FastQCFastQC Report
Thu 26 May 2016
SRR1043320_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043320_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences844963
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT589556.977228588707435No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT88271.044661127173616Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTT80080.9477338060956516Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT55310.6545848753140671Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC33800.4000175155598529Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT30930.3660515312504808Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG27600.32664152158141835No Hit
ACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC24580.29090031161127766Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC19990.23657840639175917Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC18640.22060137544484196Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT16660.19716839672269676No Hit
ACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT16390.1939729905333133Illumina Single End Adapter 2 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC15880.18793722328670012Illumina Single End Adapter 2 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC13950.16509598645147774Illumina Single End Adapter 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTTG12250.14497676229610054Illumina Single End Adapter 2 (95% over 24bp)
GGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC11930.14118961421979423Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT11150.13195844078379762No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCC11140.13184009240641306No Hit
ATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC11080.13113000214210563Illumina Single End Adapter 2 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG10880.12876303459441418No Hit
CGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG9560.1131410487796507No Hit
GTACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT9430.11160251987365127No Hit
ATAGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT9280.10982729421288269No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTT9230.10923555232595983No Hit
ATGCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT9060.1072236299104221No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9020.10675023640088381No Hit
ATTGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT8900.10533005587226897No Hit
GTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC8830.10450161723057697Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGT8820.10438326885319239No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAC351.0191434E-746.0000047
AATCAGG351.0191434E-746.0000041
GCAACGG351.0191434E-746.0000041
ACGGGTA351.0191434E-746.0000044
GAGACGG351.0191434E-746.0000041
CACTAGG351.0191434E-746.0000041
GGCCTAT351.0191434E-746.0000047
TTGGGCG700.046.0000044
TGGTAGT351.0191434E-746.00000445
GGCTAAC351.0191434E-746.0000048
TGATCGG253.4163826E-546.01
TCACGGC253.4163826E-546.02
TCACGAC600.046.024
CTTAATA206.3105015E-446.012
ATTTACT453.092282E-1046.014
GTCATCC206.3105015E-446.016
TGTCAGA206.3105015E-446.043
AGCGTGT206.3105015E-446.024
CCTTAGC253.4163826E-546.042
ATCTCGG551.8189894E-1246.01
Produced by FastQC (version 0.11.5)