##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043339_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1100855
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18123912776887	33.0	31.0	34.0	30.0	34.0
2	32.4958545857538	34.0	31.0	34.0	31.0	34.0
3	32.5356772690318	34.0	31.0	34.0	31.0	34.0
4	36.12423343673781	37.0	35.0	37.0	35.0	37.0
5	36.08343969005909	37.0	35.0	37.0	35.0	37.0
6	36.30843662426023	37.0	37.0	37.0	35.0	37.0
7	36.251301942580994	37.0	37.0	37.0	35.0	37.0
8	36.15267769143075	37.0	37.0	37.0	35.0	37.0
9	37.6910228867562	39.0	38.0	39.0	35.0	39.0
10	37.528802612514816	39.0	37.0	39.0	35.0	39.0
11	37.32871449918473	39.0	37.0	39.0	34.0	39.0
12	37.36101121401093	39.0	37.0	39.0	34.0	39.0
13	37.380356177698246	39.0	37.0	39.0	34.0	39.0
14	38.804948880642776	40.0	38.0	41.0	35.0	41.0
15	38.90122132342588	41.0	38.0	41.0	35.0	41.0
16	38.94748627203401	41.0	38.0	41.0	35.0	41.0
17	38.893080378433126	41.0	38.0	41.0	35.0	41.0
18	38.60685376366551	40.0	38.0	41.0	35.0	41.0
19	38.29431850697867	40.0	37.0	41.0	35.0	41.0
20	37.97370498385346	40.0	35.0	41.0	34.0	41.0
21	37.89853886297469	40.0	35.0	41.0	34.0	41.0
22	37.855960140072945	40.0	35.0	41.0	34.0	41.0
23	37.80885856902135	40.0	35.0	41.0	34.0	41.0
24	37.72839111417943	39.0	35.0	41.0	34.0	41.0
25	37.711634138919294	39.0	35.0	41.0	34.0	41.0
26	37.676588651548116	39.0	35.0	41.0	34.0	41.0
27	37.680159512379014	39.0	35.0	41.0	34.0	41.0
28	37.598670124584984	39.0	35.0	41.0	34.0	41.0
29	37.57799801063719	40.0	35.0	41.0	34.0	41.0
30	37.48488129681021	40.0	35.0	41.0	34.0	41.0
31	37.32210054911864	40.0	35.0	41.0	33.0	41.0
32	37.08285287344837	40.0	35.0	41.0	33.0	41.0
33	36.78390432890799	40.0	35.0	41.0	32.0	41.0
34	36.49291596077594	39.0	35.0	41.0	31.0	41.0
35	36.2965349660037	39.0	35.0	41.0	31.0	41.0
36	36.202002988586145	39.0	35.0	41.0	30.0	41.0
37	36.106224707159434	39.0	35.0	41.0	30.0	41.0
38	36.062525037357325	39.0	35.0	41.0	30.0	41.0
39	35.98666581884081	39.0	35.0	41.0	30.0	41.0
40	35.91121537350514	39.0	35.0	41.0	30.0	41.0
41	35.84207729446658	39.0	35.0	41.0	29.0	41.0
42	35.7639462054494	39.0	35.0	41.0	29.0	41.0
43	35.63635992024381	38.0	35.0	40.0	28.0	41.0
44	35.556371184215905	38.0	35.0	40.0	28.0	41.0
45	35.509971794650525	38.0	35.0	40.0	27.0	41.0
46	35.429583369290235	38.0	35.0	40.0	27.0	41.0
47	35.29094930758365	38.0	35.0	40.0	26.0	41.0
48	35.24233255060839	38.0	35.0	40.0	26.0	41.0
49	35.15059658174782	38.0	35.0	40.0	26.0	41.0
50	35.06748663538795	38.0	35.0	40.0	26.0	41.0
51	34.6594910319706	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	6.0
15	11.0
16	24.0
17	61.0
18	135.0
19	369.0
20	745.0
21	1232.0
22	1844.0
23	3088.0
24	5704.0
25	10767.0
26	16698.0
27	18774.0
28	17381.0
29	16426.0
30	17677.0
31	19835.0
32	24392.0
33	31946.0
34	56748.0
35	97677.0
36	120040.0
37	85498.0
38	148129.0
39	405557.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.958005368554442	18.650140118362547	24.749217653551103	30.64263685953191
2	35.54691580635052	19.12513455450536	24.969682655753935	20.358266983390184
3	20.346639657357237	20.740333649754056	39.67661499470866	19.23641169818005
4	21.96901499289189	23.797139496118927	37.04947518065504	17.18437033033415
5	19.939955761657984	28.68806518569657	32.879080351181585	18.49289870146386
6	70.98936735537377	1.600664937707509	23.26200998314946	4.147957723769252
7	71.30784708249497	1.5844048489583096	20.193940164690172	6.913807903856547
8	60.313301933497144	3.5105440771037055	27.461654804674545	8.7144991847246
9	31.94771336824559	18.860976241194344	31.257613400493252	17.93369699006681
10	24.115619223240117	19.683427881056087	42.896475920988685	13.304476974715108
11	22.088830954122024	16.03926039305812	42.711165412338595	19.160743240481263
12	17.883554146549727	19.76836186418738	43.88670624196647	18.46137774729642
13	13.500506424551824	22.16604366605956	43.20378251450009	21.129667394888518
14	12.30852382920548	21.668248770274015	44.79064000254348	21.232587397977028
15	12.77834047172425	19.046195911359806	46.713418206757474	21.462045410158467
16	13.58471369980606	24.39058731622239	40.616157441261564	21.408541542709987
17	14.95028863928492	22.145877522471167	42.34508631927002	20.558747518973888
18	16.447851896934658	28.38666309368627	38.99977744571265	16.165707563666423
19	16.529969887042345	23.74000208928515	37.49894400261615	22.23108402105636
20	13.585349569198488	28.610670796789766	36.26581157373133	21.53816806028042
21	14.243746905814117	24.042766758564934	37.14767158254266	24.56581475307829
22	12.568049379800247	26.10098514336584	34.62681279550894	26.70415268132497
23	12.14228940232819	22.992310522275865	44.21417897906627	20.65122109632967
24	16.54114302065213	22.532122759128132	39.60621516911855	21.32051905110119
25	14.047263263554239	25.475380499702503	39.19017490950216	21.2871813272411
26	14.745720371892757	23.08505661508555	37.74584300384701	24.423380009174686
27	18.92665246558357	23.935395669729438	37.89908752742186	19.238864337265127
28	14.793864768747927	23.48874284079193	34.79222967602455	26.925162714435597
29	15.348797071367256	26.945873888931786	33.361069350641095	24.344259689059868
30	17.512478936826376	27.188685158354186	31.2726017504576	24.026234154361838
31	15.736404885293704	25.926211898933104	30.82776569121274	27.509617524560454
32	13.1723978180596	29.656403431877948	30.953304476974715	26.217894273087737
33	12.63663243569771	26.835050937680254	31.70735473790826	28.820961888713768
34	13.578536682851055	27.336661049820364	31.43965372369658	27.645148543631997
35	14.368649822183666	23.940664301838115	33.694174073788105	27.996511802190117
36	17.06046663729556	25.898687838089486	30.737653914457397	26.303191610157562
37	14.361473581897707	25.318048244319186	37.724132606019865	22.596345567763237
38	16.496632163182255	24.103265189330113	35.093540929550215	24.306561717937424
39	22.119897715866305	21.31188939506111	36.50380840346821	20.06440448560437
40	17.030853291305394	21.219688333159226	39.34678045700842	22.402677918526965
41	18.231738058145712	24.568721584586527	33.26196456390714	23.937575793360615
42	18.474731004537382	20.21456050070173	37.45007289788392	23.86063559687697
43	20.719168282834705	21.720480898937645	34.62218003279269	22.93817078543496
44	15.197823509908206	25.218398426677446	35.073465624446456	24.510312438967894
45	16.88905441679422	22.86813431378338	35.48305635165394	24.75975491776846
46	20.545303423248292	21.59984739134582	34.317326078366364	23.53752310703953
47	16.074778240549392	19.3868402287313	38.44302837340067	26.09535315731863
48	17.69633602972235	17.585785593924722	34.13019880002362	30.587679576329307
49	18.102656571483074	18.200398780947534	38.62116264176481	25.07578200580458
50	17.016591649218107	16.834823841468676	40.09429034704843	26.054294162264785
51	15.271947713368245	16.90540534402805	38.59945224393766	29.22319469866604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	257.0
1	1394.0
2	2531.0
3	48190.5
4	93850.0
5	51594.5
6	9339.0
7	8570.5
8	7802.0
9	8277.5
10	8753.0
11	8920.5
12	9088.0
13	8968.0
14	8848.0
15	8169.5
16	7491.0
17	6815.0
18	6139.0
19	5546.0
20	4953.0
21	4794.5
22	4636.0
23	4490.5
24	4345.0
25	4480.5
26	5040.0
27	5464.0
28	6023.5
29	6583.0
30	7413.0
31	8243.0
32	9913.5
33	11584.0
34	14538.5
35	17493.0
36	19967.5
37	22442.0
38	24376.5
39	26311.0
40	29609.5
41	32908.0
42	39963.5
43	47019.0
44	57013.5
45	67008.0
46	90815.5
47	114623.0
48	138625.0
49	162627.0
50	160144.5
51	157662.0
52	126461.5
53	95261.0
54	74368.5
55	53476.0
56	42781.0
57	32086.0
58	27770.5
59	23455.0
60	19252.5
61	15050.0
62	12480.0
63	9910.0
64	8085.0
65	6260.0
66	5130.0
67	4000.0
68	3157.0
69	2314.0
70	2099.5
71	1885.0
72	1819.0
73	1753.0
74	1491.0
75	1025.0
76	821.0
77	618.5
78	416.0
79	297.5
80	179.0
81	128.5
82	78.0
83	57.5
84	37.0
85	26.5
86	16.0
87	10.5
88	5.0
89	4.5
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1100855.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.85787821600571
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.99440713088953	24.641570963883183
2	11.482067808286452	7.545527714251915
3	4.279082725604809	4.218047372224097
4	2.036687967688894	2.676849808253034
5	1.191338921753005	1.9572434602473894
6	0.7786459856279108	1.5350792981486172
7	0.5953281402829762	1.3692853672385437
8	0.4301691496971384	1.1307556426425043
9	0.35920585660307014	1.062246806166572
>10	3.3676787517600544	23.61561683437276
>50	0.3210858022575087	7.187580302029134
>100	0.15149179185543157	9.029216302727166
>500	0.007240416522284731	1.6023798201283252
>1k	0.004734118495340016	3.411279241331892
>5k	5.569551170988255E-4	1.1724127818241339
>10k+	2.7847755854941273E-4	7.84490828453072
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	85735	7.788037479958759	No Hit
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	6656	0.6046209537132502	RNA PCR Primer, Index 16 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT	6157	0.5592925498816829	RNA PCR Primer, Index 16 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG	4891	0.4442910283370653	RNA PCR Primer, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	4609	0.4186745756707287	RNA PCR Primer, Index 14 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTC	3260	0.29613345990162193	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT	3064	0.2783291169136717	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCT	2620	0.23799682973688632	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCC	2381	0.21628643190974287	No Hit
CGTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT	2304	0.20929186859304813	RNA PCR Primer, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	2148	0.19512106499039383	RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTC	1730	0.1571505784140509	No Hit
ACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG	1700	0.15442542387507893	RNA PCR Primer, Index 14 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG	1652	0.15006517661272373	RNA PCR Primer, Index 14 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG	1310	0.11899841486844316	RNA PCR Primer, Index 16 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG	1204	0.10936953549740883	RNA PCR Primer, Index 16 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	1151	0.10455509581189165	RNA PCR Primer, Index 14 (95% over 23bp)
TGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCTT	1132	0.10282916460387609	RNA PCR Primer, Index 14 (96% over 25bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1106	0.10046736400343369	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.57319083803044	0.0
2	0.0	0.0	0.0	1.9327704375235613	0.0
3	0.0	0.0	0.0	3.38273432922592	0.0
4	0.0	0.0	0.0	4.3280904387953	0.0
5	0.0	0.0	0.0	8.79025848090802	0.0
6	0.0	0.0	0.0	10.41626735582797	0.0
7	0.0	0.0	0.0	14.477110972834751	0.0
8	0.0	0.0	0.0	19.25376184874484	0.0
9	0.0	0.0	0.0	22.98413505865895	0.0
10	0.0	0.0	0.0	25.14209409958623	0.0
11	0.0	0.0	0.0	26.977394843099226	0.0
12	0.0	0.0	0.0	28.284651475444086	0.0
13	0.0	0.0	0.0	28.866471969514606	0.0
14	0.0	0.0	0.0	29.296228840310487	0.0
15	0.0	0.0	0.0	30.56460660123268	0.0
16	0.0	0.0	0.0	32.03591753682365	0.0
17	0.0	0.0	0.0	33.633675643022926	0.0
18	0.0	0.0	0.0	34.48728488311358	0.0
19	0.0	0.0	0.0	35.42101366665001	0.0
20	0.0	0.0	0.0	36.48981927683482	0.0
21	0.0	0.0	0.0	37.481866367505255	0.0
22	9.083848463239936E-5	0.0	0.0	38.57528920702545	0.0
23	9.083848463239936E-5	0.0	0.0	39.43725558770229	0.0
24	9.083848463239936E-5	0.0	0.0	40.12136021546888	0.0
25	1.816769692647987E-4	0.0	0.0	40.75977308546539	0.0
26	1.816769692647987E-4	0.0	0.0	41.43461218779948	0.0
27	1.816769692647987E-4	0.0	0.0	42.07456931203474	0.0
28	2.7251545389719807E-4	0.0	0.0	42.74450313619868	0.0
29	2.7251545389719807E-4	0.0	0.0	43.51581270921238	0.0
30	2.7251545389719807E-4	0.0	0.0	44.147049338922926	0.0
31	2.7251545389719807E-4	0.0	0.0	44.704979311535126	0.0
32	2.7251545389719807E-4	0.0	0.0	45.25800400597717	0.0
33	2.7251545389719807E-4	0.0	0.0	45.84963505638799	0.0
34	2.7251545389719807E-4	0.0	0.0	46.50839574694215	0.0
35	2.7251545389719807E-4	0.0	0.0	47.04879389202029	0.0
36	2.7251545389719807E-4	0.0	0.0	47.55966952959291	0.0
37	2.7251545389719807E-4	0.0	0.0	48.103156183148556	0.0
38	2.7251545389719807E-4	0.0	0.0	48.621208061007124	0.0
39	2.7251545389719807E-4	0.0	0.0	49.117367864069294	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGC	30	2.1654178E-6	45.000004	7
CTCCGTT	30	2.1654178E-6	45.000004	21
GCTCCGT	30	2.1654178E-6	45.000004	20
AACGTGC	25	3.890699E-5	45.0	44
CGAACGT	25	3.890699E-5	45.0	42
ATTACGG	35	1.2118653E-7	45.0	1
GTCTCCG	20	7.0331566E-4	45.0	8
TACGCGG	100	0.0	45.0	1
CGCTAGC	20	7.0331566E-4	45.0	10
ATCCCGG	20	7.0331566E-4	45.0	1
TGTTCGG	70	0.0	45.0	1
ACGTGCG	25	3.890699E-5	45.0	45
CGCAGAA	20	7.0331566E-4	45.0	2
GCGATCG	20	7.0331566E-4	45.0	8
TCGTAGC	20	7.0331566E-4	45.0	1
CATCGAA	25	3.890699E-5	45.0	13
ACATCGA	25	3.890699E-5	45.0	12
CTCTATC	25	3.890699E-5	45.0	20
TCAACGA	20	7.0331566E-4	45.0	20
CGAAGGA	25	3.890699E-5	45.0	16
>>END_MODULE