SRR1043335_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043335_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1082208
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61532	5.685783139655223	No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	9053	0.8365305006061681	TruSeq Adapter, Index 14 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	8543	0.7894046246192968	Illumina PCR Primer Index 3 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	5442	0.5028608178834384	TruSeq Adapter, Index 14 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	3902	0.3605591531387682	Illumina PCR Primer Index 3 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	3240	0.29938791803424114	TruSeq Adapter, Index 14 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC	3082	0.28478813684615156	No Hit
ACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	2516	0.23248765486856499	Illumina PCR Primer Index 3 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	2271	0.20984875365918565	TruSeq Adapter, Index 14 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	2045	0.1889655223395133	Illumina PCR Primer Index 3 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	1948	0.18000236553416718	No Hit
TGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCTT	1870	0.17279487861852805	TruSeq Adapter, Index 14 (96% over 27bp)
TCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1831	0.16919113516070847	Illumina PCR Primer Index 3 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1785	0.1649405659540495	Illumina PCR Primer Index 3 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1368	0.12640823205890178	Illumina PCR Primer Index 3 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT	1339	0.12372852538513852	Illumina PCR Primer Index 3 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT	1327	0.12261968124427099	Illumina PCR Primer Index 3 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGC	1323	0.12225006653064845	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTC	1307	0.12077160767615838	No Hit
GGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1260	0.11642863479109378	TruSeq Adapter, Index 14 (95% over 24bp)
ATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1133	0.10469336763357875	Illumina PCR Primer Index 3 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT	1107	0.10229087199503238	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGTCG	110	0.0	45.000004	5
TTGACGG	110	0.0	45.000004	1
TCATCGG	55	1.8189894E-12	45.000004	1
TATACGG	75	0.0	45.000004	1
TTACCGG	55	1.8189894E-12	45.000004	1
CGGGTAG	50	2.1827873E-11	45.0	5
GTCGCAA	35	1.2118471E-7	45.0	8
TCCGCGG	45	3.8562575E-10	45.0	1
CACGGTC	25	3.890654E-5	45.0	23
GGTCGAA	20	7.033105E-4	45.0	7
CCTACGG	35	1.2118471E-7	45.0	1
CGATTAC	25	3.890654E-5	45.0	9
TCGATGG	80	0.0	45.0	1
TGACGAT	20	7.033105E-4	45.0	14
AGCGGCA	20	7.033105E-4	45.0	3
TCAATCG	20	7.033105E-4	45.0	41
TACGAGG	195	0.0	45.0	1
CTTGCGG	180	0.0	45.0	1
GGTATCG	25	3.890654E-5	45.0	7
AGTTCGG	35	1.2118471E-7	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content