##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043334_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2725430
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.335362493257946	33.0	31.0	34.0	31.0	34.0
2	32.66748659844502	34.0	31.0	34.0	31.0	34.0
3	32.771920394213026	34.0	31.0	34.0	31.0	34.0
4	36.211575421126206	37.0	37.0	37.0	35.0	37.0
5	36.14814836557901	37.0	37.0	37.0	35.0	37.0
6	36.314045490069454	37.0	37.0	37.0	35.0	37.0
7	36.25753477432919	37.0	37.0	37.0	35.0	37.0
8	36.24020136272075	37.0	37.0	37.0	35.0	37.0
9	37.7993531295979	39.0	38.0	39.0	35.0	39.0
10	37.62769507930859	39.0	37.0	39.0	35.0	39.0
11	37.320602987418496	39.0	37.0	39.0	35.0	39.0
12	37.326645336699166	39.0	37.0	39.0	35.0	39.0
13	37.31399192054098	39.0	37.0	39.0	35.0	39.0
14	38.67043805931541	40.0	38.0	41.0	35.0	41.0
15	38.70066264772898	41.0	38.0	41.0	35.0	41.0
16	38.57239554859233	41.0	38.0	41.0	34.0	41.0
17	38.55940236953435	40.0	38.0	41.0	34.0	41.0
18	38.32819408313551	40.0	37.0	41.0	34.0	41.0
19	38.06686504514884	40.0	37.0	41.0	34.0	41.0
20	37.78126864384703	40.0	35.0	41.0	34.0	41.0
21	37.70158360332131	40.0	35.0	41.0	34.0	41.0
22	37.64302843954899	39.0	35.0	41.0	34.0	41.0
23	37.652470986229694	39.0	35.0	41.0	34.0	41.0
24	37.56445698476938	39.0	35.0	41.0	34.0	41.0
25	37.45735645384398	39.0	35.0	41.0	34.0	41.0
26	37.35993879864829	39.0	35.0	41.0	33.0	41.0
27	37.37228327273127	39.0	35.0	41.0	34.0	41.0
28	37.33653405150747	39.0	35.0	41.0	33.0	41.0
29	37.26370260839574	39.0	35.0	41.0	33.0	41.0
30	37.156331294511325	39.0	35.0	41.0	33.0	41.0
31	37.056041798908794	39.0	35.0	41.0	33.0	41.0
32	36.77644628554026	39.0	35.0	41.0	32.0	41.0
33	36.39199062166337	39.0	35.0	41.0	31.0	41.0
34	36.10635165827044	39.0	35.0	41.0	30.0	41.0
35	35.949168388107566	39.0	35.0	41.0	30.0	41.0
36	35.914677683888414	39.0	35.0	41.0	30.0	41.0
37	35.82913338445677	39.0	35.0	41.0	30.0	41.0
38	35.72973292287823	38.0	35.0	41.0	29.0	41.0
39	35.667356343769605	38.0	35.0	41.0	29.0	41.0
40	35.55535860396341	38.0	35.0	41.0	28.0	41.0
41	35.490110551362534	38.0	35.0	41.0	28.0	41.0
42	35.40038379264924	38.0	35.0	40.0	27.0	41.0
43	35.30174174350469	38.0	35.0	40.0	27.0	41.0
44	35.143772909229	37.0	35.0	40.0	26.0	41.0
45	35.053120425033846	37.0	35.0	40.0	26.0	41.0
46	35.00518780522707	37.0	35.0	40.0	25.0	41.0
47	34.89978865720272	37.0	35.0	40.0	25.0	41.0
48	34.780367868556525	36.0	35.0	40.0	25.0	41.0
49	34.71412804584965	36.0	35.0	40.0	26.0	41.0
50	34.54740132749694	36.0	35.0	40.0	25.0	41.0
51	34.316506019233664	36.0	34.0	39.0	24.0	41.0
52	33.87021570908077	35.0	34.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	6.0
12	4.0
13	10.0
14	28.0
15	65.0
16	128.0
17	266.0
18	599.0
19	1156.0
20	2058.0
21	3513.0
22	5451.0
23	8722.0
24	14985.0
25	27151.0
26	40944.0
27	48248.0
28	49387.0
29	49051.0
30	51005.0
31	56609.0
32	66013.0
33	84360.0
34	187998.0
35	255161.0
36	260150.0
37	243690.0
38	389323.0
39	877810.0
40	1536.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.835743350590548	16.825381682890406	22.776222467647305	31.562652498871735
2	36.72539746021729	18.99329647064867	23.962457300315915	20.318848768818132
3	25.53266823950716	20.441178089329025	35.45191034075357	18.574243330410248
4	22.153238204613583	23.000554041013714	34.00938567492102	20.836822079451682
5	21.186161449752884	30.5822934362651	30.59429154298588	17.637253570996137
6	75.87837515548004	2.0068759792032815	19.16662691758732	2.948121947729349
7	75.98742216824502	1.8621648693967559	17.546368829872716	4.604044132485516
8	67.11234557482672	3.243341417684549	22.781726186326562	6.862586821162166
9	39.17304792271311	15.382857017057857	27.61178969924012	17.832305360988908
10	27.669872277035186	16.492626851542692	36.66782856283229	19.16967230858984
11	24.827898716899718	13.506639319300074	40.578220684442456	21.08724127935775
12	20.144417578143635	14.739545686368757	42.93902246617965	22.177014269307964
13	16.40295292852871	18.111050366364207	44.299358266402	21.186638438705085
14	16.924925608069184	18.07065307125848	43.624712430699006	21.379708889973326
15	20.197363351838057	14.753341674524753	43.60798112591407	21.441313847723112
16	18.962145422924088	19.104948576921807	39.743159794968136	22.18974620518597
17	19.967417985418816	19.92441559680491	39.53126662581684	20.576899791959434
18	20.21409465662299	25.279974169213666	36.247601295942296	18.25832987822105
19	21.16895315601575	21.26768986912157	35.408907952139664	22.154449022723018
20	20.41167815720822	25.382563485394964	35.492931390642944	18.71282696675387
21	18.179149712155514	19.98312192938362	37.25573579215023	24.581992566310635
22	19.34274591532345	21.638053444777523	35.06033910245356	23.95886153744547
23	20.552022983529206	19.549722429121278	38.30679929405635	21.59145529329317
24	22.580730380160194	18.35504856114448	35.21635118128149	23.84786987741384
25	18.255761476170733	19.28682813354223	37.6576907130251	24.799719677261937
26	17.854063395500894	18.472057620265424	37.72421232612835	25.949666658105325
27	21.272092844064975	20.52765985550904	37.3988691692687	20.801378131157286
28	21.689348102868173	21.447184480980983	33.99947898129837	22.86398843485248
29	22.67477058665972	19.371438635371298	36.12505182668423	21.828738951284752
30	24.463589231790948	18.85654740719813	34.944320712694875	21.735542648316045
31	26.18218042657489	20.12309984112599	31.48200467449173	22.21271505780739
32	25.266801935841315	18.320118293260144	31.9501509853491	24.462928785549437
33	21.912578932498725	20.170431821767572	36.80813669769541	21.10885254803829
34	20.52655911177319	21.419739270500436	34.048828992122345	24.004872625604033
35	23.09921737120381	23.69952631327901	32.315414448362276	20.88584186715491
36	23.679823000407275	21.599380648191293	31.262809905225964	23.457986446175465
37	22.800805744414642	23.367285162341357	32.19935936714574	21.632549726098265
38	23.842109318529552	20.250088976785317	29.38912391806064	26.518677786624494
39	26.45494472431873	18.094282370121412	33.326117346620535	22.124655558939324
40	21.93936369673776	18.212355481520348	35.90119724227003	23.947083579471865
41	21.9223755517478	22.22691465199987	31.009785611811715	24.84092418444062
42	20.939741618753736	17.88682886737139	34.05290174394499	27.12052776992988
43	22.854742187471334	19.342562458034145	34.01672396649336	23.78597138800116
44	21.71785736562671	19.95453928370936	34.196952407510004	24.130650943153924
45	22.694804122652204	19.938505116623798	33.640049460085194	23.7266413006388
46	23.227894313924775	19.306458063498237	32.89660714089153	24.56904048168546
47	20.413769570306336	20.234862021772713	35.11933896669516	24.232029441225787
48	21.567092165273003	18.70270012438404	32.8960567690236	26.83415094131935
49	21.33777055363741	16.798340078446337	36.41289631360923	25.450993054307027
50	20.06927347244288	17.06703162436753	37.840854470670685	25.022840432518905
51	19.10979184935955	15.921781150130437	36.95082977731954	28.01759722319047
52	17.74109039674473	17.01551681752971	40.02957331503653	25.21381947068903
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1006.0
1	4878.0
2	8750.0
3	99126.0
4	189502.0
5	107483.5
6	25465.0
7	23209.0
8	20953.0
9	20217.0
10	19481.0
11	19057.5
12	18634.0
13	17676.0
14	15724.0
15	14730.0
16	14229.5
17	13729.0
18	12484.0
19	11239.0
20	10507.0
21	9775.0
22	9738.0
23	9701.0
24	9523.5
25	9346.0
26	10444.0
27	11542.0
28	12393.0
29	13244.0
30	14873.5
31	16503.0
32	19304.0
33	22105.0
34	25758.5
35	29412.0
36	32714.0
37	36016.0
38	38551.0
39	44799.0
40	48512.0
41	54781.5
42	61051.0
43	72484.0
44	83917.0
45	98828.0
46	113739.0
47	135377.0
48	157015.0
49	203648.5
50	250282.0
51	270250.5
52	290219.0
53	267540.0
54	244861.0
55	218065.5
56	191270.0
57	169384.5
58	147499.0
59	140380.0
60	133261.0
61	126559.5
62	119858.0
63	114861.0
64	91690.5
65	73517.0
66	61498.0
67	49479.0
68	41022.5
69	32566.0
70	28420.5
71	24275.0
72	24599.0
73	24923.0
74	19842.5
75	14762.0
76	11112.5
77	7463.0
78	5970.0
79	4477.0
80	3172.5
81	1868.0
82	1363.5
83	859.0
84	674.0
85	489.0
86	398.0
87	307.0
88	180.0
89	45.5
90	38.0
91	46.5
92	55.0
93	33.0
94	11.0
95	7.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2725430.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.117384072859764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.38266046828278	21.658284932161127
2	10.601724296579203	6.173889563561313
3	3.9385118303946904	3.4403748492331236
4	1.9091103014150468	2.2235319153501996
5	1.1599581920567563	1.6887474093288304
6	0.7669547312750422	1.339902928621941
7	0.6001165537135925	1.2231676928071753
8	0.49800813215912276	1.1600555244387745
9	0.41708711693581096	1.0930037198095601
>10	5.190666372517253	33.330577702512706
>50	0.37559554405744755	7.1635201787203435
>100	0.14783802016526826	7.762694039317318
>500	0.0059474914091896475	1.1675337520477562
>1k	0.004682067705106743	2.62931828655688
>5k	6.327118520414517E-4	1.1985947850573895
>10k+	5.061694816331613E-4	6.74680272047567
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	143985	5.283019560216186	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	17205	0.6312765325104662	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	11114	0.4077888626748806	No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	10805	0.39645120219561686	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCTT	9217	0.3381851671112448	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	6895	0.25298760195638853	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT	5700	0.20914130981166276	No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	5680	0.20840748065442885	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC	5038	0.1848515647072205	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCG	4236	0.15542501550214094	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4195	0.15392066572981145	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3957	0.14518809875872798	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT	3816	0.14001460320022896	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG	3046	0.11176218064672364	No Hit
GGGCCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT	2900	0.10640522779891615	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCT	2754	0.10104827495110863	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.35165093214648696	0.0
2	0.0	0.0	0.0	1.1196765281074912	0.0
3	0.0	0.0	0.0	1.8481487324935881	0.0
4	0.0	0.0	0.0	2.3268988746729873	0.0
5	0.0	0.0	0.0	4.752094164957456	0.0
6	0.0	0.0	0.0	5.678590167423122	0.0
7	0.0	0.0	0.0	8.037153770230752	0.0
8	0.0	0.0	0.0	10.777565375005045	0.0
9	0.0	0.0	0.0	13.388859739564031	0.0
10	0.0	0.0	0.0	14.898896687862099	0.0
11	0.0	0.0	0.0	16.140132015865387	0.0
12	0.0	0.0	0.0	17.02483644782658	0.0
13	0.0	0.0	0.0	17.472729073944294	0.0
14	3.669145786169522E-5	0.0	0.0	17.764022557908294	0.0
15	3.669145786169522E-5	0.0	0.0	18.520527036100724	0.0
16	3.669145786169522E-5	0.0	0.0	19.361201718627886	0.0
17	3.669145786169522E-5	0.0	0.0	20.40063402839185	0.0
18	3.669145786169522E-5	0.0	0.0	21.00208040566076	0.0
19	3.669145786169522E-5	0.0	0.0	21.675772263459343	0.0
20	3.669145786169522E-5	0.0	0.0	22.41638934039766	0.0
21	7.338291572339044E-4	0.0	0.0	23.144164407084386	0.0
22	7.338291572339044E-4	0.0	0.0	23.871645942108216	0.0
23	7.338291572339044E-4	0.0	0.0	24.488319274389728	0.0
24	7.705206150955996E-4	0.0	0.0	25.006365967939004	0.0
25	7.705206150955996E-4	0.0	0.0	25.504232359664346	0.0
26	7.705206150955996E-4	0.0	0.0	25.996191426673956	0.0
27	7.705206150955996E-4	0.0	0.0	26.528144182752815	0.0
28	7.705206150955996E-4	0.0	0.0	27.03345894042408	0.0
29	7.705206150955996E-4	0.0	0.0	27.592930289899208	0.0
30	7.705206150955996E-4	0.0	0.0	28.091310362034616	0.0
31	7.705206150955996E-4	0.0	0.0	28.586681734625362	0.0
32	8.4390353081899E-4	0.0	0.0	29.149125092187287	0.0
33	8.4390353081899E-4	0.0	0.0	29.68030732765105	0.0
34	8.4390353081899E-4	0.0	0.0	30.27379165856397	0.0
35	8.4390353081899E-4	0.0	0.0	30.774593366918246	0.0
36	8.4390353081899E-4	0.0	0.0	31.286255746799586	0.0
37	8.4390353081899E-4	0.0	0.0	31.913202687282375	0.0
38	8.805949886806852E-4	0.0	0.0	32.55622048630858	0.0
39	8.805949886806852E-4	0.0	0.0	33.261173466205335	0.0
40	9.172864465423805E-4	0.0	0.0	33.911162642225264	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	34550	0.0	45.37424	1
CGGTCTA	445	0.0	43.932583	30
CACTTAC	4200	0.0	42.33095	36
ACGTAGG	175	0.0	42.057144	1
TATACGG	115	0.0	42.0	1
ACCCGCA	4275	0.0	41.911106	31
GCACTTA	4255	0.0	41.783783	35
CCGCACT	4255	0.0	41.72973	33
CGCACTT	4290	0.0	41.38928	34
GTTTTTT	38815	0.0	41.324745	2
CCCGCAC	4365	0.0	41.31042	32
AAGCTTA	4825	0.0	40.947147	22
TCAACGC	4955	0.0	40.940464	15
TAGTAGG	495	0.0	40.888885	1
GTTGATC	1870	0.0	40.834225	15
GGGCGAT	2355	0.0	40.823776	6
AACGCAA	4930	0.0	40.821503	17
CTTATGA	4805	0.0	40.782516	25
CGCAAGC	4945	0.0	40.744186	19
GACGGTC	480	0.0	40.729168	28
>>END_MODULE