##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043329_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2796340
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3555261520416	33.0	31.0	34.0	31.0	34.0
2	32.642010985788566	34.0	31.0	34.0	31.0	34.0
3	32.74244762797085	34.0	31.0	34.0	31.0	34.0
4	36.220572605620205	37.0	37.0	37.0	35.0	37.0
5	36.206987347747415	37.0	37.0	37.0	35.0	37.0
6	36.35396053412675	37.0	37.0	37.0	35.0	37.0
7	36.30652209674074	37.0	37.0	37.0	35.0	37.0
8	36.21295300285373	37.0	37.0	37.0	35.0	37.0
9	37.82089230923278	39.0	38.0	39.0	35.0	39.0
10	37.59316177574973	39.0	37.0	39.0	35.0	39.0
11	37.276079089095035	39.0	37.0	39.0	35.0	39.0
12	37.28555361651301	39.0	37.0	39.0	35.0	39.0
13	37.28570381284107	39.0	37.0	39.0	35.0	39.0
14	38.56778288763169	40.0	37.0	41.0	35.0	41.0
15	38.62891958774684	40.0	38.0	41.0	35.0	41.0
16	38.541343327349324	40.0	38.0	41.0	34.0	41.0
17	38.46774748421151	40.0	37.0	41.0	34.0	41.0
18	38.31291223527897	40.0	37.0	41.0	34.0	41.0
19	38.133048556327196	40.0	37.0	41.0	34.0	41.0
20	37.85399557993663	40.0	35.0	41.0	34.0	41.0
21	37.79516761194991	40.0	35.0	41.0	34.0	41.0
22	37.770310834877016	40.0	35.0	41.0	34.0	41.0
23	37.7558254718668	40.0	35.0	41.0	34.0	41.0
24	37.68174864286889	40.0	35.0	41.0	34.0	41.0
25	37.64605162462362	39.0	35.0	41.0	34.0	41.0
26	37.61620225008404	39.0	35.0	41.0	34.0	41.0
27	37.58956993784733	39.0	35.0	41.0	34.0	41.0
28	37.495247716658206	39.0	35.0	41.0	34.0	41.0
29	37.430641838975234	39.0	35.0	41.0	34.0	41.0
30	37.29435226045474	39.0	35.0	41.0	33.0	41.0
31	37.13846885571855	39.0	35.0	41.0	33.0	41.0
32	36.93112640093837	39.0	35.0	41.0	33.0	41.0
33	36.449927405108106	39.0	35.0	41.0	31.0	41.0
34	36.22021749858744	39.0	35.0	41.0	31.0	41.0
35	35.99511683128661	38.0	35.0	41.0	30.0	41.0
36	35.91877275295565	38.0	35.0	41.0	30.0	41.0
37	35.79422173269345	38.0	35.0	41.0	30.0	41.0
38	35.73668581073832	38.0	35.0	41.0	30.0	41.0
39	35.69949791513192	38.0	35.0	40.0	30.0	41.0
40	35.62128639578878	38.0	35.0	40.0	29.0	41.0
41	35.55556691961635	38.0	35.0	40.0	29.0	41.0
42	35.45289700107998	37.0	35.0	40.0	29.0	41.0
43	35.29408083423332	37.0	35.0	40.0	28.0	41.0
44	35.15511454258066	37.0	35.0	40.0	28.0	41.0
45	35.053613294520694	36.0	35.0	40.0	27.0	41.0
46	34.954542366092824	36.0	35.0	40.0	27.0	41.0
47	34.79666814478926	36.0	35.0	40.0	26.0	41.0
48	34.71220917341954	36.0	35.0	40.0	26.0	41.0
49	34.6223463527325	35.0	34.0	40.0	27.0	41.0
50	34.519925688578645	35.0	34.0	39.0	27.0	41.0
51	34.07819936059278	35.0	34.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	10.0
14	23.0
15	62.0
16	125.0
17	263.0
18	546.0
19	972.0
20	1801.0
21	2964.0
22	4690.0
23	7230.0
24	11755.0
25	20663.0
26	33522.0
27	43835.0
28	47978.0
29	48725.0
30	51058.0
31	57268.0
32	66817.0
33	87401.0
34	204811.0
35	287522.0
36	254480.0
37	278555.0
38	441727.0
39	841348.0
40	187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.664883383279573	17.49111338392327	23.99833353597917	28.845669696817982
2	34.566719354584926	19.901192272756532	24.17924858922735	21.3528397834312
3	26.08842272398921	20.94909774920074	33.03432343706416	19.928156089745883
4	23.489024939742663	23.53912614345895	30.918235979888	22.05361293691039
5	21.996574093279072	31.623014368782048	27.910196900233874	18.470214637705002
6	79.08480370770364	2.1264581560182236	15.943340223291875	2.8453979129862605
7	79.06388350486708	1.9041675904932875	14.519979687734683	4.511969216904954
8	69.84690702847294	3.3371478432522514	19.827202700673023	6.988742427601793
9	43.86515945843495	14.438444538217812	24.04922148236624	17.647174520980997
10	29.755323029388414	15.08818670118798	33.85807877439797	21.29841149502564
11	25.876610140397805	12.555662043957458	38.853751689708695	22.713976125936046
12	22.085905147442727	13.809086162626864	41.05891987383509	23.046088816095324
13	17.9827202700673	16.601700794610096	43.2566140025891	22.1589649327335
14	18.152835492107542	16.84408905927033	42.87711794703076	22.125957501591365
15	21.846377765221682	14.491728473647697	41.42153672300221	22.240357038128412
16	21.49788652309805	18.232081935673058	37.36444781392821	22.905583727300684
17	21.50743471823884	19.476386991567548	37.70947023609432	21.30670805409929
18	22.650428774755575	24.447492078931745	33.72193653132309	19.18014261498959
19	23.498358568700517	21.297875079568293	32.23813985423804	22.96562649749315
20	22.686154044214938	24.925259446276204	33.42819542687942	18.96039108262944
21	20.858407775878472	19.715664046575164	34.49022651036712	24.93570166717924
22	22.45199081656737	20.924279594040783	32.147914774312135	24.475814815079712
23	22.588526430977634	19.025583441212444	35.11146713203688	23.274422995773048
24	25.072523369833426	17.78961070542209	32.386512369740444	24.75135355500404
25	19.54483360392513	18.68867877296752	35.18352560847393	26.582962014633416
26	19.533032463863478	18.14357338521067	35.00261055522576	27.320783595700092
27	23.13831651372866	20.078209373681315	34.28406416959311	22.499409942996916
28	24.245406495633578	21.69957873506083	30.449516153257473	23.60549861604812
29	25.539955799366314	18.974659733794887	32.91663388572205	22.568750581116745
30	29.216046689601406	18.362716980052497	29.900405530085756	22.52083080026034
31	29.956443064863358	19.60376778217241	28.951128975732566	21.488660177231665
32	31.719676434196124	17.95901070685253	28.013689322471517	22.307623536479827
33	25.962472374603944	20.630717294749566	32.93190384574122	20.47490648490527
34	26.32773554002732	22.160037763648198	31.502714262214184	20.009512434110302
35	26.028773325132136	26.423753906892582	29.428109600406245	18.119363167569034
36	26.04733329995637	22.370598711172462	29.892895713682886	21.68917227518828
37	23.479154895327465	26.058240414255778	31.828497249976756	18.634107440440005
38	21.88653740246179	22.71798136135091	29.740410679674145	25.655070556513156
39	23.60814493230437	20.232232132001116	32.51507327435147	23.644549661343042
40	20.156740596637032	19.746704621040358	34.12914738551106	25.96740739681155
41	21.837473268629708	23.368867877296754	28.82871896836579	25.964939885707743
42	21.712917599433545	18.274852128138924	31.171424075756164	28.840806196671366
43	23.800646559431257	19.14641996323766	32.56338642654327	24.489547050787817
44	23.852750380854975	19.62790647775306	33.10101775892774	23.41832538246422
45	23.723974910061006	21.027557450095482	32.217541500675885	23.030926139167626
46	24.085554689343926	20.111145282762468	31.62337197908695	24.179928048806655
47	21.753041475643162	20.636403298597454	33.655385253581464	23.955169972177917
48	23.101947545720478	18.806582890492574	30.997089052118127	27.094380511668824
49	22.203701981876307	17.574186257751204	34.98380025318809	25.23831150718439
50	21.556284285887983	17.676391282891206	36.22574508107025	24.541579350150556
51	20.197257844182037	16.564938455266525	35.09394422709685	28.143859473454587
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	807.0
1	4536.0
2	8265.0
3	81768.0
4	155271.0
5	89018.5
6	22766.0
7	20218.0
8	17670.0
9	17169.0
10	16668.0
11	16084.0
12	15500.0
13	14552.5
14	13605.0
15	12517.5
16	11430.0
17	10647.0
18	9864.0
19	9059.0
20	8254.0
21	8160.5
22	8067.0
23	7803.5
24	7540.0
25	7952.0
26	9134.5
27	9905.0
28	10410.5
29	10916.0
30	12747.0
31	14578.0
32	17107.0
33	19636.0
34	22262.0
35	24888.0
36	28392.0
37	31896.0
38	34876.0
39	37856.0
40	42920.0
41	47984.0
42	57354.0
43	66724.0
44	82200.5
45	97677.0
46	128527.0
47	159377.0
48	209036.0
49	258695.0
50	277776.5
51	296858.0
52	261310.0
53	225762.0
54	205615.0
55	185468.0
56	174912.5
57	164357.0
58	161644.5
59	158932.0
60	157680.5
61	156429.0
62	149620.5
63	142812.0
64	135860.0
65	128908.0
66	106117.0
67	83326.0
68	69365.0
69	55404.0
70	46943.0
71	38482.0
72	34635.5
73	30789.0
74	25886.0
75	16702.5
76	12422.0
77	9140.0
78	5858.0
79	4344.0
80	2830.0
81	2130.5
82	1431.0
83	1042.0
84	653.0
85	449.0
86	245.0
87	183.5
88	122.0
89	78.0
90	34.0
91	27.0
92	20.0
93	13.0
94	6.0
95	4.5
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2796340.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.637053970262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.2073771575213	19.5002885636622
2	10.840865859256631	5.775374579547791
3	3.9357590144776777	3.145110758477612
4	2.0005285514615503	2.1315274797732546
5	1.1976486888247897	1.5950916380819717
6	0.788046146547442	1.2594736641984725
7	0.5952957248222133	1.109984704524887
8	0.48194537230819634	1.027008391431315
9	0.4226160442089583	1.0131521740463387
>10	5.769088103745453	35.451457692512676
>50	0.5784497432416909	9.989999961655691
>100	0.16891804655910794	7.971581424472106
>500	0.0072066867911415745	1.2874325562850464
>1k	0.005303033676500403	2.6155037207611698
>5k	5.439008898974773E-4	1.00404889274941
>10k+	4.0792566742310795E-4	5.122963797820038
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	114736	4.103077594283957	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	16525	0.5909510288448472	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	10180	0.3640472903867198	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	8989	0.32145590307330296	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	7710	0.2757175450767789	TruSeq Adapter, Index 27 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	5869	0.20988148794495662	No Hit
CGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG	5153	0.18427659011422073	TruSeq Adapter, Index 27 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4102	0.14669174706938357	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3733	0.1334959268186272	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	3442	0.12308946694607951	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3319	0.1186908601958274	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCC	3134	0.1120750695552043	No Hit
GGGCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	2931	0.10481558036576381	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.286517376284715	0.0
2	0.0	0.0	0.0	0.9735225330253117	0.0
3	0.0	0.0	0.0	1.6290937439653261	0.0
4	0.0	0.0	0.0	2.0658432093379204	0.0
5	0.0	0.0	0.0	4.34593075234056	0.0
6	0.0	0.0	0.0	5.163249104186186	0.0
7	0.0	0.0	0.0	7.533669010206198	0.0
8	0.0	0.0	0.0	10.30990509022508	0.0
9	0.0	0.0	0.0	12.940200404814865	0.0
10	0.0	0.0	0.0	14.429861890900249	0.0
11	0.0	0.0	0.0	15.605470007223728	0.0
12	0.0	0.0	0.0	16.427186965819608	0.0
13	0.0	0.0	0.0	16.849596257965768	0.0
14	0.0	0.0	0.0	17.13021306421966	0.0
15	0.0	0.0	0.0	17.854230887517254	0.0
16	0.0	0.0	0.0	18.620875859158758	0.0
17	0.0	0.0	0.0	19.595363940007296	0.0
18	0.0	0.0	0.0	20.153522103892946	0.0
19	0.0	0.0	0.0	20.794860424697998	0.0
20	0.0	0.0	0.0	21.504037420342303	0.0
21	0.0	0.0	0.0	22.216468669761188	0.0
22	3.57610304898546E-5	0.0	0.0	22.887238318659392	0.0
23	3.57610304898546E-5	0.0	0.0	23.48931102798658	0.0
24	1.0728309146956378E-4	0.0	0.0	23.98442249511862	0.0
25	1.0728309146956378E-4	0.0	0.0	24.45156883640759	0.0
26	1.430441219594184E-4	0.0	0.0	24.898295629286853	0.0
27	1.430441219594184E-4	0.0	0.0	25.369804816295588	0.0
28	1.7880515244927297E-4	0.0	0.0	25.839597473840808	0.0
29	1.7880515244927297E-4	0.0	0.0	26.32566140025891	0.0
30	1.7880515244927297E-4	0.0	0.0	26.782329759614353	0.0
31	1.7880515244927297E-4	0.0	0.0	27.26041897623322	0.0
32	1.7880515244927297E-4	0.0	0.0	27.786749822982898	0.0
33	1.7880515244927297E-4	0.0	0.0	28.29040817640201	0.0
34	1.7880515244927297E-4	0.0	0.0	28.827932225695015	0.0
35	1.7880515244927297E-4	0.0	0.0	29.307308839411515	0.0
36	1.7880515244927297E-4	0.0	0.0	29.813899597330796	0.0
37	1.7880515244927297E-4	0.0	0.0	30.448157234098858	0.0
38	1.7880515244927297E-4	0.0	0.0	31.115815673344443	0.0
39	1.7880515244927297E-4	0.0	0.0	31.85925173619803	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATA	20	7.035024E-4	45.0	30
ACGATAA	20	7.035024E-4	45.0	21
CGTTTTT	29085	0.0	44.21867	1
ACTACGG	150	0.0	43.5	1
GCATAGG	540	0.0	43.333336	1
ATAACGG	130	0.0	43.26923	1
TATGCGG	575	0.0	41.869564	1
CGCACTT	4340	0.0	41.370968	34
GCACTTA	4365	0.0	41.13402	35
CACTTAC	4370	0.0	40.932495	36
CCGCACT	4395	0.0	40.904438	33
CTTACTG	4405	0.0	40.45403	38
ACCCGCA	4500	0.0	40.4	31
CCCGCAC	4545	0.0	40.297028	32
ATACCGG	95	0.0	40.26316	1
ACTTACT	4425	0.0	40.118645	37
CGACGGT	345	0.0	39.782608	27
ACGACCA	380	0.0	39.671055	27
GTTTTTT	33325	0.0	39.59865	2
AAACACG	365	0.0	39.452057	39
>>END_MODULE