FastQCFastQC Report
Thu 26 May 2016
SRR1043326_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043326_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1626286
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT657474.042769844910428No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC100820.6199401581271683No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT68950.42397216725717374No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTT66750.41044441137659676Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA55960.3440969177623124No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT55160.33917773380573896No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC26780.16466968294629603No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC26710.16423925435009584No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA25580.15729090701143586No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG21110.12980496665408175No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC20610.12673047668122334No Hit
GCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC16830.10348733248641384No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT16410.10090476090921277No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA206.3122314E-446.021
TATACGG405.6134013E-946.01
ATCGAAT206.3122314E-446.013
ACTACGG1350.045.9999961
CGTTTTT152950.045.0225561
ACGGGTA1200.044.0833324
TAACCGG650.042.4615361
TACGCGG1900.042.368421
TACGGGT1250.042.323
TAGTAGG3500.042.0571441
TTCGAAT1700.041.94117715
CTCGAGG2800.041.8928571
CTCACGA2800.041.07142623
TACGGGA3250.041.0461543
TAACGGG3700.041.0270232
TCGGCGT9200.041.04
CTTGCGG3650.040.9589041
TAGGGCG4500.040.888894
ATAACGG451.5890691E-840.888891
TAATCGT900.040.8888920
Produced by FastQC (version 0.11.5)