000412
CTGTCGG501.6370905E-1146.01
GCGACTC301.8613773E-646.08
CGCGGAA301.8613773E-646.03
CGGATAG253.4171222E-546.05
TATTCGC253.4171222E-546.020
ACGTGCG301.8613773E-646.045
TTATTCG253.4171222E-546.019
CTTACGG501.6370905E-1146.01
CGTTTTT176500.045.6481551
GCTTAGG2000.044.851
CGTTTTC3100.044.516131
GCTACGG1450.044.4137951
Produced by FastQC (version 0.11.5)