##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043336_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1100797
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.254747242225406	33.0	31.0	34.0	31.0	34.0
2	32.59427487538574	34.0	31.0	34.0	31.0	34.0
3	32.6698437586585	34.0	31.0	34.0	31.0	34.0
4	36.19031756082184	37.0	35.0	37.0	35.0	37.0
5	36.08620935558509	37.0	35.0	37.0	35.0	37.0
6	36.32467112464878	37.0	37.0	37.0	35.0	37.0
7	36.24545670091761	37.0	37.0	37.0	35.0	37.0
8	36.2256156221356	37.0	37.0	37.0	35.0	37.0
9	37.804856844631665	39.0	38.0	39.0	35.0	39.0
10	37.614602874099404	39.0	37.0	39.0	35.0	39.0
11	37.314890029678494	39.0	37.0	39.0	35.0	39.0
12	37.32851833716843	39.0	37.0	39.0	34.0	39.0
13	37.32858919491968	39.0	37.0	39.0	34.0	39.0
14	38.73154541663904	40.0	38.0	41.0	35.0	41.0
15	38.83839708865486	41.0	38.0	41.0	35.0	41.0
16	38.873785993239444	41.0	38.0	41.0	35.0	41.0
17	38.85999780159285	41.0	38.0	41.0	35.0	41.0
18	38.59099270801065	40.0	38.0	41.0	35.0	41.0
19	38.302969575680166	40.0	37.0	41.0	35.0	41.0
20	38.00849384582262	40.0	35.0	41.0	34.0	41.0
21	37.94177309712872	40.0	35.0	41.0	34.0	41.0
22	37.91045760480815	40.0	35.0	41.0	34.0	41.0
23	37.897622359072564	40.0	35.0	41.0	34.0	41.0
24	37.81462612997674	40.0	35.0	41.0	34.0	41.0
25	37.740338136822686	40.0	35.0	41.0	34.0	41.0
26	37.689330548684275	40.0	35.0	41.0	34.0	41.0
27	37.72794893154687	40.0	35.0	41.0	34.0	41.0
28	37.75658727267607	40.0	35.0	41.0	34.0	41.0
29	37.679189714361506	40.0	35.0	41.0	34.0	41.0
30	37.61565211387749	40.0	35.0	41.0	34.0	41.0
31	37.48029745720601	40.0	35.0	41.0	33.0	41.0
32	37.26086917024665	40.0	35.0	41.0	33.0	41.0
33	37.009111580064264	40.0	35.0	41.0	33.0	41.0
34	36.81752948091247	40.0	35.0	41.0	32.0	41.0
35	36.58609716414561	40.0	35.0	41.0	31.0	41.0
36	36.52378958154864	40.0	35.0	41.0	31.0	41.0
37	36.419434282615235	39.0	35.0	41.0	31.0	41.0
38	36.34213029286962	39.0	35.0	41.0	31.0	41.0
39	36.2937589764507	39.0	35.0	41.0	30.0	41.0
40	36.227392516513035	39.0	35.0	41.0	30.0	41.0
41	36.21158942111943	39.0	35.0	41.0	30.0	41.0
42	36.08458235260452	39.0	35.0	41.0	30.0	41.0
43	35.899792604812696	39.0	35.0	41.0	30.0	41.0
44	35.72389823010055	39.0	35.0	41.0	29.0	41.0
45	35.64410422630149	39.0	35.0	41.0	28.0	41.0
46	35.59254521950914	38.0	35.0	41.0	27.0	41.0
47	35.493091823469726	38.0	35.0	40.0	27.0	41.0
48	35.50656660583196	38.0	35.0	41.0	27.0	41.0
49	35.40430615272389	38.0	35.0	40.0	26.0	41.0
50	35.240948149386305	38.0	35.0	40.0	26.0	41.0
51	35.125400051053916	38.0	35.0	40.0	26.0	41.0
52	34.8254891683026	38.0	35.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	5.0
14	9.0
15	9.0
16	36.0
17	74.0
18	166.0
19	342.0
20	588.0
21	1129.0
22	1800.0
23	3264.0
24	6256.0
25	11210.0
26	15850.0
27	17186.0
28	15344.0
29	15152.0
30	16474.0
31	19425.0
32	24214.0
33	32119.0
34	57549.0
35	104583.0
36	108480.0
37	83522.0
38	138594.0
39	426701.0
40	715.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.26714734869372	20.36515361142881	26.198472561244262	28.16922647863321
2	33.84311548814177	19.761409233491733	26.549400116461076	19.846075161905418
3	20.449910383113327	21.774950331441673	38.685425196471286	19.089714088973718
4	22.18937733296875	23.30765799688771	36.742196790143865	17.760767879999673
5	19.905577504299156	29.222099987554472	31.822034398712933	19.05028810943344
6	71.51736423700282	1.4135212941168989	22.90967362738089	4.159440841499387
7	72.02881185177648	1.5278929720920387	18.614512939261278	7.828782236870195
8	58.62606820331088	3.402716395484363	27.948113957432657	10.023101443772104
9	33.51199176596593	17.777755571644906	30.085383590253244	18.624869072135915
10	25.85962716104786	18.482971883099246	43.81507217043651	11.842328785416385
11	22.692921583180187	15.855239431066764	40.92752796382984	20.524311021923207
12	17.781480145748944	19.681648841702877	44.71996199117548	17.816909021372695
13	13.358502975571335	23.190379334246007	43.148555092355814	20.30256259782685
14	11.46287644315891	23.08681800549965	44.59378068799243	20.85652486334901
15	12.06198781428365	18.355337087582907	48.898479919549196	20.684195178584243
16	12.341330872086315	25.323470176608403	39.61157234258451	22.723626608720775
17	14.091153954816374	22.440831506626562	41.51228609816342	21.955728440393642
18	16.123681296369814	28.860907142733854	39.72594402055965	15.289467540336682
19	16.131221287848714	23.144140109393465	36.73393005249832	23.990708550259495
20	13.457249610963693	30.111728138793982	35.621645044454155	20.80937720578817
21	13.943351953175744	24.657952374506834	37.20304470306514	24.195650969252277
22	12.10849956894868	26.71700595114267	32.302595301404345	28.8718991785043
23	11.52882865778159	23.57119432556593	43.81906927435304	21.08090774229944
24	16.66220020585085	21.74542626842188	39.05406718949997	22.538306336227297
25	12.212696800590845	26.54794662412779	38.753739336135546	22.48561723914582
26	14.127127890065108	23.431114001945865	37.71549159381794	24.726266514171098
27	19.54411212966605	23.466452034298786	39.771456499245545	17.217979336789618
28	14.21115791558298	22.806293985176197	36.91988622788761	26.06266187135321
29	17.424102718303196	26.348999861009798	34.308414721333726	21.918482699353287
30	20.579180357504608	21.561650331532515	35.13427089645048	22.724898414512396
31	18.907300801146807	23.183475245662915	35.55414849422737	22.355075458962915
32	20.24315109870394	24.254789938562695	34.89517140762556	20.6068875551078
33	13.281013665553232	20.39331502538615	40.39100760630706	25.934663702753554
34	21.523314471242198	17.857879336517087	39.65799325397871	20.960812938262006
35	20.320549565451216	18.456990707641825	39.805068509452695	21.417391217454263
36	21.358615621227166	19.141222223534403	32.88671753284211	26.613444622396322
37	20.649220519314643	18.285569455585364	40.89082728241447	20.174382742685527
38	18.44509023916308	18.521761959743714	35.874643553716076	27.15850424737713
39	22.623244794453473	18.01040518824088	35.82531565765532	23.541034359650325
40	15.545918093890155	16.974882744048177	41.18543200971659	26.293767152345072
41	18.553012044909277	21.358888151039658	34.53107157813838	25.55702822591268
42	19.359700289880877	16.447537556879244	40.431705391638964	23.761056761600912
43	22.665305228847828	19.64830936130822	35.7764419779487	21.909943431895254
44	17.25204556335092	22.38641638739931	35.83848793192569	24.523050117324086
45	19.612062896246993	19.398399523254515	36.60856633875274	24.38097124174575
46	24.16431004081588	16.95734999277796	35.787343170448324	23.09099679595784
47	16.44572069146264	15.577713238680701	41.18561369625826	26.790952373598405
48	18.323360256250698	14.105598034878367	34.58503248101149	32.986009227859455
49	20.743515834436323	14.356688835452857	40.43315888397225	24.46663644613857
50	18.577539728033415	13.923275590322284	40.549256584093165	26.94992809755114
51	16.328805401904255	13.63721013047819	38.69432783701264	31.339656630604917
52	14.59933121184015	13.795095735180965	48.173732304866384	23.431840748112503
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	530.0
1	1773.0
2	3016.0
3	38655.5
4	74295.0
5	41150.5
6	8006.0
7	7757.5
8	7509.0
9	7837.0
10	8165.0
11	8373.5
12	8582.0
13	8328.5
14	7571.5
15	7068.0
16	6464.0
17	5860.0
18	5353.5
19	4847.0
20	4558.0
21	4269.0
22	3959.5
23	3650.0
24	3673.0
25	3696.0
26	4231.5
27	4767.0
28	4616.5
29	4466.0
30	5056.5
31	5647.0
32	7392.5
33	9138.0
34	10227.5
35	11317.0
36	13637.5
37	15958.0
38	18192.0
39	22159.0
40	23892.0
41	33058.5
42	42225.0
43	54539.0
44	66853.0
45	90545.0
46	114237.0
47	142991.5
48	171746.0
49	175663.5
50	179581.0
51	151432.0
52	123283.0
53	95507.0
54	67731.0
55	51537.5
56	35344.0
57	27381.5
58	19419.0
59	15700.0
60	11981.0
61	10460.0
62	8939.0
63	7189.5
64	4405.0
65	3370.0
66	2773.5
67	2177.0
68	1751.5
69	1326.0
70	1312.5
71	1299.0
72	1175.0
73	1051.0
74	919.0
75	787.0
76	581.0
77	375.0
78	298.0
79	221.0
80	170.0
81	119.0
82	74.0
83	29.0
84	24.0
85	19.0
86	33.5
87	48.0
88	29.0
89	7.5
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1100797.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.517784573979363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.18137868859365	21.01116601808753
2	12.411021830341399	7.326917374619449
3	5.360871974544561	4.747231922198692
4	2.7713487611467094	3.272163028435726
5	1.6599543110946104	2.4499086878769516
6	1.0500090149640675	1.8596363942667369
7	0.7057806325713781	1.4583156468110174
8	0.518123820509989	1.2235093853168801
9	0.41485308260010656	1.102098952983709
>10	3.2670504510475125	20.828704310288686
>50	0.41422258280624696	8.225753320574707
>100	0.22624791809900674	12.571582763934252
>500	0.011729087159237218	2.3438868424669104
>1k	0.006173203768019589	3.368688179793654
>5k	9.259805652029382E-4	2.120485981947247
>10k+	3.0866018840097943E-4	6.089951190397777
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	66842	6.072145908827877	No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	9447	0.8581963795322843	TruSeq Adapter, Index 14 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCTT	8506	0.7727128616811274	Illumina PCR Primer Index 3 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	5321	0.48337704408714777	TruSeq Adapter, Index 14 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	3997	0.36310055350804915	Illumina PCR Primer Index 3 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCG	3492	0.31722470173883105	No Hit
TCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	3296	0.2994194206561246	TruSeq Adapter, Index 14 (96% over 26bp)
ACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	2452	0.22274770007549077	Illumina PCR Primer Index 3 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	2213	0.20103615834708854	TruSeq Adapter, Index 14 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT	2048	0.18604701866011625	Illumina PCR Primer Index 3 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1942	0.17641763195212198	Illumina PCR Primer Index 3 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1866	0.1695135433690317	Illumina PCR Primer Index 3 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	1807	0.16415379039005376	Illumina PCR Primer Index 3 (95% over 24bp)
TGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCTTG	1521	0.13817261493263516	TruSeq Adapter, Index 14 (96% over 28bp)
TGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1431	0.12999672055792302	Illumina PCR Primer Index 3 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1349	0.12254757234985197	Illumina PCR Primer Index 3 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC	1319	0.11982227422494791	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT	1296	0.11773287899585483	No Hit
GGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1264	0.11482589432929051	TruSeq Adapter, Index 14 (96% over 25bp)
CGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1256	0.11409914816264942	Illumina PCR Primer Index 3 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1178	0.1070133730378989	Illumina PCR Primer Index 3 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT	1128	0.10247120949639216	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7994207833051871	0.0
2	0.0	0.0	0.0	2.6552579630940127	0.0
3	0.0	0.0	0.0	4.477392289404858	0.0
4	0.0	0.0	0.0	5.654721079363407	0.0
5	0.0	0.0	0.0	11.380572439786809	0.0
6	0.0	0.0	0.0	12.7554853438009	0.0
7	0.0	0.0	0.0	17.445632573489934	0.0
8	0.0	0.0	0.0	23.157403226934665	0.0
9	0.0	0.0	0.0	27.297494451747234	0.0
10	0.0	0.0	0.0	29.95556855623698	0.0
11	0.0	0.0	0.0	32.37517907479762	0.0
12	0.0	0.0	0.0	34.08203329042503	0.0
13	0.0	0.0	0.0	34.79369947410831	0.0
14	0.0	0.0	0.0	35.286070002007634	0.0
15	0.0	0.0	0.0	36.85566003541071	0.0
16	0.0	0.0	0.0	38.733844659823745	0.0
17	0.0	0.0	0.0	40.82433000816681	0.0
18	0.0	0.0	0.0	41.937977665273436	0.0
19	9.084327083013489E-5	0.0	0.0	43.09959056937837	0.0
20	9.084327083013489E-5	0.0	0.0	44.39238115656202	0.0
21	2.725298124904047E-4	0.0	0.0	45.58987715264486	0.0
22	7.267461666410791E-4	0.0	0.0	46.75921173477035	0.0
23	0.0018168654166026978	0.0	0.0	47.645115311905826	0.0
24	0.0034520442915451257	0.0	0.0	48.37195232181774	0.0
25	0.0034520442915451257	0.0	0.0	49.05345853958541	0.0
26	0.0034520442915451257	0.0	0.0	49.74868209124843	0.0
27	0.0034520442915451257	0.0	0.0	50.41492663951664	0.0
28	0.0034520442915451257	0.0	0.0	51.099248998680046	0.0
29	0.0035428875623752607	0.0	0.0	51.90584640038082	0.0
30	0.0035428875623752607	0.0	0.0	52.61896607639737	0.0
31	0.0035428875623752607	0.0	0.0	53.218804193688754	0.0
32	0.0035428875623752607	0.0	0.0	53.8290892871256	0.0
33	0.0035428875623752607	0.0	0.0	54.398585751959715	0.0
34	0.0035428875623752607	0.0	0.0	55.071280172456866	0.0
35	0.0035428875623752607	0.0	0.0	55.621881236958316	0.0
36	0.0035428875623752607	0.0	0.0	56.13523656041941	0.0
37	0.0035428875623752607	0.0	0.0	56.68120461810852	0.0
38	0.0035428875623752607	0.0	0.0	57.178298996091016	0.0
39	0.0035428875623752607	0.0	0.0	57.712366585301375	0.0
40	0.0036337308332053957	0.0	0.0	58.25606356121973	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCC	40	5.6115823E-9	46.000004	5
GAGTGCG	20	6.311336E-4	46.000004	35
ACCGGGT	35	1.0195254E-7	46.000004	3
ACACGGC	20	6.311336E-4	46.000004	2
TCCGCGG	35	1.0195254E-7	46.000004	1
CTCGTGA	70	0.0	46.000004	16
ACGCAGA	20	6.311336E-4	46.000004	10
GGCCGAG	20	6.311336E-4	46.000004	8
CCGTGCA	20	6.311336E-4	46.000004	14
ACGATGG	55	1.8189894E-12	46.000004	1
CCCGTGC	20	6.311336E-4	46.000004	13
GTCACGG	70	0.0	46.000004	1
ACGTGTA	20	6.311336E-4	46.000004	20
CGCAGAT	20	6.311336E-4	46.000004	11
CGGTCAC	20	6.311336E-4	46.000004	17
GTAAGCG	20	6.311336E-4	46.000004	16
TAAGCGT	20	6.311336E-4	46.000004	17
AATACGG	40	5.6115823E-9	46.000004	1
GCCCGTT	20	6.311336E-4	46.000004	35
GCTCCGT	20	6.311336E-4	46.000004	20
>>END_MODULE