FastQCFastQC Report
Thu 26 May 2016
SRR1043339_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043339_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1100855
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT857357.788037479958759No Hit
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC66560.6046209537132502RNA PCR Primer, Index 16 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT61570.5592925498816829RNA PCR Primer, Index 16 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG48910.4442910283370653RNA PCR Primer, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC46090.4186745756707287RNA PCR Primer, Index 14 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTC32600.29613345990162193No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT30640.2783291169136717No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCT26200.23799682973688632No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCC23810.21628643190974287No Hit
CGTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT23040.20929186859304813RNA PCR Primer, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC21480.19512106499039383RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTC17300.1571505784140509No Hit
ACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG17000.15442542387507893RNA PCR Primer, Index 14 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG16520.15006517661272373RNA PCR Primer, Index 14 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG13100.11899841486844316RNA PCR Primer, Index 16 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG12040.10936953549740883RNA PCR Primer, Index 16 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC11510.10455509581189165RNA PCR Primer, Index 14 (95% over 23bp)
TGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCTT11320.10282916460387609RNA PCR Primer, Index 14 (96% over 25bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC11060.10046736400343369No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC302.1654178E-645.0000047
CTCCGTT302.1654178E-645.00000421
GCTCCGT302.1654178E-645.00000420
AACGTGC253.890699E-545.044
CGAACGT253.890699E-545.042
ATTACGG351.2118653E-745.01
GTCTCCG207.0331566E-445.08
TACGCGG1000.045.01
CGCTAGC207.0331566E-445.010
ATCCCGG207.0331566E-445.01
TGTTCGG700.045.01
ACGTGCG253.890699E-545.045
CGCAGAA207.0331566E-445.02
GCGATCG207.0331566E-445.08
TCGTAGC207.0331566E-445.01
CATCGAA253.890699E-545.013
ACATCGA253.890699E-545.012
CTCTATC253.890699E-545.020
TCAACGA207.0331566E-445.020
CGAAGGA253.890699E-545.016