##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043316_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2552238
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.299226404434066	33.0	31.0	34.0	31.0	34.0
2	32.646979239396956	34.0	31.0	34.0	31.0	34.0
3	32.77502960147134	34.0	31.0	34.0	31.0	34.0
4	36.22733734079659	37.0	37.0	37.0	35.0	37.0
5	36.17422473922886	37.0	37.0	37.0	35.0	37.0
6	36.276359806569765	37.0	37.0	37.0	35.0	37.0
7	36.207702808280416	37.0	37.0	37.0	35.0	37.0
8	36.212075049427206	37.0	37.0	37.0	35.0	37.0
9	37.798107386536834	39.0	38.0	39.0	35.0	39.0
10	37.69487093288322	39.0	37.0	39.0	35.0	39.0
11	37.484483422000615	39.0	37.0	39.0	35.0	39.0
12	37.50339858586856	39.0	37.0	39.0	35.0	39.0
13	37.51752932132505	39.0	37.0	39.0	35.0	39.0
14	38.924903163419714	41.0	38.0	41.0	35.0	41.0
15	38.96457187770106	41.0	38.0	41.0	35.0	41.0
16	38.88347364156478	41.0	38.0	41.0	35.0	41.0
17	38.86645328531274	41.0	38.0	41.0	35.0	41.0
18	38.57804170300732	40.0	38.0	41.0	35.0	41.0
19	38.267073838725075	40.0	37.0	41.0	35.0	41.0
20	37.949885159612855	40.0	35.0	41.0	34.0	41.0
21	37.89825517839637	40.0	35.0	41.0	34.0	41.0
22	37.829526478329996	40.0	35.0	41.0	34.0	41.0
23	37.83858637007991	40.0	35.0	41.0	34.0	41.0
24	37.72867263946387	40.0	35.0	41.0	34.0	41.0
25	37.632174193786	39.0	35.0	41.0	34.0	41.0
26	37.541981194543766	39.0	35.0	41.0	34.0	41.0
27	37.56740045403289	39.0	35.0	41.0	34.0	41.0
28	37.515580051703644	39.0	35.0	41.0	34.0	41.0
29	37.428583854640515	39.0	35.0	41.0	33.0	41.0
30	37.3421800788171	39.0	35.0	41.0	33.0	41.0
31	37.1747834645515	39.0	35.0	41.0	33.0	41.0
32	36.876472727073256	39.0	35.0	41.0	33.0	41.0
33	36.49744773018817	39.0	35.0	41.0	31.0	41.0
34	36.13045295932432	39.0	35.0	41.0	30.0	41.0
35	35.91037983134802	39.0	35.0	41.0	29.0	41.0
36	35.79467784744213	39.0	35.0	41.0	29.0	41.0
37	35.66029461202286	39.0	35.0	41.0	28.0	41.0
38	35.518982947515084	38.0	35.0	41.0	27.0	41.0
39	35.49105961121181	38.0	35.0	41.0	27.0	41.0
40	35.42744367884186	38.0	35.0	41.0	26.0	41.0
41	35.37068369015742	38.0	35.0	41.0	26.0	41.0
42	35.297802164218226	38.0	35.0	41.0	26.0	41.0
43	35.18872299526925	38.0	35.0	40.0	25.0	41.0
44	34.9986360989845	38.0	35.0	40.0	24.0	41.0
45	34.92269294634748	37.0	35.0	40.0	23.0	41.0
46	34.88928148550409	37.0	35.0	40.0	23.0	41.0
47	34.81199363068805	37.0	35.0	40.0	23.0	41.0
48	34.71321091528298	37.0	35.0	40.0	23.0	41.0
49	34.66692839774347	37.0	35.0	40.0	24.0	41.0
50	34.57435905272157	36.0	35.0	40.0	24.0	41.0
51	34.44793902449536	36.0	35.0	40.0	23.0	41.0
52	34.0805739903567	36.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	3.0
13	10.0
14	22.0
15	41.0
16	102.0
17	193.0
18	468.0
19	1017.0
20	1852.0
21	3011.0
22	4727.0
23	7885.0
24	13691.0
25	26002.0
26	40875.0
27	49877.0
28	51523.0
29	48517.0
30	46936.0
31	50661.0
32	58673.0
33	73331.0
34	144738.0
35	217704.0
36	252366.0
37	236025.0
38	365088.0
39	855057.0
40	1838.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.304531160495223	16.196765348686135	21.87374374960329	33.624959741215356
2	38.97775207484568	18.562571358940662	22.122270728670287	20.337405837543365
3	25.294114420363616	19.43972309792425	36.06524940072203	19.200913080990095
4	21.663771168676277	22.064243224965697	34.86226597989686	21.409719626461168
5	20.3202444286152	30.117684949444367	31.86556269438822	17.69650792755221
6	73.77047124915467	2.0270444997684387	20.94741948047165	3.255064770605249
7	72.99346691021762	1.9934661265916425	19.707292188267708	5.305774774923028
8	63.67924934900272	3.495833852485544	25.38493667126655	7.439980127245187
9	36.09867104870314	14.87365206536381	31.1259764959224	17.90170039001065
10	24.778331801344542	15.67894530212308	39.11488662107531	20.42783627545707
11	22.481641602389747	12.543109224139757	43.048532307723654	21.926716865746844
12	19.15138791915174	13.184507087505162	45.95692094546042	21.707184047882684
13	15.659785646949853	16.77061465270872	45.0671528282237	22.502446872117726
14	15.121356237153433	17.500640614237387	45.63046236283606	21.747540785773115
15	18.091181151601067	14.211723201362883	45.610205631293006	22.086890015743048
16	18.00372849240549	18.517552046478425	41.55517628058198	21.923543180534104
17	18.27137594534679	20.052988788663125	40.74733625939274	20.92829900659735
18	19.06201537630895	24.890311953665766	37.31431002908036	18.73336264094493
19	19.593039520608972	20.503652088872588	36.69352152894832	23.20978686157012
20	20.18189526211897	24.53599546750734	36.24266232224424	19.039446948129445
21	17.639890950608837	19.84313375163288	36.769494067559535	25.747481230198748
22	18.43056956286992	22.256153227089325	35.715869758227875	23.59740745181288
23	19.01969957347238	19.639077546843204	38.984765527352856	22.35645735233156
24	22.355203550766035	18.474256711168785	36.08938508085845	23.081154657206735
25	18.10849928572492	18.642305302248456	38.82541518463403	24.423780227392587
26	17.94115595802586	17.632172234721054	39.05791701244163	25.368754794811455
27	21.203077455942587	18.75220884572677	39.153362656617446	20.891351041713193
28	21.154335920082687	20.592828725220766	34.81266245546066	23.440172899235886
29	20.924028245014767	18.329756080741685	38.24850973929547	22.497705934948073
30	22.656899552471206	18.091141970302143	35.66140775272525	23.590550724501398
31	22.374911744124176	17.368560455568797	34.735710384376375	25.520817415930647
32	20.673346294507017	18.589645636496282	35.76320076732656	24.973807301670142
33	18.02437703693778	18.232155465125118	37.7706154363347	25.972852061602403
34	18.521274269876084	19.849951297645436	34.87597943451982	26.752794997958656
35	18.820580212346968	22.063381236389397	31.841662102045344	27.274376449218295
36	21.185602596623042	21.6564050844788	29.507553762619317	27.650438556278843
37	20.153175370008597	21.933416867862636	30.244044638470235	27.66936312365853
38	20.53585911658709	19.21552770548828	28.779134234346483	31.469478943578146
39	22.999657555447413	17.87576237012379	33.66084981102859	25.4637302634002
40	20.765970885160396	17.374594375602904	36.38869102332933	25.470743715907375
41	20.734273214331893	21.52687171024019	32.763598065697636	24.975257009730285
42	19.98395133996124	17.892806235155184	34.66295071227684	27.46029171260674
43	22.517296584409447	19.481216093483443	34.73492675839792	23.266560563709184
44	21.17216341109254	19.77762261983404	35.67613992112021	23.374074047953208
45	21.61636179697975	19.542378101101857	34.141447623615036	24.699812478303357
46	22.184255543566078	18.612919328056396	33.786190786282475	25.41663434209506
47	20.169474790360457	19.918126757771024	34.56221559274644	25.35018285912207
48	20.856558048269793	18.381318670124024	32.349608461279864	28.41251482032632
49	19.936267699172255	16.348396975517172	36.69524550610092	27.02008981920965
50	19.729155353066602	16.395767165914776	37.77077216153039	26.10430531948823
51	18.27936893032703	15.45851131438369	37.86374938387407	28.398370371415204
52	16.273795782368257	16.760270789793115	40.170391632755255	26.795541795083373
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	615.0
1	3665.0
2	6715.0
3	99739.0
4	192763.0
5	110635.5
6	28508.0
7	26534.0
8	24560.0
9	23717.5
10	22875.0
11	21937.0
12	20999.0
13	19661.0
14	17117.5
15	15912.0
16	14887.0
17	13862.0
18	12856.0
19	11850.0
20	11291.0
21	10732.0
22	10089.0
23	9446.0
24	9465.5
25	9485.0
26	9692.0
27	9899.0
28	10829.5
29	11760.0
30	13183.0
31	14606.0
32	17456.0
33	20306.0
34	22569.0
35	24832.0
36	26698.5
37	28565.0
38	31238.5
39	36601.0
40	39290.0
41	44520.5
42	49751.0
43	57524.5
44	65298.0
45	78040.5
46	90783.0
47	115580.0
48	140377.0
49	187596.5
50	234816.0
51	255948.5
52	277081.0
53	256942.5
54	236804.0
55	208508.0
56	180212.0
57	158811.5
58	137411.0
59	125085.0
60	112759.0
61	109795.0
62	106831.0
63	108150.5
64	90882.5
65	72295.0
66	62002.0
67	51709.0
68	43314.5
69	34920.0
70	30925.5
71	26931.0
72	25845.0
73	24759.0
74	19794.0
75	14829.0
76	11188.5
77	7548.0
78	5772.0
79	3996.0
80	3032.5
81	2069.0
82	1496.0
83	923.0
84	705.5
85	488.0
86	356.0
87	224.0
88	158.0
89	57.0
90	22.0
91	19.5
92	17.0
93	11.0
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2552238.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.075630495691197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.03699038433366	23.318217866922865
2	8.980774647091131	5.581664689961511
3	3.4001529527203145	3.169856903627096
4	1.9462192197854198	2.4191995735065643
5	1.3402303075737483	2.0824250908644184
6	1.0896202850107268	2.0316382414561844
7	0.8587867267828696	1.8681137297275996
8	0.7693532647384113	1.9126510220531645
9	0.7001714586765531	1.9582442580115518
>10	5.5178244863514925	30.10523297131533
>50	0.1954881899429313	4.256363994793182
>100	0.15315333103842946	8.634346668896615
>500	0.005932955728735548	1.2697232264386396
>1k	0.0042919254207874164	2.902263745305942
>5k	6.31165503056973E-4	1.508698569229797
>10k+	3.786993018341838E-4	6.9813594478894165
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	146115	5.724975492097524	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	18054	0.7073791707513171	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	13801	0.540741106432864	No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	9528	0.373319416135956	No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCTT	7848	0.307494833945737	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	7397	0.2898240681315771	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT	7018	0.2749743558398551	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTC	6669	0.26130008251581555	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4849	0.18999011847641167	No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	4712	0.184622280523995	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT	4707	0.18442637402938125	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4097	0.16052578168650417	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCG	3706	0.14520589380770915	No Hit
CGTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG	3686	0.14442226782925416	No Hit
GGGCCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT	3010	0.1179357097574756	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	2979	0.11672108949087037	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCT	2965	0.11617255130595186	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2792	0.10939418659231623	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3174468838721154	0.0
2	0.0	0.0	0.0	1.0044517791836027	0.0
3	0.0	0.0	0.0	1.7369069812454794	0.0
4	0.0	0.0	0.0	2.243834626708011	0.0
5	0.0	0.0	0.0	4.7119821897487615	0.0
6	0.0	0.0	0.0	5.974129371947287	0.0
7	0.0	0.0	0.0	9.012168927819427	0.0
8	0.0	0.0	0.0	12.142088629665416	0.0
9	0.0	0.0	0.0	15.532799057141222	0.0
10	0.0	0.0	0.0	17.173672674727044	0.0
11	0.0	0.0	0.0	18.399537974123103	0.0
12	0.0	0.0	0.0	19.229280341410167	0.0
13	0.0	0.0	0.0	19.662037788011933	0.0
14	0.0	0.0	0.0	19.940812729847295	0.0
15	3.918129892274937E-5	0.0	0.0	20.574295970830306	0.0
16	3.918129892274937E-5	0.0	0.0	21.2359897470377	0.0
17	3.918129892274937E-5	0.0	0.0	22.126267221160408	0.0
18	3.918129892274937E-5	0.0	0.0	22.587666197274707	0.0
19	3.918129892274937E-5	0.0	0.0	23.1522295334526	0.0
20	3.918129892274937E-5	0.0	0.0	23.736148431298336	0.0
21	1.175438967682481E-4	0.0	0.0	24.35446850959824	0.0
22	1.175438967682481E-4	0.0	0.0	24.975374553627052	0.0
23	1.175438967682481E-4	0.0	0.0	25.485201615209867	0.0
24	1.5672519569099748E-4	0.0	0.0	25.923366081062973	0.0
25	1.5672519569099748E-4	0.0	0.0	26.33915802523119	0.0
26	1.5672519569099748E-4	0.0	0.0	26.78010436330781	0.0
27	1.9590649461374683E-4	0.0	0.0	27.247380534260518	0.0
28	2.350877935364962E-4	0.0	0.0	27.698553191355977	0.0
29	2.350877935364962E-4	0.0	0.0	28.150509474429892	0.0
30	2.350877935364962E-4	0.0	0.0	28.558935334400633	0.0
31	2.7426909245924555E-4	0.0	0.0	28.974257102981774	0.0
32	2.7426909245924555E-4	0.0	0.0	29.440984735749566	0.0
33	2.7426909245924555E-4	0.0	0.0	29.881343354342345	0.0
34	2.7426909245924555E-4	0.0	0.0	30.387409011228577	0.0
35	3.1345039138199496E-4	0.0	0.0	30.82537757058707	0.0
36	3.1345039138199496E-4	0.0	0.0	31.271064845833344	0.0
37	3.1345039138199496E-4	0.0	0.0	31.790687232146844	0.0
38	3.1345039138199496E-4	0.0	0.0	32.33240787105278	0.0
39	3.1345039138199496E-4	0.0	0.0	32.919853085801556	0.0
40	3.1345039138199496E-4	0.0	0.0	33.48038074819041	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	37940	0.0	45.327095	1
TCACGAC	405	0.0	43.728397	24
CACTTAC	4475	0.0	43.173187	36
CGCACTT	4525	0.0	42.79779	34
ACTTACT	4430	0.0	42.781036	37
GCACTTA	4555	0.0	42.667397	35
CACGACG	395	0.0	42.50633	25
CCGCACT	4570	0.0	42.426697	33
ACCCGCA	4660	0.0	42.002144	31
CTTACTG	4510	0.0	41.920177	38
ATCAACG	5375	0.0	41.80651	14
TCAACGC	5385	0.0	41.728874	15
CCCGCAC	4705	0.0	41.649307	32
CGCAAGC	5345	0.0	41.56782	19
CTTAATC	5850	0.0	41.51795	10
AACGCAA	5390	0.0	41.47681	17
CAACGCA	5400	0.0	41.442593	16
GCTTATG	5225	0.0	41.33397	24
GACTTAA	5850	0.0	41.321365	8
CTTATGA	5215	0.0	41.280922	25
>>END_MODULE