FastQCFastQC Report
Thu 26 May 2016
SRR1043319_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043319_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences832807
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT551416.621101887952431No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC85541.0271287345087157Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT77840.9346703377853453Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC55350.6646197738491632Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG34670.4163029369349681No Hit
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC28640.343897205474978Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCC26910.32312408517219476No Hit
ACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG24600.2953865661551836No Hit
GCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG21270.2554013114683234No Hit
AGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG19120.22958500588971995No Hit
ACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC16020.19236149552057077Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC15520.18635770352554673No Hit
TCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG15000.1801137598507217No Hit
TGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTT13470.1617421563459481Illumina Single End Adapter 2 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG12700.15249631667361105No Hit
GGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG12270.14733305555789036No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTC10660.1280008453339129No Hit
CGTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT10610.1274004661344105No Hit
CGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT10330.12403834261719701No Hit
ATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG10140.12175690165908788No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC10010.12019591574038162No Hit
GTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG9220.11070992438824362No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT9170.1101095451887412No Hit
ATGCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC9050.10866863510993544No Hit
ATTGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC8870.10650726999172679No Hit
GTACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC8740.10494628407302052No Hit
ATAGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC8470.10170423639570753No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG8340.10014325047700127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA650.045.00000416
CGCGGCT302.1647793E-645.0000043
GCTATCA351.2113924E-745.00000442
TTGTAGG1550.045.0000041
TTGTAGC302.1647793E-645.0000041
CTTGCGG1200.045.0000041
ATGCGGC351.2113924E-745.0000042
CGATGTA302.1647793E-645.0000049
TAAGGGT950.045.0000043
TGGGCGC351.2113924E-745.0000045
AGCTATC351.2113924E-745.00000441
TATACGG650.045.0000041
GCGTAGG1200.045.0000041
ACCCACG351.2113924E-745.00000422
TGCACGG650.045.0000041
GAGATGT302.1647793E-645.0000048
AGCACGG600.045.0000041
TCACGGC207.0321665E-445.02
AGCGTGT207.0321665E-445.024
GGTACCC207.0321665E-445.028
Produced by FastQC (version 0.11.5)