##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043321_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1116165
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21275617852199	33.0	31.0	34.0	31.0	34.0
2	32.52344321852056	34.0	31.0	34.0	31.0	34.0
3	32.56749584514834	34.0	31.0	34.0	31.0	34.0
4	36.145508056604534	37.0	35.0	37.0	35.0	37.0
5	36.09759757741911	37.0	35.0	37.0	35.0	37.0
6	36.3272697137072	37.0	37.0	37.0	35.0	37.0
7	36.27652004855913	37.0	37.0	37.0	35.0	37.0
8	36.18002714652403	37.0	37.0	37.0	35.0	37.0
9	37.72267272311889	39.0	38.0	39.0	35.0	39.0
10	37.54446609596251	39.0	37.0	39.0	35.0	39.0
11	37.29708869208405	39.0	37.0	39.0	34.0	39.0
12	37.32269064161661	39.0	37.0	39.0	34.0	39.0
13	37.31702570856459	39.0	37.0	39.0	34.0	39.0
14	38.74611549367701	40.0	38.0	41.0	35.0	41.0
15	38.85454032333929	41.0	38.0	41.0	35.0	41.0
16	38.89645706503967	41.0	38.0	41.0	35.0	41.0
17	38.85248686350136	41.0	38.0	41.0	35.0	41.0
18	38.562371154802385	40.0	38.0	41.0	35.0	41.0
19	38.256341132359466	40.0	37.0	41.0	35.0	41.0
20	37.93364959481797	40.0	35.0	41.0	34.0	41.0
21	37.86301935645715	40.0	35.0	41.0	34.0	41.0
22	37.8373305022107	40.0	35.0	41.0	34.0	41.0
23	37.80212871752832	40.0	35.0	41.0	34.0	41.0
24	37.71282202900109	39.0	35.0	41.0	34.0	41.0
25	37.69227936729784	39.0	35.0	41.0	34.0	41.0
26	37.66018375419405	39.0	35.0	41.0	34.0	41.0
27	37.6487302504558	39.0	35.0	41.0	34.0	41.0
28	37.55877132861181	39.0	35.0	41.0	34.0	41.0
29	37.55383388656695	39.0	35.0	41.0	34.0	41.0
30	37.442096822602394	40.0	35.0	41.0	33.0	41.0
31	37.241941827597174	40.0	35.0	41.0	33.0	41.0
32	37.007583108232204	40.0	35.0	41.0	33.0	41.0
33	36.66521078872747	39.0	35.0	41.0	32.0	41.0
34	36.36044402037333	39.0	35.0	41.0	31.0	41.0
35	36.19782200660297	39.0	35.0	41.0	30.0	41.0
36	36.11346171936945	39.0	35.0	41.0	30.0	41.0
37	36.04294167976957	39.0	35.0	41.0	30.0	41.0
38	35.966750435643476	39.0	35.0	41.0	29.0	41.0
39	35.895354181505425	39.0	35.0	41.0	29.0	41.0
40	35.822269108957904	39.0	35.0	41.0	29.0	41.0
41	35.74878803761093	39.0	35.0	41.0	28.0	41.0
42	35.64352761464479	39.0	35.0	41.0	27.0	41.0
43	35.530714544892554	38.0	35.0	41.0	27.0	41.0
44	35.4505794394198	38.0	35.0	40.0	26.0	41.0
45	35.40020785457347	38.0	35.0	40.0	26.0	41.0
46	35.30464313071992	38.0	35.0	40.0	25.0	41.0
47	35.16522109186366	38.0	35.0	40.0	24.0	41.0
48	35.13668588425546	38.0	35.0	40.0	24.0	41.0
49	35.079094936680505	38.0	35.0	40.0	24.0	41.0
50	34.97156065635457	38.0	35.0	40.0	24.0	41.0
51	34.56619585813925	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	9.0
15	5.0
16	46.0
17	81.0
18	200.0
19	397.0
20	663.0
21	1194.0
22	1911.0
23	3292.0
24	5889.0
25	11509.0
26	18031.0
27	20708.0
28	19157.0
29	17775.0
30	18439.0
31	20659.0
32	24957.0
33	32879.0
34	58524.0
35	100598.0
36	112091.0
37	83505.0
38	146025.0
39	417452.0
40	167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.19227443971097	18.31118159053545	25.52633347220169	29.970210497551886
2	35.22884161391909	19.241330806825154	25.259258263787167	20.27056931546859
3	20.451814919837123	20.758221230732016	39.165356376521395	19.62460747290947
4	21.89685216791424	23.890105853525238	37.0007122602841	17.212329718276422
5	19.91587265323675	28.252453714280595	33.003991345365606	18.82768228711705
6	71.26500114230423	1.5046162529733507	23.16162932899706	4.0687532757253635
7	72.00189936075759	1.3403036289437493	19.969717738864773	6.6880792714338835
8	61.06131261954998	3.2593747340222996	27.05307906985078	8.62623357657694
9	33.28692442425627	18.266474938741137	30.57782675500486	17.868773881997733
10	25.678730295252045	20.188144225988093	41.94559048169402	12.187534997065846
11	22.548010374810175	16.760604390927863	41.838527457857936	18.852857776404026
12	18.403551446246745	19.48564952314398	43.888314003753926	18.22248502685535
13	13.955015611491135	22.202900108854873	43.19209077510941	20.64999350454458
14	12.331062163748191	22.137766369667567	44.75798829026175	20.773183176322497
15	13.771171824954195	18.72626359006061	47.220348246003056	20.28221633898214
16	14.227735146685303	24.48688141986176	39.79178705657318	21.49359637687976
17	15.602890253681132	21.5162632764869	42.34768157037714	20.53316489945483
18	17.263576621736036	27.653886298172758	39.243301841573604	15.839235238517603
19	17.094963558255277	23.198003879354758	37.53226449494475	22.174768067445225
20	14.060645155510162	28.776569772390282	36.41737556723244	20.745409504867112
21	14.598916826813241	23.087715525930307	38.529608077658764	23.783759569597684
22	13.201901152607364	26.036293917117987	34.75310549963491	26.008699430639737
23	12.86763157776852	22.573006679120024	43.84978923367065	20.709572509440807
24	16.94426899248767	21.70189891279515	39.745198962518984	21.608633132198197
25	13.918730653622	25.27090528730071	39.33146085032231	21.47890320875498
26	14.965798067490022	22.975187360291713	37.711001509633434	24.348013062584833
27	18.678421201166493	23.20929253291404	38.30759789099282	19.804688374926645
28	14.573830930014827	22.881742394717627	35.423526091572484	27.12090058369506
29	15.734412026895663	27.03990897403162	34.03520088875749	23.190478110315233
30	17.93157821648233	23.928720216097084	32.506932218802774	25.632769348617813
31	16.78918439478034	25.46532098748841	31.88318931340796	25.862305304323286
32	14.561377574104187	30.82196628634655	30.62513158896758	23.99152455058168
33	12.878651453862108	25.05731679455994	35.167560351740114	26.89647139983784
34	12.65072816295082	29.45621839065013	32.462942306916986	25.430111139482065
35	18.40140122652117	26.532815488749424	33.483669529146674	21.582113755582732
36	14.320821742305126	32.10869360712798	33.41083083594271	20.159653814624182
37	17.115749015602532	27.83575904996125	36.71849592130196	18.32999601313426
38	17.12676889169612	29.619635089794073	32.071243946907494	21.182352071602317
39	21.13674949492235	27.212284922032136	34.15561319338987	17.495352389655654
40	15.524586418674657	24.690614738860294	37.79235148925114	21.992447353213905
41	18.22033480712977	25.918748572119714	31.549636478477645	24.311280142272874
42	17.936505803353448	22.10407959396684	36.15155465365783	23.80785994902187
43	20.26420824878042	24.097154094600707	34.652403542487	20.98623411413187
44	14.99034640935704	26.534338561055044	35.636577029381854	22.838738000206064
45	17.976553645742342	23.578861548247794	35.30168030712305	23.142904498886814
46	21.153503290284142	22.621386622945533	33.10200552785654	23.12310455891378
47	16.55033082026403	20.90578005939982	37.354602590118844	25.18928653021731
48	16.55749821934929	19.88388813481878	33.983685207832174	29.57492843799976
49	18.179659817320914	19.578825711252367	38.46366800607437	23.777846465352344
50	17.35890302957	19.005254599454382	39.23488014764842	24.4009622233272
51	15.365111789027608	18.965027572088356	37.73133900453786	27.938521634346174
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	319.0
1	1495.5
2	2672.0
3	44666.0
4	86660.0
5	48528.0
6	10396.0
7	9821.0
8	9246.0
9	9537.5
10	9829.0
11	9952.5
12	10076.0
13	9723.5
14	9371.0
15	8661.5
16	7952.0
17	7164.0
18	6376.0
19	5829.5
20	5283.0
21	4937.0
22	4591.0
23	4437.5
24	4284.0
25	4276.5
26	4981.0
27	5693.0
28	5913.0
29	6133.0
30	6878.5
31	7624.0
32	8681.5
33	9739.0
34	12944.5
35	16150.0
36	19301.5
37	22453.0
38	24690.5
39	26928.0
40	33208.0
41	39488.0
42	51718.0
43	63948.0
44	91075.5
45	118203.0
46	144160.0
47	170117.0
48	166277.5
49	162438.0
50	133558.0
51	104678.0
52	84619.0
53	64560.0
54	52247.0
55	39934.0
56	34100.5
57	28267.0
58	24469.5
59	20672.0
60	17035.0
61	13398.0
62	10914.0
63	8430.0
64	6736.0
65	5042.0
66	4156.0
67	3270.0
68	2699.5
69	2129.0
70	1806.5
71	1484.0
72	1433.0
73	1382.0
74	1309.5
75	904.0
76	571.0
77	545.5
78	520.0
79	341.5
80	163.0
81	120.5
82	78.0
83	64.0
84	50.0
85	43.0
86	36.0
87	21.5
88	7.0
89	10.0
90	13.0
91	8.0
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1116165.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.182524723381825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.4839449518293	25.46049289810916
2	11.794338235593958	8.06320516668236
3	4.408061060236893	4.520359685211728
4	2.191649999014865	2.996645211053015
5	1.2883740502366494	2.201993891258892
6	0.784993570780161	1.6099837284533176
7	0.5909324616578887	1.4139694436328762
8	0.4307768825498692	1.1780033150417801
9	0.351128985465629	1.0802227704097354
>10	3.2400406718583374	22.978220934871686
>50	0.2765810631788717	6.3634327422524954
>100	0.1464861286545046	8.848398896706353
>500	0.0074036310501040025	1.6737282759162555
>1k	0.004495061708991715	3.3636352623514116
>5k	5.28830789293143E-4	1.2967372056685667
>10k+	2.644153946465715E-4	6.950970572380366
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	76905	6.890110333149668	No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	7494	0.6714061093117953	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	6853	0.6139773241411439	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG	4809	0.43085027751273336	No Hit
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	4445	0.3982386116747972	Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTC	3446	0.3087357155976043	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTT	3049	0.2731674976369981	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCC	2439	0.21851607961188535	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCT	2426	0.21735137726053044	No Hit
TCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	2308	0.20677946360977095	Illumina Single End Adapter 2 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT	1993	0.17855782971155698	No Hit
GCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG	1715	0.15365111789027608	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTC	1690	0.15141130567613212	No Hit
ACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG	1607	0.14397512912517416	No Hit
TCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG	1392	0.12471274408353604	No Hit
AGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG	1317	0.11799330744110413	No Hit
ACTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1261	0.11297612808142164	Illumina Single End Adapter 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT	1223	0.10957161351592283	Illumina Single End Adapter 2 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6294768246630202	0.0
2	0.0	0.0	0.0	2.058297832309739	0.0
3	0.0	0.0	0.0	3.548041732181174	0.0
4	0.0	0.0	0.0	4.468962922148607	0.0
5	0.0	0.0	0.0	8.956023526987497	0.0
6	0.0	0.0	0.0	10.518785305040025	0.0
7	0.0	0.0	0.0	14.445624078877227	0.0
8	0.0	0.0	0.0	19.032849085932636	0.0
9	0.0	0.0	0.0	22.606066307400788	0.0
10	0.0	0.0	0.0	24.71059386381046	0.0
11	0.0	0.0	0.0	26.55019643153118	0.0
12	0.0	0.0	0.0	27.90949366805087	0.0
13	0.0	0.0	0.0	28.515407668221098	0.0
14	0.0	0.0	0.0	28.968297697921006	0.0
15	0.0	0.0	0.0	30.235762633660794	0.0
16	0.0	0.0	0.0	31.759372494210087	0.0
17	0.0	0.0	0.0	33.375083433004974	0.0
18	0.0	0.0	0.0	34.30496387182899	0.0
19	0.0	0.0	0.0	35.27534011548472	0.0
20	0.0	0.0	0.0	36.413164720269855	0.0
21	0.0	0.0	0.0	37.54785358795519	0.0
22	1.7918497713151728E-4	0.0	0.0	38.669999507241315	0.0
23	1.7918497713151728E-4	0.0	0.0	39.54594526794874	0.0
24	2.687774656972759E-4	0.0	0.0	40.316888632057086	0.0
25	2.687774656972759E-4	0.0	0.0	41.03371813307172	0.0
26	2.687774656972759E-4	0.0	0.0	41.765240802211146	0.0
27	2.687774656972759E-4	0.0	0.0	42.471408797086454	0.0
28	4.479624428287932E-4	0.0	0.0	43.166736100845306	0.0
29	4.479624428287932E-4	0.0	0.0	43.97871282471677	0.0
30	4.479624428287932E-4	0.0	0.0	44.69778213794555	0.0
31	4.479624428287932E-4	0.0	0.0	45.32322730062312	0.0
32	4.479624428287932E-4	0.0	0.0	45.96363440889116	0.0
33	4.479624428287932E-4	0.0	0.0	46.5781492879637	0.0
34	4.479624428287932E-4	0.0	0.0	47.30931358714885	0.0
35	4.479624428287932E-4	0.0	0.0	47.89435253748326	0.0
36	4.479624428287932E-4	0.0	0.0	48.468909166655465	0.0
37	4.479624428287932E-4	0.0	0.0	49.06039877616661	0.0
38	4.479624428287932E-4	0.0	0.0	49.620262237214035	0.0
39	4.479624428287932E-4	0.0	0.0	50.176631591207396	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	115	0.0	45.000004	5
CCTACGG	30	2.165445E-6	45.000004	1
AGGGTCG	30	2.165445E-6	45.000004	5
GTCACGG	110	0.0	45.000004	1
ACTACGG	60	0.0	45.000004	1
AGCCGTG	20	7.0331997E-4	45.0	24
GATACGA	20	7.0331997E-4	45.0	10
CAGCCGT	20	7.0331997E-4	45.0	23
CGGCCAC	35	1.2118835E-7	45.0	22
TGCGGTA	45	3.8562575E-10	45.0	21
TTATCGG	35	1.2118835E-7	45.0	1
GCACCGG	40	6.8139343E-9	45.0	1
ATGTACG	20	7.0331997E-4	45.0	11
CTAACGG	45	3.8562575E-10	45.0	1
CTACGGC	45	3.8562575E-10	45.0	2
ACGTGCG	45	3.8562575E-10	45.0	18
TCCACCG	35	1.2118835E-7	45.0	30
ACCGTTC	20	7.0331997E-4	45.0	9
TGAGGCG	20	7.0331997E-4	45.0	3
GACGAGC	20	7.0331997E-4	45.0	1
>>END_MODULE