##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043330_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2845548
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36918758706583	33.0	31.0	34.0	31.0	34.0
2	32.69908256687288	34.0	31.0	34.0	31.0	34.0
3	32.824252481420096	34.0	31.0	34.0	31.0	34.0
4	36.248247789178045	37.0	37.0	37.0	35.0	37.0
5	36.18875239496926	37.0	37.0	37.0	35.0	37.0
6	36.332952738804615	37.0	37.0	37.0	35.0	37.0
7	36.27410818583977	37.0	37.0	37.0	35.0	37.0
8	36.25459770842031	37.0	37.0	37.0	35.0	37.0
9	37.86060505744412	39.0	38.0	39.0	35.0	39.0
10	37.64649867090627	39.0	37.0	39.0	35.0	39.0
11	37.286083383587275	39.0	37.0	39.0	35.0	39.0
12	37.288564100834	39.0	37.0	39.0	35.0	39.0
13	37.27689604954828	39.0	37.0	39.0	35.0	39.0
14	38.55952139974444	40.0	37.0	41.0	35.0	41.0
15	38.617038967538065	40.0	38.0	41.0	35.0	41.0
16	38.49334996281911	40.0	37.0	41.0	34.0	41.0
17	38.44095899981304	40.0	37.0	41.0	34.0	41.0
18	38.26584088548146	40.0	37.0	41.0	34.0	41.0
19	38.061409612489406	40.0	37.0	41.0	34.0	41.0
20	37.764827372442845	40.0	35.0	41.0	34.0	41.0
21	37.70523533604072	40.0	35.0	41.0	34.0	41.0
22	37.66547919768003	39.0	35.0	41.0	34.0	41.0
23	37.684759490966236	39.0	35.0	41.0	34.0	41.0
24	37.60607236286297	39.0	35.0	41.0	34.0	41.0
25	37.50631301949572	39.0	35.0	41.0	34.0	41.0
26	37.4111503302703	39.0	35.0	41.0	34.0	41.0
27	37.43253742337153	39.0	35.0	41.0	34.0	41.0
28	37.393907605846046	39.0	35.0	41.0	33.0	41.0
29	37.29840684465699	39.0	35.0	41.0	33.0	41.0
30	37.207090163300705	39.0	35.0	41.0	33.0	41.0
31	37.062411528464814	39.0	35.0	41.0	33.0	41.0
32	36.836023851996174	39.0	35.0	41.0	32.0	41.0
33	36.35415217033766	39.0	35.0	41.0	31.0	41.0
34	36.17665349521428	39.0	35.0	41.0	30.0	41.0
35	35.94723406528374	38.0	35.0	41.0	30.0	41.0
36	35.88946874204898	38.0	35.0	41.0	30.0	41.0
37	35.814653627350516	38.0	35.0	41.0	30.0	41.0
38	35.764091486068764	38.0	35.0	41.0	30.0	41.0
39	35.77026780078916	38.0	35.0	41.0	30.0	41.0
40	35.71462895723425	38.0	35.0	41.0	30.0	41.0
41	35.685003732145795	38.0	35.0	41.0	30.0	41.0
42	35.59483586289882	37.0	35.0	41.0	29.0	41.0
43	35.4489472678022	37.0	35.0	40.0	29.0	41.0
44	35.25952224316722	37.0	35.0	40.0	28.0	41.0
45	35.12011183786041	37.0	35.0	40.0	28.0	41.0
46	35.080104429797004	36.0	35.0	40.0	28.0	41.0
47	34.978028133772476	36.0	35.0	40.0	27.0	41.0
48	34.86522279715542	36.0	35.0	40.0	27.0	41.0
49	34.79163345689477	35.0	35.0	40.0	27.0	41.0
50	34.65839971773451	35.0	35.0	40.0	27.0	41.0
51	34.46240442965643	35.0	34.0	39.0	27.0	41.0
52	34.04092884744872	35.0	34.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	3.0
12	5.0
13	6.0
14	33.0
15	79.0
16	145.0
17	281.0
18	556.0
19	1091.0
20	1965.0
21	3265.0
22	5093.0
23	8093.0
24	13770.0
25	23912.0
26	36176.0
27	45230.0
28	48692.0
29	48804.0
30	51474.0
31	58142.0
32	69457.0
33	90232.0
34	210955.0
35	288383.0
36	266346.0
37	282507.0
38	429043.0
39	860097.0
40	1711.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.384603598322713	17.461416922153482	23.918942853889654	29.235036625634148
2	34.78458279389418	19.83410576802781	24.154328094272177	21.226983343805834
3	26.09188107176544	20.84491282522734	33.24607421839308	19.81713188461414
4	23.26152994080578	23.442865838144357	31.287646527136424	22.00795769391344
5	21.83867571378167	31.586464188971686	28.185572691095	18.389287406151645
6	78.78749541388864	2.13435162576769	16.235396486019564	2.842756474324102
7	78.76468785625826	1.9198410991485646	14.810714842975765	4.504756201617404
8	69.5506454292811	3.4133319838568883	20.098553951646572	6.937468635215431
9	43.699210134568105	14.458585833027593	24.258666520473383	17.58353751193092
10	29.4344006848593	15.125592680214847	34.18772060777046	21.2522860271554
11	25.654390648128235	12.536706462164757	39.04003727928681	22.768865610420207
12	21.88031971346117	13.823383053106115	41.30480315215206	22.991494081280653
13	17.8921951061799	16.642277691326942	43.396421357151596	22.069105845341568
14	18.043308353962047	16.954800973309887	43.002964631065794	21.998926041662273
15	21.704395778950136	14.509402055421312	41.639220283755535	22.146981881873018
16	21.343727113371482	18.359521610600137	37.43964255742655	22.85710871860183
17	21.402626137390758	19.550188575276188	37.77075628314827	21.276429004184784
18	22.52100474144172	24.349650752684546	33.90436569687104	19.224978809002693
19	23.40670408652393	21.211415165022697	32.527653724344134	22.85422702410924
20	22.652332696549134	24.801022509548247	33.682404935710096	18.864239858192516
21	20.765631084065355	19.574507265384383	34.74824532919494	24.911616321355325
22	22.40004385798447	20.81950471403048	32.411612807093746	24.3688386208913
23	22.492433794826162	18.957543503044054	35.46012929671192	23.089893405417865
24	24.999859429536947	17.772393929042842	32.53492824580713	24.692818395613077
25	19.630489452295304	18.60773390573626	35.30146038654066	26.46031625542778
26	19.505522310641044	18.254620902546716	35.11580194746319	27.12405483934905
27	23.175746815727585	20.135875409587186	34.38560867713355	22.302769097551682
28	24.124000016868454	21.619807502807895	30.7115536269288	23.544638853394847
29	25.442621245538643	18.92566915054675	33.20685505920125	22.424854544713355
30	29.075559435300335	18.428682278422293	30.09413301058355	22.40162527569382
31	29.722042994881832	19.6836250873294	29.193181770260068	21.4011501475287
32	31.483074613396084	18.030551584439973	28.26116445760184	22.225209344562103
33	25.70977541057118	20.790758054336106	33.17568355901921	20.323782976073502
34	26.066262104874	22.169508298577288	31.759822712532	20.004406884016717
35	25.85301671242235	26.38068308810816	29.62824735340961	18.138052846059878
36	25.919506541446495	22.379731426073292	30.0852770714112	21.615484961069008
37	23.380909406553677	26.196465496276993	31.74467624513802	18.677948852031314
38	21.790143761412565	22.733160712804704	29.777533185172068	25.699162340610666
39	23.634885090674977	20.244501234911517	32.621765649358224	23.49884802505528
40	20.148772749572313	19.8071162391216	34.29750613941497	25.74660487189111
41	21.777878988511176	23.457660879380704	28.88831957851352	25.876140553594595
42	21.63235341663539	18.359381040137084	31.241855698796854	28.76640984443067
43	23.655021809507343	19.282015274386517	32.649844599353095	24.413118316753046
44	23.818329544959354	19.723195672678866	33.09780752248776	23.36066725987402
45	23.659414636477756	21.126510605338584	32.14712948085922	23.06694527732444
46	23.916518013401987	20.138616533616723	31.665253933512982	24.279611519468304
47	21.57180268967524	20.81567416891228	33.7091133236902	23.90340981772228
48	23.026847552738523	18.924685157305376	31.03331941685749	27.01514787309861
49	22.091280835888202	17.65958613244268	35.0883204219363	25.16081260973282
50	21.411025222558187	17.7717613619591	36.25674211083419	24.560471304648523
51	20.113630133809025	16.567810488524533	35.29527528616632	28.023284091500127
52	17.97857565572607	17.691144201398114	38.49342903370458	25.83685110917124
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	822.0
1	4763.5
2	8705.0
3	85528.0
4	162351.0
5	92204.0
6	22057.0
7	20069.0
8	18081.0
9	17570.0
10	17059.0
11	16534.0
12	16009.0
13	15156.5
14	13254.5
15	12205.0
16	11413.5
17	10622.0
18	9899.5
19	9177.0
20	8939.5
21	8702.0
22	8302.5
23	7903.0
24	8285.5
25	8668.0
26	9234.0
27	9800.0
28	10528.5
29	11257.0
30	11993.5
31	12730.0
32	15839.0
33	18948.0
34	21694.0
35	24440.0
36	26911.5
37	29383.0
38	32618.0
39	39016.0
40	42179.0
41	49656.5
42	57134.0
43	73025.0
44	88916.0
45	113740.0
46	138564.0
47	178086.5
48	217609.0
49	246500.5
50	275392.0
51	265633.5
52	255875.0
53	228431.5
54	200988.0
55	190180.0
56	179372.0
57	168780.5
58	158189.0
59	156786.5
60	155384.0
61	152541.5
62	149699.0
63	147320.5
64	124270.5
65	103599.0
66	86920.0
67	70241.0
68	58658.0
69	47075.0
70	40393.5
71	33712.0
72	31849.0
73	29986.0
74	23934.5
75	17883.0
76	13979.5
77	10076.0
78	7602.0
79	5128.0
80	3897.5
81	2667.0
82	1882.5
83	1098.0
84	771.5
85	445.0
86	322.5
87	200.0
88	132.5
89	43.0
90	21.0
91	21.5
92	22.0
93	15.0
94	8.0
95	5.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2845548.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.087109380166847
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.94394917629778	20.029278393398762
2	10.588535953951371	5.736256631210202
3	3.861204654995254	3.137666184871975
4	1.9329379671557956	2.0943080856570555
5	1.158025801639096	1.5683785777026795
6	0.7487332648788629	1.2168611905405917
7	0.6074743678599609	1.1518307253509328
8	0.47460249067229143	1.0284487661551978
9	0.3960917687394409	0.9656082957986086
>10	5.522018028510574	34.71741183398292
>50	0.5918688259464276	10.41073591266486
>100	0.1625582261213532	7.925052039427529
>500	0.0059997366158773425	1.1382445840685784
>1k	0.00508673321780905	2.605300464428865
>5k	5.217162274675949E-4	0.9926518740634068
>10k+	3.9128717060069615E-4	5.281966440677785
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	120106	4.220839008865779	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	17247	0.6061046940694728	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	12153	0.4270882093712705	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	9293	0.3265803282882594	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCTT	7889	0.2772400957565994	TruSeq Adapter, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	5680	0.19961005753549055	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	5235	0.18397159352082623	TruSeq Adapter, Index 27 (95% over 22bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4290	0.15076182162451662	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3859	0.13561535423053836	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT	3568	0.12538885304342082	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3428	0.12046888683656012	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG	3230	0.1135106489154286	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	2957	0.10391671481205025	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.28946972604222454	0.0
2	0.0	0.0	0.0	0.9713067570815885	0.0
3	0.0	0.0	0.0	1.6207071537714353	0.0
4	0.0	0.0	0.0	2.048322502379155	0.0
5	0.0	0.0	0.0	4.315688928810901	0.0
6	0.0	0.0	0.0	5.120700828100598	0.0
7	0.0	0.0	0.0	7.471074112965235	0.0
8	3.514261576329059E-5	0.0	0.0	10.167039881246072	0.0
9	3.514261576329059E-5	0.0	0.0	12.687362855941984	0.0
10	3.514261576329059E-5	0.0	0.0	14.117702460123674	0.0
11	7.028523152658117E-5	0.0	0.0	15.269536834381286	0.0
12	7.028523152658117E-5	0.0	0.0	16.06470880125726	0.0
13	7.028523152658117E-5	0.0	0.0	16.46582661757946	0.0
14	7.028523152658117E-5	0.0	0.0	16.737127611272065	0.0
15	7.028523152658117E-5	0.0	0.0	17.44303733410928	0.0
16	7.028523152658117E-5	0.0	0.0	18.185846803497956	0.0
17	7.028523152658117E-5	0.0	0.0	19.147419056013113	0.0
18	7.028523152658117E-5	0.0	0.0	19.71029833269374	0.0
19	7.028523152658117E-5	0.0	0.0	20.327156667186777	0.0
20	7.028523152658117E-5	0.0	0.0	21.019641910802417	0.0
21	7.028523152658117E-5	0.0	0.0	21.707382901290014	0.0
22	1.0542784728987176E-4	0.0	0.0	22.358505286152262	0.0
23	1.0542784728987176E-4	0.0	0.0	22.950517791300655	0.0
24	1.7571307881645293E-4	0.0	0.0	23.43787558670597	0.0
25	1.7571307881645293E-4	0.0	0.0	23.895643299638593	0.0
26	1.7571307881645293E-4	0.0	0.0	24.33327429373885	0.0
27	1.7571307881645293E-4	0.0	0.0	24.795751117183755	0.0
28	2.1085569457974352E-4	0.0	0.0	25.251796841943978	0.0
29	2.459983103430341E-4	0.0	0.0	25.734726667763116	0.0
30	2.459983103430341E-4	0.0	0.0	26.184657577380527	0.0
31	2.459983103430341E-4	0.0	0.0	26.659118032800713	0.0
32	2.459983103430341E-4	0.0	0.0	27.1703376643093	0.0
33	2.459983103430341E-4	0.0	0.0	27.65467319475897	0.0
34	2.459983103430341E-4	0.0	0.0	28.1793524481049	0.0
35	2.459983103430341E-4	0.0	0.0	28.651493490884707	0.0
36	2.459983103430341E-4	0.0	0.0	29.147355799304737	0.0
37	2.811409261063247E-4	0.0	0.0	29.764916986113043	0.0
38	2.811409261063247E-4	0.0	0.0	30.422294756581156	0.0
39	2.811409261063247E-4	0.0	0.0	31.161941390551135	0.0
40	2.811409261063247E-4	0.0	0.0	31.80895208936908	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACGG	165	0.0	46.000004	1
TACCGAT	20	6.313032E-4	46.000004	40
CGTTTTT	29370	0.0	45.279537	1
TCACGAC	410	0.0	43.19512	24
ACGGGTA	215	0.0	42.790695	4
TAGTAGG	520	0.0	42.461536	1
TATTAGG	365	0.0	41.589043	1
CATGCGG	355	0.0	41.46479	1
TAACGGG	705	0.0	41.432625	2
TCGGCGT	1975	0.0	40.87595	4
GCATAGG	445	0.0	40.83146	1
CGCACTT	4780	0.0	40.658997	34
CCGCACT	4790	0.0	40.62213	33
CACTTAC	4745	0.0	40.57113	36
ACCCGCA	4865	0.0	40.51593	31
CCCGCAC	4920	0.0	40.29675	32
CTTACTG	4735	0.0	40.268215	38
GTTTTTT	34030	0.0	40.19424	2
ACTTACT	4745	0.0	40.183353	37
GCGTAGG	505	0.0	40.07921	1
>>END_MODULE