FastQCFastQC Report
Thu 26 May 2016
SRR1043333_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043333_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2650149
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1362735.142088237302884No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC164500.6207198161310931No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC104130.3929213036700956No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG92530.34915017985781177No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT89570.33798099654019453Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG67980.2565138790309526No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC56860.21455397413503918No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC54290.20485640618697287No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT47450.1790465366286952No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC40870.1542177439834515No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCC38810.1464445961340287No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC37960.14323722930295615No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCT35310.13323779153549478No Hit
GGGCCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC29680.1119937029955674No Hit
CGTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT29610.11172956690359674No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGATA253.8921888E-545.00000437
CGTTTTT334650.044.4083371
CACTTAC40300.041.5942936
CGCACTT40500.041.534
GCACTTA40450.041.49567435
ACCCGCA41200.041.28640731
CGGTCTA3950.041.0126630
CCGCACT41050.040.94397433
CATGCGG4250.040.7647061
TCAACGC47300.040.67124615
CCCGCAC42150.040.62277632
TCGGCGT14800.040.5912174
AAGCTTA45350.040.5347322
CAACGCA46850.040.5336216
CTTATGA44900.040.48997525
GCTTATG45250.040.4751424
ATCAACG47550.040.45741314
AGCTTAT45450.040.3465423
CGCAAGC47100.040.31847419
AACGCAA47000.040.3085117