FastQCFastQC Report
Thu 26 May 2016
SRR1043338_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043338_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3048626
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1148613.767631713434183No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC238080.7809419718915996No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA122160.40070510452905667No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT107710.35330670275724213Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT89920.2949525458354026Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT63690.2089137860793682Illumina Single End Adapter 2 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC61570.2019598337086937No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC59310.19454665806825763No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC50060.16420512060187115No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC41930.13753736929357685No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC39170.12848411054684963No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA38640.126745622454181No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCG36450.1195620584486257No Hit
GGGCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT31770.10421088057374042No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT30690.10066830106415152No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG206.3131034E-446.01
GTAACGA453.110472E-1046.08
CGTTTTT283400.045.3263971
ACTTACT63350.042.36937737
CACTTAC64150.042.34294536
TCACGAC6100.042.22950724
CGCACTT64750.041.986134
TATACGG1700.041.9411771
CCGCACT64700.041.91190333
GCACTTA65500.041.5053435
ACCCGCA66300.041.42081531
CCCGCAC66650.041.37584732
CTTACTG65500.040.83816538
ATCAACG76050.040.79815714
TCAACGC76450.040.6147815
TTATGAC74000.040.59189226
AACGCAA75550.040.55063217
GGACTTA82550.040.5112087
CTTAATC82000.040.4743910
CTTATGA73850.040.4563325