##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043327_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2564185
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3403178787802	33.0	31.0	34.0	31.0	34.0
2	32.62782989526887	34.0	31.0	34.0	31.0	34.0
3	32.727163211702745	34.0	31.0	34.0	31.0	34.0
4	36.20696205616989	37.0	37.0	37.0	35.0	37.0
5	36.189396630898315	37.0	37.0	37.0	35.0	37.0
6	36.35435391752155	37.0	37.0	37.0	35.0	37.0
7	36.30268915854355	37.0	37.0	37.0	35.0	37.0
8	36.20091296064832	37.0	37.0	37.0	35.0	37.0
9	37.77617722590219	39.0	38.0	39.0	35.0	39.0
10	37.58272511538754	39.0	37.0	39.0	35.0	39.0
11	37.243211390753785	39.0	37.0	39.0	35.0	39.0
12	37.23324058131531	39.0	37.0	39.0	35.0	39.0
13	37.18179733521567	39.0	37.0	39.0	34.0	39.0
14	38.51903197312206	40.0	37.0	41.0	35.0	41.0
15	38.588104602437035	40.0	37.0	41.0	35.0	41.0
16	38.491732070813924	40.0	37.0	41.0	34.0	41.0
17	38.410979317014956	40.0	37.0	41.0	34.0	41.0
18	38.27440219796934	40.0	37.0	41.0	34.0	41.0
19	38.08975873425669	40.0	37.0	41.0	34.0	41.0
20	37.87448838519842	40.0	35.0	41.0	34.0	41.0
21	37.795568572470394	40.0	35.0	41.0	34.0	41.0
22	37.75496307793704	40.0	35.0	41.0	34.0	41.0
23	37.73575112560131	39.0	35.0	41.0	34.0	41.0
24	37.64110584844697	39.0	35.0	41.0	34.0	41.0
25	37.60904575917884	39.0	35.0	41.0	34.0	41.0
26	37.58836589403651	39.0	35.0	41.0	34.0	41.0
27	37.559554010338566	39.0	35.0	41.0	34.0	41.0
28	37.47201781462726	39.0	35.0	41.0	34.0	41.0
29	37.41710680001638	39.0	35.0	41.0	34.0	41.0
30	37.330050288883214	39.0	35.0	41.0	33.0	41.0
31	37.22610225081264	39.0	35.0	41.0	33.0	41.0
32	37.03946439121982	39.0	35.0	41.0	33.0	41.0
33	36.68267266207391	39.0	35.0	41.0	32.0	41.0
34	36.397707263711474	39.0	35.0	41.0	31.0	41.0
35	36.289696336262786	39.0	35.0	41.0	31.0	41.0
36	36.17599120188286	39.0	35.0	41.0	31.0	41.0
37	36.08996776753627	39.0	35.0	41.0	31.0	41.0
38	35.94657756753121	38.0	35.0	41.0	30.0	41.0
39	35.85500110171458	38.0	35.0	41.0	30.0	41.0
40	35.76065494494352	38.0	35.0	40.0	30.0	41.0
41	35.686580336442184	38.0	35.0	40.0	30.0	41.0
42	35.60388934495756	38.0	35.0	40.0	30.0	41.0
43	35.492288192934595	37.0	35.0	40.0	29.0	41.0
44	35.39378710974442	37.0	35.0	40.0	29.0	41.0
45	35.33759030647165	37.0	35.0	40.0	29.0	41.0
46	35.23024391765805	37.0	35.0	40.0	28.0	41.0
47	35.08683733817958	37.0	35.0	40.0	27.0	41.0
48	35.016102582301976	36.0	35.0	40.0	27.0	41.0
49	34.95911371449408	36.0	35.0	40.0	27.0	41.0
50	34.89318009425997	36.0	35.0	40.0	27.0	41.0
51	34.5071283858224	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	3.0
13	3.0
14	15.0
15	43.0
16	109.0
17	202.0
18	444.0
19	897.0
20	1634.0
21	2720.0
22	4425.0
23	6794.0
24	11060.0
25	19113.0
26	29979.0
27	38690.0
28	41730.0
29	43026.0
30	45333.0
31	51438.0
32	60658.0
33	78966.0
34	192240.0
35	263410.0
36	220770.0
37	234363.0
38	375752.0
39	840172.0
40	195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.563311539534006	17.465315490106992	24.342120400829113	28.62925256952989
2	33.9268422520216	20.208955282087683	24.6287221865817	21.235480279309023
3	26.671125523314426	20.642894330947257	32.509861807942876	20.17611833779544
4	23.699889048567087	24.02591856671808	30.650362590842704	21.623829793872126
5	22.18907762115448	31.30168065096707	27.928172109266686	18.58106961861176
6	78.83034960426022	2.1216097902452438	16.09993038723805	2.948110218256483
7	78.7696285564419	2.0031705980652723	14.509093532642925	4.718107312849892
8	69.42221407581746	3.2835774329855294	20.217963992457644	7.076244498739366
9	42.22004262562958	15.026216907126436	25.09362623991639	17.660114227327593
10	30.59693430856198	15.53105567656	33.9123347184388	19.95967529643922
11	27.320571643621655	12.99617617293604	38.09717317588239	21.586079007559906
12	22.568496422840006	14.105144519603693	40.866864130318206	22.45949492723809
13	17.211238658677125	17.34410738694751	42.87904343875344	22.565610515621923
14	18.446094958047098	17.223991248681354	42.42981688138726	21.900096911884283
15	22.7641141337306	14.311954870650908	41.26991617219507	21.654014823423427
16	21.81320770537227	18.127280207941315	37.39379178959397	22.66572029709245
17	21.95621610765214	18.75531601659007	37.87320337651144	21.41526449924635
18	22.53523049233967	25.23640064971911	33.65907686067893	18.569291997262287
19	23.06990330260882	21.772844003065302	32.25761791758395	22.899634776741927
20	22.91851016989804	25.362678589883338	32.888734627181734	18.83007661303689
21	19.513841629991596	20.373841980980313	35.47111460366549	24.6412017853626
22	21.10873435419051	21.13989435239657	33.41806460922282	24.333306684190102
23	22.8309189859546	19.4111969300187	35.51799109658624	22.239892987440456
24	24.50427718748842	17.77098766274664	32.52943917853041	25.195295971234525
25	18.331126654278066	18.98088476455482	36.11997574277987	26.568012838387244
26	17.855458946994855	17.677000684427995	36.81711732967785	27.650423038899298
27	21.41284657698255	20.5018748647231	34.92719909054924	23.158079467745114
28	22.74172885341736	21.574691373672337	31.153290421712942	24.53028935119736
29	22.93020979375513	18.3177110855886	35.999430618305624	22.75264850235065
30	25.1881592006817	18.06613017391491	32.01859460218354	24.727116023219853
31	28.248780801697226	18.297041750107734	31.127239259257816	22.326938188937227
32	25.096122159672568	17.43091859596714	30.419022028441784	27.05393721591851
33	22.167667309496	17.87909218718618	36.45477997882368	23.49846052449414
34	21.280133843696923	21.622230845278324	32.53938385880894	24.558251452215814
35	24.281828339218894	22.932120732318456	30.569869178705904	22.216181749756743
36	22.36028211692994	23.086906755947798	31.23830768840782	23.314503438714446
37	24.272936625087503	24.563477284205312	30.522992685785148	20.64059340492203
38	23.115180846935772	22.269376039560328	29.617714790469485	24.997728323034416
39	24.979827898533063	18.611566638132583	34.295146411042886	22.113459052291468
40	19.456006489391367	18.801685525810345	36.52002488120007	25.22228310359822
41	21.072504518979716	22.299288077888296	31.073459988261376	25.55474741487061
42	20.896113189960943	16.690527399544106	33.22318787450983	29.190171535985115
43	23.013394119379065	17.75671412164099	33.54496652932608	25.684925229653864
44	21.10850036171337	19.4993340964088	33.60393263356583	25.788232908311997
45	23.027433668007575	20.06079904531069	32.67389053441932	24.237876752262416
46	23.015227060450005	19.332692453937604	32.829807521688174	24.82227296392421
47	20.37368598599555	19.381206894198353	35.101406489781354	25.143700630024746
48	21.859616213338743	18.16776090648686	32.603653792530565	27.36896908764383
49	22.086432921181583	16.639361044542415	35.42345813582094	25.85074789845506
50	20.479723576887	17.091668502857633	36.62711543823866	25.8014924820167
51	18.9857986065748	16.16782720435538	36.18042379937485	28.66595038969497
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	725.0
1	4492.5
2	8260.0
3	70476.5
4	132693.0
5	76686.0
6	20679.0
7	18538.0
8	16397.0
9	15712.5
10	15028.0
11	14591.5
12	14155.0
13	13388.5
14	12622.0
15	11779.5
16	10937.0
17	10144.0
18	9351.0
19	9177.5
20	9004.0
21	8293.0
22	7582.0
23	7240.0
24	6898.0
25	6997.5
26	8482.5
27	9868.0
28	10168.5
29	10469.0
30	12630.5
31	14792.0
32	16638.0
33	18484.0
34	21573.0
35	24662.0
36	27606.0
37	30550.0
38	32429.5
39	34309.0
40	40571.5
41	46834.0
42	57381.5
43	67929.0
44	82447.0
45	96965.0
46	126919.0
47	156873.0
48	207805.5
49	258738.0
50	275763.0
51	292788.0
52	255694.0
53	218600.0
54	196110.0
55	173620.0
56	160295.5
57	146971.0
58	141535.5
59	136100.0
60	132821.0
61	129542.0
62	123921.5
63	118301.0
64	111025.5
65	103750.0
66	85126.0
67	66502.0
68	54258.0
69	42014.0
70	36097.5
71	30181.0
72	27731.5
73	25282.0
74	21787.0
75	14217.5
76	10143.0
77	7532.5
78	4922.0
79	3927.0
80	2932.0
81	2121.0
82	1310.0
83	917.5
84	525.0
85	378.0
86	231.0
87	187.5
88	144.0
89	129.5
90	115.0
91	61.0
92	7.0
93	6.0
94	5.0
95	5.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2564185.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.506863108327494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.57435179023203	19.687858967611874
2	11.830318446742934	6.508299000849589
3	4.514242778425248	3.725179744316978
4	2.2846263851442052	2.51371620919339
5	1.3164628379918575	1.810588153592117
6	0.8982646283506315	1.4825065300256095
7	0.6486040165794676	1.2488743326893956
8	0.5142827502587229	1.1317044168272696
9	0.40376658088007966	0.9995716861187214
>10	5.312075435570497	33.52425989206213
>50	0.51900984723825	9.317802803430256
>100	0.17082266719889305	8.411839133791744
>500	0.007444950436283809	1.4257917241151787
>1k	0.004867852208339413	2.5478682295700916
>5k	4.295163713240659E-4	0.9049626568457269
>10k+	4.295163713240659E-4	4.759176518959864
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	96392	3.7591671427763593	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	13545	0.5282380171477488	No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	10909	0.42543732219009156	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	9372	0.36549624929558516	Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	7432	0.28983868168638377	No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	6175	0.24081725772516416	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG	4609	0.1797452211911387	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3407	0.1328687282703861	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3218	0.1254979652404175	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCC	3140	0.12245606303757334	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2825	0.1101714579876257	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	2704	0.10545260969859818	No Hit
TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	2659	0.10369766612003423	Illumina Single End Adapter 1 (95% over 21bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2583	0.10073376140957069	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC	2570	0.10022677770909665	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3767668869445847	0.0
2	0.0	0.0	0.0	1.2237026579595467	0.0
3	0.0	0.0	0.0	1.939485645536496	0.0
4	0.0	0.0	0.0	2.408913553429257	0.0
5	0.0	0.0	0.0	4.86380662861689	0.0
6	0.0	0.0	0.0	5.5903922688885554	0.0
7	0.0	0.0	0.0	7.9602290786351215	0.0
8	0.0	0.0	0.0	10.654418460446497	0.0
9	0.0	0.0	0.0	13.28929074930241	0.0
10	0.0	0.0	0.0	14.794798347233137	0.0
11	0.0	0.0	0.0	16.093534592862838	0.0
12	0.0	0.0	0.0	17.057895588656823	0.0
13	0.0	0.0	0.0	17.540271080284768	0.0
14	0.0	0.0	0.0	17.857759873020083	0.0
15	0.0	0.0	0.0	18.694828961248895	0.0
16	0.0	0.0	0.0	19.65536807991623	0.0
17	0.0	0.0	0.0	20.75992956826438	0.0
18	0.0	0.0	0.0	21.448647425985254	0.0
19	0.0	0.0	0.0	22.23283421438001	0.0
20	3.899874619030998E-5	0.0	0.0	23.072555217349763	0.0
21	3.899874619030998E-5	0.0	0.0	23.868909614555893	0.0
22	1.1699623857092994E-4	0.0	0.0	24.639095853068323	0.0
23	1.1699623857092994E-4	0.0	0.0	25.3290226719211	0.0
24	1.949937309515499E-4	0.0	0.0	25.920750647866672	0.0
25	1.949937309515499E-4	0.0	0.0	26.48042165444381	0.0
26	1.949937309515499E-4	0.0	0.0	27.0507393187309	0.0
27	1.949937309515499E-4	0.0	0.0	27.646601161772647	0.0
28	2.3399247714185988E-4	0.0	0.0	28.233688286921577	0.0
29	2.3399247714185988E-4	0.0	0.0	28.827756187638567	0.0
30	2.3399247714185988E-4	0.0	0.0	29.373231650602435	0.0
31	2.3399247714185988E-4	0.0	0.0	29.92401094304818	0.0
32	2.3399247714185988E-4	0.0	0.0	30.536018267012714	0.0
33	2.3399247714185988E-4	0.0	0.0	31.124938333232585	0.0
34	2.3399247714185988E-4	0.0	0.0	31.742132490440433	0.0
35	2.3399247714185988E-4	0.0	0.0	32.280588179090046	0.0
36	2.3399247714185988E-4	0.0	0.0	32.87145818261943	0.0
37	2.3399247714185988E-4	0.0	0.0	33.575814537562614	0.0
38	3.509887157127898E-4	0.0	0.0	34.378057745443485	0.0
39	3.509887157127898E-4	0.0	0.0	35.24004703248791	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTACG	20	7.0349127E-4	45.0	39
ACGTAGG	310	0.0	45.0	1
CGCGTAT	20	7.0349127E-4	45.0	30
CGTTTTT	24905	0.0	44.241116	1
TCACGAC	320	0.0	42.890625	24
AATGCGG	390	0.0	42.115387	1
ACGGGTA	205	0.0	41.707314	4
ACTACGG	190	0.0	41.447372	1
CTTACGG	180	0.0	41.25	1
TATACGG	110	0.0	40.909092	1
CGCACTT	3615	0.0	40.829872	34
CACTTAC	3665	0.0	40.518417	36
GCGATAT	50	1.0822987E-9	40.500004	8
CCGCACT	3685	0.0	40.23745	33
GCACTTA	3680	0.0	40.23098	35
CATGCGG	560	0.0	40.178574	1
ACCCGCA	3730	0.0	40.11394	31
ACTTACT	3685	0.0	40.054276	37
ACCGGTA	135	0.0	40.0	41
CGGTCTA	310	0.0	39.919353	30
>>END_MODULE