FastQCFastQC Report
Thu 26 May 2016
SRR1043336_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043336_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1100797
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT668426.072145908827877No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT94470.8581963795322843TruSeq Adapter, Index 14 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCTT85060.7727128616811274Illumina PCR Primer Index 3 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT53210.48337704408714777TruSeq Adapter, Index 14 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC39970.36310055350804915Illumina PCR Primer Index 3 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCG34920.31722470173883105No Hit
TCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT32960.2994194206561246TruSeq Adapter, Index 14 (96% over 26bp)
ACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC24520.22274770007549077Illumina PCR Primer Index 3 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC22130.20103615834708854TruSeq Adapter, Index 14 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT20480.18604701866011625Illumina PCR Primer Index 3 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC19420.17641763195212198Illumina PCR Primer Index 3 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC18660.1695135433690317Illumina PCR Primer Index 3 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT18070.16415379039005376Illumina PCR Primer Index 3 (95% over 24bp)
TGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCTTG15210.13817261493263516TruSeq Adapter, Index 14 (96% over 28bp)
TGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC14310.12999672055792302Illumina PCR Primer Index 3 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG13490.12254757234985197Illumina PCR Primer Index 3 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC13190.11982227422494791No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT12960.11773287899585483No Hit
GGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC12640.11482589432929051TruSeq Adapter, Index 14 (96% over 25bp)
CGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG12560.11409914816264942Illumina PCR Primer Index 3 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC11780.1070133730378989Illumina PCR Primer Index 3 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT11280.10247120949639216TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCC405.6115823E-946.0000045
GAGTGCG206.311336E-446.00000435
ACCGGGT351.0195254E-746.0000043
ACACGGC206.311336E-446.0000042
TCCGCGG351.0195254E-746.0000041
CTCGTGA700.046.00000416
ACGCAGA206.311336E-446.00000410
GGCCGAG206.311336E-446.0000048
CCGTGCA206.311336E-446.00000414
ACGATGG551.8189894E-1246.0000041
CCCGTGC206.311336E-446.00000413
GTCACGG700.046.0000041
ACGTGTA206.311336E-446.00000420
CGCAGAT206.311336E-446.00000411
CGGTCAC206.311336E-446.00000417
GTAAGCG206.311336E-446.00000416
TAAGCGT206.311336E-446.00000417
AATACGG405.6115823E-946.0000041
GCCCGTT206.311336E-446.00000435
GCTCCGT206.311336E-446.00000420
Produced by FastQC (version 0.11.5)