##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043333_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2650149
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29888621356761	33.0	31.0	34.0	31.0	34.0
2	32.60348040808272	34.0	31.0	34.0	31.0	34.0
3	32.688029239110705	34.0	31.0	34.0	31.0	34.0
4	36.1838606055735	37.0	37.0	37.0	35.0	37.0
5	36.16408171766946	37.0	37.0	37.0	35.0	37.0
6	36.33223905523803	37.0	37.0	37.0	35.0	37.0
7	36.28842906568649	37.0	37.0	37.0	35.0	37.0
8	36.196605549348355	37.0	37.0	37.0	35.0	37.0
9	37.76447588418613	39.0	38.0	39.0	35.0	39.0
10	37.574155641814855	39.0	37.0	39.0	35.0	39.0
11	37.310673852677716	39.0	37.0	39.0	35.0	39.0
12	37.326455984172966	39.0	37.0	39.0	35.0	39.0
13	37.32449156632325	39.0	37.0	39.0	35.0	39.0
14	38.67956745073579	40.0	38.0	41.0	35.0	41.0
15	38.72368308347946	40.0	38.0	41.0	35.0	41.0
16	38.6334157060603	40.0	38.0	41.0	34.0	41.0
17	38.592668940501085	40.0	38.0	41.0	34.0	41.0
18	38.384037652222574	40.0	38.0	41.0	34.0	41.0
19	38.14470129792702	40.0	37.0	41.0	34.0	41.0
20	37.879389045672525	40.0	35.0	41.0	34.0	41.0
21	37.802781277581	40.0	35.0	41.0	34.0	41.0
22	37.75811926046422	40.0	35.0	41.0	34.0	41.0
23	37.73337121799567	39.0	35.0	41.0	34.0	41.0
24	37.64498713091226	39.0	35.0	41.0	34.0	41.0
25	37.60667532278374	39.0	35.0	41.0	34.0	41.0
26	37.561296742183174	39.0	35.0	41.0	34.0	41.0
27	37.527920882939036	39.0	35.0	41.0	34.0	41.0
28	37.431880999898496	39.0	35.0	41.0	34.0	41.0
29	37.39957149579137	39.0	35.0	41.0	34.0	41.0
30	37.25425174207186	39.0	35.0	41.0	33.0	41.0
31	37.138339014146	39.0	35.0	41.0	33.0	41.0
32	36.876666557238856	39.0	35.0	41.0	33.0	41.0
33	36.48569193656659	39.0	35.0	41.0	31.0	41.0
34	36.16351533442082	39.0	35.0	41.0	30.0	41.0
35	35.994798783011824	39.0	35.0	41.0	30.0	41.0
36	35.938949093050994	39.0	35.0	41.0	30.0	41.0
37	35.81179812908633	38.0	35.0	41.0	30.0	41.0
38	35.71153470993518	38.0	35.0	41.0	29.0	41.0
39	35.60541652563686	38.0	35.0	41.0	29.0	41.0
40	35.472799453917496	38.0	35.0	40.0	28.0	41.0
41	35.36549378921713	38.0	35.0	40.0	27.0	41.0
42	35.25855565102189	38.0	35.0	40.0	27.0	41.0
43	35.14404510840711	37.0	35.0	40.0	26.0	41.0
44	35.02979228715065	37.0	35.0	40.0	26.0	41.0
45	34.97704959230594	37.0	35.0	40.0	25.0	41.0
46	34.8706065206145	37.0	35.0	40.0	25.0	41.0
47	34.70710401566101	36.0	35.0	40.0	24.0	41.0
48	34.624038497458066	36.0	35.0	40.0	24.0	41.0
49	34.5427592939114	36.0	34.0	40.0	25.0	41.0
50	34.40831402309832	36.0	34.0	40.0	24.0	41.0
51	33.87342900342585	35.0	34.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	7.0
13	5.0
14	29.0
15	52.0
16	118.0
17	247.0
18	483.0
19	1069.0
20	1938.0
21	3161.0
22	4803.0
23	7463.0
24	12637.0
25	23220.0
26	37145.0
27	46579.0
28	49075.0
29	48375.0
30	50291.0
31	54642.0
32	62736.0
33	81168.0
34	180728.0
35	252702.0
36	246070.0
37	239368.0
38	398278.0
39	847620.0
40	137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.044291471913464	16.955688151873723	22.83384821004404	31.16617216616877
2	36.44519610029474	19.072361591744464	24.01400826896903	20.46843403899177
3	25.589202720299873	20.513412642081633	35.16255123768512	18.73483339993336
4	22.337272357139167	23.162358041000715	33.56113184579433	20.93923775606579
5	21.359516012118565	30.71517865599255	30.29939071350328	17.625914618385607
6	76.31246394070673	1.9854355358887368	18.765246784237412	2.9368537391671183
7	76.36917773302557	1.8312932593601343	17.165789546172686	4.6337394614416025
8	67.45590530947506	3.1921224051930666	22.433795231890734	6.918177053441147
9	39.414010306590306	15.328496624152077	27.28891847213119	17.968574597126423
10	27.972238542059337	16.509373623898128	36.311618705212425	19.206769128830114
11	25.059307986079272	13.505127447551063	40.281697368713985	21.15386719765568
12	20.331045537439593	14.716380097873744	42.70091983507343	22.25165452961324
13	16.535787233095196	18.107774317594973	44.05235328277769	21.304085166532147
14	17.081039594377522	18.054947099200838	43.38446630736612	21.479546999055525
15	20.334252904270667	14.738529795871855	43.37341032523077	21.55380697462671
16	19.07583309466751	19.012553633776818	39.65188372427362	22.25972954728206
17	20.187695106954365	19.81967806338436	39.35325900543705	20.639367824224223
18	20.377910826900674	25.35219717834733	36.084084328843396	18.185807665908595
19	21.350573118718984	21.286161646005564	35.14436358106657	22.218901654208874
20	20.57457901423656	25.476643011392948	35.1578722554845	18.790905718885995
21	18.244370410871237	20.059777770985708	36.978600071165815	24.717251746977244
22	19.397550854687793	21.717043079464588	34.702690301564175	24.18271576428344
23	20.64408454015227	19.586559095356527	38.01706998361224	21.752286380878964
24	22.677290974960275	18.34100648680508	35.00361677777363	23.978085760461017
25	18.291273434059747	19.225183187813215	37.50645718410549	24.977086194021545
26	17.980875792266772	18.371495338563985	37.4804586459101	26.167170223259145
27	21.383778798852443	20.53043055315003	37.192512571934635	20.89327807606289
28	21.735155268628294	21.487735217906614	33.78727007424865	22.989839439216436
29	22.765512429678484	19.400154481880076	35.863568425775306	21.970764662666138
30	24.614125469926407	18.793320677441155	34.65099509499277	21.941558757639665
31	26.379233771384175	20.11249178819757	31.235979561903875	22.27229487851438
32	25.50750920042609	18.129810814410813	31.703311776054854	24.659368209108244
33	22.127887903661268	20.08758752809748	36.62345022864752	21.161074339593736
34	20.64717870580107	21.36317618367873	33.924318972254014	24.065326138266187
35	23.120888674561318	23.662707266648024	32.18954858764545	21.026855471145208
36	23.607314154789034	21.571768228880718	31.150022130831132	23.670895485499116
37	22.950369960330534	23.234504927836134	32.073932446817146	21.741192665016193
38	24.090720936822798	20.131811456638854	29.172774813793488	26.60469279274486
39	26.626993425652675	18.03174085683484	33.111760885897354	22.229504831615127
40	22.18313008061056	18.07434223509697	35.71851997755598	24.024007706736487
41	22.146188761462092	22.092682335974317	30.80539999826425	24.95572890429934
42	21.146094049806255	17.787301770579692	33.89511306722754	27.17149111238651
43	23.04202518424436	19.15662100508311	33.87507645796519	23.92627735270734
44	21.799830877433685	19.795490744105333	34.20800113503052	24.196677243430464
45	22.85743178968428	19.728211508107655	33.558905555876294	23.85545114633177
46	23.438870795566586	19.185525040290187	32.813287101970495	24.56231706217273
47	20.684270959859237	19.982084026218903	35.00410731623014	24.329537697691713
48	21.715005458183672	18.554315247935115	32.73110304364019	26.999576250241024
49	21.52961965534768	16.626838717370234	36.27886582980806	25.564675797474028
50	20.238522437795005	16.919199637454348	37.777083477193166	25.065194447557477
51	19.254011755565443	15.826317690061956	36.71684120402287	28.202829350349734
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	881.0
1	4711.0
2	8541.0
3	93905.0
4	179269.0
5	102660.0
6	26051.0
7	23130.5
8	20210.0
9	19507.5
10	18805.0
11	18313.0
12	17821.0
13	16828.5
14	15836.0
15	14959.0
16	14082.0
17	13243.5
18	12405.0
19	11176.5
20	9948.0
21	9512.0
22	9076.0
23	8960.5
24	8845.0
25	8753.5
26	10079.5
27	11497.0
28	12310.5
29	13124.0
30	15361.0
31	17598.0
32	20077.0
33	22556.0
34	26858.5
35	31161.0
36	34012.5
37	36864.0
38	40433.5
39	44003.0
40	48403.5
41	52804.0
42	61621.0
43	70438.0
44	80579.0
45	90720.0
46	103974.5
47	117229.0
48	156450.5
49	195672.0
50	235592.5
51	275513.0
52	280535.5
53	285558.0
54	251928.5
55	218299.0
56	192625.0
57	166951.0
58	149868.0
59	132785.0
60	128714.0
61	124643.0
62	117015.0
63	109387.0
64	102473.5
65	95560.0
66	78113.5
67	60667.0
68	49772.0
69	38877.0
70	33003.0
71	27129.0
72	25727.5
73	24326.0
74	20980.0
75	13306.5
76	8979.0
77	7043.0
78	5107.0
79	3781.5
80	2456.0
81	1832.5
82	1209.0
83	814.0
84	419.0
85	406.5
86	394.0
87	232.5
88	71.0
89	57.5
90	44.0
91	39.5
92	35.0
93	19.0
94	3.0
95	4.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2650149.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.883552850845877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.81949410706001	21.321692594639735
2	10.812396457865608	6.246008490701201
3	3.933575260687309	3.408468868045252
4	1.9978215012164366	2.308167316677648
5	1.1597726894538771	1.6749177885404365
6	0.8124285160585942	1.4079493188667616
7	0.6223683398919487	1.258334618657362
8	0.5369505386096582	1.240723140817778
9	0.441932235746597	1.1488115778911383
>10	5.342690107925213	33.82180509119365
>50	0.3593067640381393	6.836512015334348
>100	0.14952988880601523	7.837976011052057
>500	0.006064553499680027	1.2168314751031906
>1k	0.004614334184539151	2.684112038353891
>5k	6.591905977913073E-4	1.3755469683978265
>10k+	3.9551435867478434E-4	6.21214268572787
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	136273	5.142088237302884	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	16450	0.6207198161310931	No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	10413	0.3929213036700956	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	9253	0.34915017985781177	No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	8957	0.33798099654019453	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG	6798	0.2565138790309526	No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	5686	0.21455397413503918	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC	5429	0.20485640618697287	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT	4745	0.1790465366286952	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4087	0.1542177439834515	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCC	3881	0.1464445961340287	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3796	0.14323722930295615	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCT	3531	0.13323779153549478	No Hit
GGGCCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC	2968	0.1119937029955674	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT	2961	0.11172956690359674	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3501312567708457	0.0
2	0.0	0.0	0.0	1.114880710480807	0.0
3	0.0	0.0	0.0	1.8537070934502173	0.0
4	0.0	0.0	0.0	2.339642035221416	0.0
5	0.0	0.0	0.0	4.761203992681166	0.0
6	0.0	0.0	0.0	5.683906829389593	0.0
7	0.0	0.0	0.0	8.086564189409728	0.0
8	0.0	0.0	0.0	10.891161213954385	0.0
9	0.0	0.0	0.0	13.589273659707436	0.0
10	0.0	0.0	0.0	15.14118640121744	0.0
11	0.0	0.0	0.0	16.410737660410792	0.0
12	0.0	0.0	0.0	17.32234678125645	0.0
13	0.0	0.0	0.0	17.78745270548939	0.0
14	0.0	0.0	0.0	18.092492157988097	0.0
15	0.0	0.0	0.0	18.879089439876775	0.0
16	0.0	0.0	0.0	19.747568910276364	0.0
17	0.0	0.0	0.0	20.80649050298681	0.0
18	0.0	0.0	0.0	21.437964431433855	0.0
19	0.0	0.0	0.0	22.12569934747065	0.0
20	0.0	0.0	0.0	22.87924188413557	0.0
21	4.150710016682081E-4	0.0	0.0	23.619388947564836	0.0
22	4.905384565169732E-4	0.0	0.0	24.374252164689608	0.0
23	4.905384565169732E-4	0.0	0.0	25.0118389569794	0.0
24	4.905384565169732E-4	0.0	0.0	25.551393525420647	0.0
25	4.905384565169732E-4	0.0	0.0	26.061440319016025	0.0
26	4.905384565169732E-4	0.0	0.0	26.56273288784895	0.0
27	4.905384565169732E-4	0.0	0.0	27.10896632604431	0.0
28	4.905384565169732E-4	0.0	0.0	27.626295729032595	0.0
29	4.905384565169732E-4	0.0	0.0	28.184754894913457	0.0
30	5.282721839413557E-4	0.0	0.0	28.68812281875472	0.0
31	5.282721839413557E-4	0.0	0.0	29.194018902333415	0.0
32	5.282721839413557E-4	0.0	0.0	29.763609517804472	0.0
33	5.282721839413557E-4	0.0	0.0	30.29535320466887	0.0
34	5.282721839413557E-4	0.0	0.0	30.889206606873802	0.0
35	5.282721839413557E-4	0.0	0.0	31.387744613604745	0.0
36	5.282721839413557E-4	0.0	0.0	31.895640584736935	0.0
37	5.282721839413557E-4	0.0	0.0	32.52413354871745	0.0
38	5.660059113657383E-4	0.0	0.0	33.16066379663936	0.0
39	5.660059113657383E-4	0.0	0.0	33.88492496082296	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGATA	25	3.8921888E-5	45.000004	37
CGTTTTT	33465	0.0	44.408337	1
CACTTAC	4030	0.0	41.59429	36
CGCACTT	4050	0.0	41.5	34
GCACTTA	4045	0.0	41.495674	35
ACCCGCA	4120	0.0	41.286407	31
CGGTCTA	395	0.0	41.01266	30
CCGCACT	4105	0.0	40.943974	33
CATGCGG	425	0.0	40.764706	1
TCAACGC	4730	0.0	40.671246	15
CCCGCAC	4215	0.0	40.622776	32
TCGGCGT	1480	0.0	40.591217	4
AAGCTTA	4535	0.0	40.53473	22
CAACGCA	4685	0.0	40.53362	16
CTTATGA	4490	0.0	40.489975	25
GCTTATG	4525	0.0	40.47514	24
ATCAACG	4755	0.0	40.457413	14
AGCTTAT	4545	0.0	40.34654	23
CGCAAGC	4710	0.0	40.318474	19
AACGCAA	4700	0.0	40.30851	17
>>END_MODULE