##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043335_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1082208
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2161248114965	33.0	31.0	34.0	31.0	34.0
2	32.51203465507555	34.0	31.0	34.0	31.0	34.0
3	32.576677496377776	34.0	31.0	34.0	31.0	34.0
4	36.14527983529968	37.0	35.0	37.0	35.0	37.0
5	36.103865430675064	37.0	35.0	37.0	35.0	37.0
6	36.33155548656081	37.0	37.0	37.0	35.0	37.0
7	36.260331655282535	37.0	37.0	37.0	35.0	37.0
8	36.163693116295576	37.0	37.0	37.0	35.0	37.0
9	37.756516307401164	39.0	38.0	39.0	35.0	39.0
10	37.56600209941158	39.0	37.0	39.0	35.0	39.0
11	37.309753762677786	39.0	37.0	39.0	35.0	39.0
12	37.32877598391437	39.0	37.0	39.0	34.0	39.0
13	37.339197270764956	39.0	37.0	39.0	34.0	39.0
14	38.74150440580738	40.0	38.0	41.0	35.0	41.0
15	38.8653558280848	40.0	38.0	41.0	35.0	41.0
16	38.92664164375056	41.0	38.0	41.0	35.0	41.0
17	38.89216121115349	41.0	38.0	41.0	35.0	41.0
18	38.65151985570242	40.0	38.0	41.0	35.0	41.0
19	38.39115401105887	40.0	37.0	41.0	35.0	41.0
20	38.11760955380112	40.0	35.0	41.0	35.0	41.0
21	38.05901360921376	40.0	35.0	41.0	34.0	41.0
22	38.03452109021556	40.0	35.0	41.0	34.0	41.0
23	37.99250328957095	40.0	35.0	41.0	34.0	41.0
24	37.917428997013516	40.0	35.0	41.0	34.0	41.0
25	37.90637012478193	40.0	35.0	41.0	34.0	41.0
26	37.89809907152784	40.0	35.0	41.0	34.0	41.0
27	37.90252520772347	40.0	35.0	41.0	34.0	41.0
28	37.85257547532452	40.0	35.0	41.0	34.0	41.0
29	37.826749571246935	40.0	35.0	41.0	34.0	41.0
30	37.727237277861555	40.0	35.0	41.0	34.0	41.0
31	37.595132359028945	40.0	35.0	41.0	34.0	41.0
32	37.390232746385166	40.0	35.0	41.0	33.0	41.0
33	37.12190262869984	40.0	35.0	41.0	33.0	41.0
34	36.8934068127384	40.0	35.0	41.0	33.0	41.0
35	36.68440909695733	40.0	35.0	41.0	32.0	41.0
36	36.61100269079512	40.0	35.0	41.0	31.0	41.0
37	36.491156043939796	40.0	35.0	41.0	31.0	41.0
38	36.420433964635265	39.0	35.0	41.0	31.0	41.0
39	36.33705720157308	39.0	35.0	41.0	31.0	41.0
40	36.274969321978766	39.0	35.0	41.0	31.0	41.0
41	36.2117374848458	39.0	35.0	41.0	30.0	41.0
42	36.106672654425026	39.0	35.0	41.0	30.0	41.0
43	35.92734483574322	39.0	35.0	41.0	30.0	41.0
44	35.83586057393773	39.0	35.0	41.0	29.0	41.0
45	35.783698697477746	39.0	35.0	41.0	29.0	41.0
46	35.68558354770987	39.0	35.0	41.0	28.0	41.0
47	35.530689109672075	38.0	35.0	40.0	27.0	41.0
48	35.54960876282563	38.0	35.0	40.0	27.0	41.0
49	35.470396633549186	38.0	35.0	40.0	27.0	41.0
50	35.34824913510157	38.0	35.0	40.0	26.0	41.0
51	34.91941382802567	38.0	35.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	3.0
15	17.0
16	33.0
17	63.0
18	112.0
19	254.0
20	506.0
21	881.0
22	1464.0
23	2546.0
24	4780.0
25	9274.0
26	14481.0
27	16643.0
28	15676.0
29	15023.0
30	16060.0
31	19004.0
32	23308.0
33	30716.0
34	55994.0
35	101394.0
36	101841.0
37	83331.0
38	144761.0
39	423873.0
40	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.54832342765901	20.465751500635736	26.402503030840652	27.5834220408646
2	33.3771326768976	19.752764718057897	26.779879653449246	20.09022295159526
3	20.682345722818535	21.87731009196014	38.13989547295899	19.300448712262337
4	22.413066619355984	23.509805878352406	36.1196738519767	17.95745365031491
5	20.198797273721873	29.39074558680032	31.202042490907477	19.20841464857033
6	72.1687512936515	1.3596277240604395	22.245169135692954	4.2264518465951095
7	72.59519426949348	1.4889004701499156	17.984065909695733	7.931839350660871
8	59.014533250539635	3.329027321919631	27.39279325231379	10.263646175226944
9	33.7315931872616	17.822636683521097	29.627483810875543	18.81828631834176
10	26.173988734143528	18.585798663473195	43.315979922528754	11.92423267985452
11	22.880536828410065	15.770720600845678	40.66852213252905	20.680220438215205
12	17.980831780951537	19.521293503651794	44.38730816996363	18.11056654543304
13	13.42422159141311	23.186762618646323	42.875399183890714	20.513616606049855
14	11.509709778526863	22.961112835979776	44.34896064342529	21.180216742068065
15	12.042232177178509	18.342869392944795	48.65996185576155	20.954936574115145
16	12.399742008929891	25.23063958130045	39.398248765486855	22.9713696442828
17	14.258442000059137	22.444576273692306	41.18949407138	22.107487654868567
18	16.17267660191017	28.93316257133564	39.538425145628196	15.355735681125996
19	16.257780389721756	23.2274202371448	36.284984032644374	24.229815340489075
20	13.440392235134096	30.180889440846858	35.297003903131376	21.081714420887668
21	13.960717348236198	24.670026464413493	36.68361350128626	24.685642686064046
22	12.232121736302078	26.73700434666903	31.834822880629233	29.196051036399655
23	11.671416215736716	23.63408882580798	43.24991129246873	21.444583665986574
24	16.716472249327303	21.777144504568437	38.70743886572637	22.798944380377893
25	12.232491351015701	26.378940092847213	38.54508560276768	22.843482953369406
26	14.256131908098999	23.306887400573643	37.47763830982584	24.95934238150152
27	19.79268310712913	23.368890268783822	39.47309574499542	17.365330879091633
28	14.398803187557291	22.92673866761288	36.4003038232946	26.27415432153523
29	17.607058901800762	26.196997250066527	33.805146515272476	22.390797332860227
30	20.824832194920013	21.458074603033797	34.68473713001567	23.032356072030517
31	19.109819923711523	23.091402022531714	35.02866362104143	22.77011443271534
32	20.335647121440612	24.329888524202374	34.390523817972145	20.943940536384872
33	13.3419823176321	20.305153907566755	40.184142050326734	26.16872172447441
34	21.77113826547207	17.689020964546557	39.43936840237736	21.10047236760401
35	20.451244123126052	18.24399745705077	39.67351932345723	21.63123909636595
36	21.670695467045153	18.84046320115911	32.55834368254531	26.930497649250423
37	21.01712424968213	18.01326547207191	40.597371300156716	20.372238978089243
38	18.6745061947426	18.15649117360064	35.670869185960555	27.498133445696205
39	22.86288772583459	17.778375321564802	35.521544841657054	23.837192110943555
40	15.644312368786778	16.57001241905438	41.06631996806529	26.719355244093556
41	18.86910834146486	21.110359561193413	34.266425677873386	25.754106419468346
42	19.675238031875573	16.075929950619475	40.20308480439989	24.04574721310506
43	22.880167213696442	19.321054732546795	35.68620819657589	22.112569857180876
44	17.5781365504598	21.988841331795737	35.75745143262663	24.675570685117833
45	19.828258523315295	19.024254117507912	36.543991543215355	24.603495815961445
46	24.622530973713	16.64384295810048	35.50472737218723	23.22889869599929
47	16.705661018953844	15.140250303084066	41.02926609302463	27.124822584937462
48	18.536270291847778	13.839021703775984	34.18677370708773	33.43793429728851
49	20.98524498063219	13.900377746237321	40.30888701617434	24.80549025695615
50	18.830391200212897	13.466450072444482	40.46486442532304	27.238294302019572
51	16.567240308702207	13.354087199503237	38.364898429876696	31.713774061917853
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	408.0
1	1579.5
2	2751.0
3	35734.0
4	68717.0
5	38536.0
6	8355.0
7	7813.5
8	7272.0
9	7507.5
10	7743.0
11	7831.0
12	7919.0
13	7672.5
14	7426.0
15	6879.5
16	6333.0
17	5734.0
18	5135.0
19	4674.0
20	4213.0
21	3954.5
22	3696.0
23	3421.5
24	3147.0
25	3208.0
26	3679.0
27	4089.0
28	4256.0
29	4423.0
30	5564.5
31	6706.0
32	7719.5
33	8733.0
34	10783.5
35	12834.0
36	15101.5
37	17369.0
38	19456.5
39	21544.0
40	26810.0
41	32076.0
42	41409.0
43	50742.0
44	62045.5
45	73349.0
46	101778.0
47	130207.0
48	160178.5
49	190150.0
50	183489.5
51	176829.0
52	137232.5
53	97636.0
54	73689.0
55	49742.0
56	37329.0
57	24916.0
58	19887.5
59	14859.0
60	12440.5
61	10022.0
62	8400.0
63	6778.0
64	5405.0
65	4032.0
66	3434.0
67	2836.0
68	2223.0
69	1610.0
70	1445.0
71	1280.0
72	1212.5
73	1145.0
74	990.5
75	678.0
76	520.0
77	399.5
78	279.0
79	207.5
80	136.0
81	91.0
82	46.0
83	41.5
84	37.0
85	42.5
86	48.0
87	29.5
88	11.0
89	6.0
90	1.0
91	0.5
92	0.0
93	1.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1082208.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.68674391379135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.27117885029493	20.158513121986413
2	12.623916858707515	7.242781402294717
3	5.55389639902984	4.779696111680909
4	2.829873812757063	3.247194614996248
5	1.6979687823975693	2.4354597817125594
6	1.1293979500189841	1.9439249861373311
7	0.7666577418563337	1.5395040017110742
8	0.5595433235603114	1.2841180825317078
9	0.41151617080768055	1.0624553107509558
>10	3.477181303286249	21.48940158229577
>50	0.4212435691973629	8.189477100791061
>100	0.23745760581168487	12.91673466416017
>500	0.012686091269378556	2.491283833348192
>1k	0.006180403438928014	3.3279326778234295
>5k	9.758531745675813E-4	2.1497564219295695
>10k+	3.252843915225271E-4	5.741766305849912
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61532	5.685783139655223	No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	9053	0.8365305006061681	TruSeq Adapter, Index 14 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	8543	0.7894046246192968	Illumina PCR Primer Index 3 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	5442	0.5028608178834384	TruSeq Adapter, Index 14 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	3902	0.3605591531387682	Illumina PCR Primer Index 3 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	3240	0.29938791803424114	TruSeq Adapter, Index 14 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC	3082	0.28478813684615156	No Hit
ACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	2516	0.23248765486856499	Illumina PCR Primer Index 3 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	2271	0.20984875365918565	TruSeq Adapter, Index 14 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	2045	0.1889655223395133	Illumina PCR Primer Index 3 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	1948	0.18000236553416718	No Hit
TGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCTT	1870	0.17279487861852805	TruSeq Adapter, Index 14 (96% over 27bp)
TCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1831	0.16919113516070847	Illumina PCR Primer Index 3 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1785	0.1649405659540495	Illumina PCR Primer Index 3 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1368	0.12640823205890178	Illumina PCR Primer Index 3 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT	1339	0.12372852538513852	Illumina PCR Primer Index 3 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT	1327	0.12261968124427099	Illumina PCR Primer Index 3 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGC	1323	0.12225006653064845	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTC	1307	0.12077160767615838	No Hit
GGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1260	0.11642863479109378	TruSeq Adapter, Index 14 (95% over 24bp)
ATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	1133	0.10469336763357875	Illumina PCR Primer Index 3 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT	1107	0.10229087199503238	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8099182412253467	0.0
2	0.0	0.0	0.0	2.66806381028416	0.0
3	0.0	0.0	0.0	4.509853928265176	0.0
4	0.0	0.0	0.0	5.723945858836749	0.0
5	0.0	0.0	0.0	11.491506253880955	0.0
6	0.0	0.0	0.0	12.886801797805967	0.0
7	0.0	0.0	0.0	17.671926284041515	0.0
8	0.0	0.0	0.0	23.573009994381856	0.0
9	0.0	0.0	0.0	27.863774801147283	0.0
10	0.0	0.0	0.0	30.607886838759278	0.0
11	0.0	0.0	0.0	33.08291936485408	0.0
12	0.0	0.0	0.0	34.859102871167096	0.0
13	0.0	0.0	0.0	35.580313581123036	0.0
14	0.0	0.0	0.0	36.08945784913806	0.0
15	0.0	0.0	0.0	37.71400691918744	0.0
16	0.0	0.0	0.0	39.61133164789024	0.0
17	0.0	0.0	0.0	41.79649383482658	0.0
18	0.0	0.0	0.0	42.92668322540584	0.0
19	0.0	0.0	0.0	44.10981992371152	0.0
20	0.0	0.0	0.0	45.412249770838876	0.0
21	3.696147136225199E-4	0.0	0.0	46.654617227002575	0.0
22	7.392294272450398E-4	0.0	0.0	47.8614092669801	0.0
23	0.0011088441408675597	0.0	0.0	48.782581537005825	0.0
24	0.003049321387385789	0.0	0.0	49.54297140660575	0.0
25	0.003049321387385789	0.0	0.0	50.23405851740146	0.0
26	0.003049321387385789	0.0	0.0	50.938913776279605	0.0
27	0.003049321387385789	0.0	0.0	51.60588352701144	0.0
28	0.0032341287441970488	0.0	0.0	52.31277166681451	0.0
29	0.0032341287441970488	0.0	0.0	53.13008220231231	0.0
30	0.0032341287441970488	0.0	0.0	53.859609243324755	0.0
31	0.0032341287441970488	0.0	0.0	54.45801546467962	0.0
32	0.0032341287441970488	0.0	0.0	55.04986102486768	0.0
33	0.0032341287441970488	0.0	0.0	55.64032052987965	0.0
34	0.0032341287441970488	0.0	0.0	56.308861143144384	0.0
35	0.0032341287441970488	0.0	0.0	56.87409444395163	0.0
36	0.0032341287441970488	0.0	0.0	57.384347556107514	0.0
37	0.0032341287441970488	0.0	0.0	57.93840001182767	0.0
38	0.0032341287441970488	0.0	0.0	58.44893033501878	0.0
39	0.0032341287441970488	0.0	0.0	58.976555338714924	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGTCG	110	0.0	45.000004	5
TTGACGG	110	0.0	45.000004	1
TCATCGG	55	1.8189894E-12	45.000004	1
TATACGG	75	0.0	45.000004	1
TTACCGG	55	1.8189894E-12	45.000004	1
CGGGTAG	50	2.1827873E-11	45.0	5
GTCGCAA	35	1.2118471E-7	45.0	8
TCCGCGG	45	3.8562575E-10	45.0	1
CACGGTC	25	3.890654E-5	45.0	23
GGTCGAA	20	7.033105E-4	45.0	7
CCTACGG	35	1.2118471E-7	45.0	1
CGATTAC	25	3.890654E-5	45.0	9
TCGATGG	80	0.0	45.0	1
TGACGAT	20	7.033105E-4	45.0	14
AGCGGCA	20	7.033105E-4	45.0	3
TCAATCG	20	7.033105E-4	45.0	41
TACGAGG	195	0.0	45.0	1
CTTGCGG	180	0.0	45.0	1
GGTATCG	25	3.890654E-5	45.0	7
AGTTCGG	35	1.2118471E-7	45.0	1
>>END_MODULE