SRR1043321_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043321_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1116165
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	76905	6.890110333149668	No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	7494	0.6714061093117953	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	6853	0.6139773241411439	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG	4809	0.43085027751273336	No Hit
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	4445	0.3982386116747972	Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTC	3446	0.3087357155976043	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTT	3049	0.2731674976369981	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCC	2439	0.21851607961188535	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCT	2426	0.21735137726053044	No Hit
TCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	2308	0.20677946360977095	Illumina Single End Adapter 2 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT	1993	0.17855782971155698	No Hit
GCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG	1715	0.15365111789027608	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTC	1690	0.15141130567613212	No Hit
ACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG	1607	0.14397512912517416	No Hit
TCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG	1392	0.12471274408353604	No Hit
AGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG	1317	0.11799330744110413	No Hit
ACTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1261	0.11297612808142164	Illumina Single End Adapter 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT	1223	0.10957161351592283	Illumina Single End Adapter 2 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	115	0.0	45.000004	5
CCTACGG	30	2.165445E-6	45.000004	1
AGGGTCG	30	2.165445E-6	45.000004	5
GTCACGG	110	0.0	45.000004	1
ACTACGG	60	0.0	45.000004	1
AGCCGTG	20	7.0331997E-4	45.0	24
GATACGA	20	7.0331997E-4	45.0	10
CAGCCGT	20	7.0331997E-4	45.0	23
CGGCCAC	35	1.2118835E-7	45.0	22
TGCGGTA	45	3.8562575E-10	45.0	21
TTATCGG	35	1.2118835E-7	45.0	1
GCACCGG	40	6.8139343E-9	45.0	1
ATGTACG	20	7.0331997E-4	45.0	11
CTAACGG	45	3.8562575E-10	45.0	1
CTACGGC	45	3.8562575E-10	45.0	2
ACGTGCG	45	3.8562575E-10	45.0	18
TCCACCG	35	1.2118835E-7	45.0	30
ACCGTTC	20	7.0331997E-4	45.0	9
TGAGGCG	20	7.0331997E-4	45.0	3
GACGAGC	20	7.0331997E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content