SRR1043339_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043339_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1100855
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	85735	7.788037479958759	No Hit
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	6656	0.6046209537132502	RNA PCR Primer, Index 16 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT	6157	0.5592925498816829	RNA PCR Primer, Index 16 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG	4891	0.4442910283370653	RNA PCR Primer, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	4609	0.4186745756707287	RNA PCR Primer, Index 14 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTC	3260	0.29613345990162193	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT	3064	0.2783291169136717	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCT	2620	0.23799682973688632	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCC	2381	0.21628643190974287	No Hit
CGTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT	2304	0.20929186859304813	RNA PCR Primer, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	2148	0.19512106499039383	RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTC	1730	0.1571505784140509	No Hit
ACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG	1700	0.15442542387507893	RNA PCR Primer, Index 14 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG	1652	0.15006517661272373	RNA PCR Primer, Index 14 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG	1310	0.11899841486844316	RNA PCR Primer, Index 16 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG	1204	0.10936953549740883	RNA PCR Primer, Index 16 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	1151	0.10455509581189165	RNA PCR Primer, Index 14 (95% over 23bp)
TGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCTT	1132	0.10282916460387609	RNA PCR Primer, Index 14 (96% over 25bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1106	0.10046736400343369	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGC	30	2.1654178E-6	45.000004	7
CTCCGTT	30	2.1654178E-6	45.000004	21
GCTCCGT	30	2.1654178E-6	45.000004	20
AACGTGC	25	3.890699E-5	45.0	44
CGAACGT	25	3.890699E-5	45.0	42
ATTACGG	35	1.2118653E-7	45.0	1
GTCTCCG	20	7.0331566E-4	45.0	8
TACGCGG	100	0.0	45.0	1
CGCTAGC	20	7.0331566E-4	45.0	10
ATCCCGG	20	7.0331566E-4	45.0	1
TGTTCGG	70	0.0	45.0	1
ACGTGCG	25	3.890699E-5	45.0	45
CGCAGAA	20	7.0331566E-4	45.0	2
GCGATCG	20	7.0331566E-4	45.0	8
TCGTAGC	20	7.0331566E-4	45.0	1
CATCGAA	25	3.890699E-5	45.0	13
ACATCGA	25	3.890699E-5	45.0	12
CTCTATC	25	3.890699E-5	45.0	20
TCAACGA	20	7.0331566E-4	45.0	20
CGAAGGA	25	3.890699E-5	45.0	16

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content