FastQCFastQC Report
Thu 26 May 2016
SRR1043318_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043318_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences626732
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353865.646113490295693No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT43480.6937574593287082Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCTT41170.656899599828954Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT35490.5662707504962249Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT14680.2342308993317718Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC14610.23311399449844591Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC13490.2152435171652317Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG13280.21189280266525404No Hit
GCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC11210.17886433116547423Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC9590.15301596216564656Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT8770.13993221983240045Illumina Single End Adapter 2 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC8310.13259255949911605Illumina Single End Adapter 2 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG8090.1290822871658061No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCA7820.12477422566583485No Hit
GGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC7080.11296694599924688Illumina Single End Adapter 2 (95% over 21bp)
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTG6720.10722286399928518No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT6690.10674419049928838No Hit
ATAGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT6530.10419126516597206No Hit
TGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC6520.10403170733263979Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC206.3092477E-446.020
GTTGATA301.8600385E-646.012
AACCGTT206.3092477E-446.012
CGGGTCC206.3092477E-446.05
CGTGGGC253.4153643E-546.03
AAGTAGC253.4153643E-546.017
TCGTTCA206.3092477E-446.015
GGTACAT301.8600385E-646.07
TCGCAGG600.046.01
ACCGGGT253.4153643E-546.03
GATGTTC551.8189894E-1246.08
TCCGCGG301.8600385E-646.01
CTACTCG206.3092477E-446.042
CGACGGT900.046.027
ACGTTGG206.3092477E-446.01
GGCACGG750.046.01
TGGGTTG253.4153643E-546.05
GGCGTTA206.3092477E-446.046
GTCCTAA600.046.018
GTGGGCG206.3092477E-446.04
Produced by FastQC (version 0.11.5)