##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043325_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1584187
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29058627548389	33.0	31.0	34.0	31.0	34.0
2	32.58031217274223	34.0	31.0	34.0	31.0	34.0
3	32.70048106694475	34.0	31.0	34.0	31.0	34.0
4	36.18917905525042	37.0	37.0	37.0	35.0	37.0
5	36.1686606442295	37.0	37.0	37.0	35.0	37.0
6	36.313494555882606	37.0	37.0	37.0	35.0	37.0
7	36.25112628749005	37.0	37.0	37.0	35.0	37.0
8	36.15635969743471	37.0	37.0	37.0	35.0	37.0
9	37.74987675066138	39.0	38.0	39.0	35.0	39.0
10	37.59334220013167	39.0	37.0	39.0	35.0	39.0
11	37.402734020668014	39.0	37.0	39.0	35.0	39.0
12	37.424513015193284	39.0	37.0	39.0	35.0	39.0
13	37.44451317931532	39.0	37.0	39.0	35.0	39.0
14	38.824924077776174	40.0	38.0	41.0	35.0	41.0
15	38.8990535839519	41.0	38.0	41.0	35.0	41.0
16	38.880784276098716	41.0	38.0	41.0	35.0	41.0
17	38.84264988918606	41.0	38.0	41.0	35.0	41.0
18	38.65699882652742	40.0	38.0	41.0	35.0	41.0
19	38.46117093499694	40.0	37.0	41.0	35.0	41.0
20	38.20352963381217	40.0	35.0	41.0	34.0	41.0
21	38.153635271593565	40.0	35.0	41.0	34.0	41.0
22	38.115054598983576	40.0	35.0	41.0	34.0	41.0
23	38.074157911913176	40.0	35.0	41.0	34.0	41.0
24	37.98861876786011	40.0	35.0	41.0	34.0	41.0
25	37.95150446254135	40.0	35.0	41.0	34.0	41.0
26	37.92046077893582	40.0	35.0	41.0	34.0	41.0
27	37.90529779628289	40.0	35.0	41.0	34.0	41.0
28	37.79358560573973	40.0	35.0	41.0	34.0	41.0
29	37.77843082918873	40.0	35.0	41.0	34.0	41.0
30	37.656507722888776	40.0	35.0	41.0	34.0	41.0
31	37.533239447110724	40.0	35.0	41.0	33.0	41.0
32	37.35206134124317	40.0	35.0	41.0	33.0	41.0
33	37.045564696592	40.0	35.0	41.0	33.0	41.0
34	36.82765481600342	40.0	35.0	41.0	32.0	41.0
35	36.675252353415345	39.0	35.0	41.0	31.0	41.0
36	36.578994146524366	39.0	35.0	41.0	31.0	41.0
37	36.439184894207564	39.0	35.0	41.0	31.0	41.0
38	36.35536903156004	39.0	35.0	41.0	31.0	41.0
39	36.23276355632258	39.0	35.0	41.0	31.0	41.0
40	36.096440634849294	39.0	35.0	41.0	30.0	41.0
41	36.01221762329826	39.0	35.0	41.0	30.0	41.0
42	35.89765917786221	38.0	35.0	41.0	30.0	41.0
43	35.76552768075991	38.0	35.0	40.0	29.0	41.0
44	35.634184600681614	38.0	35.0	40.0	29.0	41.0
45	35.57122359923418	38.0	35.0	40.0	29.0	41.0
46	35.4698157477621	38.0	35.0	40.0	29.0	41.0
47	35.30144547329324	37.0	35.0	40.0	28.0	41.0
48	35.22156917081127	37.0	35.0	40.0	28.0	41.0
49	35.13097633044584	37.0	35.0	40.0	28.0	41.0
50	35.00106553077383	36.0	35.0	40.0	28.0	41.0
51	34.481703864505896	36.0	34.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	1.0
13	7.0
14	15.0
15	15.0
16	49.0
17	108.0
18	254.0
19	539.0
20	979.0
21	1705.0
22	2560.0
23	4005.0
24	6502.0
25	11462.0
26	17763.0
27	23021.0
28	24070.0
29	24293.0
30	25040.0
31	28004.0
32	33209.0
33	43987.0
34	88592.0
35	143436.0
36	139999.0
37	152099.0
38	250815.0
39	561578.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.72662886389044	16.991175915469576	22.553776795289952	27.72841842535004
2	34.4379167358399	19.824237921407008	24.27270265442148	21.465142688331618
3	27.201902300675364	20.66233342402128	32.21803991574227	19.917724359561088
4	23.700042987349345	24.109590597574655	30.110649815962383	22.079716599113613
5	22.043609750616564	32.44768452209241	27.227783083688983	18.28092264360205
6	78.58832322194287	2.2457576031112487	16.136794456715023	3.0291247182308654
7	78.05732530313657	2.2687346885184643	14.63596153736901	5.037978470975965
8	67.61815366493981	4.033362223020388	20.92076251099144	7.42772160104836
9	37.77982018536953	17.401670383610014	25.406280950418104	19.41222848060235
10	25.610423516920665	17.591610081385596	36.30373181953898	20.494234582154757
11	22.81454146511744	14.987308947744173	39.01717410886467	23.180975478273712
12	20.237509839431837	16.668107994826368	42.16661290617837	20.927769259563423
13	16.87793170881973	21.164546862207555	41.3378597349934	20.61966169397931
14	15.287841650007227	19.640294990427268	42.42384263978936	22.648020719776138
15	17.48240580184031	16.146010540422314	43.2837789983127	23.087804659424677
16	17.840949332370485	19.67293002656883	37.77855770814935	24.70756293291133
17	18.279533918659855	20.39348889998466	37.98692957333951	23.340047608015972
18	20.755251747426282	24.799029407513128	34.96929339781225	19.476425447248335
19	21.810745827354978	20.147810832938283	33.64306107801667	24.398382261690067
20	20.178552153249584	24.643555337848372	35.32455448756996	19.85333802133208
21	19.395563781296023	19.80668948804655	34.74823363655932	26.04951309409811
22	18.662001392512373	22.756720008433348	32.533154229898365	26.04812436915591
23	18.692616465101658	20.911041436396083	37.690310550459	22.706031548043253
24	22.75974995376177	19.4804653743529	34.69704018528116	23.06274448660417
25	17.475840920295393	22.28922469380193	35.37461170934997	24.860322676552705
26	18.59843566447648	20.74944435221347	34.77682874559632	25.875291237713725
27	22.58281377135401	21.186008974950557	35.31312906872736	20.918048184968065
28	20.056218110614466	22.95234085369972	33.04104881557544	23.950392220110377
29	22.112162263672154	20.952198193773842	33.62273519477183	23.312904347782172
30	22.911436591765995	23.284182991023155	30.444890660004155	23.359489757206696
31	24.03933374027182	23.306402590098266	29.389585951658486	23.264677717971427
32	23.399510285086293	22.055918903513287	30.217518512650337	24.327052298750086
33	20.09554427602297	24.53542416394024	30.85753133941889	24.511500220617894
34	20.456170893966434	25.517378945793645	31.520142508428613	22.50630765181131
35	22.11550782830562	23.54097085760709	30.20337876778436	24.140142546302933
36	22.591398616451215	24.214123711405282	29.572518900862082	23.62195877128142
37	20.09346118860968	26.29108811017891	30.274329987558286	23.341120713653122
38	20.478642988485575	22.872741664967585	28.51254302680176	28.136072319745082
39	24.047287346758935	20.253480176267068	32.54331717152078	23.155915305453206
40	20.63361206726226	20.31452094986261	33.98683362507078	25.06503335780435
41	22.218652217194055	25.2440526276254	28.307390478523054	24.22990467665749
42	21.214288464682515	20.664227139851548	32.372377755908865	25.749106639557073
43	23.233936397660123	21.31686473882187	32.74947970157564	22.699719161942372
44	20.53191952717703	22.553145556679862	33.20346650995116	23.711468406191948
45	21.98162211910589	21.984020825824224	31.40412085189438	24.63023620317551
46	23.43555400972234	21.790924934998205	30.733682324119567	24.039838731159897
47	20.382063481142062	22.379302443461533	33.08245806839723	24.156176006999175
48	21.87891959724452	20.049211362042485	30.550875622637985	27.52099341807501
49	20.79596663777698	19.089349931542174	34.96727343425997	25.147409996420876
50	20.56985696764334	19.33338677820232	34.962476020823296	25.13428023333104
51	19.872275179634727	17.920927264268673	33.961268461362195	28.245529094734394
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	233.0
1	2922.0
2	5611.0
3	41471.0
4	77331.0
5	44404.0
6	11477.0
7	10572.0
8	9667.0
9	9498.0
10	9329.0
11	9087.0
12	8845.0
13	8603.0
14	8361.0
15	7857.0
16	7353.0
17	7057.0
18	6761.0
19	6417.5
20	6074.0
21	5825.0
22	5576.0
23	5415.0
24	5254.0
25	5665.0
26	7004.0
27	7932.0
28	9239.0
29	10546.0
30	11904.0
31	13262.0
32	15473.5
33	17685.0
34	19463.5
35	21242.0
36	23093.5
37	24945.0
38	28611.0
39	32277.0
40	36156.5
41	40036.0
42	47815.5
43	55595.0
44	66344.0
45	77093.0
46	96821.0
47	116549.0
48	147377.5
49	178206.0
50	182611.5
51	187017.0
52	156960.0
53	126903.0
54	109147.0
55	91391.0
56	83168.0
57	74945.0
58	71373.0
59	67801.0
60	64431.5
61	61062.0
62	56949.5
63	52837.0
64	50613.5
65	48390.0
66	40935.0
67	33480.0
68	28127.0
69	22774.0
70	19839.5
71	16905.0
72	16388.5
73	15872.0
74	13147.0
75	7778.5
76	5135.0
77	3862.0
78	2589.0
79	1928.5
80	1268.0
81	1185.0
82	1102.0
83	753.5
84	405.0
85	388.5
86	372.0
87	240.5
88	109.0
89	67.5
90	26.0
91	27.0
92	28.0
93	24.0
94	20.0
95	19.0
96	18.0
97	9.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1584187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.1423899765234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.3633536703992	20.364777190014603
2	11.425671552342804	6.430914091394015
3	4.481318030438297	3.78344998864261
4	2.2161317715835427	2.49468978221071
5	1.3300486520121622	1.8715373926337768
6	0.8703968511828342	1.4697028572195192
7	0.6252324705873399	1.2316875209277882
8	0.4727227368860105	1.064283808977246
9	0.36935791829718456	0.9355153119872613
>10	5.12907608619378	34.268590767670595
>50	0.5802674571492635	10.631687886004729
>100	0.1244121581497414	6.275665460031235
>500	0.006571862236953531	1.2846864475581579
>1k	0.004305702844900589	2.142589649134426
>5k	9.064637568211767E-4	1.7807932721984931
>10k+	2.2661593920529418E-4	3.9694285733949224
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62241	3.92889223305077	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	9766	0.6164676266122623	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	6472	0.40853762844916663	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	6421	0.4053183115377162	No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	5264	0.3322840043504965	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	4697	0.29649277515848826	No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG	2735	0.17264375985915803	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	2484	0.15679967074594098	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2431	0.15345410611247282	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2037	0.12858330487499264	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC	1967	0.12416463460437435	No Hit
GCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG	1740	0.1098355181553693	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.42040491431882726	0.0
2	0.0	0.0	0.0	1.3425813997968674	0.0
3	0.0	0.0	0.0	2.1375001814811005	0.0
4	0.0	0.0	0.0	2.716030367627054	0.0
5	0.0	0.0	0.0	5.435153804443541	0.0
6	0.0	0.0	0.0	6.087223288664785	0.0
7	0.0	0.0	0.0	8.463079169315238	0.0
8	0.0	0.0	0.0	11.076659510524957	0.0
9	0.0	0.0	0.0	13.426129617273718	0.0
10	0.0	0.0	0.0	14.832971107577578	0.0
11	0.0	0.0	0.0	16.13969815432143	0.0
12	0.0	0.0	0.0	17.12670284505554	0.0
13	0.0	0.0	0.0	17.593251301771822	0.0
14	0.0	0.0	0.0	17.904767555850412	0.0
15	0.0	0.0	0.0	18.97812568844461	0.0
16	0.0	0.0	0.0	20.08039454938085	0.0
17	0.0	0.0	0.0	21.459398416979813	0.0
18	0.0	0.0	0.0	22.17307678954568	0.0
19	0.0	0.0	0.0	22.97885287532343	0.0
20	6.312386100883293E-5	0.0	0.0	23.893770116785454	0.0
21	6.312386100883293E-5	0.0	0.0	24.719998333530068	0.0
22	3.7874316605299754E-4	0.0	0.0	25.57570539336581	0.0
23	3.7874316605299754E-4	0.0	0.0	26.30036731774721	0.0
24	5.681147490794963E-4	0.0	0.0	26.859329106980425	0.0
25	6.312386100883293E-4	0.0	0.0	27.417975276908596	0.0
26	6.312386100883293E-4	0.0	0.0	27.95938863278136	0.0
27	6.943624710971622E-4	0.0	0.0	28.5514273251832	0.0
28	6.943624710971622E-4	0.0	0.0	29.159878221447343	0.0
29	6.943624710971622E-4	0.0	0.0	29.76365795199683	0.0
30	6.943624710971622E-4	0.0	0.0	30.357842855672974	0.0
31	6.943624710971622E-4	0.0	0.0	30.91017663950026	0.0
32	6.943624710971622E-4	0.0	0.0	31.494072353831964	0.0
33	6.943624710971622E-4	0.0	0.0	32.03416010862354	0.0
34	6.943624710971622E-4	0.0	0.0	32.66445186079674	0.0
35	6.943624710971622E-4	0.0	0.0	33.174618905470126	0.0
36	6.943624710971622E-4	0.0	0.0	33.729730139181804	0.0
37	6.943624710971622E-4	0.0	0.0	34.269249779224296	0.0
38	6.943624710971622E-4	0.0	0.0	34.853902979888105	0.0
39	6.943624710971622E-4	0.0	0.0	35.489939003413106	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	40	6.8175723E-9	45.000004	8
TACGCTA	25	3.8914768E-5	45.0	37
ACGCTAT	25	3.8914768E-5	45.0	38
GCGATAA	25	3.8914768E-5	45.0	8
CCGGTTA	25	3.8914768E-5	45.0	29
CGATACG	25	3.8914768E-5	45.0	9
CGTTTTT	14605	0.0	43.937008	1
ACGGGAT	165	0.0	43.636364	4
ACTACGG	120	0.0	43.124996	1
TAGTAGG	315	0.0	42.142857	1
ATAACGG	65	0.0	41.538464	1
ACGTAGG	125	0.0	41.399998	1
TACGGGA	305	0.0	41.311478	3
TAACGGG	415	0.0	41.20482	2
TACGCGG	170	0.0	41.02941	1
ACGATAC	55	6.184564E-11	40.90909	44
AGGGTAC	545	0.0	40.458714	5
CGTAGGG	685	0.0	40.401463	2
ATCGCCA	95	0.0	40.26316	23
GTTGATC	1040	0.0	40.240387	15
>>END_MODULE