##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043324_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2256627
Sequences flagged as poor quality	0
Sequence length	52
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29278742122646	33.0	31.0	34.0	31.0	34.0
2	32.62308923893936	34.0	31.0	34.0	31.0	34.0
3	32.691633132103796	34.0	31.0	34.0	31.0	34.0
4	36.2015720808091	37.0	35.0	37.0	35.0	37.0
5	36.098193011073604	37.0	35.0	37.0	35.0	37.0
6	36.34123539246849	37.0	37.0	37.0	35.0	37.0
7	36.26932142529537	37.0	37.0	37.0	35.0	37.0
8	36.2456068282441	37.0	37.0	37.0	35.0	37.0
9	37.82654510470716	39.0	38.0	39.0	35.0	39.0
10	37.60846785933165	39.0	37.0	39.0	35.0	39.0
11	37.49455714214179	39.0	37.0	39.0	35.0	39.0
12	37.55445849048159	39.0	37.0	39.0	35.0	39.0
13	37.54370438712291	39.0	37.0	39.0	35.0	39.0
14	38.91738156106437	40.0	38.0	41.0	35.0	41.0
15	38.99257298614259	41.0	38.0	41.0	35.0	41.0
16	39.023840005459476	41.0	38.0	41.0	35.0	41.0
17	38.98412453631016	41.0	38.0	41.0	35.0	41.0
18	38.744883403415805	40.0	38.0	41.0	35.0	41.0
19	38.503635735990045	40.0	37.0	41.0	35.0	41.0
20	38.14980942796483	40.0	35.0	41.0	34.0	41.0
21	38.122901569466286	40.0	35.0	41.0	34.0	41.0
22	38.09971873951699	40.0	35.0	41.0	34.0	41.0
23	38.09437669583853	40.0	35.0	41.0	34.0	41.0
24	38.02472717024125	40.0	35.0	41.0	34.0	41.0
25	37.95561384313845	40.0	35.0	41.0	34.0	41.0
26	37.9060500472608	40.0	35.0	41.0	34.0	41.0
27	37.93488467522546	40.0	35.0	41.0	34.0	41.0
28	37.92934853655478	40.0	35.0	41.0	34.0	41.0
29	37.86758866219362	40.0	35.0	41.0	34.0	41.0
30	37.76119934752177	40.0	35.0	41.0	34.0	41.0
31	37.57788416074079	40.0	35.0	41.0	33.0	41.0
32	37.354155560489176	40.0	35.0	41.0	33.0	41.0
33	36.959949074437205	40.0	35.0	41.0	32.0	41.0
34	36.731929999951255	40.0	35.0	41.0	31.0	41.0
35	36.59120049525243	40.0	35.0	41.0	30.0	41.0
36	36.49415078344804	40.0	35.0	41.0	30.0	41.0
37	36.431606109472234	40.0	35.0	41.0	30.0	41.0
38	36.39019829152093	40.0	35.0	41.0	30.0	41.0
39	36.41326546212555	40.0	35.0	41.0	30.0	41.0
40	36.39726591944526	40.0	35.0	41.0	30.0	41.0
41	36.36041977695029	40.0	35.0	41.0	30.0	41.0
42	36.281581315831104	40.0	35.0	41.0	30.0	41.0
43	36.18536160384503	40.0	35.0	41.0	30.0	41.0
44	36.04419649326184	39.0	35.0	41.0	29.0	41.0
45	35.967345068547	39.0	35.0	41.0	28.0	41.0
46	35.9466287516723	39.0	35.0	41.0	28.0	41.0
47	35.87772591571403	39.0	35.0	41.0	28.0	41.0
48	35.826653230684556	39.0	35.0	41.0	27.0	41.0
49	35.80630161741396	39.0	35.0	41.0	27.0	41.0
50	35.708229140216794	39.0	35.0	41.0	27.0	41.0
51	35.62726272441125	39.0	35.0	41.0	26.0	41.0
52	35.272115418276925	38.0	35.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	2.0
14	4.0
15	17.0
16	71.0
17	123.0
18	338.0
19	680.0
20	1299.0
21	2334.0
22	3929.0
23	6912.0
24	12972.0
25	24626.0
26	35105.0
27	36602.0
28	33766.0
29	31823.0
30	32836.0
31	37903.0
32	46063.0
33	59760.0
34	101574.0
35	154078.0
36	201182.0
37	178531.0
38	290978.0
39	960724.0
40	2393.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.93561098045889	18.263053663720232	25.742313638895574	30.059021716925304
2	34.952475530958374	18.89031727440999	25.293901030165816	20.86330616446582
3	21.006307200968525	20.82368951536962	38.85923548730029	19.31076779636156
4	22.84081507488832	22.749129563724978	36.050175771184165	18.359879590202546
5	20.5339207587253	27.931997623001052	32.289075686854765	19.24500593141888
6	71.62264742910548	1.3836136853808805	22.664888791989103	4.328850093524538
7	71.89287374475268	1.2230643345134133	19.264016605314037	7.620045315419873
8	60.39185917743606	3.672250664376523	26.87280618374237	9.063083974445044
9	29.939728630385083	25.565102252166618	28.734212610236426	15.760956507211871
10	19.393767778192853	18.869090904256662	47.98861309378998	13.748528223760506
11	18.304044044496496	16.279916884801963	43.84317833651729	21.57286073418425
12	18.031513404740792	17.098217826871696	46.807646988181915	18.06262178020559
13	14.396397809651306	21.37047903796241	44.52658769038924	19.706535461997042
14	12.865263067401036	22.625803909994875	45.00748240626386	19.501450616340225
15	13.490399609682946	21.74413405494129	45.47920413962963	19.28626219574613
16	14.116732627944273	24.861485748420098	40.85912292993038	20.16265869370525
17	13.646295998408245	21.977358243076946	42.53706970624742	21.83927605226739
18	14.991312254971689	27.433953418088148	41.67423326938834	15.900501057551825
19	15.391555627048689	23.387959108882416	38.75824405185261	22.462241212216284
20	13.643326965422286	28.469259651683686	38.46289174063769	19.42452164225634
21	14.315126070901393	23.351533062397994	39.603975313598575	22.729365553102042
22	13.253851877160027	25.43291381340381	35.619577360370144	25.693656949066018
23	12.218988782816123	22.87936818978059	41.939452111492066	22.962190915911226
24	17.060240793006553	21.54560767020868	38.76644212800786	22.627709408776905
25	12.826798580359094	23.76994514379204	40.226541648220994	23.17671462762787
26	14.02354044332537	22.22538328221722	39.849518772929684	23.901557501527723
27	18.120805963945305	24.235906066886553	39.078810986485585	18.564476982682564
28	15.360358623733564	25.054916031758907	35.4803873214315	24.104338023076032
29	17.163669494338233	22.744476601582804	38.128011408176896	21.96384249590207
30	18.344192460694657	25.33125766907867	33.558315131388575	22.766234738838097
31	16.63026277714483	25.027042572831043	35.42663453020814	22.916060119815988
32	17.634460635275566	24.207899666183202	35.57801089856675	22.579628799974476
33	12.874613305610541	25.59962279986901	40.88057086970952	20.645193024810922
34	15.572267813865562	25.674291763769556	34.83650598880541	23.91693443355947
35	17.892190424026655	23.40634938782528	36.05132793323841	22.65013225490965
36	17.16065614742711	22.489715845817674	38.83512871201133	21.51449929474388
37	15.139276451092714	23.387560283555946	37.51058548887344	23.962577776477904
38	14.934280233286229	20.72172317356834	36.23367973528634	28.110316857859097
39	19.231977637420805	18.41177119656904	40.38474236105479	21.971508804955363
40	14.446118033684789	18.10631531041683	42.50675898143557	24.940807674462814
41	17.32949220230016	22.58281940258625	34.19271328402966	25.894975111083934
42	18.122224009550536	19.828753267598056	37.90081391386348	24.148208808987928
43	21.543170404324684	21.63512179903901	36.88084029837452	19.940867498261785
44	17.358828020758416	21.52579934566058	37.26929616635802	23.846076467222986
45	17.86560206892854	20.679093177561022	36.2083321700928	25.246972583417644
46	20.548012586927303	18.785116016071772	36.22720990221246	24.43966149478846
47	15.994180695347527	18.28122237303728	39.39406025009893	26.330536681516264
48	17.249638509155478	16.633320437981112	35.90114804085921	30.215893012004198
49	18.8652799066926	15.949911084109159	40.21338041244743	24.971428596750815
50	18.18213643637163	14.679962616772732	40.09927205515134	27.038628891704303
51	15.770218117571048	15.127710516625035	38.68893707289685	30.413134292907067
52	14.016361587448877	15.211197951633123	46.45118577416649	24.32125468675151
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	500.0
1	2253.0
2	4006.0
3	69645.5
4	135285.0
5	77052.5
6	18820.0
7	17938.5
8	17057.0
9	17487.0
10	17917.0
11	18135.5
12	18354.0
13	18018.0
14	16883.5
15	16085.0
16	15092.0
17	14099.0
18	13308.0
19	12517.0
20	12085.5
21	11654.0
22	11757.0
23	11860.0
24	12343.5
25	12827.0
26	13948.5
27	15070.0
28	17025.5
29	18981.0
30	20535.0
31	22089.0
32	24854.0
33	27619.0
34	31040.0
35	34461.0
36	39879.0
37	45297.0
38	49985.0
39	64865.5
40	75058.0
41	99785.5
42	124513.0
43	164769.0
44	205025.0
45	267503.0
46	329981.0
47	336542.5
48	343104.0
49	295018.5
50	246933.0
51	196252.0
52	145571.0
53	114058.0
54	82545.0
55	65869.5
56	49194.0
57	41720.5
58	34247.0
59	29553.0
60	24859.0
61	22716.0
62	20573.0
63	18639.0
64	14615.5
65	12526.0
66	10067.0
67	7608.0
68	5737.5
69	3867.0
70	3144.5
71	2422.0
72	2242.5
73	2063.0
74	1767.5
75	1472.0
76	1096.0
77	720.0
78	572.0
79	424.0
80	303.0
81	182.0
82	142.0
83	102.0
84	69.5
85	37.0
86	26.5
87	16.0
88	13.0
89	8.5
90	7.0
91	6.0
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2256627.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.53813975194442
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.92845854658675	24.12346694877367
2	12.731208955520646	8.539621303229154
3	5.252747878663579	5.2850217730904605
4	2.572822354610822	3.4515070274345776
5	1.4880567886173373	2.4953328267738932
6	1.012015518929681	2.0364670743000115
7	0.6835387800790013	1.6047233392514202
8	0.5170968155723338	1.3873972212760286
9	0.40352968799436795	1.2180271563012275
>10	3.0480159924382706	19.318264048480106
>50	0.1862002339531528	4.318684529260981
>100	0.15810704054667074	10.611165608323446
>500	0.012089985331085667	2.7056363136159898
>1k	0.0045171373764495894	2.9482533177930432
>5k	7.971418899616922E-4	1.6509541301686315
>10k+	7.971418899616922E-4	8.30547738192736
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	110587	4.9005440420592326	No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCTT	22502	0.99715194402974	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT	20169	0.8937675566232258	No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT	12031	0.5331408336424229	No Hit
CGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	10902	0.48311041213279815	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCG	10207	0.45231223414414523	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT	8178	0.36239927998734395	No Hit
TCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT	6745	0.2988974252280062	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC	6153	0.2726635815311968	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT	5676	0.25152583922819327	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCT	5235	0.23198339823107672	No Hit
CGTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG	5065	0.22445003095327676	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCC	4336	0.19214517950906373	No Hit
ACCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	4228	0.18735927559140256	No Hit
GCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	3968	0.1758376550488849	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGT	3737	0.16560113833610962	No Hit
ACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT	3586	0.15890973563641664	No Hit
TCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	3475	0.15399088994326487	No Hit
AGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	3184	0.14109553772067782	No Hit
TGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCTTG	3076	0.1363096338030166	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTC	3041	0.1347586464222931	No Hit
TGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	2664	0.11805229663564248	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0467392262877295	0.0
2	0.0	0.0	0.0	3.0225642075540176	0.0
3	0.0	0.0	0.0	4.835624141694662	0.0
4	0.0	0.0	0.0	6.011981599085715	0.0
5	0.0	0.0	0.0	10.727204806111068	0.0
6	0.0	0.0	0.0	12.175694077931356	0.0
7	4.431392516352946E-5	0.0	0.0	16.355915266457416	0.0
8	4.431392516352946E-5	0.0	0.0	21.166014587257884	0.0
9	8.862785032705892E-5	0.0	0.0	24.930216646348732	0.0
10	8.862785032705892E-5	0.0	0.0	27.2909966955106	0.0
11	8.862785032705892E-5	0.0	0.0	29.857925124533207	0.0
12	8.862785032705892E-5	0.0	0.0	31.685608653977816	0.0
13	8.862785032705892E-5	0.0	0.0	32.496686426245894	0.0
14	8.862785032705892E-5	0.0	0.0	33.05566227825866	0.0
15	8.862785032705892E-5	0.0	0.0	34.52590968733424	0.0
16	8.862785032705892E-5	0.0	0.0	36.33391783400624	0.0
17	8.862785032705892E-5	0.0	0.0	38.50233113403323	0.0
18	8.862785032705892E-5	0.0	0.0	39.78056630537524	0.0
19	8.862785032705892E-5	0.0	0.0	41.09629105740559	0.0
20	1.7725570065411784E-4	0.0	0.0	42.43621121257523	0.0
21	2.2156962581764732E-4	0.0	0.0	43.75796265842782	0.0
22	4.4313925163529463E-4	0.0	0.0	44.99884119085697	0.0
23	4.4313925163529463E-4	0.0	0.0	45.981591109208566	0.0
24	7.090228026164713E-4	0.0	0.0	46.768872303663834	0.0
25	7.090228026164713E-4	0.0	0.0	47.55123465242595	0.0
26	7.533367277800008E-4	0.0	0.0	48.32021419578867	0.0
27	8.419645781070598E-4	0.0	0.0	49.061275966298375	0.0
28	8.419645781070598E-4	0.0	0.0	49.83619357563301	0.0
29	8.419645781070598E-4	0.0	0.0	50.686311915970165	0.0
30	8.419645781070598E-4	0.0	0.0	51.44576396542273	0.0
31	8.419645781070598E-4	0.0	0.0	52.13533295489241	0.0
32	8.862785032705893E-4	0.0	0.0	52.78400019143616	0.0
33	8.862785032705893E-4	0.0	0.0	53.42061404033542	0.0
34	9.305924284341187E-4	0.0	0.0	54.10517555626162	0.0
35	9.305924284341187E-4	0.0	0.0	54.73527525816185	0.0
36	9.305924284341187E-4	0.0	0.0	55.40667553831448	0.0
37	9.305924284341187E-4	0.0	0.0	56.048429802532716	0.0
38	9.305924284341187E-4	0.0	0.0	56.724039905575886	0.0
39	9.305924284341187E-4	0.0	0.0	57.34864468075584	0.0
40	9.305924284341187E-4	0.0	0.0	57.95645447829881	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGGTA	20	6.312753E-4	46.0	3
TCCGTAC	20	6.312753E-4	46.0	46
CGTTTTT	30680	0.0	45.67014	1
ATTGCGG	370	0.0	44.756756	1
TGAACGG	160	0.0	44.5625	1
TACGCGG	265	0.0	44.264153	1
CCCGCAC	125	0.0	44.16	32
ACCCGCA	120	0.0	44.083332	31
AGTACGG	115	0.0	44.0	1
TCTACGG	225	0.0	43.955555	1
GTAGCGG	370	0.0	43.513515	1
AATGCGG	245	0.0	43.183674	1
ACGTAGG	155	0.0	43.032257	1
GCGAAGG	360	0.0	42.805557	1
ACTGCGG	285	0.0	42.77193	1
CCGCACT	125	0.0	42.32	33
GCGATAC	120	0.0	42.166668	8
GCGTAGG	415	0.0	42.12048	1
CTAGCGG	405	0.0	42.024693	1
TAAGCGG	460	0.0	42.0	1
>>END_MODULE