##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043332_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1159473
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17298721056894	33.0	31.0	34.0	30.0	34.0
2	32.5024196337474	34.0	31.0	34.0	31.0	34.0
3	32.573150043166166	34.0	31.0	34.0	31.0	34.0
4	36.11540501589946	37.0	35.0	37.0	35.0	37.0
5	35.99940231467227	37.0	35.0	37.0	35.0	37.0
6	36.26424677418103	37.0	37.0	37.0	35.0	37.0
7	36.1917302084654	37.0	37.0	37.0	35.0	37.0
8	36.15868847312529	37.0	37.0	37.0	35.0	37.0
9	37.681722644684264	39.0	38.0	39.0	35.0	39.0
10	37.52459091328561	39.0	37.0	39.0	35.0	39.0
11	37.24236183162523	39.0	37.0	39.0	34.0	39.0
12	37.260804693166634	39.0	37.0	39.0	34.0	39.0
13	37.256820986775885	39.0	37.0	39.0	34.0	39.0
14	38.64747087685526	40.0	38.0	41.0	34.0	41.0
15	38.75419091259564	40.0	38.0	41.0	35.0	41.0
16	38.77103822167485	40.0	38.0	41.0	35.0	41.0
17	38.7414954897613	40.0	38.0	41.0	35.0	41.0
18	38.454296046565986	40.0	38.0	41.0	34.0	41.0
19	38.16027626344037	40.0	37.0	41.0	34.0	41.0
20	37.84852946122937	40.0	35.0	41.0	34.0	41.0
21	37.77787581082095	40.0	35.0	41.0	34.0	41.0
22	37.73887705880171	40.0	35.0	41.0	34.0	41.0
23	37.7266723761571	39.0	35.0	41.0	34.0	41.0
24	37.6380036447593	39.0	35.0	41.0	34.0	41.0
25	37.551559199739884	39.0	35.0	41.0	34.0	41.0
26	37.50857156656515	39.0	35.0	41.0	33.0	41.0
27	37.542831959002065	39.0	35.0	41.0	34.0	41.0
28	37.523255823982105	39.0	35.0	41.0	33.0	41.0
29	37.481922390603316	40.0	35.0	41.0	33.0	41.0
30	37.42176575047457	40.0	35.0	41.0	33.0	41.0
31	37.31959950770738	40.0	35.0	41.0	33.0	41.0
32	37.11156189061755	40.0	35.0	41.0	33.0	41.0
33	36.920874397247715	40.0	35.0	41.0	32.0	41.0
34	36.680369443704166	40.0	35.0	41.0	31.0	41.0
35	36.540320473180486	40.0	35.0	41.0	31.0	41.0
36	36.48780523565448	40.0	35.0	41.0	31.0	41.0
37	36.35564001921563	39.0	35.0	41.0	31.0	41.0
38	36.3027228749613	39.0	35.0	41.0	30.0	41.0
39	36.270916183473005	39.0	35.0	41.0	30.0	41.0
40	36.21118732389629	39.0	35.0	41.0	30.0	41.0
41	36.21122095986711	39.0	35.0	41.0	30.0	41.0
42	36.160415982088416	39.0	35.0	41.0	30.0	41.0
43	36.0602325366783	39.0	35.0	41.0	30.0	41.0
44	35.92347989129544	39.0	35.0	41.0	30.0	41.0
45	35.856053569164615	39.0	35.0	41.0	29.0	41.0
46	35.83587457405218	39.0	35.0	41.0	29.0	41.0
47	35.74352141015789	39.0	35.0	41.0	29.0	41.0
48	35.70689270039061	38.0	35.0	41.0	28.0	41.0
49	35.656490491801016	38.0	35.0	41.0	28.0	41.0
50	35.51752649695163	38.0	35.0	40.0	27.0	41.0
51	35.44194302066542	38.0	35.0	40.0	27.0	41.0
52	35.10371263496433	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	1.0
14	6.0
15	10.0
16	49.0
17	108.0
18	228.0
19	424.0
20	794.0
21	1319.0
22	2068.0
23	3506.0
24	6468.0
25	11590.0
26	15959.0
27	17707.0
28	16506.0
29	16327.0
30	18180.0
31	21528.0
32	26959.0
33	34952.0
34	61292.0
35	104212.0
36	123316.0
37	89139.0
38	146223.0
39	439430.0
40	1169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.96940765330456	19.084877353763304	25.817763759915064	28.12795123301707
2	34.02718303919109	19.15827276702433	26.058131582192946	20.756412611591646
3	20.658178327567782	21.268024352442875	39.15089010265871	18.922907217330632
4	22.07373522281243	23.911725413183404	36.562214040344195	17.452325323659974
5	19.39605320693108	28.654656037699887	32.79576152269178	19.15352923267726
6	70.95482171641771	1.6989615109623077	22.910925912030724	4.435290860589251
7	71.67445899990771	1.4949895340383088	19.23580799207916	7.594743473974814
8	59.20077483477407	3.3767927325603955	27.63928094918985	9.783151483475683
9	32.34029597929404	17.90114991897181	31.152342486629703	18.60621161510445
10	25.044308923105586	19.597610293642028	43.09319837546886	12.264882407783537
11	22.270721267334384	15.922061143295274	41.74379222284607	20.063425366524275
12	18.501422629073723	19.15508166209994	43.594633078993645	18.74886262983269
13	12.946916400813127	22.272187450678025	43.66630357067392	21.11459257783493
14	11.552231056695584	22.625451390416163	44.4404483761157	21.38186917677255
15	12.810906334170783	18.569298293276344	47.97110411367923	20.648691258873644
16	13.265854401094291	25.064921735995576	40.22379132588685	21.445432537023283
17	14.935405999104765	22.30176985578793	41.42847655788449	21.334347587222815
18	16.256264699566096	29.066567311183615	39.46517081467184	15.211997174578451
19	15.853581756539391	23.785116169156158	36.97490152853926	23.386400545765188
20	13.277066391369182	29.94153378302039	35.46887249638414	21.312527329226295
21	13.800666337206643	24.17330977090454	37.560339913046704	24.46568397884211
22	12.355958267247276	26.860910085875222	33.37637012677311	27.406761520104396
23	12.050215917058871	23.485238552342313	43.249821255001194	21.21472427559762
24	17.04645127570888	21.993440123228396	38.84316409265244	22.116944508410285
25	13.23963559306685	25.190668519232446	38.49740356179057	23.072292325910134
26	14.772659647960754	21.9700674358092	37.021905641614765	26.235367274615278
27	19.735259035786086	22.925846483704234	37.35697165867597	19.981922821833713
28	14.640875639191254	23.25961880957987	34.482648582588816	27.616856968640064
29	15.715070553604956	22.1586876106645	33.93507222677889	28.191169608951654
30	18.32858548668231	25.266565068785564	32.24758144432859	24.15726800020354
31	15.92283735800661	20.415740599393	31.120517683464815	32.540904359135574
32	13.649390714574638	22.411992344798023	34.46436441383284	29.474252526794505
33	12.641950265336064	21.703998281978105	36.08924054290182	29.56481090978401
34	12.867483762019468	20.127419957170197	35.55166873226026	31.453427548550074
35	14.125210332625254	19.391482164742087	38.00502469656473	28.478282806067927
36	17.915553014171092	20.0311693329642	36.915736718319444	25.13754093454526
37	14.154102769102861	19.590106884765753	39.09000037085814	27.165789975273245
38	16.302233859693153	19.450215744566712	33.16299732723401	31.08455306850612
39	23.064271440559633	17.05404093066419	35.28706576177281	24.594621867003372
40	16.97072721831384	17.26525757822735	38.84195664754591	26.922058555912905
41	18.663479011585434	21.558673638799696	31.003912984605936	28.77393436500893
42	19.10359275291447	17.731676373662864	37.50324500872379	25.661485864698875
43	21.806976100349036	20.00193191217044	35.92037072014613	22.270721267334384
44	14.68908719737329	22.317725380409893	37.41975880421536	25.573428618001454
45	16.796337646499744	20.063684104761386	36.232581526262365	26.907396722476506
46	21.833194908376477	17.794636011360335	34.1748363265035	26.19733275375968
47	16.18838903536348	16.64678694544849	38.95933756111612	28.2054864580719
48	17.710028607824416	14.760240212579337	34.575449363633304	32.95428181596294
49	19.00277108651948	14.995174531877845	39.928743489499105	26.073310892103567
50	17.49027359843653	14.478733010600505	40.625266823807024	27.40572656715594
51	15.479877496069335	14.24034884814049	38.552428560216576	31.727345095573593
52	14.16540100545679	14.545659967933707	46.44394479215988	24.844994234449615
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	562.0
1	2036.0
2	3510.0
3	46784.0
4	90058.0
5	49263.0
6	8468.0
7	7974.5
8	7481.0
9	7808.5
10	8136.0
11	8377.5
12	8619.0
13	8378.5
14	7644.5
15	7151.0
16	6432.5
17	5714.0
18	5235.5
19	4757.0
20	4351.0
21	3945.0
22	3893.0
23	3841.0
24	3799.5
25	3758.0
26	4748.0
27	5738.0
28	5699.5
29	5661.0
30	6002.5
31	6344.0
32	8185.5
33	10027.0
34	11713.0
35	13399.0
36	15145.5
37	16892.0
38	19213.0
39	23558.0
40	25582.0
41	30378.0
42	35174.0
43	42832.0
44	50490.0
45	60243.5
46	69997.0
47	89045.5
48	108094.0
49	138346.5
50	168599.0
51	172906.0
52	177213.0
53	150229.0
54	123245.0
55	95635.0
56	68025.0
57	52325.5
58	36626.0
59	28425.0
60	20224.0
61	15934.0
62	11644.0
63	9602.5
64	6017.5
65	4474.0
66	3643.5
67	2813.0
68	2234.0
69	1655.0
70	1535.0
71	1415.0
72	1445.5
73	1476.0
74	1083.5
75	691.0
76	507.0
77	323.0
78	280.0
79	237.0
80	159.0
81	81.0
82	60.5
83	40.0
84	36.0
85	32.0
86	22.0
87	12.0
88	10.0
89	7.0
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1159473.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.069462966602106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.4051550781809	22.80658747259226
2	11.972046061422391	7.439300834796351
3	4.668558822781738	4.351488463554622
4	2.396973093005671	2.9789066698032567
5	1.3953344747368877	2.167614639443047
6	0.8864210100663912	1.6524374847045462
7	0.628531491149384	1.366969512132629
8	0.47499594820835467	1.180629521771642
9	0.3630994102664984	1.0153173312423034
>10	3.2426755669061404	21.705297689793802
>50	0.35158480209444354	7.358037375447897
>100	0.19764359434394746	11.591961191704065
>500	0.010578107865828365	2.292117045527428
>1k	0.005289053932914183	3.057539875989949
>5k	8.351137788811868E-4	1.968044803070584
>10k+	2.783712596270622E-4	7.067750088425623
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81719	7.047943332876229	No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTT	8101	0.6986794862838548	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	8074	0.6963508421498388	No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	6580	0.5674992000676169	No Hit
CGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	3978	0.3430869024116991	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCG	3144	0.2711576724943142	No Hit
TCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	3135	0.27038145778297556	No Hit
ACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	2362	0.2037132386868862	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT	2273	0.19603733765253695	No Hit
GCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	2135	0.1841353787453438	No Hit
AGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	1840	0.15869278542924242	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC	1684	0.14523839709937186	No Hit
TCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	1619	0.1396324019619258	No Hit
CGTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	1612	0.13902867940866237	No Hit
ACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	1548	0.13350893035025396	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCT	1544	0.13316394603410342	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTT	1535	0.13238773132276474	No Hit
TGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTTG	1275	0.10996375077298048	Illumina Single End Adapter 1 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	1243	0.10720387624377627	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCC	1230	0.10608267721628704	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7189473148576982	0.0
2	0.0	0.0	0.0	2.4254122346962803	0.0
3	0.0	0.0	0.0	4.05874047951095	0.0
4	0.0	0.0	0.0	5.102059297629181	0.0
5	0.0	0.0	0.0	10.3666924542443	0.0
6	0.0	0.0	0.0	11.897215372846112	0.0
7	0.0	0.0	0.0	16.318879352947416	0.0
8	0.0	0.0	0.0	21.76023072551064	0.0
9	0.0	0.0	0.0	25.878222261320445	0.0
10	0.0	0.0	0.0	28.336494252130063	0.0
11	0.0	0.0	0.0	30.518865036098298	0.0
12	0.0	0.0	0.0	32.02213419372422	0.0
13	0.0	0.0	0.0	32.6672548649257	0.0
14	0.0	0.0	0.0	33.12203043969114	0.0
15	0.0	0.0	0.0	34.53767358101482	0.0
16	0.0	0.0	0.0	36.17755652783635	0.0
17	0.0	0.0	0.0	37.96923257376412	0.0
18	0.0	0.0	0.0	38.939673455095544	0.0
19	0.0	0.0	0.0	39.98937448306256	0.0
20	0.0	0.0	0.0	41.137913517606705	0.0
21	0.0	0.0	0.0	42.28145027956666	0.0
22	8.624607903763175E-5	0.0	0.0	43.357025131244974	0.0
23	1.724921580752635E-4	0.0	0.0	44.1817101390028	0.0
24	2.5873823711289524E-4	0.0	0.0	44.86728022127294	0.0
25	2.5873823711289524E-4	0.0	0.0	45.53827471618572	0.0
26	2.5873823711289524E-4	0.0	0.0	46.2221198768751	0.0
27	2.5873823711289524E-4	0.0	0.0	46.877762569719174	0.0
28	3.44984316150527E-4	0.0	0.0	47.52141705757702	0.0
29	3.44984316150527E-4	0.0	0.0	48.27201668344153	0.0
30	3.44984316150527E-4	0.0	0.0	48.940337549904136	0.0
31	3.44984316150527E-4	0.0	0.0	49.54069650608509	0.0
32	3.44984316150527E-4	0.0	0.0	50.142262907372576	0.0
33	3.44984316150527E-4	0.0	0.0	50.7152818564986	0.0
34	3.44984316150527E-4	0.0	0.0	51.36816467481347	0.0
35	3.44984316150527E-4	0.0	0.0	51.901941657977375	0.0
36	3.44984316150527E-4	0.0	0.0	52.410879770378436	0.0
37	3.44984316150527E-4	0.0	0.0	52.93534217700628	0.0
38	3.44984316150527E-4	0.0	0.0	53.446436441383284	0.0
39	3.44984316150527E-4	0.0	0.0	53.99815260898701	0.0
40	4.312303951881588E-4	0.0	0.0	54.535638173549536	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	35	1.01957994E-7	46.000004	37
CTCGCGG	70	0.0	46.000004	1
ACCGTAG	35	1.01957994E-7	46.000004	38
GCGTAGG	145	0.0	46.000004	1
GCGAGAC	25	3.417174E-5	46.0	20
CGGGTAC	110	0.0	46.0	5
AACCGGC	20	6.3114776E-4	46.0	2
ACACGTT	25	3.417174E-5	46.0	22
TGCCCGC	20	6.3114776E-4	46.0	28
ACGTTTC	25	3.417174E-5	46.0	24
TGGACCG	20	6.3114776E-4	46.0	22
GCAACCG	40	5.6115823E-9	46.0	35
CAACCGG	25	3.417174E-5	46.0	1
TCGGGCA	40	5.6115823E-9	46.0	4
GACCATA	60	0.0	46.0	8
CCCGTGA	30	1.8614173E-6	46.0	13
ACTAGGT	40	5.6115823E-9	46.0	34
TTGCGGA	30	1.8614173E-6	46.0	2
AGCGATG	20	6.3114776E-4	46.0	16
CGCAGCG	20	6.3114776E-4	46.0	25
>>END_MODULE