##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043319_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	832807
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10282694549878	33.0	31.0	34.0	30.0	34.0
2	32.3929722012423	34.0	31.0	34.0	30.0	34.0
3	32.445811574590515	34.0	31.0	34.0	31.0	34.0
4	36.04107674407155	37.0	35.0	37.0	35.0	37.0
5	35.98955220117026	37.0	35.0	37.0	35.0	37.0
6	36.26032442090424	37.0	37.0	37.0	35.0	37.0
7	36.1849648237827	37.0	37.0	37.0	35.0	37.0
8	36.08805641643262	37.0	36.0	37.0	35.0	37.0
9	37.67161299076497	39.0	38.0	39.0	35.0	39.0
10	37.48590729905008	39.0	37.0	39.0	35.0	39.0
11	37.17897063785487	39.0	37.0	39.0	34.0	39.0
12	37.1429767040863	39.0	37.0	39.0	33.0	39.0
13	37.17371131606723	39.0	37.0	39.0	34.0	39.0
14	38.554931694858475	40.0	38.0	41.0	34.0	41.0
15	38.6916092203836	40.0	38.0	41.0	35.0	41.0
16	38.740223124925706	40.0	38.0	41.0	35.0	41.0
17	38.72353018166274	40.0	38.0	41.0	35.0	41.0
18	38.47691481939993	40.0	38.0	41.0	35.0	41.0
19	38.230430339802616	40.0	37.0	41.0	35.0	41.0
20	37.94245965751969	40.0	35.0	41.0	34.0	41.0
21	37.879182091408936	40.0	35.0	41.0	34.0	41.0
22	37.85015856014659	40.0	35.0	41.0	34.0	41.0
23	37.82315350375297	40.0	35.0	41.0	34.0	41.0
24	37.742714698603635	39.0	35.0	41.0	34.0	41.0
25	37.71905855738484	39.0	35.0	41.0	34.0	41.0
26	37.710016846640336	40.0	35.0	41.0	34.0	41.0
27	37.70223953448998	40.0	35.0	41.0	34.0	41.0
28	37.65568012756857	40.0	35.0	41.0	34.0	41.0
29	37.66594901339686	40.0	35.0	41.0	34.0	41.0
30	37.469478522634894	39.0	35.0	41.0	34.0	41.0
31	37.39217489766536	40.0	35.0	41.0	33.0	41.0
32	37.21640908397744	40.0	35.0	41.0	33.0	41.0
33	36.97613132454458	39.0	35.0	41.0	33.0	41.0
34	36.79206106576914	39.0	35.0	41.0	32.0	41.0
35	36.70601351813806	40.0	35.0	41.0	32.0	41.0
36	36.652105469814735	39.0	35.0	41.0	32.0	41.0
37	36.592234455281954	39.0	35.0	41.0	32.0	41.0
38	36.56399501925416	39.0	35.0	41.0	31.0	41.0
39	36.502624257481024	39.0	35.0	41.0	31.0	41.0
40	36.40556935760626	39.0	35.0	41.0	31.0	41.0
41	36.352950923803476	39.0	35.0	41.0	31.0	41.0
42	36.22161437163712	39.0	35.0	41.0	31.0	41.0
43	36.08006416852884	39.0	35.0	41.0	30.0	41.0
44	35.97774514383284	38.0	35.0	41.0	30.0	41.0
45	35.9198817973432	38.0	35.0	41.0	30.0	41.0
46	35.84561489036476	38.0	35.0	40.0	29.0	41.0
47	35.67285457494954	38.0	35.0	40.0	28.0	41.0
48	35.689157271732824	38.0	35.0	40.0	29.0	41.0
49	35.637240080835056	38.0	35.0	40.0	29.0	41.0
50	35.5209922587106	38.0	35.0	40.0	28.0	41.0
51	35.075802676970774	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	6.0
16	20.0
17	48.0
18	120.0
19	247.0
20	441.0
21	754.0
22	1303.0
23	1968.0
24	3714.0
25	6548.0
26	9649.0
27	11277.0
28	11122.0
29	11148.0
30	12808.0
31	15138.0
32	19006.0
33	24958.0
34	45516.0
35	89729.0
36	84659.0
37	63566.0
38	107943.0
39	310995.0
40	122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.733444843763323	19.331009465578457	25.384753010001116	28.550792680657107
2	33.447845659318425	19.060718749962476	26.83526915599893	20.65616643472017
3	20.7889703136501	21.566461377005716	38.59453630913285	19.050032000211335
4	23.046035876259445	23.741635216802933	35.98877050745251	17.223558399485114
5	19.899688643347137	28.704129528210014	31.15787931657635	20.238302511866497
6	70.06569349200956	1.5426143152014813	23.559960471033506	4.83173172175546
7	70.76945798966628	1.4985464819580048	18.629046105520246	9.102949422855476
8	56.122847190285384	2.93741527148547	29.312673884825657	11.62706365340349
9	33.01269081551908	17.0211105334129	30.411968199114558	19.55423045195345
10	26.62645727041199	17.26186259241337	44.3715050425849	11.740175094589743
11	22.59719238671145	15.28337297837314	40.59944260795118	21.51999202696423
12	16.063505710206567	21.144274723915625	44.822629973091004	17.969589592786804
13	11.078917444257794	24.676065402908478	44.43106265917554	19.813954493658194
14	9.187002510785812	22.5606893313817	47.39729613223712	20.855012025595364
15	9.387649239259515	17.809408422359564	52.054917886136884	20.74802445224404
16	9.222665035236254	25.535688340756025	39.83636064538362	25.405285978624097
17	11.951988876174191	22.688930328395415	42.3752442042394	22.983836591190997
18	13.972505034179589	29.807386345215637	40.71147336657833	15.508635254026442
19	14.19776730983289	23.539307426570623	36.256779782110385	26.006145481486108
20	11.071472742183964	31.640464117136386	35.62458048503434	21.663482655645304
21	13.131253699836817	24.476619432833775	36.4113173880623	25.9808094792671
22	10.411656001930819	27.715545138309356	31.0463288613088	30.826469998451024
23	10.098498211470366	23.93039443712649	43.8975657024977	22.073541648905447
24	16.55113369604242	20.832317691854175	39.54121423090824	23.075334381195162
25	11.685300435755224	26.479604518213705	37.95609306838199	23.879001977649082
26	15.702917962985422	22.563691227379213	36.22556006373625	25.507830745899106
27	22.17920838801787	24.029457005044385	37.49488176732424	16.2964528396135
28	13.842342823727465	24.240910559109132	35.01915810025612	26.897588516907277
29	21.878418409067166	22.5355934808425	33.225345127982834	22.3606429821075
30	20.65592628304037	27.79611602688258	30.22957299830573	21.31838469177132
31	22.5101374027836	20.760152112073985	35.765789672757315	20.963920812385105
32	21.38262526611808	24.159018836297005	34.97617094957175	19.482184948013163
33	16.930693425967842	28.163067793618453	34.946031913756734	19.96020686665698
34	14.818319250438577	19.551588783475644	45.933811795530055	19.69628017055572
35	22.786792137914308	22.154352689158472	39.16525677617984	15.893598396747386
36	15.223455134262801	24.787735934015924	38.56763932099514	21.421169610726135
37	18.1673545011029	23.192648476777933	43.196322797478885	15.443674224640283
38	18.5923028985107	19.638883919083295	36.155555849074275	25.613257333331735
39	24.990303875928035	17.607681011326754	35.35248863181985	22.04952648092535
40	16.207716793927045	16.627141702699426	41.153712684931804	26.01142881844173
41	21.705509199610475	20.678620616781558	32.00069163683782	25.61517854677014
42	20.59925048660734	15.871264290525897	39.48285737271661	24.046627850150156
43	23.192648476777933	20.091689911348006	36.70082023806236	20.0148413738117
44	15.039498947535263	23.90025540131147	38.42931195342978	22.63093369772348
45	19.84517421203232	20.505471255645066	37.0090549190869	22.64029961323572
46	25.533166748118113	16.157284941168843	36.033078492375786	22.27646981833726
47	17.740244738576884	15.45544165695053	40.41728755882215	26.38702604565043
48	18.098310893160118	13.442370201018964	34.30134472933105	34.15797417648987
49	21.001624626113855	13.772218533225585	41.540597041091154	23.685559799569408
50	18.70481396049745	13.662589291396445	41.16692102732086	26.46567572078525
51	15.970567010123593	12.845713352553473	39.15312911634989	32.03059052097304
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	346.0
1	1473.5
2	2601.0
3	30733.5
4	58866.0
5	31969.0
6	5072.0
7	4784.0
8	4496.0
9	4914.0
10	5332.0
11	5458.5
12	5585.0
13	5366.5
14	5148.0
15	4833.0
16	4518.0
17	4107.0
18	3696.0
19	3372.0
20	3048.0
21	2871.5
22	2695.0
23	2587.0
24	2479.0
25	2294.5
26	2314.5
27	2519.0
28	2647.5
29	2776.0
30	3391.0
31	4006.0
32	4921.0
33	5836.0
34	6524.0
35	7212.0
36	9090.5
37	10969.0
38	13171.5
39	15374.0
40	22116.5
41	28859.0
42	41484.0
43	54109.0
44	80294.5
45	106480.0
46	133595.5
47	160711.0
48	153530.0
49	146349.0
50	112308.0
51	78267.0
52	59141.5
53	40016.0
54	29925.5
55	19835.0
56	16935.5
57	14036.0
58	11909.0
59	9782.0
60	7955.5
61	6129.0
62	5321.5
63	4514.0
64	3663.5
65	2813.0
66	2274.0
67	1735.0
68	1483.5
69	1232.0
70	1112.0
71	992.0
72	944.5
73	897.0
74	737.5
75	436.5
76	295.0
77	329.0
78	363.0
79	213.5
80	64.0
81	51.0
82	38.0
83	28.0
84	18.0
85	12.0
86	6.0
87	4.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	832807.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.16826248988793
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.54910608618924	18.461475264903502
2	12.1178851158149	6.342079970659005
3	5.248145495895662	4.1200454676516225
4	2.9112734212752374	3.0473186827105794
5	1.6673752914029518	2.1816157147292907
6	1.1343854515468625	1.7810937756473009
7	0.7834104952106312	1.435034403320342
8	0.5731742959847607	1.1999180343828746
9	0.48261880343277985	1.1366365977706148
>10	3.6723671361835075	21.111386618476764
>50	0.5511391946376596	9.78746307350948
>100	0.28598154682503085	14.102423440388382
>500	0.013419846048423776	2.4323041544903825
>1k	0.00786680630424842	3.535232872955901
>5k	0.001388259936043839	2.648700028436132
>10k+	4.6275331201461303E-4	6.677271899967849
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55141	6.621101887952431	No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	8554	1.0271287345087157	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	7784	0.9346703377853453	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	5535	0.6646197738491632	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	3467	0.4163029369349681	No Hit
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	2864	0.343897205474978	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCC	2691	0.32312408517219476	No Hit
ACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	2460	0.2953865661551836	No Hit
GCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	2127	0.2554013114683234	No Hit
AGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	1912	0.22958500588971995	No Hit
ACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1602	0.19236149552057077	Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	1552	0.18635770352554673	No Hit
TCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	1500	0.1801137598507217	No Hit
TGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTT	1347	0.1617421563459481	Illumina Single End Adapter 2 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	1270	0.15249631667361105	No Hit
GGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	1227	0.14733305555789036	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTC	1066	0.1280008453339129	No Hit
CGTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	1061	0.1274004661344105	No Hit
CGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	1033	0.12403834261719701	No Hit
ATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	1014	0.12175690165908788	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC	1001	0.12019591574038162	No Hit
GTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	922	0.11070992438824362	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT	917	0.1101095451887412	No Hit
ATGCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	905	0.10866863510993544	No Hit
ATTGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	887	0.10650726999172679	No Hit
GTACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	874	0.10494628407302052	No Hit
ATAGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	847	0.10170423639570753	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG	834	0.10014325047700127	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9577248990462376	0.0
2	0.0	0.0	0.0	3.2454098008302044	0.0
3	0.0	0.0	0.0	5.438715092452393	0.0
4	0.0	0.0	0.0	6.78560578861609	0.0
5	1.2007583990048115E-4	0.0	0.0	13.49160129537816	0.0
6	1.2007583990048115E-4	0.0	0.0	15.109263010517443	0.0
7	1.2007583990048115E-4	0.0	0.0	20.58400085493998	0.0
8	1.2007583990048115E-4	0.0	0.0	27.496766958010678	0.0
9	1.2007583990048115E-4	0.0	0.0	32.39069796483459	0.0
10	1.2007583990048115E-4	0.0	0.0	35.351528025100656	0.0
11	1.2007583990048115E-4	0.0	0.0	38.037384411994616	0.0
12	1.2007583990048115E-4	0.0	0.0	39.92557699442968	0.0
13	1.2007583990048115E-4	0.0	0.0	40.650354764068986	0.0
14	1.2007583990048115E-4	0.0	0.0	41.15479336749091	0.0
15	1.2007583990048115E-4	0.0	0.0	42.84113846305326	0.0
16	1.2007583990048115E-4	0.0	0.0	44.74938371075171	0.0
17	1.2007583990048115E-4	0.0	0.0	47.04979665156513	0.0
18	1.2007583990048115E-4	0.0	0.0	48.07332311087683	0.0
19	1.2007583990048115E-4	0.0	0.0	49.27384135820184	0.0
20	2.401516798009623E-4	0.0	0.0	50.46463346249491	0.0
21	2.401516798009623E-4	0.0	0.0	51.46522543638562	0.0
22	8.40530879303368E-4	0.0	0.0	52.56283868891592	0.0
23	8.40530879303368E-4	0.0	0.0	53.37094909144616	0.0
24	0.006724247034426944	0.0	0.0	54.008912028837415	0.0
25	0.006964398714227906	0.0	0.0	54.61601547537425	0.0
26	0.006964398714227906	0.0	0.0	55.18157268130551	0.0
27	0.006964398714227906	0.0	0.0	55.7015010680746	0.0
28	0.007444702073829831	0.0	0.0	56.23583855563174	0.0
29	0.007444702073829831	0.0	0.0	56.90658219731583	0.0
30	0.007444702073829831	0.0	0.0	57.51872882912848	0.0
31	0.007444702073829831	0.0	0.0	58.01704356471547	0.0
32	0.007444702073829831	0.0	0.0	58.50323064047252	0.0
33	0.007444702073829831	0.0	0.0	58.95255443338012	0.0
34	0.007444702073829831	0.0	0.0	59.51258815067597	0.0
35	0.007444702073829831	0.0	0.0	59.93717632056407	0.0
36	0.007444702073829831	0.0	0.0	60.35996335285366	0.0
37	0.007444702073829831	0.0	0.0	60.79932085104952	0.0
38	0.007444702073829831	0.0	0.0	61.21010029934907	0.0
39	0.007564777913730312	0.0	0.0	61.62508240204513	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGCA	65	0.0	45.000004	16
CGCGGCT	30	2.1647793E-6	45.000004	3
GCTATCA	35	1.2113924E-7	45.000004	42
TTGTAGG	155	0.0	45.000004	1
TTGTAGC	30	2.1647793E-6	45.000004	1
CTTGCGG	120	0.0	45.000004	1
ATGCGGC	35	1.2113924E-7	45.000004	2
CGATGTA	30	2.1647793E-6	45.000004	9
TAAGGGT	95	0.0	45.000004	3
TGGGCGC	35	1.2113924E-7	45.000004	5
AGCTATC	35	1.2113924E-7	45.000004	41
TATACGG	65	0.0	45.000004	1
GCGTAGG	120	0.0	45.000004	1
ACCCACG	35	1.2113924E-7	45.000004	22
TGCACGG	65	0.0	45.000004	1
GAGATGT	30	2.1647793E-6	45.000004	8
AGCACGG	60	0.0	45.000004	1
TCACGGC	20	7.0321665E-4	45.0	2
AGCGTGT	20	7.0321665E-4	45.0	24
GGTACCC	20	7.0321665E-4	45.0	28
>>END_MODULE