##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043331_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1130332
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10027761754954	33.0	31.0	34.0	30.0	34.0
2	32.39872090677783	34.0	31.0	34.0	30.0	34.0
3	32.46253401655443	34.0	31.0	34.0	31.0	34.0
4	36.05928612124579	37.0	35.0	37.0	35.0	37.0
5	36.00393866580792	37.0	35.0	37.0	35.0	37.0
6	36.263848143731224	37.0	37.0	37.0	35.0	37.0
7	36.19641220455583	37.0	37.0	37.0	35.0	37.0
8	36.09149258801839	37.0	37.0	37.0	35.0	37.0
9	37.61672234352385	39.0	37.0	39.0	35.0	39.0
10	37.45727980805639	39.0	37.0	39.0	35.0	39.0
11	37.230796792446824	39.0	37.0	39.0	34.0	39.0
12	37.255768216771706	39.0	37.0	39.0	34.0	39.0
13	37.2608242534052	39.0	37.0	39.0	34.0	39.0
14	38.650195694716245	40.0	38.0	41.0	34.0	41.0
15	38.763587158463174	40.0	38.0	41.0	35.0	41.0
16	38.80660991637855	40.0	38.0	41.0	35.0	41.0
17	38.762300810735255	40.0	38.0	41.0	35.0	41.0
18	38.512239766723404	40.0	38.0	41.0	35.0	41.0
19	38.24077793073186	40.0	37.0	41.0	34.0	41.0
20	37.9569427389475	40.0	35.0	41.0	34.0	41.0
21	37.88152949752816	40.0	35.0	41.0	34.0	41.0
22	37.86039057551233	40.0	35.0	41.0	34.0	41.0
23	37.81671933555805	40.0	35.0	41.0	34.0	41.0
24	37.73463548762665	40.0	35.0	41.0	34.0	41.0
25	37.71446176875467	39.0	35.0	41.0	34.0	41.0
26	37.704085171436354	40.0	35.0	41.0	34.0	41.0
27	37.704993754047486	40.0	35.0	41.0	34.0	41.0
28	37.654567861477865	40.0	35.0	41.0	34.0	41.0
29	37.64219273629341	40.0	35.0	41.0	34.0	41.0
30	37.52305694256201	40.0	35.0	41.0	33.0	41.0
31	37.40530658249081	40.0	35.0	41.0	33.0	41.0
32	37.21102207139141	40.0	35.0	41.0	33.0	41.0
33	36.98430903486763	40.0	35.0	41.0	33.0	41.0
34	36.70132138168255	40.0	35.0	41.0	32.0	41.0
35	36.569141632723834	40.0	35.0	41.0	31.0	41.0
36	36.50250103509411	40.0	35.0	41.0	31.0	41.0
37	36.32504697734825	39.0	35.0	41.0	30.0	41.0
38	36.262314965868434	39.0	35.0	41.0	30.0	41.0
39	36.18678671399199	39.0	35.0	41.0	30.0	41.0
40	36.10620861835284	39.0	35.0	41.0	30.0	41.0
41	36.05161580845274	39.0	35.0	41.0	30.0	41.0
42	35.98931552853498	39.0	35.0	41.0	30.0	41.0
43	35.87915231984939	39.0	35.0	41.0	30.0	41.0
44	35.79442853957952	38.0	35.0	40.0	29.0	41.0
45	35.75208345866524	38.0	35.0	40.0	29.0	41.0
46	35.671730960461176	38.0	35.0	40.0	28.0	41.0
47	35.525705721858714	38.0	35.0	40.0	27.0	41.0
48	35.5161961264478	38.0	35.0	40.0	27.0	41.0
49	35.45434792609605	38.0	35.0	40.0	27.0	41.0
50	35.3482277773256	38.0	35.0	40.0	27.0	41.0
51	34.925882837962654	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	5.0
15	8.0
16	35.0
17	78.0
18	182.0
19	367.0
20	625.0
21	1152.0
22	1915.0
23	2913.0
24	5310.0
25	9637.0
26	14946.0
27	17535.0
28	17431.0
29	16735.0
30	18279.0
31	21069.0
32	26063.0
33	34391.0
34	60635.0
35	101842.0
36	115456.0
37	89364.0
38	154398.0
39	419839.0
40	118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.251373932614488	19.28672283895351	25.953702098144614	27.508201130287386
2	33.62587275243026	19.275929549902152	26.11170877228991	20.986488925377678
3	20.888199219344404	21.363369346351337	38.56893372920523	19.17949770509903
4	22.371126359335133	24.084251352699916	35.97412087775981	17.570501410205143
5	19.689878725896463	28.913894324853228	32.15948942434612	19.23673752490419
6	71.51544855847662	1.655531295230074	22.355378773670036	4.4736413726232644
7	72.14110544512586	1.4739917121695219	18.632490277192897	7.752412565511725
8	59.4782771787404	3.3236252711592704	27.260132421270917	9.937965128829406
9	32.605199180417785	17.9242028005931	30.651790801286698	18.81880721770241
10	25.303981485085796	19.572479590067342	42.76221499524034	12.361323929606522
11	22.485340590198277	15.873920228746952	41.46976286613137	20.170976314923404
12	18.78616194180117	18.950626895460804	43.317007746396634	18.946203416341394
13	13.026349780418498	22.20869620607043	43.394949448480624	21.370004565030452
14	11.723546710170108	22.485694468527832	44.163219301939606	21.62753951936245
15	12.893026119759504	18.449358241649357	47.82939879610592	20.82821684248522
16	13.351829374024623	24.946298963490374	40.00116779848752	21.700703863997482
17	15.221987876128429	22.140397688466752	41.20408871021965	21.433525725185167
18	16.45773100292657	29.093929925013185	39.24307194700318	15.205267125057063
19	15.98645353754472	23.700912652211915	36.63551947569387	23.677114334549497
20	13.352714069848503	29.991896186253243	35.16648206013808	21.488907683760168
21	13.873888379697291	24.094602293839333	37.18571180856598	24.8457975178974
22	12.507387210129414	26.86847758003843	32.90502259513134	27.719112614700812
23	12.188896713531953	23.50105986559701	42.89686569963515	21.413177721235886
24	17.070117452217577	21.84968664073918	38.556459518088495	22.52373638895475
25	13.19718454401008	25.115275865851807	38.36103021059299	23.326509379545122
26	14.944104917847145	21.743611611455748	36.73531316462773	26.57697030606937
27	19.887077425039724	22.89283148667825	37.02089297657679	20.199198111705233
28	14.792379584051412	23.280593666285657	34.0673359685473	27.85969078111564
29	15.927267386927028	22.07378009292845	33.4301780361876	28.568774483956926
30	18.499166616533902	25.236921541635553	31.743328508792107	24.520583333038434
31	16.107656865416534	20.223881125191536	30.60437110512664	33.0640909042653
32	13.736406648666055	22.34201986672942	33.9954101980657	29.92616328653882
33	12.687511279871755	21.577554205313128	35.58432389775747	30.150610617057644
34	13.081554799828723	19.949802358952944	35.17249799174048	31.79614484947785
35	14.222016186394793	19.169235233542004	37.858876860957665	28.749871719105535
36	18.057437991669705	19.75870806099447	36.70010227083724	25.483751676498585
37	14.333930208115845	19.270002087882144	38.88043512879402	27.515632575207995
38	16.567079406758367	19.082535042801585	32.78222681477654	31.568158735663506
39	23.31987416086601	16.759854626782218	34.960878750667945	24.959392461683823
40	17.188312814288192	16.863275568594005	38.646698492124436	27.301713124993366
41	18.980883492637563	21.265875866559558	30.64789813966162	29.10534250114126
42	19.289023048095604	17.386750087584886	37.26533443271534	26.058892431604164
43	22.0142400639812	19.65475630168835	35.6957071019842	22.63529653234625
44	14.87580640024347	22.05272433232006	37.357254328816666	25.714214938619804
45	17.008808031622568	19.658737432895823	36.182289805119204	27.15016473036241
46	22.048831670694984	17.534494290173154	33.954979598914306	26.461694440217563
47	16.44525679180984	16.18781030706023	38.854513541154276	28.512419359975656
48	17.98082333332154	14.35905556951409	34.30823864139032	33.35188245577406
49	19.17949770509903	14.616767463010868	39.763892378522414	26.439842453367685
50	17.76938103141378	14.06692900846831	40.37371320992416	27.789976750193752
51	15.727591539476899	14.004115604972696	38.260351825835244	32.00794102971516
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	424.0
1	1865.0
2	3306.0
3	43283.5
4	83261.0
5	46019.5
6	8778.0
7	8031.5
8	7285.0
9	7519.5
10	7754.0
11	7921.5
12	8089.0
13	7775.0
14	7461.0
15	6813.0
16	6165.0
17	5585.0
18	5005.0
19	4593.0
20	4181.0
21	3809.0
22	3437.0
23	3488.5
24	3540.0
25	3582.5
26	4414.5
27	5204.0
28	5291.0
29	5378.0
30	6260.5
31	7143.0
32	8643.0
33	10143.0
34	12011.5
35	13880.0
36	15912.5
37	17945.0
38	20443.0
39	22941.0
40	26490.0
41	30039.0
42	35976.0
43	41913.0
44	47741.0
45	53569.0
46	63174.0
47	72779.0
48	98792.5
49	124806.0
50	156059.0
51	187312.0
52	180263.5
53	173215.0
54	134930.5
55	96646.0
56	72940.5
57	49235.0
58	37910.5
59	26586.0
60	20486.5
61	14387.0
62	11642.5
63	8898.0
64	7347.0
65	5796.0
66	4496.0
67	3196.0
68	2630.5
69	2065.0
70	1804.5
71	1544.0
72	1458.5
73	1373.0
74	1227.5
75	756.5
76	431.0
77	347.0
78	263.0
79	199.0
80	135.0
81	95.0
82	55.0
83	45.5
84	36.0
85	21.0
86	6.0
87	7.0
88	8.0
89	8.0
90	8.0
91	5.0
92	2.0
93	1.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1130332.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.512084244201898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.53101117794122	22.13072323378492
2	12.209057082210677	7.45047556469364
3	4.883376948111582	4.4700602651092245
4	2.4632624768755544	3.0063708884003337
5	1.494813932382813	2.2804944317135556
6	0.9045190089668446	1.6559256121247024
7	0.6717640249421919	1.4347844364882214
8	0.4646442032030211	1.1341810457368515
9	0.37370158087554106	1.0262172706079342
>10	3.4271456703114738	22.27888472208683
>50	0.35403980946986086	7.292392658261153
>100	0.20540103477788124	11.788878900694929
>500	0.010533386398751962	2.2303067247414163
>1k	0.00555928726600798	3.1243220774577942
>5k	8.777821998959968E-4	1.9772077108657495
>10k+	2.9259406663199893E-4	6.718774457232788
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	75258	6.658043831369899	No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	7863	0.6956363263182852	No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	7784	0.6886472293096187	No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	6500	0.5750522855231914	No Hit
CGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	4056	0.35883262616647144	No Hit
TCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	3020	0.26717813881231356	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCC	2856	0.2526691273006515	No Hit
ACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	2427	0.21471567645612086	No Hit
GCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	2296	0.20312616116326884	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC	2272	0.20100289118595246	No Hit
AGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	1798	0.15906830913395356	No Hit
TCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	1634	0.1445592976222915	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTT	1613	0.14270143639213967	No Hit
ACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	1559	0.13792407894317776	No Hit
TGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTT	1540	0.13624315687780228	Illumina Single End Adapter 1 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT	1488	0.13164273859361675	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTC	1412	0.12491905033211481	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCT	1403	0.12412282409062117	No Hit
TGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	1306	0.11554127459896739	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGC	1151	0.10182848932879897	No Hit
GGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	1141	0.10094379350491713	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.715276573608462	0.0
2	0.0	0.0	0.0	2.430701776115336	0.0
3	8.846958238818329E-5	0.0	0.0	4.120559269312025	0.0
4	8.846958238818329E-5	0.0	0.0	5.166092793975575	0.0
5	8.846958238818329E-5	0.0	0.0	10.473029163113138	0.0
6	8.846958238818329E-5	0.0	0.0	12.010365096272599	0.0
7	8.846958238818329E-5	0.0	0.0	16.564425319286723	0.0
8	8.846958238818329E-5	0.0	0.0	22.144644228421384	0.0
9	8.846958238818329E-5	0.0	0.0	26.392865105119558	0.0
10	8.846958238818329E-5	0.0	0.0	28.969099344263455	0.0
11	8.846958238818329E-5	0.0	0.0	31.193136175919996	0.0
12	8.846958238818329E-5	0.0	0.0	32.73586875360513	0.0
13	8.846958238818329E-5	0.0	0.0	33.38912814995948	0.0
14	1.7693916477636658E-4	0.0	0.0	33.859343980352676	0.0
15	1.7693916477636658E-4	0.0	0.0	35.32723129133741	0.0
16	1.7693916477636658E-4	0.0	0.0	37.03124391771621	0.0
17	1.7693916477636658E-4	0.0	0.0	38.892290052834035	0.0
18	1.7693916477636658E-4	0.0	0.0	39.89818920458768	0.0
19	1.7693916477636658E-4	0.0	0.0	40.99087701666413	0.0
20	1.7693916477636658E-4	0.0	0.0	42.17955432563176	0.0
21	1.7693916477636658E-4	0.0	0.0	43.359915493854906	0.0
22	1.7693916477636658E-4	0.0	0.0	44.449241461800604	0.0
23	1.7693916477636658E-4	0.0	0.0	45.27890920543699	0.0
24	5.308174943290997E-4	0.0	0.0	45.99055852616753	0.0
25	5.308174943290997E-4	0.0	0.0	46.668058588096244	0.0
26	5.308174943290997E-4	0.0	0.0	47.37324962931245	0.0
27	5.308174943290997E-4	0.0	0.0	48.03137485269814	0.0
28	5.308174943290997E-4	0.0	0.0	48.681183935339355	0.0
29	5.308174943290997E-4	0.0	0.0	49.445915005502805	0.0
30	5.308174943290997E-4	0.0	0.0	50.12713078989182	0.0
31	5.308174943290997E-4	0.0	0.0	50.73394365549237	0.0
32	5.308174943290997E-4	0.0	0.0	51.32801690122902	0.0
33	5.308174943290997E-4	0.0	0.0	51.9143932932979	0.0
34	5.308174943290997E-4	0.0	0.0	52.555532356865065	0.0
35	5.308174943290997E-4	0.0	0.0	53.104663054748514	0.0
36	5.308174943290997E-4	0.0	0.0	53.60814344811967	0.0
37	5.308174943290997E-4	0.0	0.0	54.13135255836338	0.0
38	5.308174943290997E-4	0.0	0.0	54.669336088865926	0.0
39	5.308174943290997E-4	0.0	0.0	55.216608925519225	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGG	40	6.8157533E-9	45.000004	1
GTACCGG	30	2.1654705E-6	45.000004	1
ATCTACG	20	7.033235E-4	45.000004	40
CGACGCG	20	7.033235E-4	45.000004	37
CGGTTAG	30	2.1654705E-6	45.000004	30
CTCCCGT	30	2.1654705E-6	45.000004	35
CCGTGGA	30	2.1654705E-6	45.000004	38
ATACTCG	30	2.1654705E-6	45.000004	43
CCGTACG	20	7.033235E-4	45.000004	13
TACGCGA	20	7.033235E-4	45.000004	1
TGATTCG	30	2.1654705E-6	45.000004	14
ACTACGG	75	0.0	45.000004	1
TCGTCGA	20	7.033235E-4	45.000004	25
GCTAGTA	20	7.033235E-4	45.000004	8
GCGATCG	20	7.033235E-4	45.000004	8
TTCGGCA	30	2.1654705E-6	45.000004	17
TTACGCT	20	7.033235E-4	45.000004	2
CGTCGAC	20	7.033235E-4	45.000004	26
CCGGTTA	30	2.1654705E-6	45.000004	29
ACGTAGG	75	0.0	45.000004	1
>>END_MODULE