FastQCFastQC Report
Thu 26 May 2016
SRR1043330_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043330_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2845548
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1201064.220839008865779No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC172470.6061046940694728No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA121530.4270882093712705No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT92930.3265803282882594Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCTT78890.2772400957565994TruSeq Adapter, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT56800.19961005753549055Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC52350.18397159352082623TruSeq Adapter, Index 27 (95% over 22bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC42900.15076182162451662No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC38590.13561535423053836No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT35680.12538885304342082No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC34280.12046888683656012No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG32300.1135106489154286No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC29570.10391671481205025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGG1650.046.0000041
TACCGAT206.313032E-446.00000440
CGTTTTT293700.045.2795371
TCACGAC4100.043.1951224
ACGGGTA2150.042.7906954
TAGTAGG5200.042.4615361
TATTAGG3650.041.5890431
CATGCGG3550.041.464791
TAACGGG7050.041.4326252
TCGGCGT19750.040.875954
GCATAGG4450.040.831461
CGCACTT47800.040.65899734
CCGCACT47900.040.6221333
CACTTAC47450.040.5711336
ACCCGCA48650.040.5159331
CCCGCAC49200.040.2967532
CTTACTG47350.040.26821538
GTTTTTT340300.040.194242
ACTTACT47450.040.18335337
GCGTAGG5050.040.079211
Produced by FastQC (version 0.11.5)