##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043323_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2242950
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21553266902963	33.0	31.0	34.0	31.0	34.0
2	32.514806839207296	34.0	31.0	34.0	31.0	34.0
3	32.580737421699105	34.0	31.0	34.0	31.0	34.0
4	36.14823379923761	37.0	35.0	37.0	35.0	37.0
5	36.10192113065383	37.0	35.0	37.0	35.0	37.0
6	36.33597717291959	37.0	37.0	37.0	35.0	37.0
7	36.27849171849573	37.0	37.0	37.0	35.0	37.0
8	36.17686484317528	37.0	37.0	37.0	35.0	37.0
9	37.76210882988921	39.0	38.0	39.0	35.0	39.0
10	37.55003276934394	39.0	37.0	39.0	35.0	39.0
11	37.47706903854299	39.0	37.0	39.0	35.0	39.0
12	37.55069930225819	39.0	37.0	39.0	35.0	39.0
13	37.55241311665441	39.0	37.0	39.0	35.0	39.0
14	38.91507612742148	40.0	38.0	41.0	35.0	41.0
15	38.9974992755077	41.0	38.0	41.0	35.0	41.0
16	39.05499632180833	41.0	38.0	41.0	35.0	41.0
17	38.992408658240265	41.0	38.0	41.0	35.0	41.0
18	38.78558238034731	40.0	38.0	41.0	35.0	41.0
19	38.5682899752558	40.0	37.0	41.0	35.0	41.0
20	38.24689716667781	40.0	35.0	41.0	35.0	41.0
21	38.21939499320092	40.0	35.0	41.0	35.0	41.0
22	38.20625381751711	40.0	35.0	41.0	35.0	41.0
23	38.17465391560222	40.0	35.0	41.0	35.0	41.0
24	38.11281259056154	40.0	35.0	41.0	34.0	41.0
25	38.10269154461758	40.0	35.0	41.0	34.0	41.0
26	38.09870839742304	40.0	35.0	41.0	34.0	41.0
27	38.08557212599479	40.0	35.0	41.0	35.0	41.0
28	38.0145433469315	40.0	35.0	41.0	34.0	41.0
29	38.00014891103235	40.0	35.0	41.0	34.0	41.0
30	37.84725428565059	40.0	35.0	41.0	34.0	41.0
31	37.6625729508014	40.0	35.0	41.0	34.0	41.0
32	37.450199068191445	40.0	35.0	41.0	33.0	41.0
33	37.04529525847656	40.0	35.0	41.0	32.0	41.0
34	36.777047192313695	40.0	35.0	41.0	31.0	41.0
35	36.63250852671705	40.0	35.0	41.0	31.0	41.0
36	36.53153079649569	40.0	35.0	41.0	30.0	41.0
37	36.42847901201542	40.0	35.0	41.0	30.0	41.0
38	36.38145522637598	40.0	35.0	41.0	30.0	41.0
39	36.35811810339062	40.0	35.0	41.0	30.0	41.0
40	36.31484651909316	40.0	35.0	41.0	30.0	41.0
41	36.23878686551194	40.0	35.0	41.0	30.0	41.0
42	36.140937604494084	39.0	35.0	41.0	29.0	41.0
43	36.02935330702869	39.0	35.0	41.0	29.0	41.0
44	35.9505459328117	39.0	35.0	41.0	29.0	41.0
45	35.90230098753873	39.0	35.0	41.0	28.0	41.0
46	35.82026705900711	39.0	35.0	41.0	27.0	41.0
47	35.69499810517399	39.0	35.0	41.0	27.0	41.0
48	35.67494950845984	39.0	35.0	41.0	27.0	41.0
49	35.643202478878266	39.0	35.0	41.0	27.0	41.0
50	35.568934216099336	39.0	35.0	40.0	26.0	41.0
51	35.12317483671058	38.0	35.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	1.0
12	0.0
13	5.0
14	10.0
15	13.0
16	42.0
17	106.0
18	263.0
19	577.0
20	1078.0
21	2027.0
22	3311.0
23	5453.0
24	10554.0
25	20616.0
26	32546.0
27	37214.0
28	35562.0
29	33287.0
30	33792.0
31	38386.0
32	46506.0
33	59636.0
34	102378.0
35	154579.0
36	193555.0
37	181616.0
38	313968.0
39	935587.0
40	280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.34369914621369	18.326846340756592	25.881227847254735	29.448226665774985
2	34.65792817494817	18.916783700037897	25.404712543748186	21.020575581265742
3	21.23814619139972	20.882988920840855	38.355424775407386	19.523440112352038
4	23.02494482712499	23.002206915000333	35.45571680153369	18.51713145634098
5	20.74981609041664	28.026393811721174	31.750061303194453	19.473728794667736
6	72.14516596446644	1.3361867183842708	22.13937002608172	4.3792772910675675
7	72.42533270915536	1.1786263626028222	18.717804676876433	7.678236251365389
8	60.6703671504046	3.615907621658976	26.465458436434158	9.248266791502264
9	30.149312289618585	25.589647562362067	28.23732138478343	16.023718763235916
10	19.665574355201855	18.865066095989658	47.61158295994115	13.85777658886734
11	18.454535321786043	16.245792371653405	43.531420673666375	21.768251632894177
12	18.242270224481153	17.004926547627008	46.513163467754524	18.23963976013732
13	14.472547314920083	21.347332753739494	44.2733007869101	19.906819144430326
14	12.943088343476225	22.645310862926056	44.70946744243073	19.70213335116699
15	13.5414966896275	21.623843598831897	45.30631534363227	19.528344367908335
16	14.129561514969124	24.87126329164716	40.626050513832226	20.373124679551484
17	13.759646893599948	21.953810829487953	42.222519449831694	22.064022827080407
18	15.103100827035822	27.52312802336209	41.383178403441896	15.990592746160189
19	15.489511580730733	23.38139503778506	38.419759691477736	22.709333690006464
20	13.656256269644887	28.646247129895897	38.036068570409505	19.66142803004971
21	14.34017699904144	23.356383334447937	39.21821708018458	23.085222586326044
22	13.245948416148376	25.435341848904347	35.23038855079248	26.088321184154793
23	12.261753494282084	22.87821841770882	41.631378318732025	23.22864976927707
24	17.12476871976638	21.560266613165695	38.46501259501996	22.849952072047973
25	12.763637174257116	23.76825163289418	40.0200628636394	23.4480483292093
26	14.129695267393389	22.082257740921555	39.579259457411	24.20878753427406
27	18.38137274571435	24.166833857196995	38.727345683140506	18.724447713948148
28	15.436857709712656	25.10359125259145	35.066363494505005	24.393187543190887
29	17.33315499676765	22.745179339708866	37.6765420539914	22.24512360953209
30	18.49470563320627	25.347644842729437	33.04300140440046	23.114648119663837
31	16.789941817695446	25.040460108339463	34.95468022024566	23.214917853719435
32	17.7403419603647	24.15167524911389	35.22111504937694	22.886867741144474
33	12.886733988720211	25.628837022671036	40.5549388082659	20.929490180342853
34	15.823402215831827	25.601952785394232	34.44263135602666	24.132013642747275
35	18.059921086069686	23.312200450299827	35.82197552330636	22.805902940324128
36	17.279743195345414	22.320381640250563	38.67522682181948	21.724648342584544
37	15.301946097773023	23.06903854299026	37.39160480617045	24.237410553066276
38	15.074210303395082	20.478566174011902	35.90949419291558	28.537729329677436
39	19.403018346374196	18.096970507590452	40.205978733364546	22.294032412670813
40	14.486591319467665	17.787868655119375	42.535277201899284	25.190262823513677
41	17.454958871129538	22.400410174101072	34.02648298000401	26.118147974765378
42	18.263848948928864	19.614168840143563	37.78425733966428	24.33772487126329
43	21.756213914710536	21.385942620209992	36.7779040995118	20.079939365567668
44	17.476715932142938	21.293118437771685	37.24840054392652	23.981765086158855
45	18.018101161416883	20.33357854611115	36.13780958113199	25.51051071133998
46	20.902695111348894	18.432020330368488	35.997859961211795	24.667424597070823
47	16.201074477808245	17.96562562696449	39.261998707059895	26.571301188167368
48	17.413896876880894	16.28186094206291	35.65268953833122	30.651552642724983
49	19.074522392385028	15.555540694175082	40.12242805234178	25.247508861098105
50	18.35756481419559	14.25947970306962	40.13277157315143	27.25018390958336
51	15.948683653224549	14.856149267705476	38.43126239996433	30.763904679105643
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	352.0
1	2046.0
2	3740.0
3	65445.5
4	127151.0
5	73140.0
6	19129.0
7	18035.0
8	16941.0
9	17222.5
10	17504.0
11	17483.5
12	17463.0
13	16799.5
14	16136.0
15	15417.0
16	14698.0
17	13842.0
18	12986.0
19	12520.0
20	12054.0
21	11387.5
22	10721.0
23	11046.0
24	11371.0
25	11966.0
26	14007.0
27	15453.0
28	17182.0
29	18911.0
30	21046.0
31	23181.0
32	26107.0
33	29033.0
34	33518.5
35	38004.0
36	43174.0
37	48344.0
38	56485.0
39	64626.0
40	78026.0
41	91426.0
42	114838.5
43	138251.0
44	192523.5
45	246796.0
46	307862.0
47	368928.0
48	354976.5
49	341025.0
50	271275.5
51	201526.0
52	156503.0
53	111480.0
54	87619.0
55	63758.0
56	52291.5
57	40825.0
58	35472.0
59	30119.0
60	26343.0
61	22567.0
62	20901.5
63	19236.0
64	16876.0
65	14516.0
66	11833.5
67	9151.0
68	6890.0
69	4629.0
70	3704.5
71	2780.0
72	2494.5
73	2209.0
74	1886.5
75	1223.5
76	883.0
77	663.5
78	444.0
79	351.5
80	259.0
81	191.5
82	124.0
83	91.5
84	59.0
85	35.5
86	12.0
87	10.0
88	8.0
89	6.5
90	5.0
91	6.5
92	8.0
93	4.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2242950.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.7416010055545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.15958380732121	23.298787007406276
2	13.081395113495287	8.56611638804146
3	5.253847534993642	5.160581392043335
4	2.673140628473712	3.5009161555689388
5	1.5436638861955783	2.5271013524249653
6	0.9858780236818796	1.9367534934922028
7	0.7355347738576726	1.6857810263951074
8	0.5476383026430465	1.4344443840398182
9	0.44189947091074455	1.302164654501271
>10	3.199133312854178	19.704553528089566
>50	0.19129049786487945	4.354559803851846
>100	0.16826829389741507	11.094160548077133
>500	0.012530133799912364	2.7822162760586906
>1k	0.004543894674693494	2.97828288414093
>5k	9.638564461471049E-4	2.107456808786472
>10k+	6.88468890105075E-4	7.56612429708207
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	103387	4.609420629082236	No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT	21933	0.9778639737845249	No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	19626	0.8750083595265165	No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	12197	0.5437927729106757	No Hit
CGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG	10683	0.47629238279943825	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCC	9926	0.44254218774381954	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC	8059	0.35930359571100556	No Hit
TCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	6766	0.3016563008537863	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT	6161	0.27468289529414386	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCT	5633	0.25114246862391043	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTC	5120	0.22827080407499054	No Hit
CGTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT	5081	0.22653202255957555	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGC	4409	0.19657147952473306	No Hit
GCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG	4270	0.19037428386722843	No Hit
ACCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG	4115	0.18346374194698947	No Hit
TGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCTT	3729	0.16625426335852336	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCG	3529	0.15733743507434406	No Hit
TCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG	3426	0.15274526850799172	No Hit
ACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	3403	0.15171983325531108	No Hit
AGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG	3260	0.14534430103212287	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGT	3005	0.13397534496979424	No Hit
TGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG	2711	0.12086760739205066	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0294032412670813	0.0
2	0.0	0.0	0.0	2.996723065605564	0.0
3	0.0	0.0	0.0	4.820392786285918	0.0
4	0.0	0.0	0.0	6.013152321719165	0.0
5	0.0	0.0	0.0	10.839207293965536	0.0
6	0.0	0.0	0.0	12.304688022470406	0.0
7	0.0	0.0	0.0	16.58668271695758	0.0
8	0.0	0.0	0.0	21.545687598921063	0.0
9	0.0	0.0	0.0	25.446755389108095	0.0
10	0.0	0.0	0.0	27.874807730890122	0.0
11	0.0	0.0	0.0	30.493769366236428	0.0
12	0.0	0.0	0.0	32.385741991573596	0.0
13	0.0	0.0	0.0	33.233063599277735	0.0
14	0.0	0.0	0.0	33.80382977774806	0.0
15	0.0	0.0	0.0	35.318620566664436	0.0
16	4.4584141420896585E-5	0.0	0.0	37.19133284290778	0.0
17	4.4584141420896585E-5	0.0	0.0	39.427450455872844	0.0
18	4.4584141420896585E-5	0.0	0.0	40.76247798658017	0.0
19	4.4584141420896585E-5	0.0	0.0	42.12826857486792	0.0
20	4.4584141420896585E-5	0.0	0.0	43.498963418711966	0.0
21	4.4584141420896585E-5	0.0	0.0	44.84179317416795	0.0
22	2.2292070710448294E-4	0.0	0.0	46.11132660112798	0.0
23	2.675048485253795E-4	0.0	0.0	47.09752780935821	0.0
24	4.904255556298625E-4	0.0	0.0	47.90913751978421	0.0
25	4.904255556298625E-4	0.0	0.0	48.7121870750574	0.0
26	5.35009697050759E-4	0.0	0.0	49.49432666800419	0.0
27	5.795938384716556E-4	0.0	0.0	50.25319333912927	0.0
28	5.795938384716556E-4	0.0	0.0	51.03025033995408	0.0
29	5.795938384716556E-4	0.0	0.0	51.8935330702869	0.0
30	5.795938384716556E-4	0.0	0.0	52.65132972201788	0.0
31	5.795938384716556E-4	0.0	0.0	53.341982656768984	0.0
32	5.795938384716556E-4	0.0	0.0	53.99634410040348	0.0
33	5.795938384716556E-4	0.0	0.0	54.65017053434094	0.0
34	5.795938384716556E-4	0.0	0.0	55.33114871040371	0.0
35	5.795938384716556E-4	0.0	0.0	55.96486769656033	0.0
36	5.795938384716556E-4	0.0	0.0	56.65324684009898	0.0
37	5.795938384716556E-4	0.0	0.0	57.285672886154394	0.0
38	5.795938384716556E-4	0.0	0.0	57.957511313225886	0.0
39	5.795938384716556E-4	0.0	0.0	58.588421498473	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACG	25	3.891997E-5	45.0	16
TCCGCTA	20	7.034725E-4	45.0	41
ACGTTGC	20	7.034725E-4	45.0	19
TTGTACG	20	7.034725E-4	45.0	28
ATATCCG	20	7.034725E-4	45.0	31
ACCTACG	20	7.034725E-4	45.0	10
GCTTCGC	20	7.034725E-4	45.0	30
TTCGACG	60	0.0	44.999996	24
CGTTTTT	28605	0.0	44.614574	1
CTAGCGG	380	0.0	44.407894	1
CTAACGG	145	0.0	43.44828	1
CCAACGG	135	0.0	43.333332	1
TTACCGG	130	0.0	43.269234	1
TTTACGG	210	0.0	42.857147	1
TATACGG	205	0.0	42.80488	1
TACGCGG	270	0.0	42.5	1
ACGGCCA	75	0.0	42.0	6
ACCGAAC	70	0.0	41.785713	10
TAAGCGG	345	0.0	41.739132	1
AACGCGG	135	0.0	41.666664	1
>>END_MODULE