##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043318_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	626732
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24775023455002	33.0	31.0	34.0	31.0	34.0
2	32.57863967373614	34.0	31.0	34.0	31.0	34.0
3	32.669989724475535	34.0	31.0	34.0	31.0	34.0
4	36.17113534971886	37.0	35.0	37.0	35.0	37.0
5	36.06600748007123	37.0	35.0	37.0	35.0	37.0
6	36.30420498713964	37.0	37.0	37.0	35.0	37.0
7	36.228845822456805	37.0	37.0	37.0	35.0	37.0
8	36.209799403891935	37.0	37.0	37.0	35.0	37.0
9	37.776596695238155	39.0	38.0	39.0	35.0	39.0
10	37.62867222353414	39.0	37.0	39.0	35.0	39.0
11	37.22053126376186	39.0	37.0	39.0	35.0	39.0
12	37.14673417026736	39.0	37.0	39.0	34.0	39.0
13	37.16143582903059	39.0	37.0	39.0	34.0	39.0
14	38.552895017327984	40.0	37.0	41.0	35.0	41.0
15	38.678599465162144	40.0	38.0	41.0	35.0	41.0
16	38.69599605573036	40.0	37.0	41.0	35.0	41.0
17	38.71468346917023	40.0	37.0	41.0	35.0	41.0
18	38.47572806239349	40.0	37.0	41.0	35.0	41.0
19	38.21605087980189	40.0	37.0	41.0	35.0	41.0
20	37.94135292278039	40.0	35.0	41.0	34.0	41.0
21	37.89851324010901	40.0	35.0	41.0	34.0	41.0
22	37.84812647192101	40.0	35.0	41.0	34.0	41.0
23	37.850409744516	40.0	35.0	41.0	34.0	41.0
24	37.749763854406666	40.0	35.0	41.0	34.0	41.0
25	37.65630604468896	39.0	35.0	41.0	34.0	41.0
26	37.60140378981766	39.0	35.0	41.0	34.0	41.0
27	37.64490882865403	39.0	35.0	41.0	34.0	41.0
28	37.62889241334414	40.0	35.0	41.0	34.0	41.0
29	37.568142363881215	40.0	35.0	41.0	34.0	41.0
30	37.484213347970105	40.0	35.0	41.0	34.0	41.0
31	37.350256568995995	40.0	35.0	41.0	33.0	41.0
32	37.1795504298488	40.0	35.0	41.0	33.0	41.0
33	36.99282627981338	40.0	35.0	41.0	33.0	41.0
34	36.834466725809435	40.0	35.0	41.0	33.0	41.0
35	36.67396271452551	39.0	35.0	41.0	32.0	41.0
36	36.61254730889758	39.0	35.0	41.0	32.0	41.0
37	36.528281625958144	39.0	35.0	41.0	31.0	41.0
38	36.479862205855135	39.0	35.0	41.0	31.0	41.0
39	36.40071673378733	39.0	35.0	41.0	31.0	41.0
40	36.35458856417097	39.0	35.0	41.0	31.0	41.0
41	36.3557102557393	39.0	35.0	41.0	31.0	41.0
42	36.205264770268634	39.0	35.0	41.0	31.0	41.0
43	36.01314756546658	39.0	35.0	41.0	30.0	41.0
44	35.83139683309612	38.0	35.0	41.0	30.0	41.0
45	35.75248750662165	38.0	35.0	41.0	29.0	41.0
46	35.717486900301886	38.0	35.0	41.0	29.0	41.0
47	35.625152377730835	38.0	35.0	40.0	28.0	41.0
48	35.66555242113056	38.0	35.0	41.0	29.0	41.0
49	35.54830932519801	38.0	35.0	40.0	28.0	41.0
50	35.38689742984242	38.0	35.0	40.0	27.0	41.0
51	35.25131316096832	38.0	35.0	40.0	27.0	41.0
52	34.91075451708226	37.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	5.0
16	13.0
17	40.0
18	65.0
19	204.0
20	344.0
21	598.0
22	948.0
23	1661.0
24	3214.0
25	5727.0
26	8103.0
27	8510.0
28	8191.0
29	8323.0
30	9245.0
31	11145.0
32	14028.0
33	18449.0
34	35714.0
35	75002.0
36	58139.0
37	45397.0
38	76073.0
39	236868.0
40	723.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.01213277764659	19.79745728636802	24.78332046233478	27.40708947365062
2	31.859550812787603	20.88324834219411	26.99239866482005	20.264802180198235
3	21.9052162646873	22.167210227018884	36.79419592425471	19.13337758403911
4	23.260181385344932	24.89166023116739	33.801210086607995	18.046948296879687
5	19.217145446538552	30.394171671464036	31.066707938959553	19.321974943037855
6	74.16710811000556	1.654774289488968	20.319370959197872	3.858746641307608
7	74.47904367417014	1.54100955432306	16.414512104057234	7.565434667449564
8	62.36956147125087	3.264553269978236	25.001595578333323	9.36428968043757
9	33.72079293860853	20.43090188469713	28.078827951979473	17.76947722471487
10	27.4796882878168	18.38856161804408	42.09662184155269	12.035128252586432
11	25.644134973162373	15.745166993228366	38.15410733774564	20.45659069586362
12	16.31686271005789	24.356503258170957	41.74192477805506	17.584709253716102
13	11.755582928588296	27.92182304398052	40.71038338556193	19.61221064186925
14	9.507891730436615	22.53578882201643	47.40495139868396	20.55136804886299
15	10.104797584932633	17.71187684688192	50.545368674329694	21.637956893855748
16	10.160642826598929	24.573821027169508	38.37557361041083	26.889962535820732
17	12.9200359962472	21.743424621688376	42.454988735216965	22.881550646847458
18	15.748039034228348	28.405602394643964	38.455512084910296	17.390846486217395
19	16.854891724054298	22.7843799263481	35.592725439262715	24.76800291033488
20	12.26696578441822	30.983737865626775	34.86418437226757	21.885111977687433
21	14.14687617673902	23.666575186842223	34.67080027826886	27.515748358149892
22	10.820574025261196	28.155894385478963	31.581760624956125	29.44177096430372
23	10.768239055928213	22.494622901016704	45.680609893862126	21.056528149192957
24	15.866909620060888	22.105142229852632	39.3445683322377	22.683379817848778
25	11.39657780359069	28.005750464313294	37.40865314041727	23.18901859167874
26	13.200219551578666	26.91086461198726	34.95624924210029	24.93266659433378
27	18.95882131437361	25.514893128163234	37.74372459041504	17.782560967048116
28	14.214847813738569	25.083289188999448	34.10772068443928	26.594142312822704
29	16.401907035223985	30.65249580362898	30.93188156979379	22.013715591353243
30	20.953772904526975	29.25891768730494	28.77226629564152	21.015043112526566
31	25.4296892451638	25.06190843933292	28.797955106808015	20.710447208695264
32	18.48381764454344	34.161491674272256	27.104248705985974	20.25044197519833
33	17.69687841054869	27.300026167484663	33.27355233177818	21.729543090188468
34	23.16939297817887	26.389110496990742	29.659248291135604	20.782248233694787
35	26.21997919365853	25.989737240160064	31.02203174562652	16.768251820554877
36	20.38622569136409	26.16190014232559	32.05421136945297	21.39766279685735
37	24.71104076383526	23.361819725177586	35.62734948909581	16.299790021891337
38	23.19875161951201	22.47675242368349	29.362470721137583	24.96202523566692
39	26.17594123165883	19.572480741369517	32.37109322645086	21.880484800520797
40	16.687196441222085	18.900263589540664	37.887486198247416	26.52505377098983
41	21.607321789855952	23.834749143174434	28.350874057810994	26.20705500915862
42	21.253262957691646	19.704913743035302	34.77882093143481	24.263002367838247
43	23.45611840467696	23.301187748511325	32.930502990113794	20.312190856697917
44	16.046571740393023	27.959638250480268	33.17893453661214	22.814855472514566
45	21.26826139402488	22.967392761180218	33.927260774940486	21.83708506985442
46	25.625466706662497	20.014615497533235	31.442466636457052	22.917451159347216
47	18.79862524970801	18.368776446710875	37.1650083289189	25.667589974662214
48	19.380213552204133	15.859091286227606	32.708558043948614	32.05213711761965
49	21.110458696859265	17.892177198547383	37.53917144808307	23.458192656510278
50	19.33298443353778	17.340745326551062	37.29329282691805	26.032977412993112
51	17.26208331471825	15.529444802563136	37.080921350752796	30.12755053196582
52	16.880420977387463	15.711021616895259	44.16832074953888	23.240236656178396
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	220.0
1	1021.0
2	1822.0
3	20193.0
4	38564.0
5	21102.5
6	3641.0
7	3561.5
8	3482.0
9	3704.0
10	3926.0
11	3985.5
12	4045.0
13	3963.5
14	3628.5
15	3375.0
16	3091.5
17	2808.0
18	2503.5
19	2199.0
20	2095.5
21	1992.0
22	1839.0
23	1686.0
24	1710.5
25	1735.0
26	2390.5
27	3046.0
28	2782.0
29	2518.0
30	2886.5
31	3255.0
32	4743.5
33	6232.0
34	7705.5
35	9179.0
36	10343.5
37	11508.0
38	12369.5
39	14558.5
40	15886.0
41	22234.0
42	28582.0
43	37672.5
44	46763.0
45	57585.0
46	68407.0
47	81758.0
48	95109.0
49	94633.5
50	94158.0
51	78422.5
52	62687.0
53	48228.5
54	33770.0
55	26094.5
56	18419.0
57	15403.0
58	12387.0
59	10471.5
60	8556.0
61	7464.0
62	6372.0
63	5195.5
64	3462.5
65	2906.0
66	2354.0
67	1802.0
68	1431.5
69	1061.0
70	1161.5
71	1262.0
72	1160.5
73	1059.0
74	804.5
75	550.0
76	416.5
77	283.0
78	222.5
79	162.0
80	124.5
81	87.0
82	59.5
83	32.0
84	46.5
85	61.0
86	33.0
87	5.0
88	3.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	626732.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.651477916605987
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.3348989578336	20.725017600902948
2	11.73209098130734	6.722834913330791
3	4.700691619693679	4.040452864032849
4	2.4520979102575993	2.810249165004052
5	1.5165349104630859	2.1725483248447572
6	0.963486136509329	1.6563181057891836
7	0.6265588899053138	1.2566286738302683
8	0.47696604129421405	1.0932625599289723
9	0.3864952893316115	0.9966295122441217
>10	3.960518844721831	25.993848454862302
>50	0.6348680547758704	12.167525983049076
>100	0.20024913093370983	9.86101358943399
>500	0.009509036943779518	1.8290874060817506
>1k	0.004474840914719774	3.0034983858212643
>5k	0.0	0.0
>10k+	5.593551143399717E-4	5.671084460843672
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35386	5.646113490295693	No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	4348	0.6937574593287082	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCTT	4117	0.656899599828954	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	3549	0.5662707504962249	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	1468	0.2342308993317718	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	1461	0.23311399449844591	Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	1349	0.2152435171652317	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG	1328	0.21189280266525404	No Hit
GCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	1121	0.17886433116547423	Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	959	0.15301596216564656	Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	877	0.13993221983240045	Illumina Single End Adapter 2 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	831	0.13259255949911605	Illumina Single End Adapter 2 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG	809	0.1290822871658061	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCA	782	0.12477422566583485	No Hit
GGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	708	0.11296694599924688	Illumina Single End Adapter 2 (95% over 21bp)
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTG	672	0.10722286399928518	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	669	0.10674419049928838	No Hit
ATAGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	653	0.10419126516597206	No Hit
TGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	652	0.10403170733263979	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7025331401619831	0.0
2	0.0	0.0	0.0	2.4380436933170797	0.0
3	0.0	0.0	0.0	4.014953760139901	0.0
4	0.0	0.0	0.0	5.064206072132905	0.0
5	0.0	0.0	0.0	10.352271784430984	0.0
6	0.0	0.0	0.0	11.488642673423408	0.0
7	0.0	0.0	0.0	15.874727953894169	0.0
8	0.0	0.0	0.0	21.159762067358933	0.0
9	0.0	0.0	0.0	25.132752117332448	0.0
10	0.0	0.0	0.0	27.44314954398371	0.0
11	0.0	0.0	0.0	29.50846613863661	0.0
12	0.0	0.0	0.0	31.004001710459974	0.0
13	0.0	0.0	0.0	31.64127569678906	0.0
14	0.0	0.0	0.0	32.055328274286296	0.0
15	0.0	0.0	0.0	33.538099219443076	0.0
16	0.0	0.0	0.0	35.17723684126548	0.0
17	3.1911566666453924E-4	0.0	0.0	37.29536707875136	0.0
18	3.1911566666453924E-4	0.0	0.0	38.17133958374552	0.0
19	3.1911566666453924E-4	0.0	0.0	39.17559658673883	0.0
20	3.1911566666453924E-4	0.0	0.0	40.21990260589853	0.0
21	0.003031598833313123	0.0	0.0	41.20102372305866	0.0
22	0.0035102723333099313	0.0	0.0	42.21277994421858	0.0
23	0.003829387999974471	0.0	0.0	42.92233362904719	0.0
24	0.005903639833293976	0.0	0.0	43.47408461671017	0.0
25	0.006063197666626246	0.0	0.0	43.9944027112067	0.0
26	0.006063197666626246	0.0	0.0	44.52253913953652	0.0
27	0.006063197666626246	0.0	0.0	45.03009260736646	0.0
28	0.006382313333290784	0.0	0.0	45.56189886586292	0.0
29	0.006382313333290784	0.0	0.0	46.22837193569181	0.0
30	0.006382313333290784	0.0	0.0	46.81777857202121	0.0
31	0.006382313333290784	0.0	0.0	47.27044414518486	0.0
32	0.006382313333290784	0.0	0.0	47.74816029818168	0.0
33	0.006382313333290784	0.0	0.0	48.20848464734528	0.0
34	0.006382313333290784	0.0	0.0	48.815123529674565	0.0
35	0.006382313333290784	0.0	0.0	49.23428195783844	0.0
36	0.006382313333290784	0.0	0.0	49.65918446800227	0.0
37	0.006382313333290784	0.0	0.0	50.10100010849933	0.0
38	0.006382313333290784	0.0	0.0	50.51983942099653	0.0
39	0.006382313333290784	0.0	0.0	50.93437067199377	0.0
40	0.006382313333290784	0.0	0.0	51.39629059949069	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	20	6.3092477E-4	46.0	20
GTTGATA	30	1.8600385E-6	46.0	12
AACCGTT	20	6.3092477E-4	46.0	12
CGGGTCC	20	6.3092477E-4	46.0	5
CGTGGGC	25	3.4153643E-5	46.0	3
AAGTAGC	25	3.4153643E-5	46.0	17
TCGTTCA	20	6.3092477E-4	46.0	15
GGTACAT	30	1.8600385E-6	46.0	7
TCGCAGG	60	0.0	46.0	1
ACCGGGT	25	3.4153643E-5	46.0	3
GATGTTC	55	1.8189894E-12	46.0	8
TCCGCGG	30	1.8600385E-6	46.0	1
CTACTCG	20	6.3092477E-4	46.0	42
CGACGGT	90	0.0	46.0	27
ACGTTGG	20	6.3092477E-4	46.0	1
GGCACGG	75	0.0	46.0	1
TGGGTTG	25	3.4153643E-5	46.0	5
GGCGTTA	20	6.3092477E-4	46.0	46
GTCCTAA	60	0.0	46.0	18
GTGGGCG	20	6.3092477E-4	46.0	4
>>END_MODULE