FastQCFastQC Report
Thu 26 May 2016
SRR1043321_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043321_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1116165
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT769056.890110333149668No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC74940.6714061093117953Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT68530.6139773241411439Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG48090.43085027751273336No Hit
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC44450.3982386116747972Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTC34460.3087357155976043No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTT30490.2731674976369981No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCC24390.21851607961188535No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCT24260.21735137726053044No Hit
TCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC23080.20677946360977095Illumina Single End Adapter 2 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT19930.17855782971155698No Hit
GCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG17150.15365111789027608No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTC16900.15141130567613212No Hit
ACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG16070.14397512912517416No Hit
TCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG13920.12471274408353604No Hit
AGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG13170.11799330744110413No Hit
ACTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC12610.11297612808142164Illumina Single End Adapter 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT12230.10957161351592283Illumina Single End Adapter 2 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT1150.045.0000045
CCTACGG302.165445E-645.0000041
AGGGTCG302.165445E-645.0000045
GTCACGG1100.045.0000041
ACTACGG600.045.0000041
AGCCGTG207.0331997E-445.024
GATACGA207.0331997E-445.010
CAGCCGT207.0331997E-445.023
CGGCCAC351.2118835E-745.022
TGCGGTA453.8562575E-1045.021
TTATCGG351.2118835E-745.01
GCACCGG406.8139343E-945.01
ATGTACG207.0331997E-445.011
CTAACGG453.8562575E-1045.01
CTACGGC453.8562575E-1045.02
ACGTGCG453.8562575E-1045.018
TCCACCG351.2118835E-745.030
ACCGTTC207.0331997E-445.09
TGAGGCG207.0331997E-445.03
GACGAGC207.0331997E-445.01