FastQCFastQC Report
Thu 26 May 2016
SRR1043324_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043324_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2256627
Sequences flagged as poor quality0
Sequence length52
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1105874.9005440420592326No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCTT225020.99715194402974Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT201690.8937675566232258No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT120310.5331408336424229No Hit
CGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC109020.48311041213279815No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCG102070.45231223414414523No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT81780.36239927998734395No Hit
TCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT67450.2988974252280062No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC61530.2726635815311968No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT56760.25152583922819327No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCT52350.23198339823107672No Hit
CGTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG50650.22445003095327676No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCC43360.19214517950906373No Hit
ACCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC42280.18735927559140256No Hit
GCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC39680.1758376550488849No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGT37370.16560113833610962No Hit
ACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT35860.15890973563641664No Hit
TCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC34750.15399088994326487No Hit
AGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC31840.14109553772067782No Hit
TGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCTTG30760.1363096338030166Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTC30410.1347586464222931No Hit
TGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC26640.11805229663564248No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGTA206.312753E-446.03
TCCGTAC206.312753E-446.046
CGTTTTT306800.045.670141
ATTGCGG3700.044.7567561
TGAACGG1600.044.56251
TACGCGG2650.044.2641531
CCCGCAC1250.044.1632
ACCCGCA1200.044.08333231
AGTACGG1150.044.01
TCTACGG2250.043.9555551
GTAGCGG3700.043.5135151
AATGCGG2450.043.1836741
ACGTAGG1550.043.0322571
GCGAAGG3600.042.8055571
ACTGCGG2850.042.771931
CCGCACT1250.042.3233
GCGATAC1200.042.1666688
GCGTAGG4150.042.120481
CTAGCGG4050.042.0246931
TAAGCGG4600.042.01
Produced by FastQC (version 0.11.5)