##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043338_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3048626
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.323123597318926	33.0	31.0	34.0	31.0	34.0
2	32.662161905068054	34.0	31.0	34.0	31.0	34.0
3	32.79953789018397	34.0	31.0	34.0	31.0	34.0
4	36.22465727183328	37.0	37.0	37.0	35.0	37.0
5	36.16232919354489	37.0	37.0	37.0	35.0	37.0
6	36.30815685492415	37.0	37.0	37.0	35.0	37.0
7	36.24815211836414	37.0	37.0	37.0	35.0	37.0
8	36.22766584028346	37.0	37.0	37.0	35.0	37.0
9	37.79700133765178	39.0	38.0	39.0	35.0	39.0
10	37.60395076339308	39.0	37.0	39.0	35.0	39.0
11	37.2638562421235	39.0	37.0	39.0	35.0	39.0
12	37.27286161044353	39.0	37.0	39.0	35.0	39.0
13	37.25552691605989	39.0	37.0	39.0	35.0	39.0
14	38.592644358474935	40.0	38.0	41.0	35.0	41.0
15	38.62831715008663	40.0	38.0	41.0	35.0	41.0
16	38.46568355711721	40.0	37.0	41.0	34.0	41.0
17	38.4629029602188	40.0	37.0	41.0	34.0	41.0
18	38.29249340522583	40.0	37.0	41.0	34.0	41.0
19	38.073317291133776	40.0	37.0	41.0	34.0	41.0
20	37.84240113415027	40.0	35.0	41.0	34.0	41.0
21	37.75647619616181	40.0	35.0	41.0	34.0	41.0
22	37.697502415842415	40.0	35.0	41.0	34.0	41.0
23	37.713555549286795	40.0	35.0	41.0	34.0	41.0
24	37.62314104780317	39.0	35.0	41.0	34.0	41.0
25	37.50785501402927	39.0	35.0	41.0	34.0	41.0
26	37.41011786949268	39.0	35.0	41.0	33.0	41.0
27	37.43807603818901	39.0	35.0	41.0	33.0	41.0
28	37.406200366985	39.0	35.0	41.0	33.0	41.0
29	37.33868503384804	39.0	35.0	41.0	33.0	41.0
30	37.2861079056598	39.0	35.0	41.0	33.0	41.0
31	37.188912972598146	39.0	35.0	41.0	33.0	41.0
32	36.97637525888712	39.0	35.0	41.0	33.0	41.0
33	36.617704828339065	39.0	35.0	41.0	31.0	41.0
34	36.41256520150389	39.0	35.0	41.0	31.0	41.0
35	36.274181549327466	39.0	35.0	41.0	31.0	41.0
36	36.15159976986354	39.0	35.0	41.0	30.0	41.0
37	36.123170569299084	38.0	35.0	41.0	31.0	41.0
38	35.94846891681695	38.0	35.0	41.0	30.0	41.0
39	35.870375047644416	38.0	35.0	41.0	30.0	41.0
40	35.79068144141	38.0	35.0	41.0	30.0	41.0
41	35.771689279039144	38.0	35.0	41.0	30.0	41.0
42	35.70253943907846	38.0	35.0	40.0	30.0	41.0
43	35.61516532365728	37.0	35.0	40.0	30.0	41.0
44	35.463612788187206	37.0	35.0	40.0	30.0	41.0
45	35.373109722215844	37.0	35.0	40.0	29.0	41.0
46	35.32108070980173	37.0	35.0	40.0	29.0	41.0
47	35.2245326255172	36.0	35.0	40.0	29.0	41.0
48	35.10898024224684	36.0	35.0	40.0	29.0	41.0
49	35.08233610813527	36.0	35.0	40.0	29.0	41.0
50	34.984795445554816	36.0	35.0	40.0	28.0	41.0
51	34.868659520715234	35.0	35.0	40.0	28.0	41.0
52	34.51377243387677	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	0.0
12	4.0
13	14.0
14	32.0
15	73.0
16	132.0
17	272.0
18	622.0
19	1189.0
20	2228.0
21	3542.0
22	5730.0
23	8892.0
24	14420.0
25	24555.0
26	36918.0
27	45696.0
28	48914.0
29	49845.0
30	53226.0
31	60808.0
32	72692.0
33	95386.0
34	222242.0
35	300016.0
36	275818.0
37	302585.0
38	448205.0
39	972644.0
40	1925.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.565900835327124	17.01058116016855	23.852548656345515	28.57096934815881
2	33.88562585243319	20.377966992343435	24.321579623082663	21.41482753214071
3	27.021123614375785	21.03534510300706	32.228223468539596	19.715307814077555
4	23.951445667654873	23.63622169462571	29.862108372755465	22.550224264963955
5	22.2671459208181	32.20877208289898	26.953355380423837	18.57072661585908
6	79.73542835362554	2.1674026266258966	15.33513786210575	2.76203115764282
7	79.54783564792795	2.049316642972933	14.0562010558199	4.346646653279215
8	70.78319872624586	3.4190812516851854	19.296266580420163	6.5014534416487955
9	42.96220658093187	15.60129054859468	23.706384449912846	17.730118420560608
10	29.163334564489052	15.918417018027137	32.97383805032169	21.944410367162128
11	25.49479667233698	12.724027151903842	38.8474348772201	22.93374129853908
12	20.86106987213256	14.436437923182444	41.04809838924158	23.654393815443417
13	17.030393364092543	18.37178453506596	42.69090403348918	21.90691806735231
14	17.90646015614903	17.755703717018747	41.87102648865423	22.46680963817799
15	21.813826950239225	13.59796183592215	41.8560033274006	22.732207886438022
16	20.589373704744368	17.74658485494777	37.39583012150391	24.26821131880395
17	21.49961982873596	19.552972388216855	36.54711991566037	22.40028786738682
18	22.182386425884975	24.7963180790297	32.95717480596177	20.06412068912356
19	23.347829481215474	20.66717924730682	31.9414057349114	24.043585536566308
20	23.071869097750923	24.54158037096056	32.86431330048356	19.522237230804958
21	20.197754660624163	19.15971326099036	34.95387758288488	25.688654495500597
22	21.91649615269305	21.02858796060914	32.062870289763325	24.99204559693449
23	23.01902562006622	19.44190596025882	34.81076393102992	22.728304488645048
24	25.204108342577936	17.56391239856906	31.914377165319717	25.31760209353328
25	19.359245771701744	19.174572413933358	34.42071936669175	27.045462447673145
26	18.653452407740406	18.02142998190004	35.15373811021752	28.17137950014203
27	22.08312859629223	20.251221369889254	34.30755363235766	23.358096401460855
28	22.402255967114364	21.363361724265292	30.959094359229372	25.27528794939097
29	21.93935891119475	18.509912334277804	33.48111575509754	26.069612999429907
30	26.249366107879418	19.074461741125344	30.957159061163946	23.719013089831286
31	26.650464832353986	18.401043617682195	28.308851266111358	26.63964028385246
32	25.68829367721721	17.37786137099139	28.77384762840703	28.159997323384374
33	20.823971192268253	20.53856393011147	32.867724673344654	25.76974020427563
34	22.604740627417073	19.29242878595144	31.144915775172162	26.957914811459325
35	24.105285463024984	23.454762899745656	27.833292768611173	24.60665886861819
36	24.36336894063096	22.58230429052301	28.395808472406912	24.658518296439116
37	25.95155981743907	24.903317100884138	27.708220030925407	21.43690305075139
38	25.03800072557277	20.659339650058747	27.670432516156456	26.632227108212025
39	25.38979855187222	18.235624835581667	32.93949471007595	23.435081902470163
40	20.898660576928755	18.77255524291927	35.20215336351524	25.12663081663674
41	21.766526953453784	22.156079492860062	29.435129136863626	26.64226441682253
42	20.701292975917678	17.03898739956951	32.62889577140653	29.630823853106286
43	22.34127767722246	18.73030670210121	32.46196811284822	26.466447507828118
44	22.841732636276145	19.548117742222235	32.14395599853836	25.466193622963264
45	23.907819457027525	20.4622672640068	30.799547074649368	24.830366204316306
46	24.179482822753595	18.953226797908304	30.832119125140313	26.03517125419779
47	20.861430690415943	20.530265109593635	33.42600896272616	25.182295237264263
48	22.474977252047314	19.31411068461661	30.703766221241963	27.50714584209411
49	22.019722983403014	17.191023103522703	33.98629415349735	26.80295975957694
50	20.845620289271295	17.55111975033999	35.88557599390676	25.717683966481953
51	20.17216936416602	16.232558536206145	34.15666598657887	29.43860611304896
52	18.614648041445555	17.351226421345224	37.569416517473776	26.464709019735448
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	735.0
1	5432.0
2	10129.0
3	82914.5
4	155700.0
5	88758.5
6	21817.0
7	19983.0
8	18149.0
9	17576.0
10	17003.0
11	16522.5
12	16042.0
13	14986.0
14	13276.0
15	12622.0
16	11924.5
17	11227.0
18	10567.5
19	9908.0
20	9470.0
21	9032.0
22	8666.0
23	8300.0
24	8596.5
25	8893.0
26	9589.0
27	10285.0
28	10933.0
29	11581.0
30	13660.5
31	15740.0
32	18523.0
33	21306.0
34	24096.0
35	26886.0
36	29278.5
37	31671.0
38	34764.5
39	40840.5
40	43823.0
41	51922.5
42	60022.0
43	72651.0
44	85280.0
45	99368.5
46	113457.0
47	135945.5
48	158434.0
49	207329.0
50	256224.0
51	284311.5
52	312399.0
53	291969.5
54	271540.0
55	250641.0
56	229742.0
57	212979.0
58	196216.0
59	184689.0
60	173162.0
61	167711.0
62	162260.0
63	158344.5
64	132787.0
65	111145.0
66	93763.0
67	76381.0
68	64177.0
69	51973.0
70	44868.5
71	37764.0
72	37672.0
73	37580.0
74	30191.0
75	22802.0
76	17135.0
77	11468.0
78	9237.0
79	7006.0
80	5367.0
81	3728.0
82	2713.0
83	1698.0
84	1212.5
85	727.0
86	540.0
87	353.0
88	229.5
89	83.0
90	60.0
91	40.5
92	21.0
93	12.5
94	4.0
95	5.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3048626.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.448623208291718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.20885233237242	19.362733508503865
2	10.898206402634269	5.764851095789322
3	4.202049753294291	3.334152918821276
4	2.048611943532208	2.1673186157795827
5	1.2416236946954282	1.6419618633743207
6	0.8228655975920272	1.3058197285066393
7	0.5934472592162218	1.0987104067102291
8	0.4739393087522797	1.0028033760629822
9	0.39592899453932995	0.9424599114427673
>10	5.199309584876002	32.58858272057746
>50	0.7309632020323895	12.66121956937663
>100	0.1696271344841976	7.734189826750465
>500	0.007972535783465533	1.3858944053187499
>1k	0.005481118351132521	2.613872495387088
>5k	6.22854358083241E-4	1.0693039873673402
>10k+	4.982834864665928E-4	5.326125570231236
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	114861	3.767631713434183	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	23808	0.7809419718915996	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	12216	0.40070510452905667	No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	10771	0.35330670275724213	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT	8992	0.2949525458354026	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	6369	0.2089137860793682	Illumina Single End Adapter 2 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	6157	0.2019598337086937	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5931	0.19454665806825763	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	5006	0.16420512060187115	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4193	0.13753736929357685	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3917	0.12848411054684963	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	3864	0.126745622454181	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCG	3645	0.1195620584486257	No Hit
GGGCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	3177	0.10421088057374042	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	3069	0.10066830106415152	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.30807321068573185	0.0
2	0.0	0.0	0.0	1.0310874472631277	0.0
3	0.0	0.0	0.0	1.656418334029822	0.0
4	0.0	0.0	0.0	2.07172017820487	0.0
5	0.0	0.0	0.0	4.2647081012889085	0.0
6	0.0	0.0	0.0	4.962924281299182	0.0
7	0.0	0.0	0.0	7.062230657351869	0.0
8	3.280166212582324E-5	0.0	0.0	9.45091329667857	0.0
9	3.280166212582324E-5	0.0	0.0	11.66925690458587	0.0
10	3.280166212582324E-5	0.0	0.0	12.983291489346348	0.0
11	3.280166212582324E-5	0.0	0.0	14.110323798327508	0.0
12	3.280166212582324E-5	0.0	0.0	14.944273256214439	0.0
13	3.280166212582324E-5	0.0	0.0	15.363183283223327	0.0
14	6.560332425164648E-5	0.0	0.0	15.62546537358141	0.0
15	6.560332425164648E-5	0.0	0.0	16.328470596262054	0.0
16	6.560332425164648E-5	0.0	0.0	17.114431222458904	0.0
17	9.840498637746972E-5	0.0	0.0	18.094446481792126	0.0
18	9.840498637746972E-5	0.0	0.0	18.676905596160367	0.0
19	9.840498637746972E-5	0.0	0.0	19.3077471621642	0.0
20	9.840498637746972E-5	0.0	0.0	19.998484563209786	0.0
21	1.3120664850329295E-4	0.0	0.0	20.67911905232062	0.0
22	1.9680997275493944E-4	0.0	0.0	21.359195913175313	0.0
23	1.9680997275493944E-4	0.0	0.0	21.96376334781636	0.0
24	2.296116348807627E-4	0.0	0.0	22.461790983872735	0.0
25	2.296116348807627E-4	0.0	0.0	22.9415152924629	0.0
26	2.296116348807627E-4	0.0	0.0	23.416483360044822	0.0
27	2.296116348807627E-4	0.0	0.0	23.938587416101548	0.0
28	2.296116348807627E-4	0.0	0.0	24.438484746899096	0.0
29	2.296116348807627E-4	0.0	0.0	24.94914102287391	0.0
30	2.624132970065859E-4	0.0	0.0	25.42906214143683	0.0
31	2.624132970065859E-4	0.0	0.0	25.920431040081663	0.0
32	2.624132970065859E-4	0.0	0.0	26.467726772651023	0.0
33	2.9521495913240915E-4	0.0	0.0	26.976054130614905	0.0
34	3.280166212582324E-4	0.0	0.0	27.542604438852127	0.0
35	3.280166212582324E-4	0.0	0.0	28.043321811202816	0.0
36	3.280166212582324E-4	0.0	0.0	28.55148516085607	0.0
37	3.6081828338405564E-4	0.0	0.0	29.1672051606199	0.0
38	3.6081828338405564E-4	0.0	0.0	29.788009418013228	0.0
39	3.6081828338405564E-4	0.0	0.0	30.5587500729837	0.0
40	3.6081828338405564E-4	0.0	0.0	31.252341218634232	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	20	6.3131034E-4	46.0	1
GTAACGA	45	3.110472E-10	46.0	8
CGTTTTT	28340	0.0	45.326397	1
ACTTACT	6335	0.0	42.369377	37
CACTTAC	6415	0.0	42.342945	36
TCACGAC	610	0.0	42.229507	24
CGCACTT	6475	0.0	41.9861	34
TATACGG	170	0.0	41.941177	1
CCGCACT	6470	0.0	41.911903	33
GCACTTA	6550	0.0	41.50534	35
ACCCGCA	6630	0.0	41.420815	31
CCCGCAC	6665	0.0	41.375847	32
CTTACTG	6550	0.0	40.838165	38
ATCAACG	7605	0.0	40.798157	14
TCAACGC	7645	0.0	40.61478	15
TTATGAC	7400	0.0	40.591892	26
AACGCAA	7555	0.0	40.550632	17
GGACTTA	8255	0.0	40.511208	7
CTTAATC	8200	0.0	40.47439	10
CTTATGA	7385	0.0	40.45633	25
>>END_MODULE