##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043326_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1626286
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30108849242999	33.0	31.0	34.0	31.0	34.0
2	32.637096427073715	34.0	31.0	34.0	31.0	34.0
3	32.77815587172244	34.0	31.0	34.0	31.0	34.0
4	36.212543796109664	37.0	37.0	37.0	35.0	37.0
5	36.14782393748701	37.0	37.0	37.0	35.0	37.0
6	36.28902542357248	37.0	37.0	37.0	35.0	37.0
7	36.215387084436564	37.0	37.0	37.0	35.0	37.0
8	36.19857392857099	37.0	37.0	37.0	35.0	37.0
9	37.78570681909578	39.0	38.0	39.0	35.0	39.0
10	37.640956756683636	39.0	37.0	39.0	35.0	39.0
11	37.408970500883605	39.0	37.0	39.0	35.0	39.0
12	37.42192455693525	39.0	37.0	39.0	35.0	39.0
13	37.43106317093057	39.0	37.0	39.0	35.0	39.0
14	38.81481609015881	40.0	38.0	41.0	35.0	41.0
15	38.87835042544792	41.0	38.0	41.0	35.0	41.0
16	38.819911134941826	41.0	38.0	41.0	35.0	41.0
17	38.807209187067954	41.0	38.0	41.0	35.0	41.0
18	38.5965217680039	40.0	38.0	41.0	35.0	41.0
19	38.37445074236635	40.0	37.0	41.0	35.0	41.0
20	38.09521387996945	40.0	35.0	41.0	34.0	41.0
21	38.04384960578889	40.0	35.0	41.0	34.0	41.0
22	37.98935119653001	40.0	35.0	41.0	34.0	41.0
23	37.9894544993931	40.0	35.0	41.0	34.0	41.0
24	37.89658645527293	40.0	35.0	41.0	34.0	41.0
25	37.79434982530748	40.0	35.0	41.0	34.0	41.0
26	37.71070402130991	40.0	35.0	41.0	34.0	41.0
27	37.74608832640753	40.0	35.0	41.0	34.0	41.0
28	37.66924882831187	40.0	35.0	41.0	34.0	41.0
29	37.63818725611608	40.0	35.0	41.0	34.0	41.0
30	37.573677692607575	40.0	35.0	41.0	33.0	41.0
31	37.460366134861886	40.0	35.0	41.0	33.0	41.0
32	37.26152103627529	40.0	35.0	41.0	33.0	41.0
33	36.9802679233542	40.0	35.0	41.0	32.0	41.0
34	36.80323879071701	40.0	35.0	41.0	32.0	41.0
35	36.64897318183886	40.0	35.0	41.0	31.0	41.0
36	36.570574917326965	39.0	35.0	41.0	31.0	41.0
37	36.48215012611558	39.0	35.0	41.0	31.0	41.0
38	36.4077370155065	39.0	35.0	41.0	31.0	41.0
39	36.32528226892441	39.0	35.0	41.0	31.0	41.0
40	36.21732524291545	39.0	35.0	41.0	31.0	41.0
41	36.171752692945766	39.0	35.0	41.0	31.0	41.0
42	36.069095472752025	39.0	35.0	41.0	31.0	41.0
43	35.948146267015765	38.0	35.0	41.0	30.0	41.0
44	35.77565139219055	38.0	35.0	41.0	30.0	41.0
45	35.679159139290384	38.0	35.0	41.0	29.0	41.0
46	35.63746475097246	38.0	35.0	40.0	29.0	41.0
47	35.52059846792016	37.0	35.0	40.0	29.0	41.0
48	35.413985608927334	37.0	35.0	40.0	29.0	41.0
49	35.33998079058665	37.0	35.0	40.0	29.0	41.0
50	35.17548204928284	37.0	35.0	40.0	28.0	41.0
51	34.95516225313383	36.0	35.0	40.0	28.0	41.0
52	34.50281992220311	36.0	34.0	39.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	1.0
13	6.0
14	7.0
15	31.0
16	52.0
17	103.0
18	283.0
19	574.0
20	1127.0
21	1828.0
22	2853.0
23	4660.0
24	7979.0
25	13318.0
26	19400.0
27	23163.0
28	24175.0
29	24572.0
30	25084.0
31	28916.0
32	35404.0
33	45796.0
34	91197.0
35	146166.0
36	146806.0
37	155692.0
38	244885.0
39	581226.0
40	979.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.485429992018624	16.899118605214582	22.57468858491065	28.040762817856148
2	34.59182456222337	19.82345048779858	24.215236434427894	21.369488515550156
3	27.135325520849346	20.614086329218846	32.48069527746042	19.769892872471388
4	23.510809291846577	24.056223813031657	30.41611377088655	22.01685312423522
5	21.891290953743685	32.26277542818422	27.574055239976243	18.271878378095856
6	78.23267248196196	2.266452518191757	16.426938435182986	3.073936564663288
7	77.6724389191077	2.281025600663106	14.997239108004374	5.049296372224812
8	67.23491440005017	4.117479951250886	21.221790017254037	7.4258156314449
9	37.612326491158385	17.334158936374045	25.71441923499311	19.339095337474465
10	25.36995337843405	17.59954891083118	36.71783437845495	20.312663332279808
11	22.60961479100232	15.068997703970888	39.11181674072088	23.20957076430591
12	20.0110558659424	16.707454900306587	42.369976744557846	20.911512489193168
13	16.66004626492511	21.184711668181365	41.491410489913825	20.663831576979693
14	15.155452362007665	19.696965970315183	42.594721961573796	22.552859706103355
15	17.390668062075182	16.180118380161915	43.387755905172895	23.04145765259001
16	17.750567858297988	19.867046755613714	37.830246340434584	24.552139045653718
17	18.032683058207475	20.582849511094604	38.125766316625736	23.258701114072185
18	20.563910652861797	24.869549390451617	35.063820262856595	19.502719693829988
19	21.64336408233238	20.13132991368062	33.89606748136551	24.329238522621484
20	20.03423752033775	24.67142925660062	35.49984443080738	19.794488792254253
21	19.23351735180651	19.81453446687729	34.975643890435016	25.976304290881185
22	18.57815906919201	22.816343496777318	32.775661845456455	25.829835588574213
23	18.522018882287618	20.892266181963073	38.00524630968968	22.580468626059623
24	22.663664324725172	19.540166981699407	34.86846716998117	22.92770152359425
25	17.54549937710833	22.302042814117566	35.449607264650865	24.702850544123237
26	18.545077557084056	20.812636891666042	34.90892745802399	25.733358093225917
27	22.57358176852042	21.294962878608068	35.37901697487404	20.75243837799747
28	20.049671460001502	22.8380493959857	33.204245747672914	23.90803339633988
29	22.04532290138389	20.884887406028213	33.90732011466618	23.16246957792172
30	22.765614412225155	23.370551059284775	30.627884640217033	23.235949888273034
31	23.937056581683663	23.37073552868315	29.61207315318462	23.08013473644857
32	23.189832538680157	22.123046007897752	30.469978835211027	24.217142618211067
33	19.975945190452357	24.61608843708917	31.000820273924756	24.407146098533712
34	20.270604309451105	25.57539079842045	31.755730541860412	22.398274350268036
35	21.989367183877867	23.569286091130344	30.259314782270767	24.18203194272102
36	22.53773321543689	24.2659040291806	29.697236525432796	23.499126229949713
37	19.97151792489144	26.28990226811274	30.47901783573123	23.259561971264585
38	20.314016107867864	23.01636981441149	28.666298547733916	28.003315529986732
39	23.91147682510948	20.247853083651954	32.86463758527098	22.976032505967588
40	20.588814021641948	20.416642583161877	34.09375718662031	24.90078620857586
41	21.990043571671897	25.34283637687344	28.5280694785542	24.139050572900462
42	21.088172683033612	20.7067514570008	32.4646464398021	25.74042942016349
43	23.122808657271847	21.49056193068132	32.88868009685873	22.497949315188105
44	20.37519845832775	22.61619419954424	33.29611150806193	23.712495834066086
45	21.83533523623766	22.04722908516706	31.479641342297725	24.637794336297553
46	23.32160517891687	21.809632500064563	30.81167765079451	24.057084670224057
47	20.159615221430915	22.55083054272127	33.195083767553804	24.094470468294016
48	21.651911164456926	20.238814083131746	30.648176274037898	27.461098478373426
49	20.596623226173012	19.171412654354768	35.172411248697955	25.059552870774265
50	20.428694583855485	19.454634670654485	35.052690609154844	25.063980136335186
51	19.652570335107107	17.83585421014508	34.27675082980484	28.234824624942966
52	18.11095957291645	19.328519091967834	37.65850533055071	24.902016004565002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	242.0
1	3057.0
2	5872.0
3	43698.5
4	81525.0
5	46472.0
6	11419.0
7	10699.5
8	9980.0
9	9769.0
10	9558.0
11	9409.0
12	9260.0
13	8979.0
14	8374.0
15	8050.0
16	7622.0
17	7194.0
18	6892.0
19	6590.0
20	6294.5
21	5999.0
22	5880.5
23	5762.0
24	5999.0
25	6236.0
26	7259.5
27	8283.0
28	9284.0
29	10285.0
30	11310.5
31	12336.0
32	15046.5
33	17757.0
34	18797.0
35	19837.0
36	22054.0
37	24271.0
38	27150.5
39	32017.5
40	34005.0
41	40904.5
42	47804.0
43	59914.0
44	72024.0
45	88144.0
46	104264.0
47	130837.5
48	157411.0
49	170680.0
50	183949.0
51	169575.5
52	155202.0
53	130604.0
54	106006.0
55	94814.0
56	83622.0
57	77363.5
58	71105.0
59	67037.5
60	62970.0
61	60274.0
62	57578.0
63	56112.0
64	47303.5
65	39961.0
66	34095.0
67	28229.0
68	23682.0
69	19135.0
70	17242.5
71	15350.0
72	15677.5
73	16005.0
74	12360.0
75	8715.0
76	6358.0
77	4001.0
78	3084.0
79	2167.0
80	1711.5
81	1256.0
82	1069.5
83	883.0
84	625.5
85	368.0
86	326.0
87	284.0
88	185.0
89	51.5
90	17.0
91	25.0
92	33.0
93	20.0
94	7.0
95	12.0
96	17.0
97	9.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1626286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.372557106039814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.74170928098465	20.638683005656837
2	11.390400967964865	6.463496038485486
3	4.539010067031641	3.863499669952345
4	2.1689179499214073	2.4615099356983974
5	1.2651256581552237	1.7947424991162642
6	0.8226191093500989	1.4003884593933176
7	0.5941737293759736	1.1800759647339918
8	0.4553321722631358	1.0335150447802384
9	0.3587746378969422	0.9161418511736775
>10	4.939474633087886	33.45453373768791
>50	0.5816962477292497	10.809654397269897
>100	0.1303416757466571	6.498931580216907
>500	0.006756841846738146	1.2942823140814008
>1k	0.0043592528043471905	1.9747870006001897
>5k	8.718505608694383E-4	1.5263737434257314
>10k+	4.3592528043471916E-4	4.689384757727485
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65747	4.042769844910428	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	10082	0.6199401581271683	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	6895	0.42397216725717374	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTT	6675	0.41044441137659676	Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	5596	0.3440969177623124	No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	5516	0.33917773380573896	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2678	0.16466968294629603	No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	2671	0.16423925435009584	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	2558	0.15729090701143586	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG	2111	0.12980496665408175	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2061	0.12673047668122334	No Hit
GCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	1683	0.10348733248641384	No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	1641	0.10090476090921277	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.420774697685401	0.0
2	0.0	0.0	0.0	1.3716529564910478	0.0
3	0.0	0.0	0.0	2.149621899223138	0.0
4	0.0	0.0	0.0	2.7185255238008565	0.0
5	0.0	0.0	0.0	5.459802273400865	0.0
6	0.0	0.0	0.0	6.121186556362165	0.0
7	0.0	0.0	0.0	8.510987612265001	0.0
8	0.0	0.0	0.0	11.083720821552912	0.0
9	0.0	0.0	0.0	13.385468484633083	0.0
10	0.0	0.0	0.0	14.731910623346693	0.0
11	0.0	0.0	0.0	16.018092758592278	0.0
12	0.0	0.0	0.0	16.99116883500196	0.0
13	0.0	0.0	0.0	17.438261166855032	0.0
14	0.0	0.0	0.0	17.743926959956614	0.0
15	6.148979945716805E-5	0.0	0.0	18.785379693362668	0.0
16	6.148979945716805E-5	0.0	0.0	19.87141253137517	0.0
17	6.148979945716805E-5	0.0	0.0	21.199715179248916	0.0
18	6.148979945716805E-5	0.0	0.0	21.897747382686685	0.0
19	6.148979945716805E-5	0.0	0.0	22.681434876768293	0.0
20	6.148979945716805E-5	0.0	0.0	23.56824076453957	0.0
21	1.229795989143361E-4	0.0	0.0	24.394540689644995	0.0
22	3.689387967430083E-4	0.0	0.0	25.23258516644674	0.0
23	3.689387967430083E-4	0.0	0.0	25.946789187141746	0.0
24	8.608571924003527E-4	0.0	0.0	26.491404340933883	0.0
25	8.608571924003527E-4	0.0	0.0	27.032391596558046	0.0
26	8.608571924003527E-4	0.0	0.0	27.560343014697292	0.0
27	8.608571924003527E-4	0.0	0.0	28.144742068738218	0.0
28	9.223469918575208E-4	0.0	0.0	28.75656557333704	0.0
29	9.223469918575208E-4	0.0	0.0	29.366544383952146	0.0
30	9.223469918575208E-4	0.0	0.0	29.95494027495779	0.0
31	9.223469918575208E-4	0.0	0.0	30.497772224565665	0.0
32	9.223469918575208E-4	0.0	0.0	31.069012461522757	0.0
33	9.223469918575208E-4	0.0	0.0	31.595918553071233	0.0
34	9.223469918575208E-4	0.0	0.0	32.212845711025	0.0
35	9.223469918575208E-4	0.0	0.0	32.725301699701035	0.0
36	9.223469918575208E-4	0.0	0.0	33.26481320013823	0.0
37	9.223469918575208E-4	0.0	0.0	33.80776812934502	0.0
38	9.223469918575208E-4	0.0	0.0	34.392105693586494	0.0
39	0.001106816390229025	0.0	0.0	35.02815617917144	0.0
40	0.001106816390229025	0.0	0.0	35.62306998891954	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTA	20	6.3122314E-4	46.0	21
TATACGG	40	5.6134013E-9	46.0	1
ATCGAAT	20	6.3122314E-4	46.0	13
ACTACGG	135	0.0	45.999996	1
CGTTTTT	15295	0.0	45.022556	1
ACGGGTA	120	0.0	44.083332	4
TAACCGG	65	0.0	42.461536	1
TACGCGG	190	0.0	42.36842	1
TACGGGT	125	0.0	42.32	3
TAGTAGG	350	0.0	42.057144	1
TTCGAAT	170	0.0	41.941177	15
CTCGAGG	280	0.0	41.892857	1
CTCACGA	280	0.0	41.071426	23
TACGGGA	325	0.0	41.046154	3
TAACGGG	370	0.0	41.027023	2
TCGGCGT	920	0.0	41.0	4
CTTGCGG	365	0.0	40.958904	1
TAGGGCG	450	0.0	40.88889	4
ATAACGG	45	1.5890691E-8	40.88889	1
TAATCGT	90	0.0	40.88889	20
>>END_MODULE