FastQCFastQC Report
Thu 26 May 2016
SRR1043323_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043323_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2242950
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1033874.609420629082236No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT219330.9778639737845249No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC196260.8750083595265165No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC121970.5437927729106757No Hit
CGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG106830.47629238279943825No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCC99260.44254218774381954No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC80590.35930359571100556No Hit
TCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC67660.3016563008537863No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT61610.27468289529414386No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCT56330.25114246862391043No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTC51200.22827080407499054No Hit
CGTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT50810.22653202255957555No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGC44090.19657147952473306No Hit
GCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG42700.19037428386722843No Hit
ACCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG41150.18346374194698947No Hit
TGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCTT37290.16625426335852336Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCG35290.15733743507434406No Hit
TCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG34260.15274526850799172No Hit
ACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC34030.15171983325531108No Hit
AGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG32600.14534430103212287No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGT30050.13397534496979424No Hit
TGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG27110.12086760739205066No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACG253.891997E-545.016
TCCGCTA207.034725E-445.041
ACGTTGC207.034725E-445.019
TTGTACG207.034725E-445.028
ATATCCG207.034725E-445.031
ACCTACG207.034725E-445.010
GCTTCGC207.034725E-445.030
TTCGACG600.044.99999624
CGTTTTT286050.044.6145741
CTAGCGG3800.044.4078941
CTAACGG1450.043.448281
CCAACGG1350.043.3333321
TTACCGG1300.043.2692341
TTTACGG2100.042.8571471
TATACGG2050.042.804881
TACGCGG2700.042.51
ACGGCCA750.042.06
ACCGAAC700.041.78571310
TAAGCGG3450.041.7391321
AACGCGG1350.041.6666641
Produced by FastQC (version 0.11.5)