##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043320_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	844963
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19080835492205	33.0	31.0	34.0	30.0	34.0
2	32.50929093936658	34.0	31.0	34.0	31.0	34.0
3	32.56866750378419	34.0	31.0	34.0	31.0	34.0
4	36.102670767832436	37.0	35.0	37.0	35.0	37.0
5	35.986046726306355	37.0	35.0	37.0	35.0	37.0
6	36.259614918049664	37.0	36.0	37.0	35.0	37.0
7	36.17316024488646	37.0	37.0	37.0	35.0	37.0
8	36.15487068664545	37.0	37.0	37.0	35.0	37.0
9	37.739230001787064	39.0	38.0	39.0	35.0	39.0
10	37.55908601915113	39.0	37.0	39.0	35.0	39.0
11	37.20260177072842	39.0	37.0	39.0	34.0	39.0
12	37.164652180036285	39.0	37.0	39.0	34.0	39.0
13	37.1705613145191	39.0	37.0	39.0	34.0	39.0
14	38.559774806707516	40.0	38.0	41.0	34.0	41.0
15	38.67309574502079	40.0	38.0	41.0	35.0	41.0
16	38.69908268172689	40.0	38.0	41.0	35.0	41.0
17	38.70240235371253	40.0	38.0	41.0	35.0	41.0
18	38.41455661372155	40.0	37.0	41.0	35.0	41.0
19	38.15084329136305	40.0	37.0	41.0	34.0	41.0
20	37.82533318026943	40.0	35.0	41.0	34.0	41.0
21	37.76277067753263	39.0	35.0	41.0	34.0	41.0
22	37.722751173720035	39.0	35.0	41.0	34.0	41.0
23	37.73238118118782	39.0	35.0	41.0	34.0	41.0
24	37.63775573604998	39.0	35.0	41.0	34.0	41.0
25	37.55428462548064	39.0	35.0	41.0	34.0	41.0
26	37.50756897047563	39.0	35.0	41.0	34.0	41.0
27	37.54179532121525	39.0	35.0	41.0	34.0	41.0
28	37.523800450433924	39.0	35.0	41.0	34.0	41.0
29	37.50620323020061	39.0	35.0	41.0	34.0	41.0
30	37.36265729978709	39.0	35.0	41.0	33.0	41.0
31	37.293701617703974	39.0	35.0	41.0	33.0	41.0
32	37.10836450826841	39.0	35.0	41.0	33.0	41.0
33	36.91014872840586	39.0	35.0	41.0	33.0	41.0
34	36.75336553198187	39.0	35.0	41.0	32.0	41.0
35	36.66389770913046	40.0	35.0	41.0	32.0	41.0
36	36.63470826533233	40.0	35.0	41.0	32.0	41.0
37	36.61041844435792	39.0	35.0	41.0	32.0	41.0
38	36.59582727291018	39.0	35.0	41.0	32.0	41.0
39	36.58556883555848	40.0	35.0	41.0	31.0	41.0
40	36.50927555407752	39.0	35.0	41.0	31.0	41.0
41	36.49706555198275	39.0	35.0	41.0	31.0	41.0
42	36.39175206488331	39.0	35.0	41.0	31.0	41.0
43	36.26772769931938	39.0	35.0	41.0	31.0	41.0
44	36.120528354495995	39.0	35.0	41.0	31.0	41.0
45	36.027998859121645	39.0	35.0	41.0	30.0	41.0
46	36.008799201858544	39.0	35.0	41.0	30.0	41.0
47	35.890855575924625	38.0	35.0	41.0	30.0	41.0
48	35.87605137739759	38.0	35.0	41.0	30.0	41.0
49	35.83758578778006	38.0	35.0	41.0	30.0	41.0
50	35.67802022100376	38.0	35.0	40.0	29.0	41.0
51	35.597900736481954	38.0	35.0	40.0	29.0	41.0
52	35.252312823165035	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	4.0
15	7.0
16	24.0
17	57.0
18	134.0
19	253.0
20	539.0
21	826.0
22	1411.0
23	2345.0
24	4335.0
25	7478.0
26	10257.0
27	11096.0
28	10747.0
29	10877.0
30	12667.0
31	15301.0
32	19171.0
33	25514.0
34	45207.0
35	92043.0
36	88983.0
37	63156.0
38	101871.0
39	319510.0
40	1149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.27618014043219	19.294454313384136	25.32347570248638	29.105889843697298
2	33.65058588364224	18.977635707125636	26.84176703595305	20.530011373279066
3	20.553562700378595	21.450525052576268	39.095794727106394	18.900117519938743
4	22.890351411836967	23.528367514317196	36.446921344484906	17.13435972936093
5	19.695181919208295	28.496514048544135	31.703991772420803	20.104312259826763
6	69.5164166951689	1.5710747097801916	24.047325149148545	4.865183445902365
7	70.24520600310309	1.55213896939866	19.206876514119553	8.995778513378692
8	55.84445709457101	3.0455771436145724	29.68200974480539	11.427956017009029
9	32.68249615663644	17.15944958536646	30.859339403027114	19.29871485496998
10	26.363639591319384	17.279218143279646	44.660653780106344	11.696488485294623
11	22.498854979448804	15.418426605662022	40.758589429359624	21.324128985529544
12	15.843889022359559	21.272884138122024	45.053688741400514	17.829538098117904
13	10.976693654041657	24.732562254205213	44.66586110871127	19.62488298304186
14	9.214249618030612	22.687857338131963	47.547407401270824	20.5504856425666
15	9.383724494445318	17.991912071889537	52.086896112610845	20.537467321054294
16	9.252831189057982	25.59165312563982	39.931689316573625	25.22382636872857
17	11.914486196437004	22.7151958133078	42.6159488640331	22.754369126222095
18	14.003335057274697	29.6635473979334	40.801431541972846	15.53168600281906
19	14.188786964636321	23.478661195815675	36.53781289831626	25.79473894123175
20	11.122143809847296	31.48623075803319	35.99956447797123	21.39206095414829
21	13.205193600193144	24.3698244775215	36.75569226108125	25.669289661204097
22	10.423770034900937	27.676359793269057	31.573335163788236	30.326535008041773
23	10.06481940629353	23.912407998930128	44.23779502771127	21.784977567065066
24	16.493503265823474	20.820911684890344	39.86482248335134	22.820762565934842
25	11.774953459500594	26.503172328255793	38.06438861819985	23.657485594043763
26	15.598671184418725	22.781352556265777	36.41047004425046	25.209506215065037
27	21.930664419625476	24.089338823120066	37.85751565453162	16.12248110272284
28	13.698114591999886	24.222599096055095	35.47220410834557	26.607082203599447
29	21.5844954157756	22.75365903595779	33.64466846477301	22.017177083493596
30	20.423852878765107	27.94027667483665	30.682763623969333	20.95310682242891
31	22.27150774649304	20.8772455125254	36.285020764222814	20.566225976758744
32	21.123883530994846	24.306863140752906	35.37551348402238	19.193739844229864
33	16.626053448494194	28.184784422513175	35.31953470151947	19.869627427473155
34	14.60111271144417	19.620030699569092	46.18415244217794	19.594704146808795
35	22.680164693601967	22.215410615612754	39.17994042342682	15.924484267358451
36	15.212973822522407	24.87256838465116	38.616365450321496	21.298092342504937
37	17.97486990554616	23.467773145096295	43.18887335895181	15.368483590405734
38	18.394888296884005	19.84465591984501	36.348100449368786	25.41235533390219
39	24.813275847581494	17.770008864293466	35.55682319817554	21.8598920899495
40	16.156447087032213	16.92630328191885	41.17564911126286	25.741600519786072
41	21.39868846328182	20.933579340160456	32.26271446205337	25.405017734504355
42	20.435924413258334	16.094787582414853	39.6442211079065	23.825066896420317
43	22.97485215328955	20.22928814634487	36.935345097951036	19.860514602414543
44	14.886450649318373	23.99501516634456	38.572576550689206	22.545957633647866
45	19.640741665611394	20.761619147820674	37.00528898898532	22.59235019758262
46	25.166545754074438	16.407345647087507	36.20679248677161	22.219316112066444
47	17.370583090620535	15.719149832596221	40.726635367465796	26.18363170931745
48	17.88610862250773	13.784863952622779	34.660689284619565	33.66833814024993
49	20.783750294391588	14.134820104548956	41.742182793802804	23.33924680725665
50	18.531343976008415	14.045822124755759	41.20121236077793	26.221621538457896
51	15.735600257052676	13.204128464796682	39.43888667314427	31.621384605006376
52	14.673186873271375	12.798785272254525	50.22208073016215	22.305947124311952
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	416.0
1	1643.0
2	2870.0
3	32870.0
4	62870.0
5	33887.0
6	4904.0
7	4758.5
8	4613.0
9	5041.5
10	5470.0
11	5770.5
12	6071.0
13	5839.0
14	5313.0
15	5019.0
16	4512.0
17	4005.0
18	3706.5
19	3408.0
20	3244.5
21	3081.0
22	2787.5
23	2494.0
24	2569.0
25	2644.0
26	2674.5
27	2705.0
28	2705.0
29	2705.0
30	3333.0
31	3961.0
32	4523.0
33	5085.0
34	5910.0
35	6735.0
36	7934.5
37	9134.0
38	10995.5
39	16930.0
40	21003.0
41	33023.0
42	45043.0
43	67341.0
44	89639.0
45	118794.5
46	147950.0
47	149722.5
48	151495.0
49	125314.0
50	99133.0
51	76608.5
52	54084.0
53	40487.5
54	26891.0
55	21538.5
56	16186.0
57	13855.0
58	11524.0
59	9948.5
60	8373.0
61	6857.5
62	5342.0
63	4559.0
64	3102.5
65	2429.0
66	1906.5
67	1384.0
68	1277.0
69	1170.0
70	980.0
71	790.0
72	897.0
73	1004.0
74	728.0
75	452.0
76	358.5
77	265.0
78	270.0
79	275.0
80	171.5
81	68.0
82	43.0
83	18.0
84	13.5
85	9.0
86	6.0
87	3.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	844963.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.758868514793505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.43306896641029	19.114681000803493
2	11.870736818510458	6.352949714004791
3	5.032732806958736	4.040107063544894
4	2.739449971640493	2.9321832637593097
5	1.7085638927833788	2.285961827805709
6	1.1351330966531645	1.8224926368079448
7	0.7508308261700203	1.4063968348036144
8	0.5605293825983003	1.1999305638101039
9	0.4407087216555566	1.061358006249339
>10	3.4711853165780293	20.25411881554878
>50	0.5591065247395032	10.100909058224687
>100	0.2744543526964083	13.675901911720215
>500	0.014631653697869907	2.7014141880483535
>1k	0.007094135126239955	3.403314418031842
>5k	0.0013301503361699916	2.6536004976015404
>10k+	4.433834453899972E-4	6.994680199235394
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58955	6.977228588707435	No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	8827	1.044661127173616	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTT	8008	0.9477338060956516	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	5531	0.6545848753140671	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	3380	0.4000175155598529	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	3093	0.3660515312504808	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG	2760	0.32664152158141835	No Hit
ACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	2458	0.29090031161127766	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1999	0.23657840639175917	Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1864	0.22060137544484196	Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	1666	0.19716839672269676	No Hit
ACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	1639	0.1939729905333133	Illumina Single End Adapter 2 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1588	0.18793722328670012	Illumina Single End Adapter 2 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1395	0.16509598645147774	Illumina Single End Adapter 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTTG	1225	0.14497676229610054	Illumina Single End Adapter 2 (95% over 24bp)
GGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1193	0.14118961421979423	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT	1115	0.13195844078379762	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCC	1114	0.13184009240641306	No Hit
ATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1108	0.13113000214210563	Illumina Single End Adapter 2 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	1088	0.12876303459441418	No Hit
CGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	956	0.1131410487796507	No Hit
GTACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	943	0.11160251987365127	No Hit
ATAGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	928	0.10982729421288269	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTT	923	0.10923555232595983	No Hit
ATGCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	906	0.1072236299104221	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	902	0.10675023640088381	No Hit
ATTGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	890	0.10533005587226897	No Hit
GTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	883	0.10450161723057697	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGT	882	0.10438326885319239	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9731787072333344	0.0
2	0.0	0.0	0.0	3.2832206854027928	0.0
3	0.0	0.0	0.0	5.444972146709382	0.0
4	0.0	0.0	0.0	6.777929921191816	0.0
5	0.0	0.0	0.0	13.406385841746916	0.0
6	0.0	0.0	0.0	15.016515516064016	0.0
7	0.0	0.0	0.0	20.373081424867124	0.0
8	0.0	0.0	0.0	27.096926137594192	0.0
9	0.0	0.0	0.0	31.81748786633261	0.0
10	0.0	0.0	0.0	34.640570060464185	0.0
11	0.0	0.0	0.0	37.24376096941523	0.0
12	0.0	0.0	0.0	39.11804422205469	0.0
13	0.0	0.0	0.0	39.824228989908434	0.0
14	0.0	0.0	0.0	40.314901362544866	0.0
15	0.0	0.0	0.0	41.97982633559103	0.0
16	0.0	0.0	0.0	43.85091418204111	0.0
17	0.0	0.0	0.0	46.10805443551966	0.0
18	0.0	0.0	0.0	47.11164867574083	0.0
19	0.0	0.0	0.0	48.284836140754095	0.0
20	1.1834837738457187E-4	0.0	0.0	49.458141954144736	0.0
21	1.1834837738457187E-4	0.0	0.0	50.466825174593446	0.0
22	5.917418869228594E-4	0.0	0.0	51.53941651882982	0.0
23	7.100902643074312E-4	0.0	0.0	52.31980571930369	0.0
24	0.006390812378766881	0.0	0.0	52.938175991138074	0.0
25	0.006509160756151453	0.0	0.0	53.516189466284324	0.0
26	0.006509160756151453	0.0	0.0	54.08331489071119	0.0
27	0.006509160756151453	0.0	0.0	54.59469822938993	0.0
28	0.006509160756151453	0.0	0.0	55.128212714639574	0.0
29	0.006509160756151453	0.0	0.0	55.77818200323564	0.0
30	0.006509160756151453	0.0	0.0	56.37347434148004	0.0
31	0.006509160756151453	0.0	0.0	56.870892571627394	0.0
32	0.006509160756151453	0.0	0.0	57.354108996488605	0.0
33	0.006509160756151453	0.0	0.0	57.79779706330336	0.0
34	0.006509160756151453	0.0	0.0	58.35344269512393	0.0
35	0.006509160756151453	0.0	0.0	58.77961520208577	0.0
36	0.006627509133536024	0.0	0.0	59.191585903761464	0.0
37	0.006627509133536024	0.0	0.0	59.62083546853531	0.0
38	0.006627509133536024	0.0	0.0	60.036474969909925	0.0
39	0.007100902643074312	0.0	0.0	60.44832732320823	0.0
40	0.007100902643074312	0.0	0.0	60.872606256131924	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACAC	35	1.0191434E-7	46.000004	7
AATCAGG	35	1.0191434E-7	46.000004	1
GCAACGG	35	1.0191434E-7	46.000004	1
ACGGGTA	35	1.0191434E-7	46.000004	4
GAGACGG	35	1.0191434E-7	46.000004	1
CACTAGG	35	1.0191434E-7	46.000004	1
GGCCTAT	35	1.0191434E-7	46.000004	7
TTGGGCG	70	0.0	46.000004	4
TGGTAGT	35	1.0191434E-7	46.000004	45
GGCTAAC	35	1.0191434E-7	46.000004	8
TGATCGG	25	3.4163826E-5	46.0	1
TCACGGC	25	3.4163826E-5	46.0	2
TCACGAC	60	0.0	46.0	24
CTTAATA	20	6.3105015E-4	46.0	12
ATTTACT	45	3.092282E-10	46.0	14
GTCATCC	20	6.3105015E-4	46.0	16
TGTCAGA	20	6.3105015E-4	46.0	43
AGCGTGT	20	6.3105015E-4	46.0	24
CCTTAGC	25	3.4163826E-5	46.0	42
ATCTCGG	55	1.8189894E-12	46.0	1
>>END_MODULE