FastQCFastQC Report
Thu 26 May 2016
SRR1043317_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043317_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences609681
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326885.361492321394303No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC42730.7008583177104092Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT39390.6460755706672834Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC36790.6034303184780238Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC14830.24324195767950782Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG14290.23438486684020005No Hit
ACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG14070.23077642242418575No Hit
GCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG12320.20207288729679945No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCC12240.2007607256909761No Hit
AGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG10230.16779266534466386No Hit
ACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC8700.14269757463329186Illumina Single End Adapter 2 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT7820.12826379696923473No Hit
TGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCTT7610.12481937275394839Illumina Single End Adapter 2 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG7360.12071886773575033No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC7050.11563424151318476No Hit
ATAGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC6920.11350197890372178No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT6790.1113697162942588No Hit
GGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG6790.1113697162942588No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC6720.11022157488916336No Hit
TGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG6390.10480890826514194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCAGG302.163817E-645.0000041
GGCACTT302.163817E-645.0000047
GCAACAA453.8380676E-1045.0000048
TGGGTAC700.045.0000045
ATTACGG453.8380676E-1045.0000041
GACCGAG302.163817E-645.00000419
TTCACGG600.045.0000041
ACACAGG453.8380676E-1045.0000041
CACGACC302.163817E-645.00000426
TAACCGG600.045.0000041
ATGACGG351.2106648E-745.0000041
GCGATGA302.163817E-645.0000048
TTAGTGC302.163817E-645.00000426
TAGTGCA302.163817E-645.00000427
GCTAAGG1450.045.0000041
CGATCTC453.8380676E-1045.0000049
AATACGG453.8380676E-1045.0000041
CCCATGG302.163817E-645.0000041
TGCACGG700.045.0000041
ACGACCA302.163817E-645.00000427
Produced by FastQC (version 0.11.5)