FastQCFastQC Report
Thu 26 May 2016
SRR1043337_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043337_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2992071
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1101453.6812294895408564No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC228490.7636516646830908No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG105890.35390202973124635No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC101890.34053336301177345No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT86100.28776055113665416Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC65150.21774215919341486No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC59040.19732152077942003No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC58250.19468120910232412No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG50560.16897994733413746No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC41740.13950203721769971No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC38680.12927500717730295No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA36780.12292489048555333No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCC34280.11456947378588275No Hit
GGGCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC32030.10704959875617924No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA253.8923114E-545.025
CACGTAA253.8923114E-545.029
CGTTTTT269850.044.3162841
TATACGG2000.041.6251
CACGACG4850.040.82474525
CACTTAC56250.040.7636
AATACGG1550.040.645161
ACTTACT56400.040.49202337
ACCCGCA57400.040.2177731
CGCACTT57600.039.96093834
GCACTTA57700.039.93067635
CCGCACT57650.039.9262833
GCGATCG850.039.7058838
CCCGCAC58650.039.62915832
TAGTAGG6150.039.5121961
ATAGCGG4850.039.432991
CTTATGA64100.039.38377825
ACGATAC403.4607E-739.37500444
CTTACTG57950.039.37014838
GTTTTTT314600.039.3642732
Produced by FastQC (version 0.11.5)