FastQCFastQC Report
Thu 26 May 2016
SRR1043322_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043322_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1139151
Sequences flagged as poor quality0
Sequence length52
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT825907.250136285707513No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT78530.6893730506315668Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT70890.6223055591400964Illumina Single End Adapter 2 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC50180.44050349778036446Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT45610.40038590143010017Illumina Single End Adapter 2 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT36750.32260867962192896No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTC31370.2753805246187731No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCG26550.23306831140033232No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTT25630.22499212132544325No Hit
TCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT24160.2120877741405661Illumina Single End Adapter 2 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG19280.169248852873763No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCT17730.1556422282910694No Hit
ACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC17050.14967287040962965Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC16430.1442302205765522Illumina Single End Adapter 2 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC14880.1306235959938586Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC13290.11666583271225676Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT12400.10885299666154881Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC800.046.00000424
CGAACTC351.01956175E-746.00000430
GTCGAGC206.3114293E-446.0000041
CGACGGT800.046.00000427
CTATCGG351.01956175E-746.0000041
GCGAACT351.01956175E-746.00000429
CGTTGAC206.3114293E-446.00000430
TAACGTG206.3114293E-446.00000444
ACTACGG800.046.0000041
TCGTATC351.01956175E-746.0000046
TACTCGA206.3114293E-446.00000419
GCGTGAC206.3114293E-446.0000048
GCTACGG1450.046.0000041
TATCGGT351.01956175E-746.0000049
TACCGTA206.3114293E-446.00000414
TAGTAAC206.3114293E-446.00000410
TCGTTAG253.4171368E-546.014
CGAGTTG301.8613882E-646.034
CCTACGG501.6370905E-1146.01
ACGATGG253.4171368E-546.01
Produced by FastQC (version 0.11.5)