FastQCFastQC Report
Thu 26 May 2016
SRR1043328_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043328_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2625822
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1018473.8786711361242308No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC141170.5376221236626092No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT114430.43578734582922984Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCTT94920.3614868029896924Illumina Single End Adapter 1 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA85810.32679290523119997No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT61600.23459320548003632Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC46730.17796331967665746Illumina Single End Adapter 1 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC35340.13458642665039747No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC33880.12902626301401998No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCG32150.12243784993803844No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC29880.11379293798284881No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA27410.1043863597761006No Hit
TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT26540.10107311158182085Illumina Single End Adapter 1 (95% over 22bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC26480.10084461170635328No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT26400.10053994520572987No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA253.4183635E-546.01
AATCGGT253.4183635E-546.046
CGTTTTT261600.045.22631
ACGTAGG2000.044.8500021
CTATCGG850.043.2941171
AATGCGG3800.042.9736821
TCACGAC3300.042.51515224
ACGGGTA2800.041.892864
CGGTCTA3200.041.68750430
CTTACGG2100.041.6190451
CACTTAC38100.041.2309736
TAGGGTA7650.041.189544
CCGCACT38500.041.1610433
ACCCGCA39350.041.03176531
ACGGGAT3650.040.9589044
TAGTAGG7300.040.9589041
CGGGTAC4900.040.8367355
ACTTACT37850.040.7741137
CGCACTT38700.040.77002734
TATACGG1750.040.742861
Produced by FastQC (version 0.11.5)