##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043340_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1131059
Sequences flagged as poor quality	0
Sequence length	52
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23949590604911	33.0	31.0	34.0	31.0	34.0
2	32.57950734665477	34.0	31.0	34.0	31.0	34.0
3	32.63423305061893	34.0	31.0	34.0	31.0	34.0
4	36.17555494452544	37.0	35.0	37.0	35.0	37.0
5	36.0730766476373	37.0	35.0	37.0	35.0	37.0
6	36.30696099849787	37.0	37.0	37.0	35.0	37.0
7	36.239147559941614	37.0	37.0	37.0	35.0	37.0
8	36.217139866266926	37.0	37.0	37.0	35.0	37.0
9	37.74509994615666	39.0	38.0	39.0	35.0	39.0
10	37.59330680362386	39.0	37.0	39.0	35.0	39.0
11	37.34235879825898	39.0	37.0	39.0	35.0	39.0
12	37.36483773171868	39.0	37.0	39.0	34.0	39.0
13	37.378260550510625	39.0	37.0	39.0	34.0	39.0
14	38.806829705612174	41.0	38.0	41.0	35.0	41.0
15	38.89441311195968	41.0	38.0	41.0	35.0	41.0
16	38.91310709697726	41.0	38.0	41.0	35.0	41.0
17	38.880505791475066	41.0	38.0	41.0	35.0	41.0
18	38.55879578342067	40.0	38.0	41.0	35.0	41.0
19	38.21645997246828	40.0	37.0	41.0	35.0	41.0
20	37.867696556943535	40.0	35.0	41.0	34.0	41.0
21	37.79245379772408	40.0	35.0	41.0	34.0	41.0
22	37.73905782103321	40.0	35.0	41.0	34.0	41.0
23	37.71799967994596	39.0	35.0	41.0	34.0	41.0
24	37.63042688312458	39.0	35.0	41.0	34.0	41.0
25	37.54766904290581	39.0	35.0	41.0	34.0	41.0
26	37.48818054584244	39.0	35.0	41.0	34.0	41.0
27	37.521128429197766	39.0	35.0	41.0	34.0	41.0
28	37.49703773189551	39.0	35.0	41.0	34.0	41.0
29	37.430193296724575	39.0	35.0	41.0	33.0	41.0
30	37.390595892875616	40.0	35.0	41.0	33.0	41.0
31	37.24712150294547	40.0	35.0	41.0	33.0	41.0
32	36.99267942697949	40.0	35.0	41.0	33.0	41.0
33	36.727828521765886	40.0	35.0	41.0	32.0	41.0
34	36.46646461413596	40.0	35.0	41.0	31.0	41.0
35	36.26987097932115	39.0	35.0	41.0	30.0	41.0
36	36.18013914393502	39.0	35.0	41.0	30.0	41.0
37	36.12046232778308	39.0	35.0	41.0	30.0	41.0
38	36.06871701653053	39.0	35.0	41.0	30.0	41.0
39	36.04031001035313	39.0	35.0	41.0	30.0	41.0
40	35.98855055306576	39.0	35.0	41.0	30.0	41.0
41	35.96662508321847	39.0	35.0	41.0	30.0	41.0
42	35.899602054357906	39.0	35.0	41.0	30.0	41.0
43	35.79560571110791	39.0	35.0	41.0	29.0	41.0
44	35.62943047179679	39.0	35.0	41.0	28.0	41.0
45	35.56684841374322	38.0	35.0	41.0	27.0	41.0
46	35.55194910256671	38.0	35.0	41.0	27.0	41.0
47	35.47570993201946	38.0	35.0	41.0	27.0	41.0
48	35.396518660830246	38.0	35.0	40.0	27.0	41.0
49	35.32354810845411	38.0	35.0	40.0	26.0	41.0
50	35.211030547478074	38.0	35.0	40.0	26.0	41.0
51	35.13248645738197	38.0	35.0	40.0	26.0	41.0
52	34.814095462747744	37.0	35.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	2.0
14	5.0
15	15.0
16	45.0
17	86.0
18	213.0
19	425.0
20	843.0
21	1343.0
22	2182.0
23	3791.0
24	7169.0
25	12939.0
26	18161.0
27	18841.0
28	16634.0
29	15850.0
30	17358.0
31	20346.0
32	25280.0
33	33067.0
34	57194.0
35	99577.0
36	129176.0
37	85687.0
38	140212.0
39	423640.0
40	976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.458530456855037	18.604511347330245	24.540010733303923	31.3969474625108
2	36.04312418715558	18.933937133252996	24.925313356774492	20.09762532281694
3	20.083214049841786	20.631284486485672	40.32884226198633	18.956659201686207
4	21.727867423361648	23.557303376746923	37.63269643758637	17.08213276230506
5	19.704807618347054	28.459788569827037	33.50391093656476	18.331492875261148
6	70.28050702925312	1.6369614670852715	23.95312711361653	4.129404390045082
7	70.61700583258698	1.6444765480845829	20.889803272861982	6.848714346466453
8	59.88396714937063	3.5529534710390878	28.03337403265435	8.529705346935925
9	31.676950539273367	18.86338378457711	31.802585011038325	17.65708066511119
10	23.773030407785978	19.722401749157207	43.29128719191483	13.213280651141984
11	21.80124997900198	16.072282701432904	43.141869699104994	18.984597620460118
12	17.706149723400813	19.831679868158954	44.21210564612456	18.25006476231567
13	13.391697515337398	22.283629766440125	43.51373358949445	20.81093912872803
14	12.202192812222881	21.70549900579899	45.13115584598151	20.96115233599662
15	12.653362910334472	19.162926071937893	47.00709688884488	21.176614128882754
16	13.48621071049344	24.445762776300796	40.89203127334648	21.175995239859283
17	14.712053040557565	22.228283405198138	42.644194511515316	20.415469042728983
18	16.24627892974637	28.375089186328918	39.337735697253635	16.040896186671073
19	16.380489435122307	23.771350566150836	37.898465066809074	21.949694931917787
20	13.474805469918014	28.542277635384185	36.69030528027273	21.292611614425066
21	14.194042927910921	24.08291698311052	37.63101659595123	24.09202349302733
22	12.465485885351693	26.134180445051935	35.14069557821475	26.259638091381614
23	12.01184023114621	23.016217544796515	44.56230842069247	20.40963380336481
24	16.44450024269291	22.52358188211225	40.025763465919994	21.006154409274846
25	14.08759401587362	25.5108707856973	39.374780625944354	21.026754572484723
26	14.566260469170928	23.21868266818972	38.11286590708354	24.10219095555581
27	18.66339421727779	24.04878967410188	38.168919570066635	19.11889653855369
28	14.546279194984525	23.461640816261575	35.38223912280438	26.60984086594952
29	15.174893617397501	26.892673149676543	33.88311308251824	24.049320150407716
30	17.24914438592505	27.30016736527449	31.838480574399743	23.612207674400718
31	15.471606697793838	25.972738822643205	31.433815565766242	27.121838913796715
32	13.06881427051993	29.68377423282075	31.52435018862853	25.723061308030793
33	12.57396828989469	26.734414385102813	32.31705861497941	28.374558710023084
34	13.41857498149964	27.283103710770174	31.981001875233737	27.31731943249645
35	14.231441507472201	24.0826517449576	34.164884413633594	27.5210223339366
36	16.885237640123105	26.158051878814458	31.26450521148764	25.692205269574796
37	14.20093911988676	25.437046166468768	38.11313114523646	22.248883568408015
38	16.22435257577191	24.32835068727626	35.443597548845815	24.003699188106015
39	21.79603362866128	21.516207377333984	36.79516276339253	19.8925962306122
40	16.768532852839684	21.497817532065085	39.559828443962694	22.17382117113254
41	17.968470256635598	24.766258877742008	33.63069477365902	23.63457609196337
42	18.290734612429592	20.464449688300963	37.747809795952286	23.497005903317156
43	20.452248733266785	21.913357304968176	35.00754602545049	22.62684793631455
44	15.002400405283897	25.421308702729036	35.24413845785233	24.332152434134734
45	16.70363791809269	23.10896248559978	35.7091009399156	24.47829865639193
46	20.19284581971409	21.835288875292978	34.609600383357545	23.362264921635386
47	15.726854213617504	19.700917458770938	38.74015413873193	25.832074188879627
48	17.48432221484467	17.903928972759157	34.54877243362194	30.06297637877423
49	17.829485464507155	18.531482442560467	38.94491799278376	24.694114100148624
50	16.716015698562146	17.26426296064131	40.19374762943401	25.825973711362536
51	15.016988503694325	17.12448245405412	39.00892880035436	28.849600241897193
52	13.675855989829	17.74540497003251	45.01206391532184	23.566675124816655
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	426.0
1	1617.0
2	2808.0
3	52323.0
4	101838.0
5	55408.5
6	8979.0
7	8500.0
8	8021.0
9	8713.0
10	9405.0
11	9691.5
12	9978.0
13	9863.5
14	9128.5
15	8508.0
16	7674.0
17	6840.0
18	6212.0
19	5584.0
20	5270.5
21	4957.0
22	4858.0
23	4759.0
24	4973.5
25	5188.0
26	5615.0
27	6042.0
28	6410.5
29	6779.0
30	7147.5
31	7516.0
32	9424.5
33	11333.0
34	13671.0
35	16009.0
36	18945.5
37	21882.0
38	23356.5
39	26577.0
40	28323.0
41	34039.5
42	39756.0
43	50277.0
44	60798.0
45	81241.5
46	101685.0
47	125636.0
48	149587.0
49	153328.0
50	157069.0
51	137566.0
52	118063.0
53	93938.0
54	69813.0
55	55466.5
56	41120.0
57	33840.5
58	26561.0
59	22877.0
60	19193.0
61	15974.0
62	12755.0
63	10796.0
64	6841.0
65	4845.0
66	3971.5
67	3098.0
68	2567.0
69	2036.0
70	1893.5
71	1751.0
72	1824.5
73	1898.0
74	1571.5
75	1245.0
76	932.0
77	619.0
78	480.0
79	341.0
80	235.0
81	129.0
82	95.0
83	61.0
84	43.0
85	25.0
86	18.0
87	11.0
88	8.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1131059.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.17549594178994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6298870715902	25.090590116215722
2	11.165933826953374	7.408707847247734
3	4.175042063920643	4.155272731452048
4	2.054169751689254	2.7259240104365796
5	1.1772074137029325	1.9527219887973335
6	0.7798471700539187	1.5523089975204087
7	0.5459005392084314	1.267736478619118
8	0.4379555855940776	1.1623515002048448
9	0.3273789015184033	0.9774861676876294
>10	3.2192273446929134	22.906233441075088
>50	0.32211387404102754	7.219359263155502
>100	0.1532973946303479	9.163521388787608
>500	0.00642083328241492	1.452580208454582
>1k	0.004548090241710568	3.037051788641017
>5k	8.02604160301865E-4	1.6418966929122152
>10k+	2.675347201006217E-4	8.286257378792605
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93360	8.254211318772937	No Hit
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT	7095	0.6272882316483932	RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCTT	6360	0.5623048841837606	RNA PCR Primer, Index 16 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	5044	0.4459537477708944	RNA PCR Primer, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT	4394	0.3884854813055729	RNA PCR Primer, Index 14 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT	3417	0.30210625617231285	RNA PCR Primer, Index 14 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTC	3275	0.2895516502675811	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT	2686	0.23747655957823596	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCG	2482	0.21944036517988894	No Hit
CGTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG	2383	0.2106875061336323	RNA PCR Primer, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT	2305	0.2037913141577937	RNA PCR Primer, Index 14 (95% over 24bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCT	1863	0.16471289296137512	No Hit
ACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	1671	0.14773765117469556	RNA PCR Primer, Index 14 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	1654	0.14623463497483333	RNA PCR Primer, Index 14 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	1294	0.11440605662480913	RNA PCR Primer, Index 16 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	1283	0.11343351673078063	RNA PCR Primer, Index 16 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT	1202	0.1062720866020252	RNA PCR Primer, Index 14 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5771582207471051	0.0
2	0.0	0.0	0.0	1.9388908978223063	0.0
3	0.0	0.0	0.0	3.3785151791374277	0.0
4	0.0	0.0	0.0	4.318077129486614	0.0
5	0.0	0.0	0.0	8.720323166165514	0.0
6	0.0	0.0	0.0	10.345172090934248	0.0
7	0.0	0.0	0.0	14.325777877192968	0.0
8	0.0	0.0	0.0	18.953564756568845	0.0
9	0.0	0.0	0.0	22.5107620380546	0.0
10	0.0	0.0	0.0	24.57484534405367	0.0
11	0.0	0.0	0.0	26.384123197817267	0.0
12	0.0	0.0	0.0	27.65947664975921	0.0
13	0.0	0.0	0.0	28.214708516531854	0.0
14	0.0	0.0	0.0	28.63157448019953	0.0
15	0.0	0.0	0.0	29.834252678242247	0.0
16	0.0	0.0	0.0	31.25460298711208	0.0
17	0.0	0.0	0.0	32.797316497194224	0.0
18	0.0	0.0	0.0	33.616725564272066	0.0
19	0.0	0.0	0.0	34.510136076013715	0.0
20	0.0	0.0	0.0	35.53377852083755	0.0
21	0.0	0.0	0.0	36.50985492357162	0.0
22	8.841271763895605E-5	0.0	0.0	37.54896959398228	0.0
23	1.768254352779121E-4	0.0	0.0	38.39560977809292	0.0
24	3.536508705558242E-4	0.0	0.0	39.071790242595654	0.0
25	3.536508705558242E-4	0.0	0.0	39.69421577477391	0.0
26	3.536508705558242E-4	0.0	0.0	40.348027821713984	0.0
27	3.536508705558242E-4	0.0	0.0	40.98318478523225	0.0
28	3.536508705558242E-4	0.0	0.0	41.63089635465524	0.0
29	3.536508705558242E-4	0.0	0.0	42.39469382233818	0.0
30	3.536508705558242E-4	0.0	0.0	43.01561633831657	0.0
31	3.536508705558242E-4	0.0	0.0	43.556878995702256	0.0
32	3.536508705558242E-4	0.0	0.0	44.10079403461712	0.0
33	3.536508705558242E-4	0.0	0.0	44.67202860328241	0.0
34	3.536508705558242E-4	0.0	0.0	45.31408175877651	0.0
35	3.536508705558242E-4	0.0	0.0	45.83562838012871	0.0
36	3.536508705558242E-4	0.0	0.0	46.332419440542004	0.0
37	3.536508705558242E-4	0.0	0.0	46.86820050943408	0.0
38	3.536508705558242E-4	0.0	0.0	47.376485223140435	0.0
39	3.536508705558242E-4	0.0	0.0	47.87186168007151	0.0
40	4.420635881947803E-4	0.0	0.0	48.38173782269537	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAT	20	6.3114107E-4	46.000004	17
ACGTTGG	55	1.8189894E-12	46.000004	1
ATTACGG	55	1.8189894E-12	46.000004	1
TACGGGT	35	1.01954356E-7	46.000004	3
CGTTCAA	40	5.6115823E-9	46.000004	9
ACTACGG	40	5.6115823E-9	46.000004	1
CCGCGGA	20	6.3114107E-4	46.000004	2
CCGCCTA	20	6.3114107E-4	46.000004	12
CTGTCGG	50	1.6370905E-11	46.0	1
GCGACTC	30	1.8613773E-6	46.0	8
CGCGGAA	30	1.8613773E-6	46.0	3
CGGATAG	25	3.4171222E-5	46.0	5
TATTCGC	25	3.4171222E-5	46.0	20
ACGTGCG	30	1.8613773E-6	46.0	45
TTATTCG	25	3.4171222E-5	46.0	19
CTTACGG	50	1.6370905E-11	46.0	1
CGTTTTT	17650	0.0	45.648155	1
GCTTAGG	200	0.0	44.85	1
CGTTTTC	310	0.0	44.51613	1
GCTACGG	145	0.0	44.413795	1
>>END_MODULE