FastQCFastQC Report
Thu 26 May 2016
SRR1043332_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043332_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1159473
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT817197.047943332876229No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTT81010.6986794862838548Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT80740.6963508421498388No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT65800.5674992000676169No Hit
CGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC39780.3430869024116991No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCG31440.2711576724943142No Hit
TCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT31350.27038145778297556No Hit
ACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC23620.2037132386868862No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT22730.19603733765253695No Hit
GCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC21350.1841353787453438No Hit
AGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC18400.15869278542924242No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC16840.14523839709937186No Hit
TCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC16190.1396324019619258No Hit
CGTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG16120.13902867940866237No Hit
ACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT15480.13350893035025396No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCT15440.13316394603410342No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTT15350.13238773132276474No Hit
TGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTTG12750.10996375077298048Illumina Single End Adapter 1 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC12430.10720387624377627No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCC12300.10608267721628704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA351.01957994E-746.00000437
CTCGCGG700.046.0000041
ACCGTAG351.01957994E-746.00000438
GCGTAGG1450.046.0000041
GCGAGAC253.417174E-546.020
CGGGTAC1100.046.05
AACCGGC206.3114776E-446.02
ACACGTT253.417174E-546.022
TGCCCGC206.3114776E-446.028
ACGTTTC253.417174E-546.024
TGGACCG206.3114776E-446.022
GCAACCG405.6115823E-946.035
CAACCGG253.417174E-546.01
TCGGGCA405.6115823E-946.04
GACCATA600.046.08
CCCGTGA301.8614173E-646.013
ACTAGGT405.6115823E-946.034
TTGCGGA301.8614173E-646.02
AGCGATG206.3114776E-446.016
CGCAGCG206.3114776E-446.025
Produced by FastQC (version 0.11.5)