##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043328_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2625822
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.346142274685796	33.0	31.0	34.0	31.0	34.0
2	32.68331135926198	34.0	31.0	34.0	31.0	34.0
3	32.80425443918133	34.0	31.0	34.0	31.0	34.0
4	36.234697172923376	37.0	37.0	37.0	35.0	37.0
5	36.16873992220341	37.0	37.0	37.0	35.0	37.0
6	36.33277807863595	37.0	37.0	37.0	35.0	37.0
7	36.26985949542657	37.0	37.0	37.0	35.0	37.0
8	36.238899666466345	37.0	37.0	37.0	35.0	37.0
9	37.800544743703114	39.0	38.0	39.0	35.0	39.0
10	37.62275356059931	39.0	37.0	39.0	35.0	39.0
11	37.240606560536094	39.0	37.0	39.0	35.0	39.0
12	37.223185349197315	39.0	37.0	39.0	34.0	39.0
13	37.15934248399168	39.0	37.0	39.0	34.0	39.0
14	38.49696475998754	40.0	37.0	41.0	34.0	41.0
15	38.552443006418564	40.0	37.0	41.0	35.0	41.0
16	38.41511572376193	40.0	37.0	41.0	34.0	41.0
17	38.3579945632263	40.0	37.0	41.0	34.0	41.0
18	38.20286257027323	40.0	37.0	41.0	34.0	41.0
19	38.004233341026165	40.0	36.0	41.0	34.0	41.0
20	37.77044102760964	40.0	35.0	41.0	34.0	41.0
21	37.68680816902288	39.0	35.0	41.0	34.0	41.0
22	37.6280288610576	39.0	35.0	41.0	34.0	41.0
23	37.64897734880734	39.0	35.0	41.0	34.0	41.0
24	37.55330521261533	39.0	35.0	41.0	34.0	41.0
25	37.45060137358892	39.0	35.0	41.0	34.0	41.0
26	37.35770931921509	39.0	35.0	41.0	33.0	41.0
27	37.39033567393373	39.0	35.0	41.0	34.0	41.0
28	37.372505447817865	39.0	35.0	41.0	33.0	41.0
29	37.27221304414389	39.0	35.0	41.0	33.0	41.0
30	37.22974405728949	39.0	35.0	41.0	33.0	41.0
31	37.13746209758315	39.0	35.0	41.0	33.0	41.0
32	36.943258530090766	39.0	35.0	41.0	33.0	41.0
33	36.59252873957184	39.0	35.0	41.0	31.0	41.0
34	36.34780841961108	39.0	35.0	41.0	31.0	41.0
35	36.24855607120361	39.0	35.0	41.0	31.0	41.0
36	36.14298532040633	39.0	35.0	41.0	31.0	41.0
37	36.11321026330041	39.0	35.0	41.0	31.0	41.0
38	35.95961569367611	38.0	35.0	41.0	30.0	41.0
39	35.90856767899728	38.0	35.0	41.0	30.0	41.0
40	35.83515904733832	38.0	35.0	41.0	30.0	41.0
41	35.80579643250761	38.0	35.0	41.0	30.0	41.0
42	35.74046146311517	38.0	35.0	41.0	30.0	41.0
43	35.640546465068844	38.0	35.0	41.0	30.0	41.0
44	35.486339896611426	37.0	35.0	40.0	29.0	41.0
45	35.39466308074196	37.0	35.0	40.0	29.0	41.0
46	35.34867481497223	37.0	35.0	40.0	28.0	41.0
47	35.26653139474039	37.0	35.0	40.0	28.0	41.0
48	35.166237086900786	37.0	35.0	40.0	28.0	41.0
49	35.12516956594925	36.0	35.0	40.0	28.0	41.0
50	35.02755441914951	36.0	35.0	40.0	28.0	41.0
51	34.90189967179801	36.0	35.0	40.0	27.0	41.0
52	34.555704461307734	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	0.0
11	0.0
12	2.0
13	10.0
14	18.0
15	49.0
16	104.0
17	225.0
18	526.0
19	1034.0
20	1876.0
21	3062.0
22	4929.0
23	7744.0
24	12774.0
25	22100.0
26	33015.0
27	40079.0
28	42470.0
29	43667.0
30	46495.0
31	53053.0
32	63412.0
33	81998.0
34	199813.0
35	266647.0
36	231436.0
37	238347.0
38	362347.0
39	866580.0
40	2006.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.39586156258878	17.346187212994636	24.34788039707185	28.91007082734473
2	34.18304820357206	20.18263233379871	24.534336295453386	21.09998316717584
3	26.561663357226806	20.59126627775988	32.79769154192478	20.049378823088542
4	23.575588901304048	23.86852574165347	31.002710770189296	21.55317458685318
5	22.018857333056086	31.248462386254666	28.231273863955742	18.501406416733506
6	78.50463588164011	2.1529258266554248	16.38043248933096	2.962005802373504
7	78.45516565860137	2.020472065509391	14.818331174009511	4.706031101879716
8	69.09977142395792	3.320255523794073	20.497809828693644	7.082163223554376
9	42.03936900521056	15.034301639638938	25.394866826464245	17.531462528686255
10	30.34634487790871	15.572647346240528	34.19473978053348	19.886267995317276
11	27.139996542035217	13.057968133407366	38.265503145300784	21.53653217925663
12	22.431185358337313	14.127119050720117	41.03701621815949	22.404679372783075
13	17.135891160939316	17.38838352333098	43.034562129496976	22.44116318623273
14	18.390050810755643	17.25288309717871	42.56282413659418	21.79424195547147
15	22.6765561412769	14.336234520085519	41.431330836591364	21.555878502046216
16	21.669328690215863	18.187599921091376	37.65114314679365	22.49192824189911
17	21.819148441897433	18.84678397850273	38.02382644368125	21.31024113591858
18	22.354866399931144	25.196947850996754	33.878838702699575	18.569347046372524
19	22.92802025422896	21.786625292955883	32.47821063270854	22.807143820106617
20	22.83505888822624	25.29939196183138	33.161539510294304	18.70400963964808
21	19.463695558952587	20.366688983487837	35.64396215737396	24.525653300185617
22	20.91436510167102	21.08132234401266	33.73697836334679	24.26733419096953
23	22.707936790841117	19.398306511256287	35.810310066714344	22.083446631188252
24	24.486160905042308	17.746899827939593	32.7417471557478	25.0251921112703
25	18.337076922959746	18.943249009262622	36.26045482138546	26.45921924639218
26	17.800901965175093	17.73079058671913	36.95273327742703	27.515574170678743
27	21.406820416616206	20.517879734422213	35.02004324740977	23.055256601551818
28	22.67072939445248	21.62210538261923	31.352962995968504	24.354202226959785
29	22.815407898936026	18.238707726570954	36.264148902705514	22.6817354717875
30	25.171584364819854	18.074759065922976	32.17731438003033	24.576342189226843
31	28.072771117006408	18.220541986471282	31.378136065582513	22.328550830939797
32	25.00001904165629	17.510364373518083	30.62774247454702	26.86187411027861
33	22.027159495198077	18.023537010505663	36.63176711902025	23.31753637527601
34	21.189821701547174	21.70562208710263	32.6653139474039	24.4392422639463
35	24.13301434750718	22.96244756879941	30.774325144659464	22.130212939033946
36	22.252726955597144	23.142657803918162	31.38255372984155	23.222061510643144
37	24.129244099561966	24.633771824594355	30.680030862716514	20.556953213127166
38	22.92017509183791	22.371089891089344	29.781721685628348	24.9270133314444
39	24.88527402085899	18.65088341860187	34.454049055876595	22.00979350466254
40	19.369629776885105	18.8782407946921	36.63397595114977	25.11815347727302
41	20.963530658209127	22.381753218611163	31.222108733950737	25.432607389228973
42	20.744589694198616	16.79397918061468	33.29205102249886	29.169380102687843
43	22.89511627216163	17.90795415683165	33.622157175924336	25.57477239508238
44	21.04453386406238	19.65700645359815	33.616787428850856	25.681672253488625
45	22.890736691215167	20.198094158705352	32.716536002821215	24.19463314725827
46	22.843284883743074	19.459696811131906	32.88989124167594	24.807127063449084
47	20.223152978381627	19.511680532800778	35.17584207916607	25.08932440965153
48	21.716171164686713	18.283836451975798	32.63389521452711	27.366097168810377
49	22.054389063691293	16.71141455894573	35.54688779361282	25.687308583750156
50	20.3379360824915	17.233917607514904	36.7295269824078	25.6986193275858
51	18.886085957083154	16.164157357200907	36.36895417891997	28.580802506795965
52	17.76830264960839	16.754144035658168	39.40251852562741	26.075034789106038
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	837.0
1	4739.0
2	8641.0
3	74306.0
4	139971.0
5	80179.5
6	20388.0
7	18412.5
8	16437.0
9	16030.5
10	15624.0
11	15110.5
12	14597.0
13	13976.5
14	12546.0
15	11736.0
16	10890.5
17	10045.0
18	9975.5
19	9906.0
20	9141.0
21	8376.0
22	7855.5
23	7335.0
24	7471.5
25	7608.0
26	8644.5
27	9681.0
28	10291.0
29	10901.0
30	12426.0
31	13951.0
32	15866.5
33	17782.0
34	20462.5
35	23143.0
36	25701.5
37	28260.0
38	31007.0
39	36947.5
40	40141.0
41	48201.5
42	56262.0
43	74164.0
44	92066.0
45	115158.5
46	138251.0
47	177986.0
48	217721.0
49	246793.5
50	275866.0
51	263577.5
52	251289.0
53	222436.5
54	193584.0
55	178199.5
56	162815.0
57	152413.0
58	142011.0
59	137410.5
60	132810.0
61	128087.5
62	123365.0
63	121423.0
64	101042.5
65	82604.0
66	68930.5
67	55257.0
68	45495.5
69	35734.0
70	31094.0
71	26454.0
72	26190.0
73	25926.0
74	20482.0
75	15038.0
76	11438.0
77	7838.0
78	6177.0
79	4516.0
80	3547.5
81	2579.0
82	1797.0
83	1015.0
84	727.0
85	439.0
86	310.5
87	182.0
88	150.0
89	103.0
90	88.0
91	60.0
92	32.0
93	20.5
94	9.0
95	5.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2625822.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.85877558184581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.35673199887749	20.15769958592489
2	11.50830285031963	6.412144528699421
3	4.417558343926087	3.692032995694418
4	2.2441825631986365	2.500807135713692
5	1.2933234276022707	1.801520356215763
6	0.810726599932803	1.3551510243456506
7	0.634320955435493	1.2369983601037593
8	0.4903067753428252	1.0927477136427404
9	0.4167009500304377	1.0447900426475916
>10	5.1258551893396245	32.9416255377345
>50	0.5213905909383859	9.535413917852244
>100	0.167681830018682	8.418882687403574
>500	0.007420935659874932	1.4577721710867317
>1k	0.004672440971032365	2.5468995372940313
>5k	4.1227420332638515E-4	0.9277567303607104
>10k+	4.1227420332638515E-4	4.8777576752802805
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	101847	3.8786711361242308	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	14117	0.5376221236626092	No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	11443	0.43578734582922984	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCTT	9492	0.3614868029896924	Illumina Single End Adapter 1 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	8581	0.32679290523119997	No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	6160	0.23459320548003632	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	4673	0.17796331967665746	Illumina Single End Adapter 1 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3534	0.13458642665039747	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3388	0.12902626301401998	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCG	3215	0.12243784993803844	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2988	0.11379293798284881	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	2741	0.1043863597761006	No Hit
TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	2654	0.10107311158182085	Illumina Single End Adapter 1 (95% over 22bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2648	0.10084461170635328	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT	2640	0.10053994520572987	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3718073806983109	0.0
2	0.0	0.0	0.0	1.2206082514351697	0.0
3	0.0	0.0	0.0	1.9245021178130124	0.0
4	0.0	0.0	0.0	2.393840862023397	0.0
5	0.0	0.0	0.0	4.8468631917928935	0.0
6	0.0	0.0	0.0	5.578215126539423	0.0
7	0.0	0.0	0.0	7.930469011227722	0.0
8	0.0	0.0	0.0	10.611419966776118	0.0
9	0.0	0.0	0.0	13.192059476994253	0.0
10	0.0	0.0	0.0	14.650650348728893	0.0
11	3.808331257792798E-5	0.0	0.0	15.917148991820467	0.0
12	3.808331257792798E-5	0.0	0.0	16.862452976629793	0.0
13	3.808331257792798E-5	0.0	0.0	17.342226548486533	0.0
14	3.808331257792798E-5	0.0	0.0	17.650015880741346	0.0
15	3.808331257792798E-5	0.0	0.0	18.479927428439552	0.0
16	3.808331257792798E-5	0.0	0.0	19.39434584674818	0.0
17	3.808331257792798E-5	0.0	0.0	20.48413791947817	0.0
18	3.808331257792798E-5	0.0	0.0	21.155927553352818	0.0
19	3.808331257792798E-5	0.0	0.0	21.92597213367852	0.0
20	3.808331257792798E-5	0.0	0.0	22.740117189969464	0.0
21	3.808331257792798E-5	0.0	0.0	23.524633429074782	0.0
22	1.904165628896399E-4	0.0	0.0	24.28946059557731	0.0
23	1.904165628896399E-4	0.0	0.0	24.974350888978766	0.0
24	3.808331257792798E-4	0.0	0.0	25.553102990225536	0.0
25	3.808331257792798E-4	0.0	0.0	26.106377355357676	0.0
26	3.808331257792798E-4	0.0	0.0	26.65580530591944	0.0
27	3.808331257792798E-4	0.0	0.0	27.234328907290745	0.0
28	4.569997509351357E-4	0.0	0.0	27.831856081638435	0.0
29	4.569997509351357E-4	0.0	0.0	28.417082346023456	0.0
30	4.569997509351357E-4	0.0	0.0	28.962397298826804	0.0
31	4.569997509351357E-4	0.0	0.0	29.508055001443356	0.0
32	4.569997509351357E-4	0.0	0.0	30.113122671681477	0.0
33	4.569997509351357E-4	0.0	0.0	30.69941526881868	0.0
34	4.569997509351357E-4	0.0	0.0	31.30996693606802	0.0
35	4.569997509351357E-4	0.0	0.0	31.85215905723998	0.0
36	4.569997509351357E-4	0.0	0.0	32.43746148825016	0.0
37	4.569997509351357E-4	0.0	0.0	33.145811102199616	0.0
38	5.712496886689197E-4	0.0	0.0	33.947426748652425	0.0
39	5.712496886689197E-4	0.0	0.0	34.80133078327472	0.0
40	5.712496886689197E-4	0.0	0.0	35.61947458738635	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGA	25	3.4183635E-5	46.0	1
AATCGGT	25	3.4183635E-5	46.0	46
CGTTTTT	26160	0.0	45.2263	1
ACGTAGG	200	0.0	44.850002	1
CTATCGG	85	0.0	43.294117	1
AATGCGG	380	0.0	42.973682	1
TCACGAC	330	0.0	42.515152	24
ACGGGTA	280	0.0	41.89286	4
CGGTCTA	320	0.0	41.687504	30
CTTACGG	210	0.0	41.619045	1
CACTTAC	3810	0.0	41.23097	36
TAGGGTA	765	0.0	41.18954	4
CCGCACT	3850	0.0	41.16104	33
ACCCGCA	3935	0.0	41.031765	31
ACGGGAT	365	0.0	40.958904	4
TAGTAGG	730	0.0	40.958904	1
CGGGTAC	490	0.0	40.836735	5
ACTTACT	3785	0.0	40.77411	37
CGCACTT	3870	0.0	40.770027	34
TATACGG	175	0.0	40.74286	1
>>END_MODULE