FastQCFastQC Report
Thu 26 May 2016
SRR1043331_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043331_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1130332
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT752586.658043831369899No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT78630.6956363263182852No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC77840.6886472293096187No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC65000.5750522855231914No Hit
CGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG40560.35883262616647144No Hit
TCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC30200.26717813881231356No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCC28560.2526691273006515No Hit
ACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG24270.21471567645612086No Hit
GCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG22960.20312616116326884No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC22720.20100289118595246No Hit
AGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG17980.15906830913395356No Hit
TCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG16340.1445592976222915No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTT16130.14270143639213967No Hit
ACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC15590.13792407894317776No Hit
TGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTT15400.13624315687780228Illumina Single End Adapter 1 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT14880.13164273859361675No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTC14120.12491905033211481No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCT14030.12412282409062117No Hit
TGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG13060.11554127459896739No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGC11510.10182848932879897No Hit
GGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG11410.10094379350491713No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCGG406.8157533E-945.0000041
GTACCGG302.1654705E-645.0000041
ATCTACG207.033235E-445.00000440
CGACGCG207.033235E-445.00000437
CGGTTAG302.1654705E-645.00000430
CTCCCGT302.1654705E-645.00000435
CCGTGGA302.1654705E-645.00000438
ATACTCG302.1654705E-645.00000443
CCGTACG207.033235E-445.00000413
TACGCGA207.033235E-445.0000041
TGATTCG302.1654705E-645.00000414
ACTACGG750.045.0000041
TCGTCGA207.033235E-445.00000425
GCTAGTA207.033235E-445.0000048
GCGATCG207.033235E-445.0000048
TTCGGCA302.1654705E-645.00000417
TTACGCT207.033235E-445.0000042
CGTCGAC207.033235E-445.00000426
CCGGTTA302.1654705E-645.00000429
ACGTAGG750.045.0000041