##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043322_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1139151
Sequences flagged as poor quality	0
Sequence length	52
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26277025609423	33.0	31.0	34.0	31.0	34.0
2	32.59839125805095	34.0	31.0	34.0	31.0	34.0
3	32.658387693993156	34.0	31.0	34.0	31.0	34.0
4	36.18661705076851	37.0	35.0	37.0	35.0	37.0
5	36.0841345879519	37.0	35.0	37.0	35.0	37.0
6	36.322716654771845	37.0	37.0	37.0	35.0	37.0
7	36.25639884440254	37.0	37.0	37.0	35.0	37.0
8	36.23220626589451	37.0	37.0	37.0	35.0	37.0
9	37.76561052924502	39.0	38.0	39.0	35.0	39.0
10	37.593593825577116	39.0	37.0	39.0	35.0	39.0
11	37.29606347183122	39.0	37.0	39.0	34.0	39.0
12	37.31761285378321	39.0	37.0	39.0	34.0	39.0
13	37.3059743616079	39.0	37.0	39.0	34.0	39.0
14	38.72677019991204	40.0	38.0	41.0	35.0	41.0
15	38.82382493629027	41.0	38.0	41.0	35.0	41.0
16	38.841676827742766	41.0	38.0	41.0	35.0	41.0
17	38.81462598022562	41.0	38.0	41.0	35.0	41.0
18	38.49379581811367	40.0	38.0	41.0	35.0	41.0
19	38.16115598371068	40.0	37.0	41.0	34.0	41.0
20	37.80664284190595	40.0	35.0	41.0	34.0	41.0
21	37.73565752038141	39.0	35.0	41.0	34.0	41.0
22	37.69331458252681	39.0	35.0	41.0	34.0	41.0
23	37.689691709000826	39.0	35.0	41.0	34.0	41.0
24	37.59299425624873	39.0	35.0	41.0	34.0	41.0
25	37.511312372108705	39.0	35.0	41.0	34.0	41.0
26	37.46105652367421	39.0	35.0	41.0	34.0	41.0
27	37.46956900358249	39.0	35.0	41.0	34.0	41.0
28	37.45772158388133	39.0	35.0	41.0	33.0	41.0
29	37.39534618325402	39.0	35.0	41.0	33.0	41.0
30	37.336784148896854	39.0	35.0	41.0	33.0	41.0
31	37.1530912056435	39.0	35.0	41.0	33.0	41.0
32	36.90320071702522	39.0	35.0	41.0	33.0	41.0
33	36.590739945801744	39.0	35.0	41.0	31.0	41.0
34	36.32454784308665	39.0	35.0	41.0	31.0	41.0
35	36.1577560832585	39.0	35.0	41.0	30.0	41.0
36	36.08698846772728	39.0	35.0	41.0	30.0	41.0
37	36.048367600081114	39.0	35.0	41.0	30.0	41.0
38	35.96958787728756	39.0	35.0	41.0	29.0	41.0
39	35.9421560442821	39.0	35.0	41.0	29.0	41.0
40	35.89277365336115	39.0	35.0	41.0	29.0	41.0
41	35.8616855886533	39.0	35.0	41.0	29.0	41.0
42	35.78398912874588	39.0	35.0	41.0	28.0	41.0
43	35.6789275521858	39.0	35.0	41.0	28.0	41.0
44	35.521493638683545	39.0	35.0	41.0	27.0	41.0
45	35.44911078513735	38.0	35.0	41.0	26.0	41.0
46	35.423409188070764	38.0	35.0	41.0	25.0	41.0
47	35.34732621048482	38.0	35.0	41.0	25.0	41.0
48	35.296121409716534	38.0	35.0	41.0	25.0	41.0
49	35.24142804597459	38.0	35.0	41.0	25.0	41.0
50	35.109233104303115	38.0	35.0	40.0	24.0	41.0
51	35.01795459952192	38.0	35.0	40.0	24.0	41.0
52	34.71457164151197	38.0	35.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	2.0
14	7.0
15	15.0
16	37.0
17	106.0
18	238.0
19	453.0
20	883.0
21	1406.0
22	2367.0
23	3962.0
24	7565.0
25	14286.0
26	19398.0
27	20602.0
28	18480.0
29	17239.0
30	17976.0
31	20840.0
32	25115.0
33	33345.0
34	59320.0
35	102334.0
36	119749.0
37	83601.0
38	137391.0
39	430815.0
40	1618.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.813522526864308	18.186351063204086	25.43350266997088	30.566623739960725
2	35.59931914206282	19.1263493601814	25.07736024460322	20.19697125315257
3	20.244287192830452	20.558117404979672	39.699477944539396	19.49811745765048
4	21.704585256915017	23.723896129661476	37.56490579387632	17.00661281954719
5	19.731361338400262	27.986544365057835	33.57272214131402	18.709372155227886
6	70.69607102131324	1.5321059280112996	23.71485430816459	4.05696874251087
7	71.45444282628027	1.346090202264669	20.56496460960838	6.634502361846674
8	60.769028864478905	3.2543534614814016	27.53357544346623	8.44304223057347
9	33.04136150519115	18.27123884366515	31.099388930879222	17.588010720264478
10	25.392419442198623	20.15044537554723	42.341182161100676	12.115953021153473
11	22.206713596353776	16.838944090818515	42.199322126741755	18.755020186085954
12	18.090139059703237	19.678163825515668	44.20414852815825	18.027548586622842
13	13.913432020864661	22.273517733821066	43.388716684618636	20.42433356069564
14	12.268610570503823	22.1970572821338	45.030729025388204	20.503603121974173
15	13.756999730501049	18.799702585522024	47.41022041853977	20.033077265437154
16	14.219712750987359	24.55469029127833	39.92315329574394	21.302443661990377
17	15.368901927839243	21.670524803120923	42.606379663451115	20.354193605588726
18	17.05155857300744	27.53928144732349	39.56183157456737	15.847328405101694
19	16.96421282165402	23.261534247874074	37.81816458046387	21.95608835000803
20	13.988751271780476	28.702516172131702	36.83243046795376	20.47630208813406
21	14.557683748686523	23.13644108638802	38.8870307799405	23.418844384984958
22	13.085885892212708	26.159130791264722	35.17558251715532	25.579400799367246
23	12.771265617990943	22.57988624861849	44.16280194636181	20.486046187028762
24	16.868703095550984	21.671666003892373	40.10100504674095	21.358625853815695
25	13.955656449408375	25.338958575289844	39.49134048076155	21.214044494540232
26	14.859926383771773	23.194817895081513	37.966871819451505	23.97838390169521
27	18.38456885873778	23.2648700655137	38.65062665090054	19.699934424847978
28	14.352443179174665	22.799172366086673	35.96617129774718	26.88221315699148
29	15.62479425466861	26.923296384763738	34.62771836218377	22.824190998383884
30	17.749095598388624	24.01147872406731	33.10052837595718	25.138897301586884
31	16.58708985902659	25.565618605435102	32.3582211664652	25.489070369073108
32	14.524150002940786	30.786348780802548	31.158994725018896	23.530506491237773
33	12.822356298682088	25.169534153066625	35.56622431969071	26.44188522856057
34	12.4502370625141	29.54261550926962	32.93856565108577	25.06858177713051
35	18.262460376192447	26.58163843072604	33.83142357773465	21.32447761534687
36	14.233933868293141	31.996372737240282	33.77603144798187	19.993661946484707
37	16.95499543080768	27.97381558722241	36.8464760159101	18.224712966059812
38	16.933400400824823	29.725646556075535	32.46417726886076	20.876775774238883
39	20.828845341837912	27.20710423815631	34.49560242671954	17.46844799328623
40	15.476701508404068	24.749221130473483	37.960551322871154	21.813526038251293
41	18.137454999381116	25.93176848372165	31.931236508592804	23.99954000830443
42	17.75164135395571	22.331894542514558	36.37085864823891	23.545605455290826
43	20.03887105396914	24.253764426313985	34.88940447754512	20.817960042171755
44	14.890036527203154	26.64168314823935	35.72748476716432	22.74079555739318
45	17.78710636254544	23.77156320803827	35.38828478401898	23.05304564539732
46	20.766167084082795	22.83533965207422	33.40970600034587	22.988787263497112
47	16.270801675985012	21.218433728276583	37.51916997834352	24.991594617394885
48	16.451462536573292	20.063801901591624	34.30352955841675	29.181206003418335
49	17.94424093030687	19.886915782016608	38.69557240436079	23.473270883315735
50	17.06885215392867	19.419199035070854	39.25397072029959	24.25797809070088
51	15.156638584349222	19.147066543416983	38.04552688800695	27.65076798422685
52	14.282917716790838	19.067972551487905	43.863631774892006	22.785477956829254
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	407.0
1	1550.0
2	2693.0
3	47746.0
4	92799.0
5	51472.5
6	10146.0
7	9699.5
8	9253.0
9	9701.5
10	10150.0
11	10379.5
12	10609.0
13	10348.5
14	9443.0
15	8798.0
16	7987.5
17	7177.0
18	6618.5
19	6060.0
20	5568.5
21	5077.0
22	4942.5
23	4808.0
24	4781.0
25	4754.0
26	5215.0
27	5676.0
28	6269.5
29	6863.0
30	6895.0
31	6927.0
32	8516.5
33	10106.0
34	12065.0
35	14024.0
36	17093.5
37	20163.0
38	22823.5
39	28768.5
40	32053.0
41	43143.0
42	54233.0
43	77539.0
44	100845.0
45	128811.5
46	156778.0
47	159577.5
48	162377.0
49	143608.0
50	124839.0
51	102327.0
52	79815.0
53	65418.5
54	51022.0
55	41833.0
56	32644.0
57	28137.0
58	23630.0
59	20344.5
60	17059.0
61	14030.0
62	11001.0
63	9047.5
64	5656.5
65	4219.0
66	3468.0
67	2717.0
68	2294.0
69	1871.0
70	1576.5
71	1282.0
72	1391.0
73	1500.0
74	1209.0
75	918.0
76	702.5
77	487.0
78	453.0
79	419.0
80	279.0
81	139.0
82	104.5
83	70.0
84	51.5
85	33.0
86	28.5
87	24.0
88	19.5
89	8.5
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1139151.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.78612464409995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.0859468911143	26.119491075745714
2	11.614535321094236	8.08049346725771
3	4.365979680085302	4.5562654003516485
4	2.101639064380244	2.9243151380176298
5	1.2326915106306746	2.1440280268261254
6	0.7721159379371139	1.6115352754065948
7	0.5511152428936108	1.3419814472792393
8	0.4322601773784367	1.2029325127173642
9	0.32170213692967126	1.0071693570157967
>10	3.1018694185157467	22.643791193482173
>50	0.26863091515805887	6.2827937630617505
>100	0.1403841496780721	8.694776268402023
>500	0.006070665929768986	1.4387172799286814
>1k	0.00404711061984599	2.9283805145438473
>5k	7.588332412211233E-4	1.7562715985185298
>10k+	2.529444137403744E-4	7.267057681445159
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82590	7.250136285707513	No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	7853	0.6893730506315668	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT	7089	0.6223055591400964	Illumina Single End Adapter 2 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	5018	0.44050349778036446	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	4561	0.40038590143010017	Illumina Single End Adapter 2 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT	3675	0.32260867962192896	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTC	3137	0.2753805246187731	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCG	2655	0.23306831140033232	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTT	2563	0.22499212132544325	No Hit
TCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	2416	0.2120877741405661	Illumina Single End Adapter 2 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG	1928	0.169248852873763	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCT	1773	0.1556422282910694	No Hit
ACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1705	0.14967287040962965	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1643	0.1442302205765522	Illumina Single End Adapter 2 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1488	0.1306235959938586	Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1329	0.11666583271225676	Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	1240	0.10885299666154881	Illumina Single End Adapter 2 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6396869247360534	0.0
2	0.0	0.0	0.0	2.0882218424071963	0.0
3	0.0	0.0	0.0	3.5672180422086273	0.0
4	0.0	0.0	0.0	4.460163753532236	0.0
5	0.0	0.0	0.0	8.85808817268299	0.0
6	0.0	0.0	0.0	10.402220601131896	0.0
7	0.0	0.0	0.0	14.253685420106729	0.0
8	0.0	0.0	0.0	18.68689927849776	0.0
9	0.0	0.0	0.0	22.064239069271764	0.0
10	8.77846747270555E-5	0.0	0.0	24.09461081103383	0.0
11	8.77846747270555E-5	0.0	0.0	25.90552086597826	0.0
12	8.77846747270555E-5	0.0	0.0	27.22562680452372	0.0
13	8.77846747270555E-5	0.0	0.0	27.8290586585975	0.0
14	8.77846747270555E-5	0.0	0.0	28.267191970160233	0.0
15	8.77846747270555E-5	0.0	0.0	29.45351406442166	0.0
16	8.77846747270555E-5	0.0	0.0	30.90046885794772	0.0
17	8.77846747270555E-5	0.0	0.0	32.474009152430185	0.0
18	8.77846747270555E-5	0.0	0.0	33.37222194423742	0.0
19	1.75569349454111E-4	0.0	0.0	34.32292997153143	0.0
20	1.75569349454111E-4	0.0	0.0	35.43279161410559	0.0
21	1.75569349454111E-4	0.0	0.0	36.528081000675066	0.0
22	3.51138698908222E-4	0.0	0.0	37.63372897886233	0.0
23	4.389233736352775E-4	0.0	0.0	38.51851071543632	0.0
24	7.02277397816444E-4	0.0	0.0	39.260642355578845	0.0
25	7.02277397816444E-4	0.0	0.0	39.95975950510512	0.0
26	7.900620725434995E-4	0.0	0.0	40.6816128853857	0.0
27	7.900620725434995E-4	0.0	0.0	41.37686750922398	0.0
28	7.900620725434995E-4	0.0	0.0	42.064572651035725	0.0
29	7.900620725434995E-4	0.0	0.0	42.866573439342105	0.0
30	7.900620725434995E-4	0.0	0.0	43.568411913784914	0.0
31	7.900620725434995E-4	0.0	0.0	44.18167565142812	0.0
32	7.900620725434995E-4	0.0	0.0	44.80345450251986	0.0
33	7.900620725434995E-4	0.0	0.0	45.404516170376006	0.0
34	7.900620725434995E-4	0.0	0.0	46.11460640424316	0.0
35	7.900620725434995E-4	0.0	0.0	46.69793556780444	0.0
36	7.900620725434995E-4	0.0	0.0	47.270555001049026	0.0
37	7.900620725434995E-4	0.0	0.0	47.84335000364307	0.0
38	7.900620725434995E-4	0.0	0.0	48.40736653876439	0.0
39	7.900620725434995E-4	0.0	0.0	48.9625168217383	0.0
40	7.900620725434995E-4	0.0	0.0	49.5115221774813	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	80	0.0	46.000004	24
CGAACTC	35	1.01956175E-7	46.000004	30
GTCGAGC	20	6.3114293E-4	46.000004	1
CGACGGT	80	0.0	46.000004	27
CTATCGG	35	1.01956175E-7	46.000004	1
GCGAACT	35	1.01956175E-7	46.000004	29
CGTTGAC	20	6.3114293E-4	46.000004	30
TAACGTG	20	6.3114293E-4	46.000004	44
ACTACGG	80	0.0	46.000004	1
TCGTATC	35	1.01956175E-7	46.000004	6
TACTCGA	20	6.3114293E-4	46.000004	19
GCGTGAC	20	6.3114293E-4	46.000004	8
GCTACGG	145	0.0	46.000004	1
TATCGGT	35	1.01956175E-7	46.000004	9
TACCGTA	20	6.3114293E-4	46.000004	14
TAGTAAC	20	6.3114293E-4	46.000004	10
TCGTTAG	25	3.4171368E-5	46.0	14
CGAGTTG	30	1.8613882E-6	46.0	34
CCTACGG	50	1.6370905E-11	46.0	1
ACGATGG	25	3.4171368E-5	46.0	1
>>END_MODULE