##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043317_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	609681
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.218878397063385	33.0	31.0	34.0	30.0	34.0
2	32.496123382555794	34.0	31.0	34.0	31.0	34.0
3	32.57724777383583	34.0	31.0	34.0	31.0	34.0
4	36.12827199797927	37.0	35.0	37.0	35.0	37.0
5	36.08002545593515	37.0	35.0	37.0	35.0	37.0
6	36.30778062626193	37.0	37.0	37.0	35.0	37.0
7	36.23924478538777	37.0	37.0	37.0	35.0	37.0
8	36.144263967550245	37.0	37.0	37.0	35.0	37.0
9	37.713404222864085	39.0	38.0	39.0	35.0	39.0
10	37.56688661775584	39.0	37.0	39.0	35.0	39.0
11	37.202432091536394	39.0	37.0	39.0	35.0	39.0
12	37.135688991456185	39.0	37.0	39.0	34.0	39.0
13	37.16639685343647	39.0	37.0	39.0	34.0	39.0
14	38.54902645809858	40.0	37.0	41.0	35.0	41.0
15	38.68677062266989	40.0	38.0	41.0	35.0	41.0
16	38.72851868436116	40.0	38.0	41.0	35.0	41.0
17	38.73188929948613	40.0	37.0	41.0	35.0	41.0
18	38.51815293571556	40.0	37.0	41.0	35.0	41.0
19	38.282036015555676	40.0	37.0	41.0	35.0	41.0
20	38.03676676819517	40.0	35.0	41.0	35.0	41.0
21	37.99224348470758	40.0	35.0	41.0	34.0	41.0
22	37.96076144737986	40.0	35.0	41.0	34.0	41.0
23	37.92607445532992	40.0	35.0	41.0	34.0	41.0
24	37.82528404198261	40.0	35.0	41.0	34.0	41.0
25	37.79381676647296	40.0	35.0	41.0	34.0	41.0
26	37.749122901976605	40.0	35.0	41.0	34.0	41.0
27	37.7626627695467	40.0	35.0	41.0	34.0	41.0
28	37.64514885653317	40.0	35.0	41.0	34.0	41.0
29	37.67648655608425	40.0	35.0	41.0	34.0	41.0
30	37.53766313859215	40.0	35.0	41.0	34.0	41.0
31	37.41022600343458	40.0	35.0	41.0	34.0	41.0
32	37.242749897077324	40.0	35.0	41.0	33.0	41.0
33	37.042069541284704	40.0	35.0	41.0	33.0	41.0
34	36.843191767498084	40.0	35.0	41.0	33.0	41.0
35	36.68924404729687	39.0	35.0	41.0	32.0	41.0
36	36.63047560937605	39.0	35.0	41.0	32.0	41.0
37	36.51244175232622	39.0	35.0	41.0	31.0	41.0
38	36.46287976827226	39.0	35.0	41.0	31.0	41.0
39	36.34914816108752	39.0	35.0	41.0	31.0	41.0
40	36.276208377823814	39.0	35.0	41.0	31.0	41.0
41	36.237110882576296	39.0	35.0	41.0	31.0	41.0
42	36.090381691409114	39.0	35.0	41.0	30.0	41.0
43	35.90639039104056	38.0	35.0	41.0	30.0	41.0
44	35.80490781244618	38.0	35.0	40.0	30.0	41.0
45	35.75576079950007	38.0	35.0	40.0	29.0	41.0
46	35.66435890244242	38.0	35.0	40.0	29.0	41.0
47	35.5254173904058	38.0	35.0	40.0	28.0	41.0
48	35.57034908419321	38.0	35.0	40.0	28.0	41.0
49	35.470382052253555	38.0	35.0	40.0	28.0	41.0
50	35.32422529158691	38.0	35.0	40.0	27.0	41.0
51	34.837790582288115	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	7.0
16	12.0
17	20.0
18	68.0
19	155.0
20	301.0
21	501.0
22	891.0
23	1485.0
24	2681.0
25	4708.0
26	7161.0
27	8469.0
28	8399.0
29	8350.0
30	9249.0
31	10867.0
32	13296.0
33	18152.0
34	35133.0
35	72858.0
36	54532.0
37	44432.0
38	78733.0
39	229075.0
40	144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.491457007845085	19.785592793608462	24.880224248418433	26.842725950128017
2	31.529275145526924	20.864189633595274	27.104174150088323	20.50236107078948
3	21.995108917614292	22.32741384428906	36.45070126836821	19.226775969728433
4	23.43061371438506	25.019477398836443	33.45749662528437	18.092412261494125
5	19.434589564050707	30.572873355082415	30.622243435501517	19.370293645365365
6	74.5542340994717	1.6073979671336323	19.855465399118554	3.9829025342761213
7	74.94837464182089	1.4932399074269986	15.989837308362898	7.568548142389217
8	62.680155687974526	3.170674500271453	24.566617624626648	9.582552187127366
9	33.9799009646028	20.45069470756018	27.628874772216943	17.94052955562007
10	27.806672669806016	18.380431733972355	41.77676522640528	12.036130369816346
11	25.87664696784056	15.782351754442079	37.77942891446511	20.56157236325226
12	16.41891415346714	24.327148131563884	41.51006838002168	17.74386933494729
13	11.890152391168495	27.902788507432575	40.50577269096462	19.70128641043431
14	9.553192571197068	22.47913252996239	47.164336759715326	20.803338139125216
15	10.164987919912216	17.61560553797806	50.45015344089778	21.769253101211945
16	10.143009213014675	24.409978332931484	38.254759456174625	27.19225299787922
17	13.065193109183326	21.715618495573917	42.2509476267097	22.96824076853305
18	15.958509450023865	28.291024322555565	38.27509796106489	17.475368266355684
19	16.86406497824272	22.819966507075012	35.33634802462271	24.979620490059556
20	12.176203621237992	31.11135167407218	34.555939909559264	22.15650479513057
21	14.183646857947025	23.739463752355743	34.431284557006045	27.645604832691195
22	10.945888095577851	28.21918347463674	31.234202804417393	29.600725625368018
23	10.827137470250836	22.578200731202056	45.286469481581356	21.30819231696576
24	15.908975349404034	22.08942053303285	39.06403512656619	22.937568990996933
25	11.402192294002928	28.17539008104238	37.058068071663705	23.36434955329098
26	13.343863430220065	26.82927629366833	34.66238901983168	25.164471256279924
27	19.06406793060633	25.600108909413283	37.461393745253666	17.874429414726716
28	14.364889179751378	25.065075014638804	33.84852078381974	26.721515021790083
29	16.57194500074629	30.72393595995283	30.435096386470956	22.26902265282992
30	21.17238359076304	29.09341114451656	28.557721168939164	21.176484095781237
31	25.85384815993938	24.89859451089996	28.379759251149373	20.867798078011287
32	18.74619678159562	34.02976310562409	26.797784415128568	20.426255697651722
33	17.803736708212984	27.24162307829832	32.935092285965936	22.019547927522755
34	23.52082482478542	26.303099489733157	29.52035572701134	20.655719958470083
35	26.4079083979983	25.951440179372494	30.744602505244544	16.896048917384665
36	20.621111696116493	26.216496823748813	31.612105346894527	21.55028613324017
37	25.00373145956656	23.309566806247858	35.34176069124673	16.34494104293885
38	23.529681915624728	22.498650933849014	28.971544135375716	25.000123015150542
39	26.422506195863082	19.538414351111484	31.982791000539628	22.05628845248581
40	16.91851968488439	18.70765203442456	37.65969416793372	26.714134112757325
41	21.867829241849428	23.695670358761383	27.978893880570332	26.45760651881886
42	21.51239746687202	19.5126631795972	34.5315008996508	24.443438453879978
43	23.713712580841456	23.130456747052968	32.73187125726405	20.423959414841534
44	16.26178280116979	27.692022549497196	33.18407495067092	22.862119698662088
45	21.418085851453466	22.68202551826283	33.74321981495241	22.156668815331297
46	25.943239169336096	19.77378333915605	31.202546905676904	23.080430585830953
47	18.944661224476405	18.16064466499694	37.20880263613267	25.685891474393983
48	19.528573139067806	15.707722563110874	32.414656189056245	32.349048108765075
49	21.163198459522274	17.805376910220264	37.28900851428862	23.742416115968844
50	19.595001320362616	17.224417359241965	37.240458534873156	25.940122785522263
51	17.453225539257414	15.431020484482868	36.6926310644419	30.423122911817817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	190.0
1	957.0
2	1724.0
3	18656.0
4	35588.0
5	19647.5
6	3707.0
7	3605.5
8	3504.0
9	3659.0
10	3814.0
11	3836.0
12	3858.0
13	3674.5
14	3491.0
15	3224.0
16	2957.0
17	2665.5
18	2374.0
19	2168.0
20	1962.0
21	1814.5
22	1667.0
23	1562.0
24	1457.0
25	1490.0
26	2159.5
27	2796.0
28	2686.5
29	2577.0
30	3507.0
31	4437.0
32	5298.5
33	6160.0
34	8371.5
35	10583.0
36	11053.0
37	11523.0
38	12540.0
39	13557.0
40	17836.5
41	22116.0
42	28763.5
43	35411.0
44	41774.5
45	48138.0
46	60921.5
47	73705.0
48	87587.0
49	101469.0
50	95794.5
51	90120.0
52	69366.0
53	48612.0
54	36999.5
55	25387.0
56	19572.5
57	13758.0
58	11804.5
59	9851.0
60	8208.0
61	6565.0
62	5593.5
63	4622.0
64	4048.0
65	3474.0
66	2800.5
67	2127.0
68	1657.5
69	1188.0
70	1127.5
71	1067.0
72	1184.5
73	1302.0
74	927.5
75	465.5
76	378.0
77	288.5
78	199.0
79	136.0
80	73.0
81	56.0
82	39.0
83	47.0
84	55.0
85	36.0
86	17.0
87	10.5
88	4.0
89	2.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	609681.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.276290446160708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.84302656566621	20.314542857020175
2	11.691882617786044	6.61206137525872
3	4.856225082004943	4.119480926721071
4	2.499613429809035	2.827191813776169
5	1.5577008466972546	2.202300078472101
6	0.9755936835623477	1.6551702212309247
7	0.6858061937280019	1.3574438586540258
8	0.5116697247865527	1.1574497400457342
9	0.3946639617381154	1.0043669529665467
>10	4.137512317538331	26.7311406684885
>50	0.6366326379617167	12.043358208133165
>100	0.19503097372700237	9.662078159801892
>500	0.008785178996711818	1.63935539317277
>1k	0.005271107398027091	3.2606594945124425
>5k	0.0	0.0
>10k+	5.856785997807879E-4	5.4134002517457835
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32688	5.361492321394303	No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	4273	0.7008583177104092	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	3939	0.6460755706672834	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	3679	0.6034303184780238	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	1483	0.24324195767950782	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG	1429	0.23438486684020005	No Hit
ACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG	1407	0.23077642242418575	No Hit
GCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG	1232	0.20207288729679945	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCC	1224	0.2007607256909761	No Hit
AGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG	1023	0.16779266534466386	No Hit
ACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	870	0.14269757463329186	Illumina Single End Adapter 2 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	782	0.12826379696923473	No Hit
TGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCTT	761	0.12481937275394839	Illumina Single End Adapter 2 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG	736	0.12071886773575033	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	705	0.11563424151318476	No Hit
ATAGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	692	0.11350197890372178	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	679	0.1113697162942588	No Hit
GGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG	679	0.1113697162942588	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	672	0.11022157488916336	No Hit
TGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG	639	0.10480890826514194	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6851123784405287	0.0
2	0.0	0.0	0.0	2.4839219198236453	0.0
3	0.0	0.0	0.0	4.101489139402409	0.0
4	0.0	0.0	0.0	5.165488181524436	0.0
5	0.0	0.0	0.0	10.507298078831388	0.0
6	0.0	0.0	0.0	11.66806903938289	0.0
7	0.0	0.0	0.0	16.0867732469931	0.0
8	0.0	0.0	0.0	21.466963871270387	0.0
9	0.0	0.0	0.0	25.514490364633307	0.0
10	0.0	0.0	0.0	27.904264689239128	0.0
11	0.0	0.0	0.0	30.0206173392315	0.0
12	0.0	0.0	0.0	31.60144403384721	0.0
13	0.0	0.0	0.0	32.245715382306486	0.0
14	0.0	0.0	0.0	32.69267042929007	0.0
15	0.0	0.0	0.0	34.2324920737238	0.0
16	0.0	0.0	0.0	35.92550858563741	0.0
17	3.280404014558433E-4	0.0	0.0	38.03530042760066	0.0
18	3.280404014558433E-4	0.0	0.0	38.93609936999841	0.0
19	3.280404014558433E-4	0.0	0.0	39.960897584146466	0.0
20	3.280404014558433E-4	0.0	0.0	41.017187676834276	0.0
21	0.00295236361310259	0.0	0.0	41.98621902273484	0.0
22	0.0034444242152863545	0.0	0.0	43.02151452972948	0.0
23	0.003772464616742198	0.0	0.0	43.74894411995781	0.0
24	0.00492060602183765	0.0	0.0	44.32088255989608	0.0
25	0.00492060602183765	0.0	0.0	44.856900575874924	0.0
26	0.00492060602183765	0.0	0.0	45.39636301606906	0.0
27	0.00492060602183765	0.0	0.0	45.93402123405519	0.0
28	0.005084626222565571	0.0	0.0	46.48906559331847	0.0
29	0.005084626222565571	0.0	0.0	47.17598219396701	0.0
30	0.005084626222565571	0.0	0.0	47.75366134093075	0.0
31	0.005084626222565571	0.0	0.0	48.21078564035947	0.0
32	0.005084626222565571	0.0	0.0	48.70858694956871	0.0
33	0.005084626222565571	0.0	0.0	49.17046783481854	0.0
34	0.005084626222565571	0.0	0.0	49.78406740574169	0.0
35	0.005084626222565571	0.0	0.0	50.21051992763429	0.0
36	0.005084626222565571	0.0	0.0	50.631231742501406	0.0
37	0.005084626222565571	0.0	0.0	51.08327141570756	0.0
38	0.005084626222565571	0.0	0.0	51.512676301213254	0.0
39	0.005084626222565571	0.0	0.0	51.9488060149488	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCAGG	30	2.163817E-6	45.000004	1
GGCACTT	30	2.163817E-6	45.000004	7
GCAACAA	45	3.8380676E-10	45.000004	8
TGGGTAC	70	0.0	45.000004	5
ATTACGG	45	3.8380676E-10	45.000004	1
GACCGAG	30	2.163817E-6	45.000004	19
TTCACGG	60	0.0	45.000004	1
ACACAGG	45	3.8380676E-10	45.000004	1
CACGACC	30	2.163817E-6	45.000004	26
TAACCGG	60	0.0	45.000004	1
ATGACGG	35	1.2106648E-7	45.000004	1
GCGATGA	30	2.163817E-6	45.000004	8
TTAGTGC	30	2.163817E-6	45.000004	26
TAGTGCA	30	2.163817E-6	45.000004	27
GCTAAGG	145	0.0	45.000004	1
CGATCTC	45	3.8380676E-10	45.000004	9
AATACGG	45	3.8380676E-10	45.000004	1
CCCATGG	30	2.163817E-6	45.000004	1
TGCACGG	70	0.0	45.000004	1
ACGACCA	30	2.163817E-6	45.000004	27
>>END_MODULE