##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043337_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2992071
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3317468068104	33.0	31.0	34.0	31.0	34.0
2	32.61751943720587	34.0	31.0	34.0	31.0	34.0
3	32.73090879193709	34.0	31.0	34.0	31.0	34.0
4	36.2046579108584	37.0	37.0	37.0	35.0	37.0
5	36.18900721272991	37.0	37.0	37.0	35.0	37.0
6	36.33768216061718	37.0	37.0	37.0	35.0	37.0
7	36.28883806567424	37.0	37.0	37.0	35.0	37.0
8	36.19414345448353	37.0	37.0	37.0	35.0	37.0
9	37.77523360909551	39.0	38.0	39.0	35.0	39.0
10	37.56681342120558	39.0	37.0	39.0	35.0	39.0
11	37.2718642037572	39.0	37.0	39.0	35.0	39.0
12	37.28420682530595	39.0	37.0	39.0	35.0	39.0
13	37.28226068164826	39.0	37.0	39.0	35.0	39.0
14	38.618213939441944	40.0	38.0	41.0	35.0	41.0
15	38.66643505451575	40.0	38.0	41.0	35.0	41.0
16	38.54819153689869	40.0	38.0	41.0	34.0	41.0
17	38.51930218233458	40.0	37.0	41.0	34.0	41.0
18	38.36516479722573	40.0	37.0	41.0	34.0	41.0
19	38.16600207682237	40.0	37.0	41.0	34.0	41.0
20	37.95446231055346	40.0	35.0	41.0	34.0	41.0
21	37.87337900738318	40.0	35.0	41.0	34.0	41.0
22	37.82699942614998	40.0	35.0	41.0	34.0	41.0
23	37.8105051651515	40.0	35.0	41.0	34.0	41.0
24	37.72191101080155	40.0	35.0	41.0	34.0	41.0
25	37.67555749846845	40.0	35.0	41.0	34.0	41.0
26	37.64603580596851	39.0	35.0	41.0	34.0	41.0
27	37.61897561922829	39.0	35.0	41.0	34.0	41.0
28	37.53329349470651	39.0	35.0	41.0	34.0	41.0
29	37.495295064856414	39.0	35.0	41.0	34.0	41.0
30	37.39730474310269	39.0	35.0	41.0	33.0	41.0
31	37.28543473734413	39.0	35.0	41.0	33.0	41.0
32	37.08670984077584	39.0	35.0	41.0	33.0	41.0
33	36.715903800411155	39.0	35.0	41.0	32.0	41.0
34	36.473132154952204	39.0	35.0	41.0	31.0	41.0
35	36.32143889633635	39.0	35.0	41.0	31.0	41.0
36	36.18980732743307	39.0	35.0	41.0	31.0	41.0
37	36.10547376716662	38.0	35.0	41.0	31.0	41.0
38	35.94356450766041	38.0	35.0	41.0	30.0	41.0
39	35.826882450316184	38.0	35.0	41.0	30.0	41.0
40	35.72478026089622	38.0	35.0	40.0	30.0	41.0
41	35.66062068714278	38.0	35.0	40.0	30.0	41.0
42	35.570910917555096	37.0	35.0	40.0	30.0	41.0
43	35.47341690755333	37.0	35.0	40.0	30.0	41.0
44	35.375368097882706	37.0	35.0	40.0	29.0	41.0
45	35.31159487859747	37.0	35.0	40.0	29.0	41.0
46	35.20498945379304	37.0	35.0	40.0	29.0	41.0
47	35.05645153473965	36.0	35.0	40.0	28.0	41.0
48	34.978181667480484	36.0	35.0	40.0	28.0	41.0
49	34.93164366754666	36.0	35.0	40.0	28.0	41.0
50	34.86532405146803	36.0	35.0	40.0	28.0	41.0
51	34.495329489173216	35.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	5.0
13	5.0
14	14.0
15	55.0
16	117.0
17	271.0
18	538.0
19	1011.0
20	1893.0
21	3152.0
22	4963.0
23	7687.0
24	12379.0
25	21257.0
26	33997.0
27	43895.0
28	48011.0
29	48716.0
30	51877.0
31	59234.0
32	69412.0
33	91432.0
34	214494.0
35	298679.0
36	265508.0
37	299584.0
38	464154.0
39	949574.0
40	155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.838238798477708	17.13371774934485	23.805718514032588	28.22232493814485
2	33.63299199785032	20.43928770406852	24.43267556150907	21.495044736572094
3	27.053101346859748	21.09331630165193	32.00719501642842	19.846387335059898
4	24.13057711531578	23.74402211712222	29.550969880059665	22.574430887502338
5	22.400070051813607	32.2966934942386	26.68345771206632	18.61977874188146
6	80.03917687782142	2.1277904167381054	15.067022139514737	2.7660105659257415
7	79.8288877503241	2.033942376367406	13.77600999441524	4.361159878893249
8	71.06047951402222	3.369706133310339	19.06191397196123	6.507900380706206
9	43.15338773712255	15.589269104910947	23.415453710824373	17.84188944714213
10	29.42847947124249	15.884582952744102	32.65600983399124	22.030927742022165
11	25.705740271537675	12.701302876836811	38.65793960103219	22.93501725059332
12	21.058758298182095	14.397786683537925	40.88161677981572	23.661838238464263
13	17.074227182443195	18.34913008414573	42.54126990970468	22.03537282370639
14	18.04459185627614	17.67224106647202	41.73911648486951	22.544050592382334
15	21.955662148391532	13.583835410322816	41.67387739127848	22.78662505000717
16	20.691721553398967	17.695736498231494	37.306434239027084	24.30610770934246
17	21.68935830733963	19.43466582176693	36.41882161218768	22.457154258705756
18	22.30826073311763	24.908399566721513	32.79768427955085	19.985655420610005
19	23.469496546037842	20.71795756183593	31.722342150303252	24.09020374182297
20	23.10690488293894	24.663051110752384	32.63391811223731	19.596125894071363
21	20.295607958500987	19.212010677554108	34.695667315381215	25.796714048563686
22	21.992091765202098	21.107386823374178	31.807767930640686	25.092753480783042
23	23.161047983152805	19.427480163405214	34.56569045320114	22.845781400240835
24	25.256619913096984	17.567698092725742	31.708839796916582	25.466842197260693
25	19.32277008132494	19.237444565987907	34.312554748867925	27.12723060381923
26	18.69862045385955	17.92474175913606	35.04515768509504	28.331480101909346
27	22.063580710484477	20.249619744985996	34.184683451696166	23.502116092833358
28	22.474232730439887	21.380575527786608	30.814542836717447	25.330648905056062
29	22.0173251236351	18.46456852126838	33.355892958422444	26.162213396674076
30	26.3645147458065	19.037349046864197	30.747699503120078	23.850436704209223
31	26.77834182410778	18.39866099434138	28.057288747492958	26.765708434057885
32	25.81349172529663	17.266034128200836	28.572684271195435	28.347789875307107
33	20.87477202245535	20.475182574210304	32.65039499396906	25.999650409365287
34	22.711493143043732	19.209370365877014	31.01440440417356	27.06473208690569
35	24.163330348778487	23.393729627405232	27.68320003101531	24.759739992800974
36	24.33909489447276	22.57362876749917	28.20812072975541	24.879155608272665
37	26.06595899629387	24.784472026232	27.616858022419922	21.532710955054206
38	25.226707521312164	20.598475103030644	27.505998353648693	26.6688190220085
39	25.56513531931562	18.17590558512816	32.826560599664916	23.432398495891306
40	21.04365170478909	18.67178285542021	35.06935497185729	25.215210467933414
41	21.91622458156909	22.073841162191673	29.305554580756944	26.704379675482297
42	20.772735673718973	16.969985003698106	32.560490710280604	29.696788612302317
43	22.49819606553454	18.60884985683829	32.33683291606383	26.556121161563347
44	22.962456439035037	19.362642129815768	32.05244795327384	25.622453477875357
45	24.056782075024287	20.309143733554453	30.779951411580807	24.854122779840452
46	24.318807942725957	18.829165484375203	30.7472984431185	26.10472812978034
47	21.069185858223285	20.31469173024303	33.36351309845254	25.25260931308114
48	22.64080631776452	19.090556340407698	30.649740597733143	27.61889674409464
49	22.13376621076171	17.013299483869197	33.93488991404281	26.918044391326273
50	21.0130374580015	17.392501715367047	35.80048735474526	25.793973471886193
51	20.328862516965675	16.176755163898182	33.96750946083833	29.526872858297814
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	657.0
1	5202.5
2	9748.0
3	79125.0
4	148502.0
5	85366.5
6	22231.0
7	20022.5
8	17814.0
9	17192.0
10	16570.0
11	16042.5
12	15515.0
13	14598.0
14	13681.0
15	12765.5
16	11850.0
17	11150.5
18	10451.0
19	9724.0
20	8997.0
21	8714.5
22	8432.0
23	8226.5
24	8021.0
25	8095.5
26	9190.0
27	10210.0
28	11478.5
29	12747.0
30	14583.0
31	16419.0
32	19590.5
33	22762.0
34	25027.5
35	27293.0
36	30800.0
37	34307.0
38	36162.5
39	38018.0
40	44166.5
41	50315.0
42	59631.0
43	68947.0
44	80663.0
45	92379.0
46	105927.5
47	119476.0
48	160267.5
49	201059.0
50	245667.5
51	290276.0
52	300377.0
53	310478.0
54	283297.0
55	256116.0
56	233876.0
57	211636.0
58	195832.0
59	180028.0
60	174203.5
61	168379.0
62	160094.5
63	151810.0
64	144822.0
65	137834.0
66	114966.5
67	92099.0
68	75966.5
69	59834.0
70	51633.0
71	43432.0
72	40589.5
73	37747.0
74	32542.5
75	20971.0
76	14604.0
77	11155.5
78	7707.0
79	6031.0
80	4355.0
81	3237.5
82	2120.0
83	1555.0
84	990.0
85	721.0
86	452.0
87	303.0
88	154.0
89	116.0
90	78.0
91	48.0
92	18.0
93	14.0
94	10.0
95	6.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2992071.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.14964756522194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.69390456081055	19.009199844050755
2	11.035558136808982	5.771519119261442
3	4.218736034310928	3.3095538140379848
4	2.107220179381619	2.204122601326124
5	1.277432215987131	1.6702201118261972
6	0.8367022291300862	1.3127681045272395
7	0.6341637474932844	1.1608210946912851
8	0.48641418242031453	1.0175647552813438
9	0.3979096251327346	0.9364676814027528
>10	5.418629289226321	33.47840896124199
>50	0.7068361369018059	12.097476916689459
>100	0.17232167876175752	7.8789260846563955
>500	0.007730172891577046	1.3704169212011852
>1k	0.005282284809244307	2.5268672798105163
>5k	6.441810742980862E-4	1.0750547964581894
>10k+	5.153448594384689E-4	5.180611913537096
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	110145	3.6812294895408564	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	22849	0.7636516646830908	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	10589	0.35390202973124635	No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	10189	0.34053336301177345	No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	8610	0.28776055113665416	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	6515	0.21774215919341486	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5904	0.19732152077942003	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5825	0.19468120910232412	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	5056	0.16897994733413746	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4174	0.13950203721769971	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3868	0.12927500717730295	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	3678	0.12292489048555333	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCC	3428	0.11456947378588275	No Hit
GGGCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	3203	0.10704959875617924	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2990904961814075	0.0
2	0.0	0.0	0.0	1.020463752364165	0.0
3	0.0	0.0	0.0	1.657046239878666	0.0
4	0.0	0.0	0.0	2.0819358898903135	0.0
5	0.0	3.342166679868225E-5	0.0	4.304744105337073	0.0
6	0.0	3.342166679868225E-5	0.0	5.005730144772634	0.0
7	0.0	3.342166679868225E-5	0.0	7.142544411546384	0.0
8	0.0	3.342166679868225E-5	0.0	9.564445496112892	0.0
9	3.342166679868225E-5	3.342166679868225E-5	0.0	11.85830817517365	0.0
10	3.342166679868225E-5	3.342166679868225E-5	0.0	13.232206053933881	0.0
11	3.342166679868225E-5	3.342166679868225E-5	0.0	14.38522013682162	0.0
12	3.342166679868225E-5	3.342166679868225E-5	0.0	15.225775056808478	0.0
13	3.342166679868225E-5	3.342166679868225E-5	0.0	15.660089616857354	0.0
14	3.342166679868225E-5	3.342166679868225E-5	0.0	15.929434829587935	0.0
15	3.342166679868225E-5	3.342166679868225E-5	0.0	16.65902981580317	0.0
16	3.342166679868225E-5	3.342166679868225E-5	0.0	17.472011860681114	0.0
17	3.342166679868225E-5	3.342166679868225E-5	0.0	18.46877965128501	0.0
18	3.342166679868225E-5	3.342166679868225E-5	0.0	19.069868328659314	0.0
19	3.342166679868225E-5	3.342166679868225E-5	0.0	19.714371751205103	0.0
20	3.342166679868225E-5	3.342166679868225E-5	0.0	20.41976945065809	0.0
21	3.342166679868225E-5	3.342166679868225E-5	0.0	21.12433160844111	0.0
22	6.68433335973645E-5	3.342166679868225E-5	0.0	21.811581342822414	0.0
23	6.68433335973645E-5	3.342166679868225E-5	0.0	22.440309738639222	0.0
24	1.33686667194729E-4	3.342166679868225E-5	0.0	22.948486182313186	0.0
25	1.33686667194729E-4	3.342166679868225E-5	0.0	23.43356825422926	0.0
26	1.33686667194729E-4	3.342166679868225E-5	0.0	23.913570232791937	0.0
27	1.33686667194729E-4	3.342166679868225E-5	0.0	24.44276890488227	0.0
28	1.33686667194729E-4	3.342166679868225E-5	0.0	24.949675325217886	0.0
29	1.6710833399341126E-4	3.342166679868225E-5	0.0	25.47322573561924	0.0
30	1.6710833399341126E-4	3.342166679868225E-5	0.0	25.967465344238153	0.0
31	1.6710833399341126E-4	3.342166679868225E-5	0.0	26.459298592847563	0.0
32	1.6710833399341126E-4	3.342166679868225E-5	0.0	27.022052618403773	0.0
33	1.6710833399341126E-4	3.342166679868225E-5	0.0	27.528290605403413	0.0
34	1.6710833399341126E-4	3.342166679868225E-5	0.0	28.090610149291244	0.0
35	1.6710833399341126E-4	3.342166679868225E-5	0.0	28.576728292878077	0.0
36	1.6710833399341126E-4	3.342166679868225E-5	0.0	29.08189678654016	0.0
37	1.6710833399341126E-4	3.342166679868225E-5	0.0	29.700866055651755	0.0
38	1.6710833399341126E-4	3.342166679868225E-5	0.0	30.32712124812546	0.0
39	1.6710833399341126E-4	3.342166679868225E-5	0.0	31.096822234499115	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATA	25	3.8923114E-5	45.0	25
CACGTAA	25	3.8923114E-5	45.0	29
CGTTTTT	26985	0.0	44.316284	1
TATACGG	200	0.0	41.625	1
CACGACG	485	0.0	40.824745	25
CACTTAC	5625	0.0	40.76	36
AATACGG	155	0.0	40.64516	1
ACTTACT	5640	0.0	40.492023	37
ACCCGCA	5740	0.0	40.21777	31
CGCACTT	5760	0.0	39.960938	34
GCACTTA	5770	0.0	39.930676	35
CCGCACT	5765	0.0	39.92628	33
GCGATCG	85	0.0	39.705883	8
CCCGCAC	5865	0.0	39.629158	32
TAGTAGG	615	0.0	39.512196	1
ATAGCGG	485	0.0	39.43299	1
CTTATGA	6410	0.0	39.383778	25
ACGATAC	40	3.4607E-7	39.375004	44
CTTACTG	5795	0.0	39.370148	38
GTTTTTT	31460	0.0	39.364273	2
>>END_MODULE